首页|ISOLATION AND CHARACTREIZATION OF MICROSOMAL omega-6 DESATURASE GENE FROM GLYCINE MAX L. CV. PUSA -9702

ISOLATION AND CHARACTREIZATION OF MICROSOMAL omega-6 DESATURASE GENE FROM GLYCINE MAX L. CV. PUSA -9702

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Using gene specific primers, a genomic sequence encoding omega-6 desaturase gene (fad2-l) of soybean was PCR amplified, cloned and analyzed for sequence homology. This partial genomic sequence is 191 bp long and untranslated region but has no intronsand 5' untranslated region. This partial gene fragment can be used for screening soybean genomic cDNA libraries for isolation of complete gene.

Cloninglibrary screeningPCR amplificationfad (fatty acid desaturase)untranslated region

RAJESH KUMAR、NIRUPAMA DUBEY、ARCHANA SACHDEV

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Division of Biochemistry, Indian Agricultural Research Institute, New Delhi-110012

2008

New Botanist

New Botanist

ISSN:0377-1741
年,卷(期):2008.35(1/4)