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国际中医中药杂志
中国中医研究院中医药信息研究所
国际中医中药杂志

中国中医研究院中医药信息研究所

曹洪欣

月刊

1673-4246

guowaiyixue@yahoo.com.cn

010-64014411-3225

100700

北京市东直门内南小街16号中国中医科学院中医药信息研究所

国际中医中药杂志/Journal International Journal of Traditional Chinese Medicine北大核心CSTPCD
查看更多>>中华人民共和国卫生部主管，中华医学会、中国中医科学院中医药信息研究所主办。本刊以反映国内外中医药学研究的最新进展，促进国内外中医药学的学术交流为己任，以中医药科研、临床、生产、管理工作者为主要对象，介绍中医药学领域有关的新进展、新技术和新方法。开设了专题论坛、特别报道、临床研究、实验研究、临床调研、思路与方法、研究动态、信息研究、临床报道、临证心得、综述等栏目。为中国科技论文统计源期刊（中国科技核心期刊）。
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    基于石氏醒脑开窍理论探讨针灸脑病的辨证论治

    秦文秀许军峰高莹王坪霏...
    137-141页
    查看更多>>摘要：通过挖掘醒脑开窍理论的核心思想，分析醒脑开窍理论与中医脑病的关系，探究针灸辨治中医脑病的必要性，以明晰针灸脑病当如何辨证论治。“醒脑开窍”是调神理论的核心，是立足于醒脑、醒神、调神的原则而确立，其调神思想与中医脑病证治密不可分。基于针灸疗法的特色及中医脑病的复杂性和特殊性，针灸治疗脑病理应有其独特的辨证论治体系。针灸实践过程中，中医脑病证治当以“脑”为本，以“醒神调神”为总治则，在“醒脑开窍针法”主穴基础上化裁，针对不同脑病配合相应的治法。 By exploring the core ideas of the "Xingnao Kaiqiao" theory, analyzing the relationship between the "Xingnao Kaiqiao" theory and the TCM encephalopathy, and exploring the necessity of acupuncture for the treatment of TCM encephalopathy, in order to clarify how to diagnose and treat brain diseases in acupuncture and moxibustion. The "Xingnao Kaiqiao" is the core of the theory of the "Tiaoshen", the acupuncture of "Xingnao Kaiqiao" was based on the principle of "Xingnao","Xingshen" and "Tiaoshen", the idea of the "Tiaoshen" was inseparable from the diagnosis and treatment of the TCM encephalopathy. Based on the special features of acupuncture therapy and complexity and particularity of TCM encephalopathy, acupuncture and moxibustion treatment of encephalopathy was supposed to have their own unique system of syndrome differentiation and treatment. During the practice of acupuncture, the diagnosis and treatment of TCM encephalopathy should be based on "brain", "Xingnao Tiaoshen" as a general rule, adding and subtracting from the main points of the "Xingnao Kaiqiao", and the different brain diseases are matched with appropriate treatments.

    醒脑开窍针灸疗法脑病辨证论治

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    基于气血升降理论探讨心肌梗死后抑郁的中医治法

    王璐绮谢连娣吴正波樊红雨...
    142-146页
    查看更多>>摘要：心肌梗死后抑郁的病机与气血升降理论密切相关，其核心病机为气血失常，心神受损，升降失司，枢轴不运。治当气血升降同调，治以益气活血、调达升降，其中益气活血以益气为先，根据血瘀程度适当选用活血药，使血脉通利，神有所依；调达升降以调节肝升肺降与脾胃为关键，重视药物升降浮沉之性，顺应脏腑的生理功能，恢复脏腑气机升降运动。 Depression after myocardial infarction is closely related to the theory of ascending and descending of qi and blood. The core pathogenesis is analyzed as disorder of qi and blood, mental damage, uncontrolled upward and downward movement, and pivot movement failure. The treatment method is to regulate qi and blood, invigorate qi and activate blood circulation and restore the rise and fall of visceral qi. Nourishing qi and promoting blood circulation should invigorate qi in the first. Appropriate blood activating drugs should be selected according to the degree of blood stasis, so that the blood circulation is smooth and the spirit has dependence. The key to restoring the normal balance of qi movement of visceral organs is to regulate not only liver ascending and lung descending but also spleen. Clinicians need pay attention to the nature of drug lifting and falling, conforming the physiological functions of the organs, to restore the rising and falling of the qi movement of internal organs.

    心肌梗塞抑郁症气血升降肝升肺降

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    从“气脉常通”理论探讨益气活血法治疗糖尿病周围神经病变

    杨宏丽李春晖郭馨蔓袁名熠...
    147-150页
    查看更多>>摘要：糖尿病周围神经病变（DPN）为糖尿病常见慢性并发症，可导致足部溃疡、坏疽，严重者可导致截肢，影响患者生活质量。DPN属中医“痹病”“痿病”等范畴，主要病机为气血阴阳亏虚，痰浊瘀血痹阻脉络，临床以气虚血瘀证多见。本文基于《黄帝内经》“气脉常通”理论，提出DPN气虚血瘀证患者的治疗应以“益气活血、化瘀通痹”为原则，使患者气脉通达，延缓疾病进展，为DPN的中医治疗提供参考。 Diabetic peripheral neuropathy (DPN) is the common chronic complication of diabetes, which can lead to foot ulcers, gangrene, and amputation in severe cases, seriously affecting their quality of life. DPN belongs to the category of "arthralgia", "hemorrhoids" and other categories of TCM, and the main pathogenesis is the deficiency of qi and blood, yin and yang, and the obstruction of the meridians by phlegm and stasis. Clinically, DPN is more common with qi deficiency and blood stasis syndrome. Based on the theory of "qi meridian constant communication" in the Huang Di Nei Jing, this article proposed that for patients with DPN with qi deficiency and blood stasis syndrome, the treatment should be based on the principle of "invigorating qi and activating blood circulation, dissolving stasis and arthralgia", so that the patients' qi meridian can be accessible, delay the disease progression, and provide reference for the TCM treatment of DPN.

    糖尿病神经病变气脉常通气虚血瘀益气活血

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    从“乙癸同源”探讨雷公藤生殖毒性的中医药减毒思路

    李子颢王可郭柏村李晟尧...
    151-155页
    查看更多>>摘要：雷公藤广泛应用于自身免疫系统疾病的治疗，但其明显的生殖毒性使其临床应用及推广受到限制。目前针对雷公藤的生殖损伤，尚未有明确的解决方案。中医认为，雷公藤的生殖毒性当责之于其辛苦寒凉之性，久用可燥肝血、伤肝阳、耗肾精、损肾阳，破坏脏腑的气血阴阳平衡，致不孕不育。本文根据肝肾与人体生殖功能的联系，提出从“乙癸同源”角度认识雷公藤的生殖毒性，并依据“肝肾同治”思想探索雷公藤生殖毒性的减毒之法，以期为临床安全应用拓展中医理论思考。 Tripterygium wilfordii is widely used in the treatment of autoimmune system diseases, but its obvious reproductive toxicity limits the clinical application and promotion of the drug. At present, there is no clear solution to the reproductive injury of Tripterygium wilfordii. TCM believes that its reproductive toxicity lies in its properties of pungent, bitter, and cold. Long-term use can dry liver and blood, hurt liver and yang, consume kidney essence, damage kidney and yang, destroy the balance of qi and blood, yin and yang in the internal organs, and cause infertility. Based on the relationship between liver and kidney and human reproductive function, this article proposed to understand the reproductive toxicity of Tripterygium wilfordii from the perspective of "Yi and Gui homology", and explored the method of reducing the reproductive toxicity of Tripterygium wilfordii according to the thought of "treating liver and kidney together", in order to expand the theoretical thinking of TCM for the safe clinical application of this drug.

    雷公藤乙癸同源肝肾同源生殖毒性

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    以上海中医药大学附属岳阳中西医结合医院为例探析中医医院医疗质量评价指标

    施扬王景潇杭斐赵震宇...
    156-161页
    查看更多>>摘要：分析中医医院医疗质量的评价指标，实现对中医医院医疗质量进行客观公正、精准的评价。通过分析中医医院医疗质量相关文献和专题小组讨论，总结出21个三级中医医院医疗质量评价指标。采用专家咨询法进行问卷调查，问卷调查40名专家，有效回收37份，积极系数为92.50%；经2轮专家咨询，确定评价指标为：门诊中医中药饮片使用强度（中药饮片使用率、中药饮片处方数、中药饮片剂量、中药饮片服务价格）、中医医疗技术使用强度（中医技术比例、中医技术项目数、中医技术费用、中医技术疗程）指标。利用上海中医药大学附属岳阳中西医结合医院运营决策支持系统（BI）平台对10个科室相关数据进行案例分析，发现10个科室数据分析结果与医院实际医疗质量情况基本一致。表明确定的中医医院医疗质量评价指标可反映实际医疗质量情况，具有一定的科学性、可行性和适用性，为完善中医医院医疗质量评价指标提供参考。 To improve the evaluation indicators of medical quality in TCM hospitals To realize objective, fair, and accurate evaluation of the quality of TCM. Based on relevant literature on medical quality in traditional Chinese medicine hospitals research and thematic group discussions, 21 evaluation indicators for TCM characteristics were formed. A questionnaire survey was conducted among 40 experts, and 37 were effectively collected, with a positive coefficient of 92.50%. After two rounds of expert consultation, the evaluation indicators were determined to be: the intensity of outpatient use of TCM decoction pieces (utilization rate of TCM decoction pieces, prescription number of TCM decoction pieces, dosage of TCM decoction pieces, and service price of TCM decoction pieces), the intensity of the use of TCM technology (proportion of TCM technology, number of TCM projects, cost of TCM technology, and course of treatment). Case studies were conducted on relevant data from 10 departments using the operational decision support system (BI) platform of Yueyang Integrated Traditional Chinese and Western Medicine Hospital affiliated with Shanghai University of Traditional Chinese Medicine to verify the rationality of indicators. The 10 departments were analyzed and evaluated, and the results obtained were basically consistent with the actual medical quality situation of the hospital. The indicators used in this study can reflect the actual medical quality situation, and have a certain degree of scientificity, feasibility, and applicability, providing reference for improving the medical quality evaluation indicators of TCM hospitals.

    中医医院医疗质量评价指标运营决策支持系统平台

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    植物药在北美和欧盟的注册现状

    谭勇温博魏嘉茵冯小玉...
    162-167页
    查看更多>>摘要：以植物药身份进行中药国际注册是实现中药国际化的必要前提。北美和欧盟在世界中草药市场占据重要地位，且药品管理法规和质量监管要求较为完备并具有先进性。本文通过总结北美和欧盟对植物药的定义，梳理和解读相关法律法规体系和政策文件，构建植物药的注册路径，基于当前正在申请和已批准的植物药在海外市场成功上市的实例，探讨北美和欧盟植物药市场注册现状，认为今后应构建与国际匹配的中医药质量标准证据体系，开展符合国际标准的中药产品临床试验，制定相同或高于国际主流药品生产质量管理规范的标准，探索中药复方的注册路径，招募专业人员组建国际化注册申报团队，为我国中药产品海外注册提供策略和实践支撑，促进中药产品进入世界主流市场，实现中医药国际化的高质量发展。 International registration of herbal drugs is a necessary prerequisite for the internationalization of Chinese materia medica. North America and European Union occupy an important position in the world herbal drugs market, and their drug administration and quality supervision requirements are relatively complete and progressiveness. By summarizing the definition of herbal drugs in North America and European Union, combing and interpreting the relevant laws, regulations and policy documents, this article constructed the registration path of herbal drugs, and discussed the current status of market registration of herbal drugs in North America and European Union based on the examples of successful marketing of herbal drugs under current application and approval in overseas markets. This article believed that in the future, an internationally matched TCM quality standard evidence system should be built, clinical trials of TCM products in line with international standards should be carried out, standards that are the same or higher than international mainstream GMP should be developed, the registration path of TCM compounds should be explored, and professionals to establish an international registration application team should be recruited to provide theoretical and practical support for China's overseas registration of Chinese TCM products, promote TCM products to enter the world mainstream market, and achieve high-quality development of TCM internationalization.

    植物药国际注册北美欧洲联盟

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    齐氏通管方对输卵管性不孕介入复通术后妊娠结局影响的真实世界研究

    高晓晴章晓乐胡攀伟闫晓彤...
    168-174页
    查看更多>>摘要：目的 评价齐氏通管方对输卵管性不孕（TFI）患者介入复通术后妊娠结局的影响。 方法 本研究为基于真实世界和倾向性评分匹配的回顾性研究。选取2020年1月－2021年10月上海中医药大学附属曙光医院和浦东新区妇幼保健院TFI患者260例作为观察对象，按介入复通术后是否联用齐氏通管方分为中药联合组123例和对照组137例，将可能影响TFI患者介入复通术后妊娠结局的混杂因素作为协变量，采用倾向性评分匹配（PSM）得到组间协变量均衡的新样本，比较2组患者随访12个月内的宫内妊娠率、异位妊娠率、生化妊娠率、早期流产率及不良反应，评价TFI介入复通术后联合齐氏通管方对宫内妊娠率的影响。 结果 年龄、不孕年限、不孕类型、流产史、异位妊娠史、生化妊娠史、宫腔手术史、盆腹腔手术史、输卵管通畅程度对TFI患者介入复通术后是否宫内妊娠有影响（P<0.05），其中年龄［OR（95%CI）为0.843（0.769，0.926）］、盆腹腔手术史［OR（95%CI）为0.477（0.248，0.920）］、输卵管阻塞程度［OR（95%CI）为0.152（0.046，0.500）］为其独立影响因素（P<0.01或P<0.05）。PSM后2组各有81例患者，其中复通术后9、12个月中药联合组宫内妊娠率分别为48.1%（39/81）、58.0%（47/81），对照组分别为32.1%（26/81）、35.8%（29/81），2组比较差异有统计学意义（χ2值分别为4.34、8.03，P<0.01）；2组异位妊娠率、生化妊娠率、早期流产率比较，差异无统计学意义（P>0.05）；2组治疗过程中均无明显不良反应。 结论 齐氏通管方联合介入复通术可有效提高TFI患者的宫内妊娠率，缩短妊娠期待时间，且安全性较高。 Objective To evaluate the effect of Qishi Tongguan Prescription on pregnancy outcomes after interventional recanalisation in patients with tubal infertility (TFI). Methods This was a retrospective study based on real-world and propensity score matching. Totally 260 patients with TFI from January 2020 to October 2021 in Shuguang Hospital of Shanghai University of Traditional Chinese Medicine and Maternal and Child Health Hospital of Pudong New Area were selected as observation subjects, and were divided into 123 cases in the TCM combination group and 137 cases in the control group based on whether they were treated with Qishi Tongguan Prescription in combination with interventional revascularization. Propensity score matching (PSM) was used as a covariate to obtain a new sample of inter group covariate equilibrium, and confounding factors that may affect the pregnancy outcome of TFI patients undergoing interventional recanalization surgery were used as covariates. The intrauterine pregnancy rate, ectopic pregnancy rate, biochemical pregnancy rate, early abortion rate and adverse reactions of the two groups of patients within 12 months of follow-up were compared, and the influence of TFI intervention and recanalization combined with Qishi Tongguan Prescription on intrauterine pregnancy rate was evaluated. Results Age, years of infertility, type of infertility, history of miscarriage, history of ectopic pregnancy, history of biochemical pregnancy, history of uterine surgery, history of pelvic laparotomy, and degree of tubal patency had an effect on whether intrauterine pregnancy was achieved after interventional reversal in patients with TFI (P<0.05), with age [OR (95%CI) was 0.843 (0.769, 0.926)], history of pelvic laparotomy [OR (95%CI) was 0.477 (0.248, 0.920)] and the degree of tubal obstruction [OR (95%CI) was 0.152 (0.046, 0.500)] were independent factors (P<0.01 orP<0.05). 81 patients were seen in each of the 2 groups after PSM, of whom the intrauterine pregnancy rates in the combined herbal group at 9 and 12 months after recanalisation were 48.1% (39/81) and 58.0% (47/81) respectively, compared with 32.1% (26/81) and 35.8% (29/81) in the control group, with statistical significance between the 2 groups (χ2 values of 4.34 and 8.03, respectively, P<0.01) there was no statistical significance in the ectopic pregnancy rate, biochemical pregnancy rate and early abortion rate between the 2 groups (P>0.05). There were no significant adverse reactions during the treatment. Conclusion Qishi Tongguan Prescription combined with interventional recanalization can effectively improve the intrauterine pregnancy rate and shorten the waiting time for pregnancy in patients with TFI with higher safety.

    不育,女性输卵管阻塞齐氏通管方介入复通术后妊娠结局真实世界研究

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    通督调神针刺法对缺血性卒中后轻度认知功能障碍患者血管内皮功能及炎症因子水平的影响

    刘辉李佩芳孙培养吴杰...
    175-180页
    查看更多>>摘要：目的 探讨通督调神针刺法对缺血性卒中（IS）后轻度认知功能障碍（MCI）患者血管内皮功能及炎症因子水平的影响。 方法 回顾性分析2020年1月－2022年9月本院71例IS后MCI患者的临床资料，按治疗方法分为通督调神针刺组31例与常规体针组40例。2组均口服尼莫地平片，在此基础上常规体针组给予常规体针治疗，通督调神针刺组给予通督调神针刺法治疗。2组均14 d为1个疗程，共治疗2个疗程。分别于治疗前后采用放射免疫法检测血清NO、内皮素-1（ET-1）水平，ELISA法检测血清碱性成纤维细胞生长因子（bFGF）、VEGF水平及血浆同型半胱氨酸（Hcy）、IL-6水平；采用蒙特利尔认知评估量表（MoCA量表）、简易智力状态检查量表（MMSE）评估患者认知功能及智力水平，评价临床疗效。 结果 通督调神针刺组总有效率为90.32%（28/31）、常规体针组为70.00%（28/40），2组比较差异有统计学意义（χ2=4.33，P=0.037）。通督调神针刺组治疗后血浆Hcy、IL-6水平低于常规体针组（t值分别为2.57、9.36，P<0.05或P<0.01）；血清bFGF、VEGF、NO水平高于常规体针组（t值分别为10.03、9.29、8.17，P<0.01），ET-1水平低于常规体针组（t=2.41，P=0.019）；通督调神针刺组治疗后MoCA量表评分［（28.24±4.45）分比（25.32±4.34）分，t=2.78］、MMSE量表评分［（28.73±1.44）分比（28.02±1.22）分，t=2.25］高于常规体针组（P<0.01）。 结论 通督调神针刺法有利于改善IS后MCI患者的血管内皮功能，降低炎症因子水平，促进认知功能及智力水平的恢复，提高临床疗效。 Objective To explore the effects of Tongdu Tiaoshen acupuncture on vascular endothelial function and inflammatory factors in patients with mild cognitive impairment (MCI) after ischemic stroke (IS). Methods A retrospective analysis was performed on the clinical data of 71 patients with MCI after IS in the hospital between January 2020 and September 2022. According to different treatment methods, they were divided into Tongdu Tiaoshen acupuncture group (n=31, Tongdu Tiaoshen acupuncture + oral nimodipine tables) and routine body-acupuncture group (n=40, routine body-acupuncture group + oral nimodipine tables). Both groups were treated for 2 courses (14 d/course). Before and after treatment, levels of serum NO and endothelin-1 (ET-1) were detected by radioimmunoassay, and levels of serum basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF), plasma homocysteine (Hcy) and IL-6 were detected by ELISA. The cognitive function of patients was evaluated and intelligence level by Montreal Cognitive Assessment Scale (MoCA), Mini-Mental State Examination(MMSE) and the clinical curative effect was also evaluated. Results The total response rates in Tongdu Tiaoshen acupuncture group and routine body-acupuncture group were 90.32% (28/31) and 70.00% (28/40), and the difference between the two groups was statistically significant (χ2=4.33, P=0.037). After treatment, levels of plasma Hcy and IL-6 in Tongdu Tiaoshen acupuncture group were lower than those in the routine body-acupuncture group (t=2.57, 9.36, P<0.05 orP<0.01). After treatment, levels of serum bFGF, VEGF and NO in Tongdu Tiaoshen acupuncture group were significantly higher than those in the routine body-acupuncture group (t=10.03, 9.29, 8.17, P<0.01), while ET-1 level was significantly lower than that of the routine body-acupuncture group (t=2.41, P=0.019). After treatment, MoCA score [(28.24±4.45) vs. (25.32±4.34), t=2.78], MMSE score [(28.73±1.44) vs. (28.02±1.22),t=2.25] in Tongdu Tiaoshen acupuncture group were higher than those in the routine body-acupuncture group (P<0.01). Conclusion Tongdu Tiaoshen acupuncture is beneficial to improve vascular endothelial function, reduce levels of inflammatory factors, promote the recovery of cognitive function and improve curative effect in patients with MCI after IS.

    缺血性卒中认知功能障碍碱性成纤维细胞生长因子血管内皮生长因子炎症因子

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    逍生散超声雾化联合睑板腺按摩治疗睑板腺功能障碍型干眼肝郁阴虚证临床研究

    张志芳杨安谢立科匡薪錡...
    181-185页
    查看更多>>摘要：目的 评价逍生散超声雾化联合睑板腺按摩治疗睑板腺功能障碍型干眼肝郁阴虚证的临床疗效。 方法 随机对照试验研究。选取2021年3－9月中国中医科学院眼科医院睑板腺功能障碍型干眼肝郁阴虚证患者50例（100只眼）作为观察对象，按随机数字表法分为2组，每组25例（50只眼）。对照组予0.1%玻璃酸钠滴眼液联合睑板腺按摩，治疗组予逍生散超声雾化联合睑板腺按摩。2组均治疗4周。分别于治疗前后观察患者泪膜破裂时间（BUT），行泪液分泌试验（SⅠT）并进行角结膜荧光染色（FL）评分、中医症状评分、睑板腺功能评分，评价临床疗效。 结果 治疗组治疗后BUT较对照组延长（t=8.76，P<0.01）、SⅠT较对照组升高（t=6.18，P<0.01），FL评分、中医症状积分、睑板腺功能评分较对照组降低（t值分别为2.19、5.36、12.09，P<0.01）。治疗组总有效率为96.0%（24/25）、对照组为68.0%（17/25），2组比较差异有统计学意义（χ2=4.88，P=0.027）。 结论 逍生散超声雾化联合睑板腺按摩可有效改善睑板腺功能障碍型干眼肝郁阴虚证患者的临床症状，且安全有效。 Objective To observe the clinical efficacy of Xiaosheng Powder ultrasonic nebulization combined with meibomian gland massage in treating liver depression and Yin deficiency pattern of meibomian gland dysfunction dry eye. Methods Randomized controlled trial. From March 2021 to September 2021, a total of 50 patients (100 eyes) diagnosed with meibomian gland dysfunction dry eye of the liver depression and Yin deficiency pattern at the Ophthalmology Hospital of China Academy of Chinese Medical Sciences were selected. They were randomly divided into two groups using a random number table, with 25 patients (50 eyes) in each group. The control group received 0.1% sodium hyaluronate eye drops combined with meibomian gland massage, while the treatment group received Xiaosheng Powder nebulization combined with meibomian gland massage. Both groups were treated for 4 weeks. Before and after treatment, the tear film break-up time (BUT), Schirmer I test (SIT), corneal fluorescein staining (FL) scores, TCM symptom scores, and meibomian gland function scores were observed and evaluated. Adverse reactions were recorded. Results After treatment, the treatment group exhibited statistically significant improvements compared to the control group in the following aspects: BUT was significantly longer in the treatment group (t=8.76, P<0.01) SIT values were significantly higher in the treatment group (t=6.18, P<0.01) FL scores, TCM symptom scores and meibomian gland function scores were significantly lower than in the treatment group (t values were 2.19, 5.36 and 12.09, P<0.01). The total effective rate in the treatment group was 96.0% (24/25), while in the control group, it was 68.0% (17/25), and the difference between the two groups was statistically significant (χ2=4.88, P=0.027). Conclusion The combination of Xiaosheng Powder ultrasonic nebulization and meibomian gland massage is effective in improving the clinical symptoms of patients with meibomian gland dysfunction dry eye of the liver depression and Yin deficiency pattern. Furthermore, this approach is considered safe and efficient.

    干眼病睑板腺功能障碍逍生散雾化睑板腺按摩肝郁阴虚

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    健脾补肾解毒方调控JAK2/STAT3通路对肝癌细胞增殖与迁移的影响

    李会娣冯媛媛赖优莹贾茹...
    186-190页
    查看更多>>摘要：目的 研究健脾补肾解毒方对肝癌细胞增殖、迁移的影响，探讨其作用机制。 方法 以人高转移肝癌细胞株（HCCLM3）为研究对象，将其按随机数字表法分为对照组、中药组（100、200、400、800、1 600、3 200 μg/ml健脾补肾解毒方）及西药组（2.5、5、10、20、40 μmol/L索拉非尼），采用胞计数试剂（CCK-8）法检测细胞活力；将HCCLM3细胞按随机数字表法分为中药低、中、高剂量组（健脾补肾解毒方醇提液100、800、1 600 μg/ml）、西药组（索拉非尼20 μmol/L）及对照组，采用划痕实验检测HCCLM3迁移情况。将HCCLM3细胞按随机数字表法分为对照组、中药组（健脾补肾解毒方800 μg/ml）和联合组（健脾补肾解毒方800 μg/ml+索拉非尼20 μmol/L），采用Western blot法检测各组细胞蛋白激酶/信号传导子和转录激活子（JAK2/STAT3）通路相关蛋白（p-JAK2、JAK2、p-STAT3、STAT3）表达情况。 结果 与对照组比较，中药组及西药组HCCLM细胞活力及迁移率降低（P<0.01或P<0.05）；与对照组比较，中药组、联合组P-JAK2、JAK2、p-STAT3、STAT3蛋白表达降低（P<0.05），且联合组JAK2蛋白表达低于中药组（P<0.05）。 结论 健脾补肾解毒方通过调控JAK2/STAT3通路对肝癌细胞的增殖与迁移产生抑制作用。 Objective To explore the effects of Jianpi Bushen Jiedu Prescription on the proliferation and migration of hepatocellular carcinoma cells To discuss its possible mechanism. Methods Using human highly metastatic liver cancer cell line (HCCLM3) as the research object, they were randomly divided into control group and TCM group (100, 200, 400, 800, 1 600, 3 200 μg/ml Jianpi Bushen Jiedu Prescription) and Western medicine group (2.5, 5, 10, 20, 40 μmol/L sorafenib) using a random number table method. Cell viability was detected using cell counting reagent (CCK-8) method HCCLM3 cells were divided into control group and TCM (Jianpi Bushen Jiedu Prescription 800 μg/ml) group and combined group (Jianpi Bushen Jiedu Prescription 800 μg/ml +sorafenib 20 μmol/L). Western blot method was used to detect the protein expressions of kinase/signaling transducer and transcriptional activator (JAK2/STAT3) pathway related proteins (p-JAK2, JAK2, p-STAT3, STAT3) in each group. Results Compared with the control group, viability and mobility of HCCLM cell in TCM group and Western medicine group decreased (P<0.01 orP<0.05) compared with the control group, the protein expressions of P-JAK2, JAK2, P-STAT3 and STAT3 in the TCM group and the combined group decreased (P<0.05), and the JAK2 protein expression in the combined group was lower than that in the TCM group (P<0.05). Conclusion Jianpi Bushen Jiedu Prescription can inhibit the proliferation and migration of HCC cells by regulating JAK2/STAT3 pathway.

    肝肿瘤健脾补肾解毒方JAK2/STAT3通路细胞增殖细胞迁移

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