首页|Monitoring of glutathione using ratiometric fluorescent sensor based on MnO2 nanosheets simultaneously tuning the fluorescence of Rhodamine 6G and thiamine hydrochloride
Monitoring of glutathione using ratiometric fluorescent sensor based on MnO2 nanosheets simultaneously tuning the fluorescence of Rhodamine 6G and thiamine hydrochloride
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NSTL
Elsevier
L-glutathione (GSH) which has reducibility and integrated detoxification plays an important role in maintaining normal immune system function. Its abnormal levels are relevant to some clinical diseases. In this work, a facile ratiometric fluorescence sensor for GSH was designed based on MnO2 nanosheets, Thiamine hydrochloride (VB1) and Rhodamine 6G (R6G). VB1 could be oxidized into fluorescent ox-VB1 due to the strong oxidizing property of MnO2, and MnO2 nanosheets simultaneously could quench the fluorescence of R6G based on the inner filter effect (IFE). MnO2 could react with GSH to form Mn2+, which caused its losing oxidizing property and quenching capacity. According to this principle, the concentration of ox-VB1 diminished, resulting in its fluorescence intensity decreasing at 455 nm and the fluorescence of R6G recovering at 560 nm. Under optimal conditions, the VB1-MnO2-R6G detection system showed a wide linear range towards GSH in the range of 1.0-300.0 mu mol L-1 with a low detection limit reaching 0.52 mu mol L-1. Furthermore, the method was also applied in the determination of GSH in human serum. (C) 2022 Elsevier B.V. All rights reserved.
NSTL
Elsevier
