查看更多>>摘要:Accumulating evidence indicates that the Brain-derived neurotrophic pathway play important roles in depression.Previous studies in our laboratory have demonstrated that YLSPA produced antidepressant-like action in Kunming mice with various mouse models of behavioral despair (FST and TST). The present study aimed to examine whether YLSPA could affect the chronic unpredictable mild stress (CUMS)-induced depression in mice. The mechanism(s) underlying the antidepressant-like action was investigated by measuring brain-derived neurotrophic factor (BDNF) ,its receptor(TrkB) ,the relevant factor of pathway CREB and pCREB using Immunohistochemistry.CUMS, being lasted for 3 weeks, caused depression-like behavior in mice, as indicated by the significant decrease in sucrose consumption and spontaneous motor activity. Whereas CUMS produced depressive-like behaviors and decreased the expression of BDNF , TrkB (p < 0.05 or p <0.01), accompanied by a decreased expression of both CREB and pCREB in the dentate gyrus region of the hippocampus (p < 0.05 or p <0.01). On day 21, CUMS resulted in a decreased number of apoptosis cells and the level of bax in the dentate gyrus zone (p < 0.05 or p <0.01) while taken an increased in the expression of Bcl-2.Daily intragastric administration of YLSPA(300,600 mg/kg) during three weeks of CUMS significantly suppressed behavioral and biochemical changes induced by CUMS.The results suggest that the antidepressant-like action of YLSPA is likely mediated by modulating the function of increasing the expression of BDNF in brain tissues and related factors.
查看更多>>摘要:目的:探讨动力髋螺钉(DSH dynamic hip screw)与股骨近端防旋髓内钉(PFNA proximal femoral nail antirotation blade)两种内固定手术方式在老年股骨粗隆间骨折治疗应用中的临床效果.方法:选取符合手术治疗的老年股骨粗隆间骨折患者86例,通过对PFNA组及DHS组患者的年龄、致伤因素、手术前合并症、切口长度、手术时间、术中出血量、术后引流量、术后下地时间进行对比,并通过髋关节Harris评分比较髋关节功能的优良率.结果:PFNA组切口长度、手术时间、术中出血量、术后引流量、术后下地时间均优于DHS组;髋关节Harris评分两组无差异.结论:DHS和PFNA都是治疗股骨粗隆间骨折的有效内固定方式,PFNA具有操作简便、手术时间短、出血量少等特点.