首页|Kinetic effect of oestrogen on secretion of prostaglandins E2 and F2α in bovine oviduct epithelial cells

Kinetic effect of oestrogen on secretion of prostaglandins E2 and F2α in bovine oviduct epithelial cells

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  • NSTL
  • Csiro Publishing
This study aimed to investigate the effect of oestrogen on prostaglandin E2 (PGE_2) and prostaglandin F2α (PGF_(2α)) secretion in bovine oviduct epithelial cells. Bovine oviduct epithelial cells were obtained from the lumen of fresh bovine oviducts. Quantitative real-time polymerase chain reaction and in-cell western assays were used to measure PGE_2 and PGF_(2α) synthase activity and enzyme-linked immunosorbent assays were used to detect the concentrations of the two prostaglandins in extracellular fluid. We observed that oestradiol caused a short-term increase in cyclo-oxygenase-2 (COX-2), which stimulated PGE_2 and PGF_(2α) secretion, and that a subsequent decrease in COX-2 and an increase in cyclooxygenase-1 (COX-1 ) produced a high PGE_2 : PGF_(2α) ratio. These findings reflect the dynamic change in PGE_2 and PGF_(2α) levels under the influence of oestrogen, which may be essential for fertilisation.

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Zhiheng Dong、Nan Zhang、Wei Mao、Bo Liu、Na Huang、Peifeng Li、Changyou Li、Jinshan Cao

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Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan Distri

2017

10.1071/RD15246

Theoretical and Experimental Plant Physiology

Theoretical and Experimental Plant Physiology

ISSN：
年,卷(期)：2017.29(3)
  • 31