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海南医科大学学报（英文版）
海南医科大学学报（英文版）

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海南医科大学学报（英文版）/Journal Journal of Hainan Medical University
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    Novel coronavirus "Omicron subtype variant strain BA.2": The latest research progress

    You-Cheng XieYin-Nan KangChun GaoShan-Shan Liu...
    1-4页
    查看更多>>摘要：Since the outbreak of COVID-19, with the emergence of a series of novel coronavirus variants of different types, COVID-19 has continued to spread across the globe, posing a huge threat to the lives and health of people around the world and posing a severe challenge to global public health security. Recently, the World Health Organization (WHO) announced that novel coronavirus variant strain "Omicron" subtype variant strain BA.2 has been found in 57 countries and regions. It has higher vaccine and antibody tolerance and is more infectious than omicron (B.1.1.529). In this paper, we briefly review the recent studies on the omicron subtype variant strain BA.2.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Mechanism of hesperidin improving myocardial ischemia/reperfusion injury in type 2 diabetic rats through SIRT1/Nrf2/HO-1 signaling pathway

    Zhen-Wang MaDe-You JiangBing-Cheng HuXing-Xing Yuan...
    5-10页
    查看更多>>摘要：Objective: To observe the protective effect of hesperidin on myocardial ischemia/ reperfusion injury in type 2 diabetes mellitus and its effect on SIRT1/Nrf2/HO-1 signaling pathway. Methods: 50 Sprague-Dawley (SD) rats were randomly assigned to the normal control group (NC), model group, ischemia-reperfusion group (IR), hesperidin group, SIRT1 inhibitor group and hesperidin plus SIRT1 inhibitor group. In addition to NC, the rats in the remaining groups were replicated by intraperitoneal of high-fat diet combined with injection of streptozotocin for type 2 diabetic rats. After then, the myocardial ischemia/reperfusion injury (MIRI) rat model was established by LAd for 30 minutes with 2 hours reperfusion. He staining was used to observe the pathological changes of myocardial tissue, and the levels of serum LDH, CK-MB and SOD, GSH and MDA in myocardial tissue were detected by kit methods, and the expression abundance of related proteins in 4-HNE and SIRT1/Nrf2/HO-1 signal pathway were detected by immunohistochemistry and Western blot; Results: Hesperidin could significantly inhibit cardiomyocyte necrosis and inflammatory cell infiltration, reduce LDH activity, CK-MB and MDA level, and increase SOD activity, GSH and 4-HNE level, the differences were statistically significant when compared with IR group (P<0.01). In addition, compared with the ischemia-reperfusion group, the expressions of SIRT1, Nrf2 and HO-1 proteins in hesperidin group were significantly up-regulated, the differences were statistically significant (P<0.01); Conclusion: Hesperidin inhibits oxidative stress by activating SIRT1/Nrf2/HO-1 signaling pathway, and play a protective effect of myocardial ischemia reperfusion injury in diabetic rats.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Mechanism of Xingnaojing injection intervention in cerebral ischemia-reperfusion rat model based on GC-MS metabolomics

    Hao-Qi LiuHan-Lai ZhangYuan-Yuan LiKe Song...
    11-16页
    查看更多>>摘要：Objective: To investigate the protective effect of Xingnaojing injection on cerebral ischemia- reperfusion in rats and its metabolic pathway and mechanism. Methods: The cerebral ischemia reperfusion model of rats was established by suture occlusion. After successful model evaluation, he rats were randomly divided into model group and Xingnaojing group with eight rats in each group. In the sham operation group, only blood vessel separation was performed without embolization. Xingnaojing group was given intraperitoneal injection, model group and sham operation group were given the same dose of normal saline, twice a day. Three days later,HE staining and GC-MS metabolomics were used to detect the changes of endogenous metabolites in the rat brain tissue. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to screen out differential metabolites and analyze their metabolic pathways. Results: Endogenous metabolites were disturbed after cerebral ischemia-reperfusion injury in rats. Seventy-one different metabolites were screened from the model group and the sham group, of which three were down-regulated and sixty-eight were up-regulated. Eighty-eight different metabolites were found between Xingnaojing group and sham operation group, among which eight were down-regulated and eighty up-regulated. After screening of Xingnaojing group and model group, twelve different metabolites were obtained, among which seven were down-regulated and five up-regulated. By analyzing the differences of metabolites, Xingnaojing injection was considered to be involved in the metabolic pathway after cerebral ischemia-reperfusion in rats, including amino acid metabolism (beta alanine metabolism, alanine, glutamic acid and aspartic acid metabolism, histidine metabolism, arginine and proline metabolism), glutathione metabolism, pyrimidine metabolism, ABC transporter, nitrogen metabolism and other metabolic pathways. Conclusion: Xingnaojing injection can restore the levels of metabolites in cerebral ischemia-reperfusion rats in certain degrees, mainly through amino acid metabolism, ABC transporter, glutathione metabolism and other metabolic pathways to regulate cerebral ischemia-reperfusion injury in rats.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Randomized controlled trial of Qing Gan Huo Xue Prescription in the treatment of alcoholic liver cirrhosis

    Jie-Lu PanLian-Jun XingJi ZhangXiao Yu...
    17-22页
    查看更多>>摘要：Objective: To evaluate the efficacy of Qing Gan Huo Xue Prescription(QGHXP)in the treatment of patients with alcoholic liver cirrhosis(ALC)of damp and heat stasis syndrome. Methods: A total of 69 patients with ALC were randomly divided into TCM group(n=35) and control group(n=34). The TCM group was given QGHXP 1 pack TID orally. Control group received polyene phosphatidylcholine capsule 456 mg TID for 24 weeks. The observation measurements are symptom efficacy rate,serum level of liver enzyme,and non-invasive liver cirrhosis evaluation,including liver stiffness measurement(LSM)examininged by FibroTouch,APRI score,FIB-4 index and Maddrey discriminant function. Results: The symptom efficacy rate of the experimental group and the control group was 85.70％ and 61.80％(P=0.024). Liver enzyme levels(serum ALP,γ-GT, AST and ALT)of TCM group were lower than those of control group(P<0.05). LSM of TCM group was reduced after treatment,and was significant lower than control group(14.19±1.49)vs.(15.06±1.24)(P<0.05). The APRI scores,FIB-4 index and Maddrey discriminant functions of TCM group were lower than those of control group(P<0.05). Conclusion: QGHXP is an effective alternative for the treatment of damp and heat stasis syndrome of ALC in improving liver function and clinical symptoms.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Clinical significance of the detection of Rh blood group antigens and irregular antibodies in pregnant women with a second pregnancy

    Xiao-Ling FuXing-Dan ZhaoAi-Han WengSu-Jiao Li...
    23-28页
    查看更多>>摘要：Objective: To investigate the phenotype distribution of five antigens of Rh blood group system and the specificity of Rh blood group irregular antibodies in pregnant women with second child.To analyze the relationship between Rh blood group antibody and hemolytic disease of the newborn(HDN) in second-child pregnant women, and to provide laboratory basis for the diagnosis and treatment of hemolytic disease of the newborn(Rh-HDN). Methods: 500 pregnant women with second child were collected as the study group and 500 pregnant women with first pregnancy as the control group(all pregnant women underwent obstetric examination in the integrated obsteric clinic of our hospital from January 2020 to January 2021).To detectethe Rh blood group antigens (D, C, c, E,e) of the two groups of samples,screene the irregular antibodies, identify the specificity of irregular antibodies, determine the titer and record the hemolytic disease of the newborn of pregnant women with positive Rh blood group antibodies. Results: There were 11 Rh phenotypes in the pregnant women with second child in the study group: CCDee(152cases,30.4％),CcDEe(136cases,27.2％)CcDee(84cases,16.8％) ,ccDEE(30cases,6％),ccDee(31cases,6.2％),CCDEe(14cases,2.8％),ccDEe(9cases,1.8％),cc dee(18cases,3.6％),CCDEE(2cases,0.4％),CcdEe(12cases,2.4％), Ccdee(6cases,1.2％),CCd ee(6cases,1.2％). A total of 42 cases (8.4％) in the pregnant women with second child were negative for RhD. There were 10 Rh phenotypes in the pregnant women with first pregnancy in the control group: CCDee (144cases,28.8％), CcDEe(138cases,27.6％),CcDee(90cases,18％),ccDEE(42cases,8.4％),ccDee(28cases,5.6％),CCDEe(10cases,2％),ccDEe(8cases,1.6％),cc dee(19cases,3.8％),CCDEE(1cases,0.2％),CcdEe(11cases,2.2％),Ccdee (9cases,1.8％).A total of 39 cases (7.8％) in the pregnant women with first pregnancy were negative for RhD.In the pregnant women with second child in the study group, the positive rate of irregular antibody screening was 4.0％ (20/500), and the specificity of Rh blood group antibodies was found as follows:anti-E 1.8％(9/500),anti-D 1.4％ (7/500),anti-C 0.4％(2/500) and anti-Ec 0.4％(2/500). The positive rate of irregular antibody screening in the pregnant women with first pregnancy in the control group was 0, and the difference between the two groups was statistically significant (P<0.05).Rh-HDN was found in 10 newborns (2％) of the 20 women with positive irregular antibodies in the pregnant women with second child, and the antibody titer during pregnancy was more than 32. No Rh-HDN occurred in newborns in the pregnant women with first pregnancy, and the difference between the two groups was statistically significant (P<0.05). Conclusion: Pregnancy stimulation can increase the probability of irregular antibodies in pregnant women, and irregular antibodies in Rh blood group can easily cause Rh-HDN, so attention should be paid to routine detection of five antigens of Rh blood group and irregular antibody screening during prenatal examination. It is helpful for the early detection of Rh-blood irregular antibodies and the assessment of fetal or neonatal risk of Rh-HDN.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Meta-analysis of efficacy and safety of Endostar combined with vinorelbine and cisplatin in the treatment of non-small cell lung cancer

    Ze-Yun LiQing-Xia LanCheng-Xin LiuZhe Sun...
    29-35页
    查看更多>>摘要：Objective: To systematically evaluate the efficacy and safety of rh-endostain(YH-16,Endostar) combined with vinorelbine and cisplatin (NP regimen) in the treatment of non-small cell lung cancer(NSCLC),and to provide evidence-based reference for clinical drug use. Methods: Retrieved from PubMed, EMBASE, the Cochrane Library, Clinical Trials, CNKI, VIP and Wan Fang database, randomized controlled trials(RCT)about YH-16 combined with NP regimen(NPY regimen, trial group) vs. NP regimen(control group)for NSCLC were collected. After screening the literature and extracting the data, the two persons evaluated the quality of the included studies, and used Rev Man 5.3 software to merge effect size. Results: A total of 18 articles were included, with a total of 2051 patients. Results of Meta-analysis showed that response rate [RR=1.66,95％CI(1.44,1.91),P<0.00001]、 clinical benefit rate [RR=1.21,95％CI(1.14,1.29),P<0.00001] and quality of life improvement rate [RR=3.42,95％CI(2.45, 4.79),P<0.00001] of trial group were significantly higher than those of control group. Besides, the serum CEA level [MD=-4.78,95％CI(-7.11,-2.46),P<0.0001] and CA125 level [MD=-16.44,95％CI(-20.83,-12.05),P<0.00001] of trial group were significantly lower than that of control group. There was no statistical significance in the 1-year survival rate and the incidence of myelosuppression, cardiotoxicity, gastrointestinal reaction, damage to the kidneys and liver, and alopecia (P>0.05). Conclusion: Compared with NP regimen alone, NPY regimen can improve the efficacy and quality of life of NSCLC patients, reduce the level of tumor markers, and does not increase the occurrence of adverse reactions, and has good efficacy and safety. However, the existing evidence shows that NPY regimen has the same effect as NP regimen alone in improving the 1-year survival rate of patients. The above conclusions need to be confirmed by further studies.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Efficacy and safety of Nephritis rehabilitation tablet combined with Valsartan for chronic glomerulonephritis: A system review and meta-analysis

    Qiao LiuTai-Wei DongFeng GaoYan-Li Guo...
    36-44页
    查看更多>>摘要：Objective: To systematically evaluate the efficacy and safety of Nephritis Rehabilitation Tablet (NRT) combined with valsartan in treatment of chronic glomerulonephritis (CGN). Methods: Computer search databases such as CNKI, CBM, VIP, wanfang, Embase, PubMed and Cochrane library, and find all randomized controlled trials (RCTs) comparing NRT combined with valsartan versus valsartan in treatment of chronic glomerulonephritis. The search time limit is to build the database until October 2020. RCTs were screened according to the inclusion and exclusion criteria. After data extraction and quality assessment, the Cochrane risk of bias tool was used to evaluate the methodological quality of these studies. Meta-analysis was performed by Review Manager 5.2, and GRADE system for evidence quality evaluation. Results: We have identified a total of 24 eligible RCTs with 2082 participants and completed a meta-analysis based on these RCTs. The results of the meta-analysis showed that compared with valsartan, NRT combined with valsartan treatment showed effective curative effect in terms of effective treatment rate (OR = 4.72; 95％CI, 3.67, 6.08; P < 0.00001), 24h urine protein quantification (MD = -0.52; 95％CI, -0.59, -0.44; P < 0.00001), serum creatinine (Scr) (MD = -10.33; 95％CI, -14.00, -6.66; P < 0.00001), Systolic blood pressure (SBP) (MD = -11.42; 95％CI, -17.67, -5.17; P = 0.0003), Diastolic blood pressure (DBP) (MD = -6.28; 95％CI, -9.14, -3.42; P < 0.0001), Blood urine nitrogen (BUN) (MD = -0.02; 95％ CI, -0.41, 0.37; P = 0.93), plasma albumin (ALB) (MD = 5.05; 95％ CI, 4.27, 5.84; P < 0.00001), and adverse reactions (OR = 0.93, 95％ CI, 0.54, 1.60; P = 0.78). No serious adverse events were mentioned in these studies. And based on the results of the systematic review, the GRADE system recommended ranking method was used to evaluate the quality of evidence and the recommendation level. The results showed that the level of evidence was moderate and the recommendation intensity was weak recommendation. Conclusions: NRT combined with valsartan has a significant effect on the treatment of CGN, and the treatment effect is better than valsartan alone. There are no obvious adverse reactions during the treatment process. However, due to the generally low quality of the literature included in these studies, and the variability of the evaluation methods of each study, morelarge samples, multi-center, high-quality samples are still needed RCTs are further verified.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Study on the mechanism of "Sangu Decoction" in the treatment of bone metastatic carcinoma

    Meng-Xia YangYun MaoLi-Yu HeMin Liang...
    45-50页
    查看更多>>摘要：Objective: To study the mechanism of "Sangu Decoction" in the treatment of bone metastatic carcinoma by network pharmacology. Methods: TCMSP, TCMID and TCM Database@ Taiwan databases and literature search were used to screen the main effective components of drugs. Swiss Target and TCMSP databases were used to search the potential therapeutic targets of "Sangu Decoction". DisGeNet and Drugbank databases were used to search the genes of bone metastatic carcinoma. Drug action targets and disease genes were mapped. Cytoscape 3.8.1 software was used to visualize and screen out the core genes. GO and KEGG pathway analysis was performed on potential therapeutic targets. Results: There were 29 main active ingredients in "Sangu Decoction", which contained 413 target proteins. There are 773 disease targets of bone metastatic carcinoma, of which 112 are potential targets of "Sangu Decoction" for the treatment of bone metastatic carcinoma. Through GO and KEGG pathway analysis, it was found that "Sangu Decoction" exerted anti-cancer, inhibiting bone metastasis and immune regulation mechanism through TNF, ErbB, FoxO, PI3K-Akt, Toll-like Receptor, NOD-like Receptor, Rap1 and other signaling pathways in the treatment of bone metastatic carcinoma. The key genes of "Sangu Decoction" in treating bone metastatic carcinoma are VEGFA, AKT1, IL6, TP53, MAPK3, SRC, EGFR and CASP3 and so on. Conclusion: In this study, we constructed a a multi-level interaction "traditional Chinese medicine -compound- target -pathway" network through network pharmacology, and found that the mechanism of "Sangu Decoction" in the treatment of bone metastasis cancer involves multiple targets and pathways, which may be related to anti-cancer, inhibition of bone metastasis, regulation of immunity and so on.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Research on the effect of Bletilla striata and the mechanism of the treatment of bronchoplumonary inflammation based on network pharmacology

    Jie WangXiang-Dong ZhouQi LiPan-Hong Jia...
    51-58页
    查看更多>>摘要：Objective: To explore the effect of active compounds of Bletilla Striata, and to predict its mechanism of the treatment of bronchoplumonary inflammation. Methods: Retrieved active compounds of Bletilla Striata from relevant databases include TCMSP,while retrieved action targets of active compounds and bronchoplumonary inflammation. Received corresponding gene symbols of targets by querying UniProt database. Then drew the compound-target network of Bletilla Striata and action targets network between active compounds and bronchoplumonary inflammation using Cytoscape 3.2.1. Subsequently performed GO functional and KEGG enrichment analysis of key targets through relevent databases including Metascape, meanwhile, analysed Protein-protein interaction using STRING. Results: Bletilla Striata contained 29 active components and 632 corresponding targets. The more important targets included AURKA, BRAF, RAF1, MMP1 and ESR2, 213 in total. GO functional enrichment analysis resulted in 2,398 items (P < 0.05), including 2096 biological process (BP) items , 133 cellular component (CC) items, 169 molecular function (MF) items. KEGG enrichment analysis resulted in 160 signal pathways items (P < 0.05). There were 33 common targets between 335 action targets of bronchoplumonary inflammation and 95 more important active components of Bletilla Striata. GO functional enrichment analysis resulted in 547 items (P < 0.05), including 499 BP items , 19 CC items, 29 MF items. KEGG enrichment analysis resulted in 46 signal pathways items (P < 0.05), mainly involving the IL-17 signaling pathways, platelet activation, endocrine resistance, Fc epsilon RI signaling pathway, Th17 cell differentiation, etc. 6 core targets including MAPK14, MMP9, HSP90AA1, ROCK2, PRKCB and PTGS2 were obtained by PPI analysis. They were involved in IL-17 signaling pathway, platelet activation, leukocyte migration, VEGF signaling pathway and Th17 cell differentiation respectively. Conclusion: The study explains "multi-component-multi-target-multi-pathway-multi-disease" is the characteristic of Bletilla Striata using network pharmacology, and predict the active compounds of Bletilla Striata can be used for treatment of bronchoplumonary inflammation with the effect of regulating to anti-inflammation and tissue repair and remoding. This study theoretically verified the functional utility of Bletilla Striata and provided scientific basis and reference direction for further experimental research.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Research progress on the role of gut microbiota dysregulation in the pathogenesis of diabetic nephropathy

    Han WangDun-Fang WangHong-Xin SongXu-Ran Ma...
    59-65页
    查看更多>>摘要：There are about over 100 trillion microbial cells in human gut, which affect the nutritional, metabolic, physiological and immune functions of the host. This paper reviews the differences in gut microbiota between patients with diabetic nephropathy (DN) and healthy people. These differences lead to the disorder of symbiotic relationship, which may have induced the progression of DN, as well as targeted interventions to reconstruct the symbiotic relationship. Recent studies have found that endotoxin from intestinal bacteria and a large number of toxic metabolites were produced by fermentation of gut microbiota, such as trimethylamine-N-oxide, indoxyl sulfate and p-cresol sulfate, leading to the disruption of intestinal barrier function. Endotoxin and bacterial metabolites, entering the systemic circulation, were involved in DN progression by mediating inflammatory responses, renin-angiotensin-system and vascular injury. The reduction of some beneficial bacterial metabolites in DN patients, such as short-chain fatty acids, would weak body energy metabolism and destroy glucose homeostasis. In addition, gut microbiota is essential for the conversion of bile acids, and plays an important role in the development of DN by synthesizing secondary bile acids and regulating glucose and metabolic balance through foresaid X receptor (FXR) and G protein-coupled bile acid receptor (TGR5). Animal and clinical studies have revealed that probiotics, prebiotics, fecal microbiota transplantation, and Chinese medicine intervention may have potential therapeutic effects in maintaining a metabolically balanced gut microbiota to reduce the progression of DN, end-stage renal disease and cardiovascular complications.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普