首页|Electrochemiluminescence biosensor for determination of lead(II) ions using signal amplification by Au@SiO2 and tripropylamine-endonuclease assisted cycling process
Electrochemiluminescence biosensor for determination of lead(II) ions using signal amplification by Au@SiO2 and tripropylamine-endonuclease assisted cycling process
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
MXene@Au as the base and Au@SiO2 as signal amplification factor were used for constructing an ultrasensitive"on-off'electrochemiluminescence(ECL) biosensor for the detection of Pb~(2+) in water.The use of MXene@Au composite provided a good interface environment for the loading of tris(2,2-bipyridyl)ruthenium(II)(Ru(bpy)3~(2+)) on the electrode.Based on resonance energy transfer,the Au(core) SiO2(shell)(Au@SiO2) nanoparticles stimulate electron transport and promote tripropylamine(TPrA) oxidation.The luminescence effect of Au@SiO2 was five times that of AuNPs and SiO2 nanomaterials alone,and the ECL intensity was greatly improved.In addition,Pb~(2+) activated the aptamer to exert its endonuclease activity,which realized the signal cycle amplification in the process of Pb~(2+) detection.When Pb~(2+) was added,the ECL signal weakened,and the Pb~(2+) concentration was detected according to the decreased ECL intensity.Under optimized experimental conditions,this aptamer sensor for Pb~(2+) has a wide detection range(0.1 to 1 × 10~6 ng L~(-1)) and a low detection limit(0.059 ng L~(-1)).The relative standard deviation(RSD) of the sensor is 0.39-0.99%,and the recovery of spiked standard is between 90.00 and 125.70%.The sensor shows good selectivity and high sensitivity in actual water sample analysis.This signal amplification strategy possibly provides a new method for the detection of other heavy metal ions and small molecules.
NSTL
