查看更多>>摘要：Human umbilical cord mesenchymal stem cells (HUC-MSCs) are pluripotent and functional in many biological processes,by which releasing secretary factors to promote the self-repairing of damaged tis-sue or developing into functional cell at local organ.However,there is a high risk that oxidative stress would reduce the pluripotency and factor-secretion during the preparation and transplantation.There-fore,reducing oxidative stress is expected to improve the efficacy of HUC-MSCs therapy.Zinc (Zn) is an essential trace element which involves in the resistance of oxidative stress.To investigate Zn-regulated signaling pathways,we have profiled the gene expression at transcriptome level in primary HUC-MSCs treated with zinc sulfate,followed with GO and KEGG gene enrichment analysis.Zn treatment im-proved signal pathways for mineral absorption,cell growth,and cell death.Zn deficiency was mim-icked by TPEN administration,which suppressed cell proliferation and reduced the expression of HUC-MSCs surface stem cell markers CD73,CD90 and CD105 by flow cytometry.Nuclear factor erythrocyte 2 related factor 2 (Nrf2) plays an important role in antioxidant biological processes.In vitro treatment of Zn significantly increased Nrf2 and Sirt3 expression at gene level and protein level respectively.Zn supplementation inhibited TPEN-induced failure of cell survival and reversed the reduction of Nrf2 and Sirt3 expression,which further reduced the production of ROS.Zn successfully presented its anti-oxidation effect by activating Nrf2/Sirt3 signaling pathway in HUC-MSCs.Zn supplementation may im-prove the efficacy of HUC-MSCs therapy with reduced oxidative stress.
查看更多>>摘要：Human umbilical cord mesenchymal stem cells (HUC-MSCs) are pluripotent and functional in many biological processes,by which releasing secretary factors to promote the self-repairing of damaged tis-sue or developing into functional cell at local organ.However,there is a high risk that oxidative stress would reduce the pluripotency and factor-secretion during the preparation and transplantation.There-fore,reducing oxidative stress is expected to improve the efficacy of HUC-MSCs therapy.Zinc (Zn) is an essential trace element which involves in the resistance of oxidative stress.To investigate Zn-regulated signaling pathways,we have profiled the gene expression at transcriptome level in primary HUC-MSCs treated with zinc sulfate,followed with GO and KEGG gene enrichment analysis.Zn treatment im-proved signal pathways for mineral absorption,cell growth,and cell death.Zn deficiency was mim-icked by TPEN administration,which suppressed cell proliferation and reduced the expression of HUC-MSCs surface stem cell markers CD73,CD90 and CD105 by flow cytometry.Nuclear factor erythrocyte 2 related factor 2 (Nrf2) plays an important role in antioxidant biological processes.In vitro treatment of Zn significantly increased Nrf2 and Sirt3 expression at gene level and protein level respectively.Zn supplementation inhibited TPEN-induced failure of cell survival and reversed the reduction of Nrf2 and Sirt3 expression,which further reduced the production of ROS.Zn successfully presented its anti-oxidation effect by activating Nrf2/Sirt3 signaling pathway in HUC-MSCs.Zn supplementation may im-prove the efficacy of HUC-MSCs therapy with reduced oxidative stress.
查看更多>>摘要：The frequency-dependent signal to noise ratio of cryo-electron microscopy data varies dramatically with the frequency and with the type of the data.During different steps of data processing,data with distinct SNR are used for calculations.Thus,specific weighting function based on the particular SNR should be designed to optimize the corresponding calculation.Here,we deduced these weighting func-tions by maximizing the signal to noise ratio of cross correlated coefficients.Some of our weighting functions for refinement resemble that used in the existing software packages.However,weighting functions we deduced for motion correction,particle picking and the refinement with overlapping densities differ from those employed by existing programs.Our new weighting functions may improve the calculation in these steps.
查看更多>>摘要：The frequency-dependent signal to noise ratio of cryo-electron microscopy data varies dramatically with the frequency and with the type of the data.During different steps of data processing,data with distinct SNR are used for calculations.Thus,specific weighting function based on the particular SNR should be designed to optimize the corresponding calculation.Here,we deduced these weighting func-tions by maximizing the signal to noise ratio of cross correlated coefficients.Some of our weighting functions for refinement resemble that used in the existing software packages.However,weighting functions we deduced for motion correction,particle picking and the refinement with overlapping densities differ from those employed by existing programs.Our new weighting functions may improve the calculation in these steps.
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