期刊,当代医学科学(英文) 2021年卷2期_国家学术搜索

Distinguishing Rectal Cancer from Colon Cancer Based on the Support Vector Machine Method and RNA-sequencing Data

Yan ZHANGYuan WUZi-ying GONGHai-dan YE...

368-374页

查看更多>>摘要：Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide.Several studies have indicated that rectal cancer is significantly different from colon cancer in terms of treatment,prognosis,and metastasis.Recently,the differential mRNA expression of colon cancer and rectal cancer has received a great deal of attention.The current study aimed to identify significant differences between colon cancer and rectal cancer based on RNA sequencing (RNA-seq) data via support vector machines (SVM).Here,393 CRC samples from the The Cancer Genome Atlas (TCGA) database were investigated,including 298 patients with colon cancer and 95 with rectal cancer.Following the random forest (RF) analysis of the mRNA expression data,96 genes such as HOXB13,PRAC,and BCLAF1 were identified and utilized to build the SVM classification model with the Leave-One-Out Cross-validation (LOOCV) algorithm.In the training (n=196) and the validation cohorts (n=197),the accuracy (82.1％ and 82.2 ％,respectively) and the AUC (0.87 and 0.91,respectively) indicated that the established optimal SVM classification model distinguished colon cancer from rectal cancer reasonably.However,additional experiments are required to validate the predicted gene expression levels and functions.

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NETL
NSTL
万方数据
维普

Impact of Percutaneous Transhepatic Biliary Drainage on Clinical Outcomes of Patients with Malignant Obstructive Jaundice Undergoing Laparoscopic Pancreaticoduodenectomy

He-bin WANGFeng PENGMin WANGRen-yi QIN...

375-380页

查看更多>>摘要：Preoperative biliary drainage may increase the morbidity and mortality of pancreaticoduodenectomy.Studies on percutaneous transhepatic biliary drainage (PTBD) before laparoscopic pancreaticoduodenectomy (LPD),however,are scarce.The aim of this study was to examine the impact of PTBD on clinical outcomes of patients with malignant obstructive jaundice undergoing LPD.Clinical data of 172 patients who had malignant obstructive jaundice and underwent LPD from 2014 to 2017 in our hospital were retrospectively analyzed.Demographics,catheterrelated complications,postoperative complications,and oncological outcomes were collected and analyzed.Propensity score matching was performed to minimize selection bias associated with the comparison of data between patients who underwent PTBD and then LPD (PTBD group),and those given LPD alone (LPD group).The results showed that,in the PTBD group relative to the LPD group,the operating time was significantly shortened (250.28±69.95 vs.278.58±86.51 min,P=0.0196),the intraoperative blood loss was markedly reduced (271.96±403.47 vs.429.72±482.47 mL,P=0.022),and overall rates of complications (16.33％ vs.36.49％,P=0.0025) including postoperative haemorrhage (2.04％ vs.12.16％,P=0.0072) and delayed gastric emptying (4.08％ vs.13.51％,P=0.0251) were greatly decreased.The propensity score-matched analysis,with 48 patients enrolled in each group,revealed no statistically significant differences in operating duration (262.71±68.64 vs.280.25±83.52 min,P=0.264),intraoperative blood loss (290.21±407.71 vs.373.75±422.33 mL,P=0.327) and delayed gastric emptying (4.17％ vs.12.50％,P=0.1396).PTBD group had lower incidences in overall complications (22.92％ vs.39.58％,P=0.0481) and postoperative haemorrhage (2.08％ vs.12.50％,P=0.0497) than LPD group.In conclusion,patients with malignant obstructive jaundice may benefit from PTBD procedure before LPD in terms of perioperative outcomes.

原文链接:

NETL
NSTL
万方数据
维普

Impact of Percutaneous Transhepatic Biliary Drainage on Clinical Outcomes of Patients with Malignant Obstructive Jaundice Undergoing Laparoscopic Pancreaticoduodenectomy

He-bin WANGFeng PENGMin WANGRen-yi QIN...

375-380页

查看更多>>摘要：Preoperative biliary drainage may increase the morbidity and mortality of pancreaticoduodenectomy.Studies on percutaneous transhepatic biliary drainage (PTBD) before laparoscopic pancreaticoduodenectomy (LPD),however,are scarce.The aim of this study was to examine the impact of PTBD on clinical outcomes of patients with malignant obstructive jaundice undergoing LPD.Clinical data of 172 patients who had malignant obstructive jaundice and underwent LPD from 2014 to 2017 in our hospital were retrospectively analyzed.Demographics,catheterrelated complications,postoperative complications,and oncological outcomes were collected and analyzed.Propensity score matching was performed to minimize selection bias associated with the comparison of data between patients who underwent PTBD and then LPD (PTBD group),and those given LPD alone (LPD group).The results showed that,in the PTBD group relative to the LPD group,the operating time was significantly shortened (250.28±69.95 vs.278.58±86.51 min,P=0.0196),the intraoperative blood loss was markedly reduced (271.96±403.47 vs.429.72±482.47 mL,P=0.022),and overall rates of complications (16.33％ vs.36.49％,P=0.0025) including postoperative haemorrhage (2.04％ vs.12.16％,P=0.0072) and delayed gastric emptying (4.08％ vs.13.51％,P=0.0251) were greatly decreased.The propensity score-matched analysis,with 48 patients enrolled in each group,revealed no statistically significant differences in operating duration (262.71±68.64 vs.280.25±83.52 min,P=0.264),intraoperative blood loss (290.21±407.71 vs.373.75±422.33 mL,P=0.327) and delayed gastric emptying (4.17％ vs.12.50％,P=0.1396).PTBD group had lower incidences in overall complications (22.92％ vs.39.58％,P=0.0481) and postoperative haemorrhage (2.08％ vs.12.50％,P=0.0497) than LPD group.In conclusion,patients with malignant obstructive jaundice may benefit from PTBD procedure before LPD in terms of perioperative outcomes.

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万方数据

Glabridin Relaxes Vascular Smooth Muscles by Activating BKCa Channels and Inhibiting Phosphodiesterase in Human Saphenous Vein

Cengiz GüvenAli Parlar

381-389页

查看更多>>摘要：The aim of the current study was to investigate the pharmacological activity of glabridin on the isolated human saphenous vein (SV) and explore the underlying mechanisms.Samples of patients' SVs were removed during bypass surgery,and 4-mm lengths of the vessels were placed in Krebs solution at +4℃ and hung in an isolated organ bath to assess their contraction/relaxation responses.The contraction/relaxation responses were recorded to observe if the cyclic guanosine monophosphate (cGMP)/protein kinase G (PKG) pathway mediates the relaxant effect of glabridin after treatment with blockers like ODQ (a guanylate cyclase inhibitor),KT5823 (a PKG inhibitor),isobutylmethylxanthine[IBMX,a phosphodiesterase (PDE) inhibitor],and cantharidin[Cant,a myosin light-chain phosphatase (MLCP) inhibitor].Moreover,nitric oxide (NO),cGMP,and PKG levels in SV tissues were determined by ELISA after incubation with glabridin,N(ω)-nitro-L-arginine methyl ester (L-Name,a NO synthetase inhibitor),phenylephrine (PE),ODQ,IBMX,and KT5823.The results showed that glabridin relaxed the vascular smooth muscle of human SV pretreated with PE in a dose-dependent manner,which was independent of the endothelium.The vasorelaxant effect of glabridin was only inhibited by iberiotoxin (IbTX),Cant,and KT5823.Glabridin increased cGMP and PKG levels in SV homogenates,whereas it did not alter the NO level.The enhancing effects of cGMP and PKG levels by glabridin were abolished by ODQ and KT5823.In conclusion,glabridin has a vasorelaxant effect,which is associated with the activation of BKCa channels and inhibition of PDE.

原文链接:

万方数据

Glabridin Relaxes Vascular Smooth Muscles by Activating BKCa Channels and Inhibiting Phosphodiesterase in Human Saphenous Vein

Cengiz GüvenAli Parlar

381-389页

查看更多>>摘要：The aim of the current study was to investigate the pharmacological activity of glabridin on the isolated human saphenous vein (SV) and explore the underlying mechanisms.Samples of patients' SVs were removed during bypass surgery,and 4-mm lengths of the vessels were placed in Krebs solution at +4℃ and hung in an isolated organ bath to assess their contraction/relaxation responses.The contraction/relaxation responses were recorded to observe if the cyclic guanosine monophosphate (cGMP)/protein kinase G (PKG) pathway mediates the relaxant effect of glabridin after treatment with blockers like ODQ (a guanylate cyclase inhibitor),KT5823 (a PKG inhibitor),isobutylmethylxanthine[IBMX,a phosphodiesterase (PDE) inhibitor],and cantharidin[Cant,a myosin light-chain phosphatase (MLCP) inhibitor].Moreover,nitric oxide (NO),cGMP,and PKG levels in SV tissues were determined by ELISA after incubation with glabridin,N(ω)-nitro-L-arginine methyl ester (L-Name,a NO synthetase inhibitor),phenylephrine (PE),ODQ,IBMX,and KT5823.The results showed that glabridin relaxed the vascular smooth muscle of human SV pretreated with PE in a dose-dependent manner,which was independent of the endothelium.The vasorelaxant effect of glabridin was only inhibited by iberiotoxin (IbTX),Cant,and KT5823.Glabridin increased cGMP and PKG levels in SV homogenates,whereas it did not alter the NO level.The enhancing effects of cGMP and PKG levels by glabridin were abolished by ODQ and KT5823.In conclusion,glabridin has a vasorelaxant effect,which is associated with the activation of BKCa channels and inhibition of PDE.

原文链接:

NETL
NSTL
万方数据

IgG4-Related Disease: A Retrospective Chinese Study of Features and Treatment Response of 98 Patients Including 4 Rare Cases

Yue-ying PANShu-chang ZHOUYu-jin WANGTing-ting ZHU...

390-397页

查看更多>>摘要：The features and treatment of 98 Chinese patients with immunoglobulin G4 (IgG4)-related disease (IgG4-RD) referred to a single tertiary referring centre were reviewed.Patients diagnosed with IgG4-RD according to the comprehensive diagnostic criteria (CDC) were included in the retrospective study from May 2012 to March 2019.We collected data on clinical,laboratory,imaging,histological features and treatment.Totally,98 patients with IgG4-RD were enrolled.The common clinical manifestations included abdominal pain,salivary gland swelling and lymphadenopathy.51％ of the patients had multiple organs involvement.Lymph nodes,pancreas and salivary glands were most commonly involved.Four rare sites including ulna,cerebellum,scalp,and mammary gland were found.The serum IgG4 level was increased by 85.7％.The serum IgG4 level was positively correlated with the number of involved organs,IgG and IgG4/IgG.Low C3 and C4 levels were observed in 37.5％ and 12.2％ patients respectively,and all patients with kidney involvement had hypocomplementemia.A total of 54 patients underwent tissue biopsies,and 55.6％,31.5％ and 11.1％ cases were diagnosed as definite,probable and possible IgG4-RD,respectively.Eighty-eight patients received glucocorticoids (GCs) therapy.Five patients underwent radical surgery to remove the lesion.73％ of them presented a complete or partial remission.IgG4-RD is a systemic fibroinflammatory disease with involvement of multiple organs throughout the body including some rare sites.Most IgG4-RD patients had increased serum IgG4 levels and patients with kidney involvement showed hypocomplementemia.GCs therapy is effective.More research is needed to provide a more reliable basis for the diagnosis and treatment of patients.

原文链接:

NETL
NSTL
万方数据

IgG4-Related Disease: A Retrospective Chinese Study of Features and Treatment Response of 98 Patients Including 4 Rare Cases

Yue-ying PANShu-chang ZHOUYu-jin WANGTing-ting ZHU...

390-397页

查看更多>>摘要：The features and treatment of 98 Chinese patients with immunoglobulin G4 (IgG4)-related disease (IgG4-RD) referred to a single tertiary referring centre were reviewed.Patients diagnosed with IgG4-RD according to the comprehensive diagnostic criteria (CDC) were included in the retrospective study from May 2012 to March 2019.We collected data on clinical,laboratory,imaging,histological features and treatment.Totally,98 patients with IgG4-RD were enrolled.The common clinical manifestations included abdominal pain,salivary gland swelling and lymphadenopathy.51％ of the patients had multiple organs involvement.Lymph nodes,pancreas and salivary glands were most commonly involved.Four rare sites including ulna,cerebellum,scalp,and mammary gland were found.The serum IgG4 level was increased by 85.7％.The serum IgG4 level was positively correlated with the number of involved organs,IgG and IgG4/IgG.Low C3 and C4 levels were observed in 37.5％ and 12.2％ patients respectively,and all patients with kidney involvement had hypocomplementemia.A total of 54 patients underwent tissue biopsies,and 55.6％,31.5％ and 11.1％ cases were diagnosed as definite,probable and possible IgG4-RD,respectively.Eighty-eight patients received glucocorticoids (GCs) therapy.Five patients underwent radical surgery to remove the lesion.73％ of them presented a complete or partial remission.IgG4-RD is a systemic fibroinflammatory disease with involvement of multiple organs throughout the body including some rare sites.Most IgG4-RD patients had increased serum IgG4 levels and patients with kidney involvement showed hypocomplementemia.GCs therapy is effective.More research is needed to provide a more reliable basis for the diagnosis and treatment of patients.

原文链接:

万方数据

Fully Automatic Scar Segmentation for Late Gadolinium Enhancement MRI Images in Left Ventricle with Myocardial Infarction

Zheng-hong WULi-ping SUNYun-long LIUDian-dian DONG...

398-404页

查看更多>>摘要：Numerous methods have been published to segment the infarct tissue in the left ventricle,most of them either need manual work,post-processing,or suffer from poor reproducibility.We proposed an automatic segmentation method for segmenting the infarct tissue in left ventricle with myocardial infarction.Cardiac images of a total of 60 diseased hearts (55 human hearts and 5 porcine hearts) were used in this study.The epicardial and endocardial boundaries of the ventricles in every 2D slice of the cardiac magnetic resonance with late gadolinium enhancement images were manually segmented.The subsequent pipeline of infarct tissue segmentation is fully automatic.The segmentation results with the automatic algorithm proposed in this paper were compared to the consensus ground truth.The median of Dice overlap between our automatic method and the consensus ground truth is 0.79.We also compared the automatic method with the consensus ground truth using different image sources from different centers with different scan parameters and different scan machines.The results showed that the Dice overlap with the public dataset was 0.83,and the overall Dice overlap was 0.79.The results show that our method is robust with respect to different MRI image sources,which were scanned by different centers with different image collection parameters.The segmentation accuracy we obtained is comparable to or better than that of the conventional semi-automatic methods.Our segmentation method may be useful for processing large amount of dataset in clinic.

原文链接:

万方数据

Fully Automatic Scar Segmentation for Late Gadolinium Enhancement MRI Images in Left Ventricle with Myocardial Infarction

Zheng-hong WULi-ping SUNYun-long LIUDian-dian DONG...

398-404页

查看更多>>摘要：Numerous methods have been published to segment the infarct tissue in the left ventricle,most of them either need manual work,post-processing,or suffer from poor reproducibility.We proposed an automatic segmentation method for segmenting the infarct tissue in left ventricle with myocardial infarction.Cardiac images of a total of 60 diseased hearts (55 human hearts and 5 porcine hearts) were used in this study.The epicardial and endocardial boundaries of the ventricles in every 2D slice of the cardiac magnetic resonance with late gadolinium enhancement images were manually segmented.The subsequent pipeline of infarct tissue segmentation is fully automatic.The segmentation results with the automatic algorithm proposed in this paper were compared to the consensus ground truth.The median of Dice overlap between our automatic method and the consensus ground truth is 0.79.We also compared the automatic method with the consensus ground truth using different image sources from different centers with different scan parameters and different scan machines.The results showed that the Dice overlap with the public dataset was 0.83,and the overall Dice overlap was 0.79.The results show that our method is robust with respect to different MRI image sources,which were scanned by different centers with different image collection parameters.The segmentation accuracy we obtained is comparable to or better than that of the conventional semi-automatic methods.Our segmentation method may be useful for processing large amount of dataset in clinic.

原文链接:

NETL
NSTL
万方数据
维普