首页|A turn on fluorescent assay for real time determination of beta-galactosidase and its application in living cell imaging
A turn on fluorescent assay for real time determination of beta-galactosidase and its application in living cell imaging
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NSTL
Elsevier
In recent years, fluorescent probes based on chemical reactions have been widely investigated as a powerful and noninvasive method for the diagnosis of diseases. beta-Galactosidase (beta-gal), a typical lysosomal glycosidase, over expressed in senescent cells and primary ovarian cancer cells, which has been considered as an important biomarker cell senescence and primary ovarian cancers. Fluorescent probes for the determination of beta-gal provide an excellent choice for visualization of cell senescence. In this work, a turn on fluorescent probe (HBT-gal) for beta-gal activity was developed based on the enzymatic hydrolysis of glycosidic bonds. HBT-gal showed little fluorescence in aqueous buffer excited at 415 nm, while emitted green fluorescence centered at similar to 492 nm upon incubated with beta-gal. The sensing scheme showed high selectivity and sensitivity for beta-gal activity with a limit of detection calculated as low as 0.19 mU/mL. Moreover, HBT-gal was successfully applied to image beta-gal activity in senescent Hep G2 cells treated with H2O2. Therefore, probe HBT-gal demonstrated a potential usage for the determination of cell senescence using beta-gal as a biomarker. (C) 2021 Elsevier B.V. All rights reserved.
beta-galactosidaseFluorescence receptorReal time detectionSenescent cellsBiological imagingSELECTIVE DETECTIONENZYME-ACTIVITYPROBEVISUALIZATIONTRACKINGAIE
NSTL
Elsevier
