首页|A method for generating virus-free cassava plants to combat viral disease epidemics in Africa

A method for generating virus-free cassava plants to combat viral disease epidemics in Africa

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  • NSTL
  • Elsevier
Here, we report a method to clean cassava plants from viral infections that cause cassava mosaic and brown streak diseases in Africa. Infected plants of resistant or tolerant varieties from Malawi, Mozambique, Kenya, Tanzania and Uganda were cleaned in the UK using a combination of tissue culture, chemotherapy and thermotherapy. In the first cycle of our virus-indexing procedure, we successfully cleaned 27 of the 31 varieties (87%), and after an additional three cleaning cycles, all plants were virus-free. Virus-free tissue-cultured plants were shipped back to Africa for distribution to farmers. This first cross-boundary effort provides important lessons for mitigating the two-major cassava viral diseases.

Manihot esculentaCassava brown streak diseaseCassava mosaic diseaseVirus indexingVirus diagnosis

Whitfield, E. Charles、Otti, Gerald、Tumwegamire, Silver、Kanju, Edward、Legg, James P.、Mkamilo, Geoffrey、Kawuki, Robert、Benesi, Ibrahim、Zacarias, Anabela、Munga, Therezia、Mwatuni, Francis、Mbugua, Edward、Maruthi, M. N.

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Univ Greenwich, NRI, Chatham ME4 4TB, Kent, England

IITA, POB 34441, Dar Es Salaam, Tanzania

Dept Agr Res & Dev DRD, POB 2066, Dar Es Salaam, Tanzania

NARO, POB 295, Entebbe, Uganda

Chitedze Res Stn, DARS, POB 158, Lilongwe, Malawi

Natl Inst Agr Res IIAM, POB 3658, Maputo, Mozambique

KALRO, Nairobi, Kenya

Kenya Plant Inspectorate Serv KEPHIS, Plant Quarantine & Biosecur Stn, POB 49592 00100, Nairobi, Kenya

GTIL, POB 47430-00100, Nairobi, Kenya

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2019

10.1016/j.pmpp.2018.09.002

Physiological and Molecular Plant Pathology

Physiological and Molecular Plant Pathology

SCI
ISSN：0885-5765
年,卷(期)：2019.105
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