查看更多>>摘要：AIM:To determine the pattern of secreted mucin expression in Helicobacter pylori (H.pylori)-related,nonsteroidal anti-inflammatory drug (NSAID)-related and idiopathic gastric ulcers.METHODS:We randomly selected 92 patients with H.pylori-associated (n =30),NSAID-associated (n =18),combined H.pylori and NSAID-associated gastric ulcers (n =24),and patients with idiopathic gastric ulcers (n =20).Immunohistochemistry for T-cell CD4/CD8,and for mucin 5AC (MUC5AC) and mucin 6 (MUC6),was performed on sections of the mucosa from the ulcer margin.Inflammation score was assessed according to the Sydney system.RESULTS:MUC5AC was expressed on the surface epithelium (98.9％) and neck glands (98.9％) with minimal expression in the deep glands (6.5％).MUC6 was strongly expressed in the deep glands (97.8％),variable in the neck glands (19.6％) and absent in the surface epithelium (0％).The pattern of mucin expression in idiopathic ulcer margins was not different from the expression in ulcers associated with H.pylori,NSAIDs,or combined H.pylori and NSAIDs.CD4/CD8 ratio was higher in H.pylori-positive patients (P =0.009).Idiopathic ulcers are associated with hospitalized patients and have higher bleeding and mortality rates.CONCLUSION:Idiopathic ulcers have a unique clinical profile.Gastric mucin expression in idiopathic gastric ulcers is unchanged compared with H.pylori and/or NSAID-associated ulcers.
查看更多>>摘要：AIM:To investigate optimal timing for therapeutic efficacy of entecavir for acute-on-chronic hepatitis B liver failure (ACLF-HBV) in hepatitis B e antigen (HBeAg)-negative patients.METHODS:A total of 109 inpatients with ACLF-HBV were recruited from the Department of Infectious Diseases of the Third Affiliated Hospital,Sun Yat-sen University from October 2007 to October 2010.Entecavir 0.5 mg/d was added to each patient's comprehensive therapeutic regimen.Patients were divided into three groups according to model for end-stage liver disease (MELD) score:high (≥ 30,20 males and 4 females,mean age 47.8 ± 13.5 years); intermediate (22-30,49 males and 5 females,45.9 ± 12.4 years); and low (≤22,28 males and 3 females,43.4 ± 9.4 years).Statistical analysis were performed using SPSS 11.0 software.Data with normal distribution were expressed as mean ± SD and comparisons were made with Student's t tests.A value of P ＜ 0.05 was considered statistically significant.Viral loads were related exponentially and logarithmic data were used for analysis.RESULTS:For 24 patients with MELD score ≥ 30,treatment lasted 17.2 ± 16.5 d.Scores before and after treatment were significantly different (35.97 ± 4.87 and 40.48 ± 8.17,respectively,t =-2.762,P =0.011); HBV DNA load was reduced (4.882 ± 1.847 copies log10/mL to 3.685 ± 1.436 copies log10/mL); and mortality rate was 95.83％ (23/24).Of 54 patients with scores of 22-30,treatment lasted for 54.0 ± 43.2 d; scores before and after treatment were 25.87 ± 2.33 and 25.82 ± 13.92,respectively (t =-0.030,P =0.976); HBV DNA load decreased from 6.308 ± 1.607 to 3.473 ± 2.097 copies log10/mL; and mortality was 51.85％ (28/54).Of 31 patients with scores ≤ 22,treatment lasted for 66.1 ± 41.9 d; scores before and after treatment were 18.88 ± 2.44 and 12.39 ± 7.80,respectively,(t =4.860,P =0.000);HBV DNA load decreased from 5.841 ± 1.734 to 2.657 ± 1.154 copies log10/mL; and mortality was 3.23％ (1/31).CONCLUSION:For HBeAg-negative patients with ACLF-HBV,when entecavir was added to comprehensive therapy,a MELD score ≥ 30 predicted very poor prognosis due to fatal liver failure.
查看更多>>摘要：AIM:To investigate whether the side population (SP)cells possess cancer stem cell-like characteristics in vitro and the role of SP cells in tumorigenic process in gastric cancer.METHODS:We analyzed the presence of SP cells in different human gastric carcinoma cell lines,and then isolated and identified the SP cells from the KATO Ⅲ human gastric cancer cell line by flow cytometry.The clonogenic ability and self-renewal were evaluated by clone and sphere formation assays.The related genes were determined by reverse transcription polymerase chain reaction.To compare tumorigenic ability,SP and non-side population (NSP) cells from the KATO Ⅲ human gastric cancer cell line were subcutaneously injected into nude mice.RESULTS:SP cells from the total population accounted for 0.57％ in KATO Ⅲ,1.04％ in Hs-746T,and 0.02％ in AGS (CRL-1739).SP cells could grow clonally and have self-renewal capability in conditioned media.The expression of ABCG2,MDRI,Bmi-1 and Oct-4 was different between SP and NSP cells.However,there was no apparent difference between SP and NSP cells when they were injected into nude mice.CONCLUSION:SP cells have some cancer stem celllike characteristics in vitro and can be used for studying the tumorigenic process in gastric cancer.
查看更多>>摘要：AIM:To investigate whether the 7-difluoromethoxyl-5,4'-di-n-octylgenistein (DFOG),a novel synthetic genistein analogue,affects the growth of gastric cancer cells and its mechanisms.METHODS:A series of genistein analogues were prepared by difluoromethylation and alkylation,and human gastric cancer cell lines AGS and SGC-7901 cultured in vitro were treated with various concentrations of genistein and genistein analogues.The cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT) assay.The cells were incubated by DFOG at different concentrations.The growth inhibitory effects were evaluated using MTT and clonogenic assay.The distribution of the phase in cell cycle was analyzed using flow cytometric analysis with propidium iodide staining.The expression of the transcription factor forkhead box M1 (FOXM1) was analyzed by reverse transcription-polymerase chain reaction and Western blotting.The expression levels of CDK1,Cdc25B,cyclin B and p27KIP1 protein were detected u(s)ing Western blotting.RESULTS:Nine of the genistein analogues had more effective antitumor activity than genistein.Among the tested analogues,DFOG possessed the strongest activity against AGS and SGC-7901 cells in vitro.DFOG significantly inhibited the cell viability and colony formation of AGS and SGC-7901 cells.Moreover,DFOG efficaciously arrested the cell cycle in G2/M phase.DFOG decreased the expression of FOXM1 and its downstream genes,such as CDK1,Cdc25B,cyclin B,and increased p27KIP1 at protein levels.Knockdown of FOXM1 by small interfering RNA before DFOG treatment resulted in enhanced cell growth inhibition in AGS cells.Up-regulation of FOXM1 by cDNA transfection attenuated DFOG-induced cell growth inhibition in AGS cells.CONCLUSION:DFOG inhibits the growth of human gastric cancer cells by down-regulating the FOXM1 expression.
查看更多>>摘要：Gastrointestinal complications caused by herpes zoster are extremely rare.Here,we described a case of abdominal distention caused by herpes zoster.The patient was a 59-year-old female who suffered from unexplained paroxysmal and a burning pain on the right part of her waist and abdomen,accompanied by abdominal distention.Intestinal pseudo-obstruction was diagnosed by abdominal radiography.Distention of the right abdominal wall was still apparent after one month.In this report,we found that recovery from abdominal distention caused by herpes zoster is difficult and may require surgical intervention.
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