首页|A copper nanoclusters probe for dual detection of microalbumin and creatinine

A copper nanoclusters probe for dual detection of microalbumin and creatinine

扫码查看
原文链接
  • NSTL
  • Elsevier
A fluorescent probe based on glutathione-capped copper nanoclusters (GSH-CuNCs) was developed for the detection of dual targets, human serum albumin (HSA) and creatinine, in human urine. The GSH-CuNCs were synthesized by a one-pot green method using ascorbic acid as a reducing agent. The detection of HSA was in a turn-on mode via electrostatic interaction in a basic condition while the detection of creatinine was in a turn-off mode via non-covalent bonding in an acidic condition. Under optimal conditions, the linear range and detection limit of HSA were 5.0 nM to 150 nM and 1.510 +/- 0.041 nM, while those of creatinine were 30 mu M to 1000 mu M and 13.0 +/- 1.0 mu M. This easily fabricated nanocluster probe provided a fast response with high sensitivity, and good selectivity. Recoveries from urine samples were in the range of 81.44 +/- 0.25 to 109.22 +/- 0.57% for HSA and 80.57 +/- 0.16 to 109.0 +/- 0.10% for creatinine. The urinary analytical results from the fluorescent probe were in good agreement (P > 0.05) to those obtained from immunoturbidimetric and enzymatic methods, signifying the excellent performance of this sensing system. (C) 2021 Elsevier B.V. All rights reserved.

GlutathioneCopper nanoclustersHuman serum albuminCreatinineAlbumin to creatinine ratioFluorescence detectionCHRONIC KIDNEY-DISEASEHUMAN SERUM-ALBUMINGOLD NANOCLUSTERSCARBON NANOTUBESGREEN SYNTHESISASCORBIC-ACIDFLUORESCENTBIOSENSORCRYOGELSENSOR

Thammajinno, Supitcha、Buranachai, Chittanon、Kanatharana, Proespichaya、Thavarungkul, Panote、Thammakhet-Buranachai, Chongdee

展开 >

Prince Songkla Univ

2022

10.1016/j.saa.2021.120816

Spectrochimica acta

Spectrochimica acta

ISSN：1386-1425
年,卷(期)：2022.270
  • 9
  • 67