首页|Transcriptome and biochemical analyses of rainbow trout (Oncorhynchus mykiss) RTG-2 gonadal cells in response to BDE-47 stress indicates effects on cell proliferation
Transcriptome and biochemical analyses of rainbow trout (Oncorhynchus mykiss) RTG-2 gonadal cells in response to BDE-47 stress indicates effects on cell proliferation
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NSTL
Elsevier
2,2',4,4'-Tetrabromodiphenyl ether (BDE-47) is a biotoxin of polybrominated diphenyl ether (PBDEs) frequently detected in the environment. Apoptosis and cell cycle arrest are important toxic phenomena of xenobiotics that inhibit cell proliferation. In this study, we investigated the effects of BDE-47 (5 mu M, 10 mu M, 20 mu M, 40 mu M) on cell viability, morphology, cell cycle and apoptosis. BDE-47 significantly decreased cell viability, and morphological alterations were observed. The significant increase in cells at G1 phase indicated the occurrence of G1 phase cell cycle arrest in RTG-2 cells. An acridine orange and ethidium bromide (AO/EB) staining assay was employed and revealed the induction of apoptosis in RTG-2 cells. The results indicated that BDE-47 exposure inhibits cell proliferation. Transcriptome analysis was applied for further evidence. A total of 1300 differentially expressed genes (DEGs) were identified in RTG-2 cells, among which 26 DEGs were associated with the cell cycle and apoptosis. Western blotting and qPCR analyses also showed the expression of cell cycle- and apoptosis-related proteins and genes. Mapping the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, p53, Tumor necrosis factor (TNF), Mitogen-activated protein kinase (MAPK), phosphatidylinositide 3-kinase-AKT (PI3K-AKT), and reaction oxygen species (ROS)-mediated signaling pathways were determined to be the major pathways involved in modulating the cell cycle and apoptosis. Since we demonstrated simultaneous ROS overproduction during BDE-47 exposure in a previous study, we speculated a possible explanation for the observation: BDE-47-induced ROS overproduction was the initiating signal, which activated cell cycle arrest and apoptosis and finally inhibited cell proliferation.
NSTL
Elsevier
