首页|Astragalus penduliflorus Lam. hairy roots: A promising biotechnological platform for obtaining anti-influenza drugs
Astragalus penduliflorus Lam. hairy roots: A promising biotechnological platform for obtaining anti-influenza drugs
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NSTL
Elsevier
? 2022 SAABThe rapid emergence of influenza virus A resistance to current antivirus drugs has raised an urgent need for developing safe and effective remedies based on plants. Hairy roots are considered an efficient system for the large-scale production of valuable compounds with potent antiviral activity. We have evaluated for the first time an antivirus activity in Astragalus penduliflorus Lam. hairy roots (APHRs) lines obtained from cotyledons (APHR 2), primary shoots (APHR 5), and hypocotyls (APHR 6) against influenza A/Aichi/2/68 (H3N2) and A/chicken/Kurgan/05/2005 (H5N1) subtypes. Estimation of the antiviral activity was based on the analysis of cytotoxic activity, selectivity and neutralization indexes. All tested samples demonstrated low toxicity for MDCK cell culture corresponding to the modern requirement for antiviral drugs. Index neutralization (IN) was 2.50 in APHR 2, 28 d extract against H3N2 subtype, whereas the highest IN against H5N1 (2.25) was detected for line APHR 5 56 d The levels of selectivity index observed in APHR 5, and APHR 6 were higher in comparison with APHR 2, corresponding to the level of antioxidants, determined by the DPPH radical scavenging assay and total antioxidant activities by amperometric method. Major phenolic compounds identified in APHRs lines extracts by HPLC analysis, including flavonoids, hydroxybenzoic, hydroxicinnamic acids, could be responsible for the antiviral activities.
NSTL
Elsevier
