首页|Cascade amplification strategy combined with analyte-triggered fluorescence switching of dual-quenching system for highly sensitive detection of isoniazide
Cascade amplification strategy combined with analyte-triggered fluorescence switching of dual-quenching system for highly sensitive detection of isoniazide
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NSTL
Elsevier
A sensitive fluorescence sensing platform consisting of manganese dioxide nanosheets (MnO2) and gold nanoparticles (AuNPs) as dual nanoquenchers has been constructed to detect isoniazid combined with analyte-triggered cascade reactions. The fluorescence of 2,3-diaminophenazine (DAP) is quenched simultaneously by MnO2 and AuNPs via inner filter effect. MnO2 is decomposed by isoniazid to generate Mn2+, which makes AuNPs aggregated. The quenching abilities of both the decomposed MnO2 and aggregated AuNPs are inhibited, causing remarkable fluorescence recovery. The usage of dual nanoquenchers enhances the quenching efficiency and reduces the fluorescence background. Moreover, the isoniazidtriggered cascade reaction further amplifies the readout signal. Thus, this strategy exhibits higher sensitivity towards the detection of isoniazid. Compared with MnO2-based fluorescence assay, this strategy possesses lower limit of detection. This strategy has been successfully used to detect isoniazid in pharmaceutical preparations, which is of great significance for drug analysis. (C) 2022 Elsevier B.V. All rights reserved.
NSTL
Elsevier
