首页|Highly sensitive and low-cost colourimetric detection of glucose and ascorbic acid based on silver nanozyme biosynthesized by Gleditsia australis fruit

Highly sensitive and low-cost colourimetric detection of glucose and ascorbic acid based on silver nanozyme biosynthesized by Gleditsia australis fruit

扫码查看
原文链接
  • NSTL
  • Elsevier
In this study, a simple, eco-friendly and low-cost approach was used to fabricate silver nanoparticles (AgNPs) from an aqueous extract of Gleditsia australis (GA) fruit. The nanoparticles synthesized in the optimal condition have an average size of 14 nm. The peroxidase-like activity of GA-AgNP in the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in combination with hydrogen peroxide (H2O2) was investigated. Further, optimal conditions for the use of peroxidase-like catalytic activity in sensing applications were identified. The colourimetric detection of H2O2 showed a linear range of 1-8 mM with a limit of detection (LOD) of 0.34 mM. The oxidation of TMB (red-TMB) enables the detection of glucose, which is converted into H2O2 and gluconic acid in the presence of the enzyme glucose oxidase. The observations showed linearity from 0.05 to 1.5 mM with a LOD of 0.038 mM. Moreover, the blue colour of oxidized TMB (ox-TMB) was reduced according to ascorbic acid (AA) concentration, with a linear range of 0.030.14 mM and a LOD of 3.0 mu M. The practical use of the sensing system for the detection of AA was studied using real fruit juice and showed good sensitivity. Hence, the easy-to-use peroxidase-like sensor provides a new platform for the detection of bioactive compounds in biological systems. (C) 2021 Elsevier B.V. All rights reserved.

Colourimetric detectionNanozymeHydrogen peroxideGlucoseAscorbic acidSilver nanoparticlesPEROXIDASE-LIKE ACTIVITYREDUCED GRAPHENE OXIDEHYDROGEN-PEROXIDECOLORIMETRIC DETECTIONGREEN SYNTHESISNANOPARTICLESPLANTH2O2MONTMORILLONITEPERFORMANCE

Van-Dat Doan、Van-Cuong Nguyen、Thi-Lan-Huong Nguyen、Anh-Tien Nguyen、Thanh-Danh Nguyen

展开 >

Ind Univ Ho Chi Minh City

Ho Chi Minh City Univ Educ

Vietnam Acad Sci & Technol

2022

10.1016/j.saa.2021.120709

Spectrochimica acta

Spectrochimica acta

ISSN：1386-1425
年,卷(期)：2022.268
  • 11
  • 70