首页|SARS-CoV-2 spike protein detection using slightly tapered no-core fiber-based optical transducer
SARS-CoV-2 spike protein detection using slightly tapered no-core fiber-based optical transducer
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NSTL
The label-free detection of SARS-CoV-2 spike protein is demonstrated by using slightly tapered no-core fiber(ST-NCF) functionalized with ACE2.In the fabricated sensor head,abrupt changes in the mode-field diameter at the interfaces between single-mode fiber and no-core fiber excite multi-guided modes and facilitate multi-mode interference(MMI).Its slightly tapered region causes the MMI to be more sensitive to the refractive index(RI) modulation of the surrounding medium.The transmission minimum of the MMI spectrum was selected as a sensor indicator.The sensor surface was functionalized with ACE2 bioreceptors through the pretreatment process.The ACE2-immobilized ST-NCF sensor head was exposed to the samples of SARS-CoV-2 spike protein with concentrations ranging from 1 to 10~4 ng/mL.With increasing sample concentration,we observed that the indicator dip moved towards a longer wavelength region.The observed spectral shifts are attributed to localized RI modulations at the sensor surface,which are induced by selective bioaffinity binding between ACE2 and SARS-CoV-2 spike protein.Also,we confirmed the capability of the sensor head as an effective and simple optical probe for detecting antigen protein samples by applying saliva solution used as a measurement buffer.Moreover,we compared its detection sensitivity to SARS-CoV-2 and MERS-CoV spike protein to examine its cross-reactivity.In particular,we proved the reproducibility of the bioassay protocol adopted here by employing the ST-NCF sensor head reconstructed with ACE2.Our ST-NCF transducer is expected to be beneficially utilized as a low-cost and portable biosensing platform for the rapid detection of SARS-CoV-2 spike protein.
NSTL
