首页|Simple and programmed three-dimensional DNA tweezer for simultaneous one-step detection of ochratoxin A and zearalenone
Simple and programmed three-dimensional DNA tweezer for simultaneous one-step detection of ochratoxin A and zearalenone
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NSTL
Elsevier
Three-dimensional (TD) deoxyribonucleic acid (DNA) tweezers were programmed for one-step identification and detection of ochratoxin A (OTA) and zearalenone (ZEN). The unfolding of the TD-DNA tweezers by aptamers specific to these two mycotoxins "turned " the fluorescent signals "on. " The bonding of the aptamers to their corresponding targets in OTA and ZEN "turned " the fluorescent signals and the DNA tweezers "off. " The detection limit of the TD-DNA tweezers for OTA and ZEN was 0.032 and 0.037 ng mL(-1), respectively. The feasibility of this method was tested using two samples. Detection via this method increased the recovery of OTA and ZEN from 95.8% to 110.2%. Spike recovery and certified food products were used to detect applicability in actual situations. Analyte detection in complex samples using TD-DNA tweezers is rapid, as the process involves a single operational step. This proposed design has considerable potential for application in mycotoxin detection. (c) 2022 Elsevier B.V. All rights reserved.
DNA tweezerMycotoxinSimultaneous detectionAptamerOne-step detectionGenetic techniqueMYCOTOXINSNANOCAGESTOXICITY
NSTL
Elsevier
