首页|Role of silver nanoparticles in fluorimetric determination of urea in urine samples
Role of silver nanoparticles in fluorimetric determination of urea in urine samples
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NSTL
Elsevier
Herein, an economical, analytical and sensitive method was established for the fluorometric determination of urea using freshly prepared silver nanoparticles (Ag-NPs) in real urine samples. The standard addition and second-order derivative methods were selected for the ongoing research work to eliminate the possible effect of interferences in a real environment. In this work, Ag-NPs were prepared by reducing silver nitrate salt in the presence of 1,3-di-(1H-imidazole-1-yl) -2-propanol (DIPO) in an aqueous medium. Urea in the urine samples was successfully determined through the complexation of Ag-NPs with urea molecules. The results revealed high percent recovery with +/- RSD of urea in the three different urine samples, where percent recoveries by spectrofluorometric standard addition were 99.77 +/- 3.4, 100.24 +/- 5. 1, 100.93 +/- 2.8 and that is by the spectrofluorometric second-order derivative method were 103.57 +/- 2.4, 101.8 +/- 1.3, 98 +/- 3.2, respectively. The successful application of these analytical methods in the spectrofluorometric determination of urea in urine samples can accumulate further addition in the effects and possible role of Ag-NPs in the determination of biological molecules in biological and nonbiological samples in the scientific as well as clinical fields. (C) 2022 Elsevier B.V. All rights reserved.
Silver nanoparticlesUreaFluorometric determinationStandard addition methodSecond order derivative methodSTANDARD ADDITIONSPECTROSCOPYSIZEULTRAVIOLETSURFACTANTSENSOR
Zahid, Muhammad、Khan, Muhammad Farooq、Ismail, Muhammad、Wang Xiangke、Cazzato, Gerardo、Saleemi, Hassan Anwar、Khan, Ayub、Ismail, Ahmed
NSTL
Elsevier
