首页|Future Clinical Application of beta-galactosidase Stabilized by Magnesium oxide Nanoparticles
Future Clinical Application of beta-galactosidase Stabilized by Magnesium oxide Nanoparticles
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NSTL
Oriental Scientific Publ Co
The present study demonstrates the application of freshly prepared neem leaf extract as a reducing agent for synthesizing magnesium oxide nanoparticles (MgO-NPs). In silico interaction of Aspergillus oryzae beta-galactosidase with MgO-NPs was observed by using molecular docking program Dock v.6.5 while the visual analyses and illustration of protein-ligand complex were investigated by utilizing chimera v.1.6.2 and PyMOL v.1.3 softwares. The prepared nanomatrix provided 83% immobilization yield, and broadened the biocatalytic activity of immobilized beta-galactosidase at higher pH and temperature ranges. Immobilized beta-galactosidase exhibited greater activity even at 5.0% galactose concentration as compared to the soluble enzyme under similar experimental conditions. Hence, the use of green nanotechnology makes the process inexpensive, and therefore, immobilization of these enzymes on such nanoparticles can help to recover the enzyme, which ultimately decreases the cost of process.
Green nanotechnologyEnzymesNeemMagnesium oxide nanoparticlesIn-silico characterization
NSTL
Oriental Scientific Publ Co
