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海南医科大学学报（英文版）
海南医科大学学报（英文版）

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海南医科大学学报（英文版）/Journal Journal of Hainan Medical University
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    The latest research progress of novel coronavirus "Omicron sub‑variant BA.5"

    WANG Cai?hongYAO Xiao?wenWANG RongZHOU Yu?xia...
    1-5页
    查看更多>>摘要：Since the outbreak of COVID‑19, severe acute respiratory syndrome coronavirus 2 genome is still mutating, forming a variety of variants with strong transmission capacity, causing the spread of the epidemic worldwide, posing a serious threat to people's physical and mental health, and posing a major challenge to global public health. Omicron remains the main variant in several outbreaks worldwide, accounting for about 99％ of the global genetic sequence. Recently, the World Health Organization announced that the subvariant of Omicron BA.5 has been found in more than 100 countries and regions around the world, causing the global epidemic rebound. However, there are few studies on the subvariant BA.5. This article reviews the latest research progress in epidemiology, infectivity, pathogenicity, vaccine and monoclonal antibody protection against Omicron subvariant BA.5, in order to provide reference for scientific prevention and control of Omicron subvariant BA.5.
      原文链接:
    • 万方数据

    Genetic screening of 5 blood group systems of Li ethnic group in Hainan province and establishment of rare blood group bank

    FU Xiao?lingCAI Xing?quanXIA Lan
    6-12页
    查看更多>>摘要：Objective: To screen the blood group system genes of Duffy, Lutheran, Kidd, Diego, Dombrock blood group systems of Li ethnic group in Hainan Province and provide laboratory data for the rare blood group database in this area. Methods: The alleles of Duffy, Lutheran, Kidd, Diego, Dombrock blood group systems of 300 voluntary participants of Li ethnic group in Hainan were detected by sequence‑specific primer polymerase chain reaction , and the polymorphism was analyzed. Results: The allele frequencies of Duffy, Lutheran , Kidd, Diego, Dombrock blood group systems of Li ethnic groups in Hainan Province are 0.9583 for Fya, 0.0417 for Fyb, 0.8350 for Aua, 0.1650 for Aub, 0.4500 for Jka, 0.5500 for Jkb, 0.0667 for Dia, 0.9333 for Dib, 0.1017 for Doa and 0.8983 for Dob, respectively. The antigen incompatibility rates of Fya/Fyb, Aua/Aub, Jka/Jkb, Dia/Dib, Doa/Dob of Duffy, Lutheran, Kidd, Diego, Dombrock blood group systems were 7.67％, 23.76％, 37.25％, 11.67％ and 16.60％, respectively. Conclusion: The gene frequencies of Duffy, Lutheran, Kidd, Diego, Dombrock blood group systems of Li ethnic group in Hainan Province are polymorphic, and the antigen incompatibility rates of alleles are higher, which is quite different from that of other nationalities in China and with unique ethnic distribution characteristics.It is of great significance to establish the rare blood group database in this region.
      原文链接:
    • 万方数据
    • 维普

    Expression of Nrf2/ARE pathway in diabetic nephropathy and renal protection of Qihuang Bushen Xiezhuo Formula

    JIN Li?xiaZHANG Xiao?dongPAN ChaoLUAN Zhong?qiu...
    13-18页
    查看更多>>摘要：Objective: To investigate the effect of Qihuang Bushen Xiezhuo Formula on the expression of the nuclear factor E2 related factor 2(Nrf2)/ antioxidant response element (ARE) signaling pathway of human glomerular mesangial cells (HMC) in high glucose medium and its protective effect on oxidative stress. Methods: The HMC were cultured in vitro to prepare normal rat serum. The rats were intragastrically administered with irbesartan and Qihuang Bushen Xiezhuo Formula. The serum containing the drugs was prepared after the blood concentration was reached. The rats were divided into the control group, the high glucose group, the irbesartan group and the Qihuang Bushen Xiezhuo Formula group. The HMC of the control group was cultured with normal rat serum (10％ serum concentration) medium, while that of the high glucose group was cultured with high glucose medium of rat serum (10％ serum concentration). The irbesartan group and the Qihuang Bushen Xiezhuo Formula group were respectively treated with 10％ irbesartan and 10％ Qihuang Bushen Xiezhuo Formula in serum high glucose medium. After 48 h of culture, the relevant indicators were collected and detected. The mRNA expression levels of Nrf2, gamma‑glutamylcysteine synthetase(γ‑GCS) and superoxide dismutase(SOD) in each group were detected by real‑time PCR. The expressions of γ‑GCS and SOD were detected by immunohistochemistry. The protein expressions of γ‑GCS and SOD in HMC of each group were observed by Western Blot. Results: The expressions of Nrf2, γ‑GCS, SOD mRNA and protein in experimental cells of each group were low in the control group, while those in the high glucose group were decreased as compared with the control group (P<0.05). After interference of irbesartan and Qihuang Bushen Xiezhuo Formula the expressions were increased, and the increase in Qihuang Bushen Xiezhuo Formula group was more significant (P<0.05). Conclusion: Qihuang Bushen Xiezhuo Formula can improve HMC oxidative stress injury in high glucose culture, and its mechanism may be achieved by activating Nrf2 and its related downstream proteins γ‑GCS and SOD.
      原文链接:
    • 万方数据
    • 维普

    Based on the expression of HSP60 and HSP90, to explore the effect of Xuduan Zhongzi prescription on semen quality of oligoasthenospermia model rats

    ZHANG Ya?guangWANG Quan?shengHUANG Zi?yanChen Lu...
    19-24页
    查看更多>>摘要：Objective: To investigate the effect of Xuduan Zhongzi prescription on the expression of HSP60 and HSP90 in testicular spermatogenic cells and semen quality of oligozoospermia and asthenospermia model rats. Methods: A total of 40 SPF male rats were randomly divided into 10 blank control group and 30 model group. After successful modeling, the model group was divided into model control group, levocarnitine group and Xuduan Zhongzi prescription group with 10 rats in each group. The rats in levocarnitine group and Xuduan Zhongzi prescription group were given corresponding drugs by gavage according to the equivalent measurement of humanbody, the blank control group and model control group were given the same amount of normal saline by gavage, and the rats in each group were killed after 8 weeks of continuous administration. Take the left epididymis of each group for sperm quality detection, take the left testis of each group, observe the histomorphological changes by HE staining, and detect the expression of HSP60 and HSP90 in testicular tissue by immunohistochemistry and Western blot. Results: HE staining showed that compared with the blank control group, the convoluted tubules in the model group were significantly atrophic, degenerated and irregularly arranged, the spermatogenic epithelium showed a large number of vacuoles, Sertoli cell degeneration, obvious inflammatory infiltration in stromal cells, stromal cell proliferation, scattered rows of epithelial cells, a large number of exfoliation and degeneration, and sperm were rare. After the intervention of Xuduan Zhongzi prescription, the above lesions were significantly improved, the structure of seminiferous tubules was relatively regular and orderly, denser than that of the model group, sertoli cells and stromal cells increased significantly, the seminiferous epithelium was thicker than that of the model group, with rich layers, and a large number of sperm could be seen in the lumen. Immunohistochemistry and Western Blot showed that the expression of HSP60 and HSP90 increased significantly after the intervention of Xuduan Zhongzi prescription in model rats (P<0.01). The sperm quality test results showed that compared with the blank control group, the sperm concentration and activity rate in the model group decreased significantly, (P<0.01). After continuous seed cutting, the sperm concentration and activity rate of model rats were significantly increased, (P<0.01). Conclusion: Xuduan Zhongzi prescription may inhibit the apoptosis of testicular spermatogenic cells in oligoasthenospermia model rats by increasing the expression of HSP60 and HSP90, so as to improve sperm quality.
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    • 万方数据
    • 维普

    The mechanism of Astragalus‑Prunella vulgaris in the treatment of diabetic cardiomyopathy based on network pharmacology and molecular docking

    LIU Li?yingHUANG Ju?kaiLI Gao?biaoZHANG Li...
    25-35页
    查看更多>>摘要：Objective: To study the main chemical components and mechanism of Astragalus and Prunella vulgaris in the treatment of diabetes cardiomyopathy (DCM) based on network pharmacology and in vitro experiments. Methods: The main active components and prediction targets of Astragalus membranaceus and Prunella vulgaris herbal pairs were obtained by TCM Pharmacology database and analysis platform (TCMSP), and the disease genes were retrieved by genecards, OMIM, PharmGKB and drugbank databases. The disease and drug prediction targets were intersected to screen out common potential therapeutic targets. Cytoscape3.7.2 software was used to construct "drug component disease target" interaction network diagram; The PPI network of protein‑protein interaction was constructed by using string database. R software was used to analyze the function enrichment of GO and KEGG for drug disease common targets, and autodock Vina 1.1.2 for molecular docking. Finally, the specific mechanism of Astragalus and Prunella vulgaris medicated serum on high glucose stimulated cardiomyocytes was verified in vitro. H9c2 cardiomyocytes were divided into five groups: normal group: low glucose (5.5 mmol/L) culture group, model group: high glucose (33 mmol/L) culture group, 5％ serum group: high glucose+5％ Astragalus membranaceus Prunella vulgaris herb serum culture group, 10％ serum group: high glucose+10％ Astragalus membranaceus Prunella vulgaris herb serum culture group, 15％ serum group: Hg high glucose+15％ Astragalus membranaceus Prunella vulgaris herb serum culture group. MTT assay was used to detect the cell survival rate, and Western blot was used to detect the effect of Astragalus and Prunella vulgaris medicated serum on the expression of AKT1, p‑AKT1, MAPK14 and p‑MAPK14 proteins. Results: In this study, 31 active components of Astragalus and Prunella vulgaris were screened, involving 157 targets of diabetes cardiomyopathy and 178 related signal pathways. The results of network analysis showed that Astragalus and Prunella vulgaris herbs may play a role in the treatment of DCM by acting on key targets such as AKT1, FOS, MAPK1, MAPK8, MAPK14, Jun and key pathways such as PI3K‑AKT. Molecular docking showed that Astragalus membranaceus and Prunella vulgaris medicine had good binding between the active components luteolin, quercetin, pistil isoflavone, kaempferol and key targets such as AKT1, MAPK14, MAPK1, FOS, mapk8 and Jun, and the Vina score of luteolin and AKT1 was the lowest. The results in vitro showed that Astragalus and Prunella vulgaris medicated serum significantly improved the inhibition of H9c2 cardiomyocyte proliferation induced by high glucose, and increased the phosphorylation levels of AKT1 and MAPK14 proteins to play a role in the treatment of DCM. Conclusion: Astragalus and Prunella vulgaris have the characteristics of multi‑target and multi‑channel in the treatment of DCM. Its mechanism may be related to the regulation of the protein expression of p‑AKT1 and p‑MAPK14. These findings provide a new idea and basis for further experimental study on the mechanism of Astragalus and Prunella vulgaris in the treatment of diabetes cardiomyopathy.
      原文链接:
    • 万方数据

    Identification of genes related to tubulointerstitial injury in type 2 diabetic nephropathy based on bioinformatics and machine learning

    SU Jia?mingPENG JingCHEN Hai?minZHOU Ying...
    36-44页
    查看更多>>摘要：Objective: To explore the genes related to renal tubulointerstitial injury in DN and to elucidate their underlying mechanism by using bioinformatics multi‑chip joint analysis and machine learning technology, so as to provide new ideas for the diagnosis and treatment of DN. Methods: Four gene expression datasets of DN tubulointerstitial tissues were retrieved from the GEO database. GSE30122, GSE47185 and GSE99340 were used as the combined microarray datasets, and GSE104954 was used as the independent verification datasets. The differentially expressed genes (DEGs) were identified by R language, and Gene Ontology (GO) enrichment, KEGG pathway enrichment, Gene Set Enrichment Analysis (GSEA) and Immune Cell Infiltration Analysis were performed. Furthermore, LASSO regression, SVM‑RFE and RF machine learning algorithm were used to screen core genes, while external validation and Receiver Operating Curve (ROC) analysis as well as the model of prediction nomogram were performed. Finally, the influence of the clinical characteristics of DN patients was explored by Nephroseq. Results: A total of 107 DEGs were obtained, enrichment analysis revealed that the tubulointerstitial injury in DN was mainly involved in adaptive immune response, lymphocyte mediated immunity, regulation of immune effector process and immune‑inflammatory pathways such as staphylococcus aureus infection, complement and coagulation cascades, phagosomes, and Th1 and Th2 cell differentiation. In addition, cell adhesion molecule, cytokine‑cytokine receptor interaction and ECM‑receptor interaction pathways were also significantly enriched. Memory resting CD4 T cells, γδ T cells, resting mast cells and neutrophil cells were up‑regulated, while CD8 T cells were down‑regulated. Machine learning identified MARCKSL1, CX3CR1, FSTL1, AGR2, GADD45B as core genes with good diagnostic and predictive efficacy. Conclusion: The key pathological mechanism of tubulointerstitial injury in DN is immune disorder, inflammatory reaction, cytokine action and extracellular matrix deposition. Moreover, MARCKSL1, CX3CR1, FSTL1 may be the potential biomarkers for the diagnosis and prediction of DN.
      原文链接:
    • 万方数据
    • 维普

    Meta‑analysis of risk factors for PTDM after renal transplantation in China in the past 10 years

    XIA Man-chengZHU Lian-yunLV ding-yangZHOU Hui-yu...
    45-55页
    查看更多>>摘要：Objective: To systematically evaluate the main risk factors of post‑transplant diabetes mellitus (PTDM) after renal transplantation in China in the past 10 years. Methods: CNKI, Wanfang, VIP, and PubMed were searched to collect the related literatures on risk factors of PTDM after renal transplantation published by Chinese scholars from January 2010 to October 2020. The data were extracted and Meta‑analysis was performed by Revman 5.3 software. Results: A total of 18 case‑control studies were included, involving 5458 patients. There were 1106 PTDM cases after kidney transplantation and 4352 cases without PTDM after kidney transplantation. Meta‑analysis results showed age [MD=6.09, P<0.00001], gender [OR=1.22, P=0.02], family history of diabetes [OR=5.56, P<0.0001], source of donor kidney [OR=1.87, P<0.0001], BMI [MD =1.76, P<0.00001], HBV infection [OR=2.52, P=0.04], HCV infection [OR=2.55, P<0.0001], CMV infection [OR=1.81, P=0.008], Cyclosporin A [OR=0.51, P=0.04], tacrolimus [OR=2.34, P=0.003], acute rejection [OR=2.72, P<0.00001], and smoking history [OR=2.01, P=0.0006] were associated with PTDM after renal transplantation in China. Conclusion: Age, gender, family history of diabetes, source of kidney donors, BMI, HBV, HCV, CMV, tacrolimus, acute rejection, and smoking history are risk factors for PTDM after kidney transplantation in China. Cyclosporin A is protective factors of PTDM after kidney transplantation in China. These factors are worthy of attention by relevant clinical workers in our country.
      原文链接:
    • 万方数据

    Clinical evaluation of Fufangkushen combined with oxaliplatin in the treatment of advanced colorectal cancer

    ZHOU TongWANG ShuoHU Shuai-hangLI Zheng...
    56-66页
    查看更多>>摘要：Objective: To systematically evaluate the efficacy and safety of compound Kushen injection combined with oxaliplatin chemotherapy in the treatment of advanced colorectal cancer. Methods: We searched PubMed, EMbase, the Cochrane Library, CNKI, VIP and Wan Fang database, SinoMed to collect compound Kushen injection combined with chemotherapy oxaliplatin into treatment of advanced colorectal cancer in randomised controlled trials; the databases weresearched from inception to December 2020. Meta‑analysis of the included studies was performed using RevMan 5.4. Results: A total of 34 randomized controlled trials involving 2664 patients with colorectal cancer were included. Results of Meta‑analysis showed that compound Kushen injection combined with oxaliplatin chemotherapy regimen improved the objective response rate of tumor [RR=1.40, 95％CI(1.29,1.51), P<0.00001] and disease control rate [RR=1.12, 95％CI(1.08,1,16), P 0.00001] improved the quality of life [RR=1.24, 95％CI(1.14,1.36), P<0.00001], and significantly reduced the incidence of leukopenia [RR=0.35,95％CI(0.23,0.52), P<0.00001] and the incidence of diarrhea [RR=0.36, 95％CI(0.19,0.70), P=0.003], and improved the immune function of patients (CD3+, CD4+, CD4+/CD8+, NK cell levels). However, compared to the control group, the levels of CD8+ cells were decreased in the experimental group. Conclusion: Compound Kushen injection combined with oxaliplatin chemotherapy regimen can significantly improve the clinical efficacy of advanced colorectal cancer patients, improve the quality of life of patients, reduce the occurrence of adverse reactions, and has good efficacy and safety comparison with oxaliplatin chemotherapy regimen alone.
      原文链接:
    • 万方数据
    • 维普

    Airway maintenance of critically ill patients

    HE Ming?xinZHOU Xiang?dongZHOU MingLUO Ding...
    67-71页
    查看更多>>摘要：With the advancement of medical technology, artificial airway has been widely used in modern medicine in our country, especially in the treatment and rescue of critically ill patients. However, the establishment of an artificial airway will also affect the original anatomical structure and normal function of the airway, which will cause a series of complications and pose a serious threat to the prognosis of patients. Therefore, effective airway maintenance can not only prevent the occurrence of complications, reduce the physical and mental trauma to the patient, but also optimize the treatment effect. At this stage, domestic and foreign airway maintenance strategies have shown varying degrees of new cognition. This article reviews the latest research status of airway maintenance strateges at home and abroad, hoping to provide clinicians with a reference for the latest cognition in airway maintenance strategies.
      原文链接:
    • 万方数据
    • 维普

    Research progress on the mechanism of treating chronic kidney disease by invigorating the kidney and removing toxic

    KANG Lei?leiZHOU En?chao
    72-76页
    查看更多>>摘要：Chronic kidney disease is a general term for various types of renal diseases characterized by a long course of disease and difficulty in early detection. It is one of the social diseases that need to be solved urgently and its pathogenesis and treatment have always been a hotspot for researchers. Studies have shown that traditional Chinese medicine (TCM) can effectively delay the progress of chronic kidney disease. According to the clinical experience of national famous TCM physicians, based on its pathogenesis of "kidney deficiency as the foundation and poison as the target", chronic kidney disease can be treated by invigorating the kidney and removing toxic. Based on the above background, this paper summarizes the research progress of invigorating the kidney and removing toxic in delaying chronic kidney disease from multiple mechanisms and pathways in modern research.
      原文链接:
    • 万方数据
    • 维普