查看更多>>摘要:Salmonella enterica serovar Indiana has become a widely prevalent non-typhoidal serovar in China in recent years. To improve the understanding of antimicrobial resistance and genetic bias in this emerging pathogen, a total of 214 S. Indiana isolates from foods, patients, and the environments in China from 2007 to 2016 were characterized for antimicrobial susceptibility, antimicrobial resistance-determining factors, and molecular typing. It was found that 208 (97.2%) exhibited resistance to ciprofloxacin, and 102 (47.7%) isolates to ceftriaxone. Ciprofloxacin resistance increased from 82.4% in 2007-2008 to 100.0% in 2015-2016. Ceftriaxone resistance increased from 29.4% in 2007-2008 to 47.1% in 2015-2016. In particular, the proportion of multidrug-resistant (MDR) S. Indiana isolates increased from 64.7% in 2007-2008 to 91.4% in 2015-2016. Mutations in the quinolone resistance determining region (QRDR) (gyrA and parC), plasmid-mediated quinolone resistance (PMQR) genes [aac(6')-Ib-cr, oqxAB, qnrA, qnrS, qnrB and qepA], extended-spectrum beta-lactamase (ESBL) genes (blaOXA, blaCTX-M, blaTEM, blaCMY and blaVEB), and class I integrons were identified in these S. Indiana isolates, along with transposon units of Delta ISEcp1-blaCTX-M-65-IS903B, ISEcp1-ISVsa5-blaCTX-M-14-IS903B, and ISEcp1-blaCTX-M-55/15-Delta orf477. To our knowledge, the ESBL gene blaVEB was identified in Salmonella for the first time. Diverse plasmid types (IncHI2, IncN, IncA/C, IncFIB, IncI1 and IncFII) were identified in these isolates, among which IncHI2-type plasmid (49.1%) was the most common type in S. Indiana. Multilocus sequence typing (MLST) showed a single ST17 in S. Indiana. Pulsed-field gel electrophoresis (PFGE) results showed that there was a genotypic diversity, and some clones might have existed in S. Indiana between patients and foods for several years. These findings suggested that the frequent emergence of S. Indiana isolates with resistance to ciprofloxacin and ceftriaxone would pose a huge threat to food safety and public health.