首页|Assessment of high hydrostatic pressure for the cryopreservation procedure of boar semen with low initial sperm motility
Assessment of high hydrostatic pressure for the cryopreservation procedure of boar semen with low initial sperm motility
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NSTL
Cryopreservation of biological material is critical for programmes of animal conservation and provides insurance for calamities, such as the loss of breeds due to animal diseases. The creation of biological material cryocollections depends on an effective cryopreservation procedure. The treatment of spermatozoa using high hydrostatic pressure (HHP) before the freezing procedure is a technological solution increasing cryopreservation efficiency. Our previous study demonstrated that HHP treatment (Applied Cell Technology, Hungary) with 35 MPa at 21 degrees C for 1.5 h prior to boar semen cryopreservation improves the quality of post-thaw spermatozoa. This study aimed to evaluate the effect of fresh boar semen HHP treatment on the post-thaw sperm parameters. Only ejaculates with sperm progressive motility (PM%) below 70% (7 boars, 4-6 ejaculates/boar) were used in the experiment. All ejaculates (control samples without HHP treatment and samples treated with 35 MPa at 21 degrees C for 1.5 h) were cryopreserved using a patented method (no. PL 228192). The results showed that post-thaw sperm motility (TM%) of the HHP-treated sperm was significantly higher (P<0.05; P<0.01) than that of the control sperm (43.0 vs. 37.5%; 58.9 vs. 54.9%, 40.1 vs. 35.5%; and 57.7 vs. 51.0% in boars nos. I, II, IV, and V, respectively). There were no significant differences (P>0.01) in the percentage of sperm displaying DNA fragmentation after cryopreservation between the treated and untreated samples. The analysis of ejaculates from three boars revealed a significantly higher (P<0.05) percentage of viable sperm (YO-PRO-1-/PI-) in the HHP-treated samples than in the control samples (37.2 vs. 32.0%; 40.5 vs. 33.8% and 54.7 vs. 46.6%). Our study demonstrates that the HHP treatment applied in the freezing procedure of boar semen with initial sperm motility below 70% protects spermatozoa against cryodamage. However, the increase in semen tolerance to the cryopreservation procedure is an individual predisposition of specific boars.
NSTL
