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Mikrochimica Acta
Springer Verlag
Mikrochimica Acta

Springer Verlag

0026-3672

Mikrochimica Acta/Journal Mikrochimica ActaSCIISTPAHCI
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    Au-doped MOFs catalyzed electrochemiluminescence platform coupled with target-induced self-enrichment for detection of synthetic cannabinoid RCS-4

    Qianying CaoDing JiangFangmin Xu
    9页
    查看更多>>摘要：A ternary composite material with Au,Co-based organic frameworks(ZIF-67) and perylene derivatives(PTCD-cys) has been synthesized for identification of synthetic cannabinoids.Through contact with Au-S,Au-ZIF-67 increased electrochemiluminescence(ECL) sensitivity and stability and efficiently catalyzed the ECL of PTCD-cys.Compared with the ECL response of PTCD-cys monomer,the ECL signal value of the composite material was significantly increased,and the onset potential of Au-ZIF-67/PTCD-cys favorably shifted more than that of PTCD-cys/GCE.When the target cannabinoid molecule RCS-4 appeared,Au-ZIF-67 captured and immobilized it on the sensor surface by adsorption to achieve target-induced self-enrichment of RCS-4.Under optimal conditions,the ECL sensor was found to be linearly related to the logarithm of the RCS-4 concentration ranging from 3.1 × 1CT15 to 3.1 × 10~9 mol/L with a detection limit(LOD) of 6.0× 10~16 mol/L(S/N = 3).The approach had the advantages of being simple to use,having a high sensitivity,a wide detection range,and good stability,making it a novel platform for RSC-4 detection in public health safety monitoring.
      原文链接:
    • NSTL

    Peroxidase-like activity of bimetal Cu-Zn oxide mesoporous nanospheres for the determination of o-aminophenol

    Yuji QiYulian TangXuemei Zhou
    10页
    查看更多>>摘要：To enhance the peroxidase-like performance and its application in detection of toxic o-aminophenol(o-AP),a kind of bimetal Cu-Zn oxide-based mesoporous nanosphere(Cu_(2/3)Zn_(1/3)O PNPs) was constructed under microwave-radiation conditions.Its mesoporous microstructure and peroxidase-like catalytic activity were investigated in detail.The results showed that Cu_(2/3)Zn_(1/3)O PNPs possessed a high specific surface area of 34.89 m~2g~(-1) and a well-distributed mesoporous size of approximate 6.07 nm,which endowed the superior peroxidase-like performance.The material catalyzes the oxidization of 3,3',5,5'-tetramethylbenzidine(TMB) with Km/V_(max) of 0.104 mM/3.79 × 10~(-8)M·s~(-1) in the presence of H2O2.Especially o-AP could exclusively deteriorate the characteristic UV-Vis absorbance intensity at 653 nm(A_(653)) of the Cu_(2/3)Zn_(1/3)O PNPs-TMB-H2O2 system with obvious color change from blue to colorless.Under the optimal conditions,the effect of some interfering substances was low and the limit of detection(LOD) for o-AP was 1.65 × 10~(-8) mol/L(S/N = 3).When applied to the colorimetric detection of o-AP in practice,the recovery was between 96.1 and 107.2% with R.S.D.less than 2.04%.The mechanism of synergic-enhancement peroxidase-mimic activity of Cu_(2/3)Zn_(1/3)O PNPs and its exclusive colorimetric response to o-AP were proposed as well.
      原文链接:
    • NSTL

    Three-dimensional manganese cobaltate:a highly conductive electrocatalystfor paraoxon-ethyl detection

    Thangavelu KokulnathanTzyy-Jiann WangYing-Yu Wang
    10页
    查看更多>>摘要：The synthesis of manganese cobaltate(MnCo2O4) with the hybrid three-dimensional architecture has been developed as an electrocatalyst for the electrochemical sensing of paraoxon-ethyl(PEL).The detailed physicochemical and structural characterization of MnCo2O4 is meticulously examined.The MnCo2O4-modified screen-printed carbon electrode(SPCE) exhibits good electrocatalytic activity for the reduction of PEL compared with the bare SPCE due to numerous unique properties.By profiting from these advantages,the proposed MnCo2O4/SPCE shows superior sensing performance toward the determination of PEL,including low cathodic peak potential(-0.64 V),wide detection range(0.015-435 μM),low limit of detection(0.002 μM),high detection sensitivity(2.30 μA μM~(-1) cm~(-2)),excellent selectivity,and good reproducibility.Notably,the electrochemical performance of the MnCo2O4-based electrocatalyst is superior to those previously reported in the literatures.The practical application of the MnCo2O4/SPCE is effectively assessed in the analysis of food and water samples with satisfied recoveries of 96.00-99.35%.The superior performance of the proposed MnCo2O4 electrocatalyst holds considerable potential for future development of electrochemical sensing platforms.
      原文链接:
    • NSTL

    Non-invasive detection of COVID-19 using a microfluidic-based colorimetric sensor array sensitive to urinary metabolites

    Mohammad Mahdi BordbarHosein SamadiniaAzarmidokht Sheini
    11页
    查看更多>>摘要：A colorimetric sensor array designed on a paper substrate with a microfluidic structure has been developed.This array is capable of detecting COVID-19 disease by tracking metabolites of urine samples.In order to determine minor metabolic changes,various colorimetric receptors consisting of gold and silver nanopartides,metalloporphyrins,metal ion complexes,and pH-sensitive indicators are used in the array structure.By injecting a small volume of the urine sample,the color pattern of the sensor changes after 7 min,which can be observed visually.The color changes of the receptors(recorded by a scanner) are subsequently calculated by image analysis software and displayed as a color difference map.This study has been performed on 130 volunteers,including 60 patients infected by COVID-19,55 healthy controls,and 15 cured individuals.The resulting array provides a fingerprint response for each category due to the differences in the metabolic profile of the urine sample.The principal component analysis-discriminant analysis confirms that the assay sensitivity to the correctly detected patient,healthy,and cured participants is equal to 73.3%,74.5%,and 66.6%,respectively.Apart from COVID-19,other diseases such as chronic kidney disease,liver disorder,and diabetes may be detectable by the proposed sensor.However,this performance of the sensor must be tested in the studies with a larger sample size.These results show the possible feasibility of the sensor as a suitable alternative to costly and time-consuming standard methods for rapid detection and control of viral and bacterial infectious diseases and metabolic disorders.
      原文链接:
    • NSTL

    Electrochemiluminescence biosensor for determination of lead(II) ions using signal amplification by Au@SiO2 and tripropylamine-endonuclease assisted cycling process

    Hongguo ZhaiYue WangJiaqi Yin
    11页
    查看更多>>摘要：MXene@Au as the base and Au@SiO2 as signal amplification factor were used for constructing an ultrasensitive"on-off'electrochemiluminescence(ECL) biosensor for the detection of Pb~(2+) in water.The use of MXene@Au composite provided a good interface environment for the loading of tris(2,2-bipyridyl)ruthenium(II)(Ru(bpy)3~(2+)) on the electrode.Based on resonance energy transfer,the Au(core) SiO2(shell)(Au@SiO2) nanoparticles stimulate electron transport and promote tripropylamine(TPrA) oxidation.The luminescence effect of Au@SiO2 was five times that of AuNPs and SiO2 nanomaterials alone,and the ECL intensity was greatly improved.In addition,Pb~(2+) activated the aptamer to exert its endonuclease activity,which realized the signal cycle amplification in the process of Pb~(2+) detection.When Pb~(2+) was added,the ECL signal weakened,and the Pb~(2+) concentration was detected according to the decreased ECL intensity.Under optimized experimental conditions,this aptamer sensor for Pb~(2+) has a wide detection range(0.1 to 1 × 10~6 ng L~(-1)) and a low detection limit(0.059 ng L~(-1)).The relative standard deviation(RSD) of the sensor is 0.39-0.99%,and the recovery of spiked standard is between 90.00 and 125.70%.The sensor shows good selectivity and high sensitivity in actual water sample analysis.This signal amplification strategy possibly provides a new method for the detection of other heavy metal ions and small molecules.
      原文链接:
    • NSTL

    Nanomaterials-based electrochemical sensors for the detection of natural antioxidants in food and biological samples:research progress

    Haoye WangShixin JiangJie Pan
    44页
    查看更多>>摘要：Antioxidants are healthy substances that are beneficial to the human body and exist mainly in natural and synthetic forms.Among many kinds of antioxidants,the natural antioxidants have great applications in many fields such as food chemistry,medical care,and clinical application.In recent years,many efforts have been made for the determination of natural antioxidants.Nano-electrochemical sensors combining electrochemistry and nanotechnology have been widely used in the determination of natural antioxidants due to their unique advantages.Therefore,a large number of nanomaterials such as metal oxide,carbon materials,and conducting polymer have attracted much attention in the field of electrochemical sensors due to then good catalytic effect and stable performance.This review mainly introduces the construction of electrochemical sensors based on different nanomaterials,such as metallic nanomaterials,metal oxide nanomaterials,carbon nanomaterials,metal-organic frameworks,polymer nanomaterials,and other nanocomposites,and their application to the detection of natural antioxidants,including ascorbic acid,phenolic acids,flavonoid,tryptophan,citric acid,and other natural antioxidants.In the end,the limitations of the existing nano-sensing technology,the latest development trend,and the application prospect for various natural antioxidant substances are summarized and analyzed.We expect that this review will be helpful to researchers engaged in electrochemical sensors.
      原文链接:
    • NSTL

    SARS-CoV-2 spike protein detection using slightly tapered no-core fiber-based optical transducer

    Jinsil HanSeul-Lee LeeJihoon Kim
    13页
    查看更多>>摘要：The label-free detection of SARS-CoV-2 spike protein is demonstrated by using slightly tapered no-core fiber(ST-NCF) functionalized with ACE2.In the fabricated sensor head,abrupt changes in the mode-field diameter at the interfaces between single-mode fiber and no-core fiber excite multi-guided modes and facilitate multi-mode interference(MMI).Its slightly tapered region causes the MMI to be more sensitive to the refractive index(RI) modulation of the surrounding medium.The transmission minimum of the MMI spectrum was selected as a sensor indicator.The sensor surface was functionalized with ACE2 bioreceptors through the pretreatment process.The ACE2-immobilized ST-NCF sensor head was exposed to the samples of SARS-CoV-2 spike protein with concentrations ranging from 1 to 10~4 ng/mL.With increasing sample concentration,we observed that the indicator dip moved towards a longer wavelength region.The observed spectral shifts are attributed to localized RI modulations at the sensor surface,which are induced by selective bioaffinity binding between ACE2 and SARS-CoV-2 spike protein.Also,we confirmed the capability of the sensor head as an effective and simple optical probe for detecting antigen protein samples by applying saliva solution used as a measurement buffer.Moreover,we compared its detection sensitivity to SARS-CoV-2 and MERS-CoV spike protein to examine its cross-reactivity.In particular,we proved the reproducibility of the bioassay protocol adopted here by employing the ST-NCF sensor head reconstructed with ACE2.Our ST-NCF transducer is expected to be beneficially utilized as a low-cost and portable biosensing platform for the rapid detection of SARS-CoV-2 spike protein.
      原文链接:
    • NSTL

    Smartphone-based paper microfluidic competitive immunoassay for the detection of α-amanitin from mushrooms

    Yan LiangAvory ZhouCandace S.Bever
    13页
    查看更多>>摘要：α-Amanitin is of ten considered the most poisonous mushroom toxin produced by various mushroom species,which are hard to identify from edible,non-toxic mushrooms.Conventional detection methods require expensive and bulky equipment or fail to meet high analytical sensitivity.We developed a smartphone-based fluorescence microscope platform to detect α-amanitin from dry mushroom tissues.Antibody-nanoparticle conjugates were captured by immobilized antigen-hapten conjugates while competing with the free analytes in the sample.Captured fluorescent nanoparticles were excited at 460 nm and imaged at 500 nm.The pixel numbers of such nanoparticles in the test zone were counted,showing a decreasing trend with increasing analyte concentration.The detection method exhibited a low detection limit(1 pg/mL),high specificity,and selectivity,allowing us to utilize a simple rinsing for toxin extinction and avoiding the need for high-speed centrifugation.In addition,this assay's short response time and portable features enable field detection of α-amanitin from amanitin-producing mushrooms.
      原文链接:
    • NSTL

    Electrochemiluminescence resonance energy transfer system between ruthenium-based nanosheets and CdS quantum dots for detection of chlorogenic acid

    Lin WangBeibei WangKai Kang
    11页
    查看更多>>摘要：A new strategy is proposed for ultrasensitive detection of chlorogenic acid(CGA) by fabricating an electrochemiluminescence resonance energy transfer(ECL-RET) sensing platform.The novel system designed by introducing ruthenium-based 2D metal-organic framework nanosheets(Ru@Zn-MOF) as ECL acceptor and L-cysteine capped CdS quantum dots(L-CdS QDs) as ECL donor,exhibited good ECL response.The possible mechanism of the modified electrode surface reaction was discussed.Modifying of the electrode surface by application of L-CdS QDs directly on ultrathin MOF nanosheets greatly shortened the electron-transfer distance and reduce energy loss,therefore significantly improving the ECL efficiency.The prepared sensor demonstrated good stability and highly selective detection of the target molecule.Under optimal conditions,the constructed sensor for the detection of CGA exhibited a wide linear range from 1.0 × 10~(-10) to 1.0 × 10~(-4) mol·L~(-1) and a low detection limit of 3.2 × 10~(-11) mol·L~(-1) with a correction coefficient of 0.995.The recovery for spiked samples was calculated to be 94.4-109% and the RSD was 1.07-1.72% in real samples.The obtained sensor is considered to be a promising platform for CGA detection.
      原文链接:
    • NSTL

    Determination of total cathinones with a single molecularly imprinted fluorescent sensor assisted by electromembrane microextraction

    Rong HuYibo YanLong Jiang
    12页
    查看更多>>摘要：An electromembrane microextraction(EME)-assisted fluorescent molecularly imprinted polymer(MIP) sensing method is presented for detecting the total cathinone drugs in urine samples.In this detection system,the clean-up ability of EME eliminated the matrix effects on both target binding with MIPs and the luminescence of the fluorophore in the sensor.Moreover,by optimizing the extraction conditions of EME,different cathinone drugs with a same concentration show a same response on the single aggregation induced emission(AIE) based MIP(AIE-MIP) sensor(λ_(cx) = 360 nm,λ_(cm) = 467 nm).The recoveries were 57.9% for cathinone(CAT) and 78.2% for methcathinone(MCAT).The EME-assisted"light-up"AIE-MIP sensing method displayed excellent performance with a linear range of 2.0-12.0 μmol L~(-1) and a linear determination coefficient(R~2) of 0.99.The limit of detection(LOD) value for EME-assisted"light-up"AIE-MIP sensing method was 0.3 umol L~(-1).The relative standard deviation(RSD) values for the detection were found to be within the range 2.0-12.0%.To the best of our knowledge,this is the first time that determination of total illicit drugs with a single fluorescent MIP sensor was achieved and also the first utilization of sample preparation to tune the sensing signal of the sensor to be reported.We believe that this versatile combination of fluorescent MIP sensor and sample preparation can be used as a common protocol for sensing the total amount of a group of analytes in various fields.
      原文链接:
    • NSTL