首页|ISOLATION AND CHARACTERIZATION OF PROTEASE INHIBITOR FROM LENTIL (LENS CULINARLS MEDIK.)
ISOLATION AND CHARACTERIZATION OF PROTEASE INHIBITOR FROM LENTIL (LENS CULINARLS MEDIK.)
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The term "Protease" includes both "endopeptidases" and "exopep tidases" whereas; the term "proteinase" is used to describe only "endopeptidases" (Ryan, 1990). Majority of Pis studied in plant kingdom originate from three main families namely Leguminosae, Solanaceae and Gramineae. If a food source contains a-supply of digestible protein or carbohydrate and also an inhibitor that acts as a biological check, the larval enzymes cannot digest the food and the larva will not grow and develcp through its normal cycle Pis exhibit a very broad spectrum of activity incluJir.g suppression of pathogenic nematodes like Globodera tabaccum, G. pallid a and Meloidogyne incognita by CpTi (Williamson and Hussey, 1996), inhibition of spore germination and mycelium groWth of Alternaria allernata by buckwheat trypsin/chymotrypsin (Dunaevski et al., 1997) and cysteine protease inhibitors from pearl millet inhibit growth fo many pathogenic fungi including Trichoderma reesei (Joshi et al., 1998). Experiment of Girard et al.(1998) showed the high level of resistance of Pis con felTed by proteolytic cleavage in beetle larvae. Tobacco and peas containing multi-domain PI precursor from Nicotiana alata (Na-PI). showed increased resistance against Helicoverpa armigera (Charityet ah, 1999). There have been many examples of Pis active against certain insect species, both in in-vitro assays against insect gut protease (Pannetier ef al., 1997 ; Koiwa et al., 1998) and in in-vivo artificial diet bioassay (Urwin et al., 1997; Vain et al., 1998).
P. KUMAR、K. R. KOUNDAL
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NRC on Plant Biotechnology, Indian Agricultural Research Institute, IARI, New Delhi-110012
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