首页|Peptide-based colorimetric and fluorescent dual-functional probe for sequential detection of copper(II) and cyanide ions and its application in real water samples, test strips and living cells
Peptide-based colorimetric and fluorescent dual-functional probe for sequential detection of copper(II) and cyanide ions and its application in real water samples, test strips and living cells
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NSTL
Elsevier
A novel dual-functional peptide probe FLH based on fluorescent "on-off-on " strategy and colorimetric visualization method was designed and synthesized. This new probe exhibited highly selective and rapid detection of Cu2+ with significant fluorescent "turn-off " response, with a visible colorimetric change from yellow to orange. The combination ratio of FLH to Cu2+ (1:1) was determined using ESI-HRMS spectra and Job's plot. The fluorescent emission showed a good linear response (R-2 = 0.9986) with a low detection limit of 1.5 nM. In addition, the FLH-Cu(2+ )complex displayed colorimetric changes and a fluorescent "off-on " response toward CN- over a wide pH range from 7 to 12. This detection behavior was observed within 20 s, with a limit of detection (LOD) for CN- at 12.7 nM. Based on stability and accuracy, FLH was next developed as dual-functional test strips, and was also successfully applied to detect Cu2+ and CN- in two actual water samples. More importantly, the cytotoxicity studies indicated that FLH had good biocompatibility and low toxicity, and was successfully utilized for monitoring Cu2+ and CN- in living cells through fluorescence imaging. (C) 2022 Elsevier B.V. All rights reserved.
PeptideDual-functional probeCell imagingReal water samplesTest stripsTURN-ON FLUORESCENTMETAL-IONSPARKINSONSCHEMOSENSORSALZHEIMERSDISEASESBINDINGPRIONIRON
NSTL
Elsevier
