首页|RNA sequencing reveals that cell wall, Ca2+, hypersensitive response and salicylic acid signals are involved in pear suspension cells responses to Valsa pyri infection
RNA sequencing reveals that cell wall, Ca2+, hypersensitive response and salicylic acid signals are involved in pear suspension cells responses to Valsa pyri infection
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NSTL
Elsevier
? 2022Pear, one of the staple fruit crops in the world, is under threat from Valsa pyri (Vp) infection. Investigations of the molecular mechanisms underlying pear resistance against Vp infection are largely limited. Using RNA sequencing, we investigated the global gene expression profiles of suspension cells from the resistant rootstock ‘Duli-03′ (Pyrus betulifolia Bunge) and a susceptible cultivar ‘Yuluxiang’ (P. bretschneideri Rehd.) in response to Vp infection. Differentially expressed genes (DEGs) mainly involved in “Cell wall biosynthesis”, “Signal transduction”, “Plant–pathogen interaction”, “Plant hormone signal transduction”, etc., were studied. Further investigations revealed that genes associated with cell wall, Ca2+, hypersensitive response (HR), defense response, and salicylic acid (SA) participated in pear responses to Vp signals. Functional analysis revealed that defense-related genes PbeKCS1/10-1 and PbeMPK4-1 positively regulated Vp resistance in pear fruit, whereas four TGACG motif binding factor (TGA) genes played a negative regulatory role.
Agrobacterium infiltrationGene cloning and verificationHypersensitive responseqRT-PCRRNA sequencingValsa pyri
Duo H.、Yu H.、Sun E.、Zhao D.、Zuo C.
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College of Horticulture Gansu Agricultural University
NSTL
Elsevier
