首页|The FvemiR167b-FveARF6 module increases the number of roots and leaves in woodland strawberry
The FvemiR167b-FveARF6 module increases the number of roots and leaves in woodland strawberry
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NSTL
Elsevier
Roots and leaves are both important organs of the plant and central to the life strategy, which are crucial for plant survival and perform a wide range of functions. However, the mechanism of miR167 and its target gene, auxin response factor6 (ARF6), regulating the development of roots and leaves for strawberry, has not yet been characterized. In this study, miR167b precursor and FveARF6 were isolated from the woodland strawberry 'Ruegen' (Fragaria vesca), and RLM-5 ' RACE showed that FveARF6 transcript is cleavage target of FvemiR167b. Both FvemiR167b and its target gene expressed in different organs of strawberry, and the expression of FveARF6 could be induced by IAA. Overexpressing-FvemiR167b and RNAi-FveARF6 transgenic lines both exhibited an increase in the number of roots and leaves of strawberry. Transactivation analysis showed that FveARF6 presented transcriptional activation activity in yeast. Gene expression analysis indicated that the transcript levels of the strawberry GH3 family also declined in RNAi-ARF6 transgenic plants compared with the wild-type controls. The GH3 family promoter sequences of strawberry had at least one AuxRE element. Taken together, our results reveal that FvemiR167b represses the expression of FveARF6 gene to cause the roots and leaves of strawberry to grow in number, which can likely further regulate the growth of the plant by GH3 family. This will help us understand the molecular mechanism of miR167-ARF6 module in woodland strawberry.
NSTL
Elsevier
