首页|New spirit of an old technique: Characterization of lipid phase transitions via UV/Vis spectroscopy

New spirit of an old technique: Characterization of lipid phase transitions via UV/Vis spectroscopy

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  • NSTL
  • Elsevier
One of the advantages of investigating lipid phase transitions by thermoanalytical techniques such as DSC is manifested in the proportionality of the signal strength on a DSC curve, attributed to a particular thermotropic event, and its cooperativity degree. Accordingly, the pretransition of 1,2-dipalmitoyl-sn- glycero-3-phosphocholine (DPPC) is less noticeable than its main phase transition; as a matter of fact, when DSC measurements are performed at low heating rate, such low-cooperativity phase transition could go (almost) unnoticed. The aim of this work is to present temperature-dependent UV/Vis spectroscopy, based on a temperature-dependent change in DPPC suspension turbidity, as a technique applicable for determination of lipid phase transition temperatures. Multivariate analyzes of the acquired UV/ Vis spectra show that phase transitions of the low-cooperativity degree, such as pretransitions, can be identified with the same certainty as transitions of a high-cooperativity degree.(c) 2022 Elsevier B.V. All rights reserved.

UVVis spectroscopy12-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)CholesterolLipid phase transitionsDifferential spectral analysis (DSA)Multivariate curve analysis (MCA)MULTIVARIATE CURVE RESOLUTIONX-RAY-DIFFRACTIONRIPPLE PHASETEMPERATURE-DEPENDENCEWATER PERMEATIONCHAIN-LENGTHP-BETABILAYERSPHOSPHATIDYLCHOLINEDIPALMITOYLPHOSPHATIDYLCHOLINE

Males, Petra、Brkljaca, Zlatko、Jurasin, Darija Domazet、Bakaric, Danijela

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Rudjer Boskovic Inst

2022

10.1016/j.saa.2022.121013

Spectrochimica acta

Spectrochimica acta

ISSN：1386-1425
年,卷(期)：2022.272
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