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食品品质与安全研究（英文版）
食品品质与安全研究（英文版）
食品品质与安全研究（英文版）/Journal Food Quality and SafetyCSCDSCI
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    Inhibitory effect and mechanism of tannic acid against two starch digestive enzymes

    钟毓秀倪德让杨玉波李元一...
    115-123页
    查看更多>>摘要：Backgrounds:Tannic acid(TA),as a plant-derived phenolic substance,is involved in regulating the activity of starch digestive enzymes,but its underlying mechanism remains unclear.Methods and Results:In the present study,inhibition rate and inhibition kinetics assays were performed and confirmed that TA had a strong inhibitory effect on both α-amylase and α-glucosidase with IC50 values of 0.1585 mg/mL and 0.00542 mg/mL,respectively,through a mixed inhibition mode.The secondary structures of both enzymes were confirmed to be modified by TA through circular dichroism(CD)spectra.Fluorescence quenching analysis revealed that the interaction between TA and two enzymes was a static process of pontaneous complex for-mation.Finally,molecular docking revealed that non-covalent bonds were the main interaction forces between TA and both enzymes.Conclusions:Thus,TA was a promising candidate for the inhibition of starch-digesting enzymes,and the present research provided insight into postprandial glucose regulation through polyphenols.
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    Comparative metabolic analysis and antioxidant properties of purple and white wheat grains:implications for developing functional wheat varieties

    孙楠楠张至中徐月婷徐正圆...
    124-134页
    查看更多>>摘要：Objectives:Pigmented wheat has garnered increased attention due to its elevated antioxidant activity.This study aimed to compare the meta-bolic components and antioxidant properties of purple and white wheat grains.Materials and Methods:Metabolic analysis identified a total of 405 secondary metabolites using three extraction methods:free,acid,and alkali hydrolysis.Results:The results revealed that,compared with white wheat,purple wheat exhibited significantly higher levels of total polyphenols,total fla-vonoids,and antioxidant activity.Notably,there were substantial variations in 90,80,and 73 secondary metabolites between white and purple wheat under the respective extraction methods.The contents of hispidulin-8-C-(2"-O-glucosyl)glucoside,hispidulin-8-C-glucoside,diosmetin-6-C-glucoside,and scoparin decreased across all groups,while the contents of peonidin-3-O-glucoside,keracyanin,kuromanin cyanidin-3-O-glucoside,and cyanidin-3-O-(6"-O-malonyl)glucoside increased.Conclusions:These findings suggest that pathways associated with anthocyanin,flavone,and flavonol biosynthesis were influenced.Furthermore,each extraction method revealed distinct secondary metabolites.Acid and alkali hydrolysis upregulated 16 and 3 metabolites in purple wheat,and 20 and 3 metabolites in white wheat,respectively.Acid and alkaline hydrolysis also identified indole-3-carboxylic acid,vanillic acid,pyridoxine,and other metabolites with antioxidant and antihypertensive effects in both white and purple wheat.This indicates that acid and alkaline hydrolysis can dissociate a greater variety and quantity of differential metabolites.These findings offer valuable insights for breeders seeking to develop wheat varieties with enhanced functional nutritional value.
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    • NETL
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    Discrimination of pharmacodynamic components and volatile compounds of Poria cocos cultivated with five different materials based on GC-IMS analysis

    Yinan WangFei ZhouHui WangJinping Zhang...
    135-144页
    查看更多>>摘要：Objectives:The chemical constituents of Poria cocos grown with different substrates vary significantly;thus,identifying and comparing their biomarkers are important.Materials and Methods:Herein,the chemical constituents of Poria cocos obtained with five different substrates were assessed using gas chromatography-ion mobility spectrometry(GC-IMS),high-performance liquid chromatography and multivariate statistical analysis.Results:The content of moisture,ash,alcohol-soluble matter,and heavy metals,except for those of the miscellaneous wood Poria cocos,conform to the specifications defined in the Chinese Pharmacopoeia(Edition 2020),and the polysaccharide contents are all greater than 57％.Conclusions:Based on GC-IMS and the established fingerprints,87 compounds were detected,70 of which were identified in each group.Multivariate statistical analysis revealed seven compounds(two esters,three alcohols,and two aldehydes),which could be considered as po-tential marker compounds for discrimination.
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    Comprehensive characterization and detection of nut allergens in bakery foods using Q-TOF mass spectrometry and bioinformatics

    Daokun XuHaolun HuangZhen LiuYumei Wang...
    145-157页
    查看更多>>摘要：Food allergy is a growing health issue worldwide and the demand for sensitive,robust and high-throughput analytical methods is rising.In recent years,mass spectrometry-based methods have been established for multiple food allergen detection.In the present study,a novel method was developed for the simultaneous detection of almond,cashew,peanut,and walnut allergens in bakery foods using liquid chromatography-mass spectrometry.Proteins unique to these four ingredients were extracted,followed by trypsin digestion,quadrupole time-of-flight(Q-TOF)mass spectrometry and bioinformatics analysis.The raw data were processed by de-novo sequencing module plus PEAKS DB(database search)module of the PEAKS software to screen peptides specific to each nut species.The thermal stability and uniqueness of these candidate pep-tides were further verified using triple quadrupole mass spectrometry(QQQ-MS)in multiple reaction monitoring(MRM)mode.Each nut species was represented by four peptides,all of which were validated for label-free quantification(LFQ).Calibration curves were constructed with good linearity and correlation coefficient(r2)greater than 0.99.The limits of detection(LODs)were determined to range from 0.11 to 0.31 mg/kg,and were compared with the reference doses proposed by Voluntary Incidental Trace Allergen Labelling(VITAL).The recoveries of the developed method in incurred bakery food matrices ranged from 72.5％to 92.1％with relative standard deviations(RSD)of＜5.2％.The detection of un-declared allergens in commercial bakery food samples confirmed the presence of these allergens.In conclusion,this method provides insight into the qualitative and quantitative detection of trace levels of nut allergens in bakery foods.
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    Fabrication of telechelic DNA-bridged food emulsion gel as edible ink for 3D printing

    Ruyuan ZhangHao HuangRong AiDong Li...
    158-165页
    查看更多>>摘要：Objectives:Interdroplet interactions affect the properties and textures of emulsions.In this study,we creatively introduced telechelic DNA to link oil droplets directly at molecular scale to further improve the viscoelastic properties of emulsions.Materials and Methods:A stable emulsion with 70％oil fraction was prepared by adding 40 mg/mL of whey protein isolate and peach gum polysaccharide complex(WPI-PGP complex).The addition of telechelic DNA(0.125-0.375 µmol/L)made the emulsion change from a cream-like state to a self-supporting gel.Results:Rheological experiments confirmed that the telechelic DNA could improve the yield stress,storage modulus/loss modulus(G'/G")and apparent viscosity of the emulsion gel in a concentration-dependent manner(0-0.375 µmoVL).Inverted fluorescence images clearly showed the interdroplet network of the emulsion gel linked by telechelic DNA.For 3D printing,the emulsion gel with a higher concentration(0.375 μmol/L)of telechelic DNA displayed better surface quality and dimensional resolution,indicating improved printability.Conclusion:This study successfully designed a novel telechelic DNA-bridged emulsion gel,which showed great potential as edible ink for 3D printing.
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    The interaction mechanism between zein and folic acid in alkaline aqueous solutions:an experimental and molecular simulation study

    Jun ZhuYiling YaoYuying LiaoLala Cai...
    166-178页
    查看更多>>摘要：Objectives:Folic acid is a vitamin that is not highly soluble in water and is sensitive to the environment.Therefore,it is important to find suit-able carriers.This study aimed to exemplify the interaction of folic acid with zein in alkaline aqueous solutions and shed light on how zein can be used as a carrier for folic acid.Materials and Methods:Zein and folic acid were separately dissolved in NaOH solutions with a PH of 11.5.Zein solution and folic acid solution were combined in specific ratios.Various methods including multi-spectroscopy,dynamic light scattering,and electron microscopy combining with molecular dynamics simulations were used to study the interaction mechanism between zein and folic acid in alkaline aqueous solutions.Results:Fluorescence spectroscopy showed that the quenching of zein by folic acid was mainly static,and the main driving force behind this interaction was van der Waals forces and hydrogen bonds.The formation of zein-folic acid complexes was confirmed by ultraviolet-visible spectroscopy,Fourier transform infrared spectroscopy,and circular dichroism spectroscopy.The results also showed that the structure of zein changed when it interacted with folic acid.Dynamic light scattering analysis revealed that the addition of folic acid caused proteins to aggregate.The aggregates of the complexes had an irregular shape and were large,as observed by scanning electron microscopy and transmission elec-tron microscopy.Molecular simulation was used to further investigate the interaction mechanisms.According to these findings,the folic acid molecule interacted with zein in a shallow recess near the protein surface.The dominant forces at play in the zein-folic acid interaction were van der Waals forces and electrostatic forces,including hydrogen bonding.Conclusion:The zein alkali-soluble system is very suitable for folic acid delivery.
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    • NETL
    • NSTL
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    High-sensitivity intelligent packaging films harnessing rose anthocyanins and hydrophilic silica aerogel for visual food freshness monitoring

    Wenjun WuLuman ZhengJiahao YuLiming Liu...
    179-189页
    查看更多>>摘要：The enhancement of the sensitivity for anthocyanin-based indicator films in food freshness monitoring in real time is important for application.In this study,hydrophilic silica aerogel(SiO2NA)was incorporated into corn starch(CS)/chitosan(CH)/rose anthocyanins(RACNs)-encapsulated potato amylopectin nanoparticles(APNPs)composite film to increase the sensitivity for shrimp freshness detection.The microstructure of films revealed that the gas absorption capacity was improved by amorphous SiO2NA via hydrogen interactions.The pore size(1.74-5.60 times),pore volume(3.92-5.60 times),and specific surface area(2.21-2.34 times)of films increased with the addition of SiO2NA.The sensing of NH3 and pH and the reversibility of films were also reinforced.Meanwhile,the pH-responsive films containing SiO2NA changed visibly in color from purple-red to orange-gray and finally to gray,enabling effective monitoring of shrimp freshness during storage at 4 ℃.Thus,anthocyanin-based indicator films with improved sensitivity by adding SiO2NA were successfully designed for monitoring shrimp freshness.
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    • NETL
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    Green LED irradiation promotes the quality of cabbage through delaying senescence and regulating glucosinolate metabolism

    郭峰周宏胜胡花丽张映曈...
    190-200页
    查看更多>>摘要：Light-emitting diode(LED)irradiation can affect postharvest vegetable and fruit shelf life and nutritional quality.In this study,11 kinds of glucosinolates(GLS)and 6 types of GLS breakdown products were identified in cabbage leaves.To investigate the effects of LED irradiation on quality and GLS metabolism,postharvest cabbage was irradiated with 25 pmol/(m2·s)green LED(GL)light at room temperature for 12 d.The dark condition and natural light were used as controls.GL irradiation inhibited the respiration rate and the accumulation of malondialdehyde,and maintained higher cabbage sensory score,chlorophyll content,and antioxidant enzyme activities,thus extending its postharvest shelf life.Additionally,GL irradiation promoted GLS profile accumulation,including glucoraphanin,glucoiberin,gluconapin,gluconasturtiin,glucobrassicin,and sinigrin,which could have resulted from upregulated expression of the transcription factors MYB28and MYB51,and the key genes involved in GLS biosynthesis(CYP83A1,CYP83B1,ST5b,and UGT74B1).Furthermore,GLS degradation was promoted by this treatment during the late stage of shelf life,as higher myrosinase activity and relative MYRand ESM expression levels were found in treated cabbage leaves,resulting in higher isothiocyanate(ITC)and indole-3-carbinol contents after day 6.Therefore,GL irradiation was beneficial for cabbage preservation.
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    Genome-wide identification of the histone acetyltransferase gene family in Citrus clementina and its potential roles in citrate metabolism

    Xiahui LinYanna ShiShengchao LiuXincheng Liu...
    201-211页
    查看更多>>摘要：Histone acetyltransferase(HAT)catalyzes histone acetylation and is involved in plant growth and development and stress responses.Here,37 CitHATgenes were identified in Citrus clementina.Their physicochemical properties,chromosomal location,gene structure,conserved domain and motif,and cis-acting elements were characterized.CitHATs were classified into four subfamilies based on protein sequence homology,which was strongly supported by gene structure,conserved domain,and motif analysis.The cis-acting elements in gene promoter regions were predicted to be associated with the regulation of plant growth,stress resistance,and response to hormones.Phenotypic and transcriptomic analyses of citrus callus with mock treatment and HAT inhibitor treatment revealed that increased citric acid content in inhibitor treatment may be attributed to differ-ential expression of CitPEPCK2 and CitGS2,which may be due to the hypo-acetylation of histone H3.The expression patterns of CitHATs in citrus fruit development stages showed that CitHAG11 and CitHAG28 exhibited a negative correlation with citric acid content.Our study associates the potential function of histone acetyltransferases in citrate metabolism and extends the molecular mechanism of citrate metabolism in fruits.
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    • NETL
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    Effect of cinnamaldehyde on Rhizopus stolonifer and on the conservation of sweet potato

    陈晨薛智超Penta PristijonoJohn B.Golding...
    212-225页
    查看更多>>摘要：Objectives:Rhizopus stolonifer,which causes soft rot of sweet potato,has resulted in substantial loss of economy postharvest.Materials and Methods:The antifungal effect and mechanism of cinnamaldehyde(CIN)against R.stolonifer were explored by biochemical analysis and RNA-sequencing in this research,and the edible quality of CIN-treated sweet potato was evaluated.Results:The results showed that CIN inhibited the growth of R.stoloniferand reduced the incidence of soft rot in sweet potato at a minimum inhibitory concentration of 1000 μL/L.The damage of the cell walls of R.stolonifer by CIN was associated with the upregulation of CHT1.Cytosolic leakage and malondialdehyde content increased following CIN-treatment,which was correlated with increased reactive oxygen spe-cies levels and decreased catalase activity.Upregulation of ERG genes and oxidative stress-related genes following CIN treatment was associ-ated with impaired cell membrane integrity.The mitochondrial structure of R.stolonifer was damaged by CIN treatment resulting in decreased respiration rates and ATP production,which was correlated with lower expression levels of IDH1,COX4,and QCR7.Conclusions:Our research suggests that the inhibition of CIN on R.stolonifer was related to the disruption of its normal gene expression net-work,and CIN maintained the nutritional and edible quality of sweet potato.This study provides valuable insights into the antifungal mechanism of CIN on R.stolonifer and the maintenance of root quality,which contributes to the efficient use of CIN for controlling soft rot in sweet potato.
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