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世界胃肠病学杂志（英文版）
世界胃肠病学杂志（英文版）

潘伯荣

周刊

1007-9327

wjg@wjgnet.com

010-85381901-628

100025

北京市朝阳区东四环中路62号楼远洋国际中心D座903室

世界胃肠病学杂志（英文版）/Journal World Journal of GastroenterologyCSCDCSTPCDSCI
查看更多>>主要报道和刊登国内外、特别是我国消化病学者具有创造性的、有较高学术水平的基础和临床研究论文、研究快报等. 对具有中国特色的研究论文, 如食管癌、胃癌、肝癌、大肠癌、病毒性肝炎、幽门螺杆菌、中医中药、中西医结合和基于作者自己研究工作为主的综述性论文, 将优先发表. 读者对象为基础研究或临床研究的消化专业工作者。
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    Germline mutation analysis of MLH1 and MSH2 in Malaysian Lynch syndrome patients

    Mohd Nizam ZaharyGurjeet KaurMuhammad Radzi Abu HassanHarjinder Singh...
    814-820页
    查看更多>>摘要：AIM:To investigate the protein expression profile of mismatch repair (MMR) genes in suspected cases of Lynch syndrome and to characterize the associated germline mutations.METHODS:Immunohistochemical analysis of tumor samples was performed to determine the protein expression profile of MMR protein.Germline mutation screening was carried out on peripheral blood samples.The entire exon regions of MLH1 and MSH2 genes were amplified by polymerase chain reaction,screened by denaturing high performance liquid chromatography (dHPLC) and analyzed by DNA sequencing to characterize the germline mutations.RESULTS:Three out of 34 tissue samples (8.8％) and four out of 34 tissue samples (11.8％) showed loss of nuclear staining by immunohistochemistry,indicating the absence of MLH1 and MSH2 protein expression in carcinoma cells,respectively.dHPLC analysis followed by DNA sequencing showed these samples to have germline mutations of MSH2 gene.However,no deleterious mutations were identified in any of the 19 exons or coding regions of MLH1 gene,but we were able to identify MLH1 promoter polymorphism,-93G ＞A (rs1800734),in 21 out of 34 patients (61.8％).We identified one novel mutation,transversion mutation c.2005G ＞ C,which resulted in a missense mutation (Gly669Arg),a transversion mutation in exon 1,c.142G ＞ T,which resulted in a nonsense mutation (Glu48Stop)and splice-site mutation,c.2006-6T ＞ C,which was adjacent to exon 13 of MSH2 gene.CONCLUSION:Germline mutations were identified in four Malaysian Lynch syndrome patients.Immunohistochemical analysis of tumor tissue proved to be a good pre-screening test before proceeding to germline mutation analysis of DNA MMR genes.
      原文链接:
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    • 万方数据
    • 维普

    Meta-analysis of combined therapy for adult hepatitis B virus-associated glomerulonephritis

    Xiao-Yong ZhengRi-Bao WeiLi TangPing Li...
    821-832页
    查看更多>>摘要：AIM:To investigate the efficacy and safety of combined antiviral and immunosuppressant therapy in adult hepatitis B virus-associated glomerulonephritis (HBVGN) patients.METHODS:A computerized literature search was carried out in the PubMed database,Embase,the Cochrane Library,Chinese BioMedical Literature on disc,Chinese Medical Current Contents,Chinese National Knowledge Infrastructure,Wanfang and VIP (Chinese Technological Journal of Database) to collect articles between June 1980 and December 2010 on therapy with immunosuppressants,e.g.,glucorticosteroids,mycophenolate mofetil and leflunomide,combined with antivirals,e.g.,interferon,lamivudine,entecavir and adefovir dipivoxil,in adult HBV-GN patients.The primary outcomes were remission of proteinuria,clearance of HBV e-antigen,and elevation of serum albumin.The secondary outcomes were blood levels of alanine aminotransferase,serum creatinine,and HBV-DNA titer.Meta-analysis was performed using Review Manager 5.1.Fixed or random effect models were employed to combine the results after a heterogeneity test.The effects of the combined therapy were analyzed for different doses of glucorticosteroid and different types of HBV-GN.RESULTS:Twelve clinical trials with 317 patients were included.A significantly higher incidence of HBV-GN was found in male patients (relative risk =2.40,95％CI:1.98-2.93).Combined therapy reduced the proteinuria significantly with a mean difference of 4.19(95％ CI:3.86-4.53) and increased the serum albumin concentration significantly with a mean difference of -11.95 (95％ CI:-12.97-10.93) without significant alterations of liver function (mean difference:4.62,95％CI:-2.55-11.79) and renal function (mean difference:10.29,95％ CI:0.14-20.45).No significant activation of HBV-DNA replication occurred (mean difference:0.12,95％ CI:-0.37-0.62).There was no significant difference between the high dose glucorticosteroid group and the low dose glucorticosteroid group in terms of proteinuria remission (P =0.76) and between different pathological types of HBV-GN [membranous glomerulonephritis (MN) vs mesangial proliferative glomerulonephritis,P =0.68; MN vs membranoproliferative glomerulonephritis,P =0.27].CONCLUSION:Combined antiviral and immunosuppressant therapy can improve the proteinuria in HBVGN patients without altering HBV replication or damaging liver and renal functions.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    D2 dissection in laparoscopic and open gastrectomy for gastric cancer

    Ming CuiJia-Di XingWei YangYi-Yuan Ma...
    833-839页
    查看更多>>摘要：AIM:To evaluate the radicalness and safety of laparoscopic D2 dissection for gastric cancer.METHODS:Clinicopathological data from 209 patients with gastric cancer,who underwent radical gastrectomy with D2 dissection between January 2007 and February 2011,were analyzed retrospectively.Among these patients,131 patients underwent laparoscopyassisted gastrectomy (LAG) and 78 underwent open gastrectomy (OG).The parameters analyzed included operative time,blood loss,blood transfusion,morbidity,mortality,the number of harvested lymph nodes (HLNs),and pathological stage.RESULTS:There were no significant differences in sex,age,types of radical resection [radical proximal gastrectomy (PG + D2),radical distal gastrectomy (DG + D2) and radical total gastrectomy (TG + D2)],and stages between the LAG and OG groups (P ＞ 0.05).Among the two groups,127 cases (96.9％) and 76 cases (97.4％) had 15 or more HLNs,respectively.The average number of HLNs was 26.1 ± 11.4 in the LAG group and 24.2 ± 9.3 in the OG group (P =0.233).In the same type of radical resection,there were no significant differences in the number of HLNs between the two groups (PG + D2:21.7 ± 7.5 vs 22.4 4-9.3;DG + D2:25.7 ± 11.0 vs 22.3 ± 7.9; TG + D2:30.9 ± 13.4 vs 29.3 ± 10.4; P ＞ 0.05 for all comparisons).Tumor free margins were obtained in all cases.Compared with OG group,the LAG group had significantly less blood loss,but a longer operation time (P ＜ 0.001).The morbidity of the LAG group was 9.9％,which was not significantly different from the OG group (7.7％) (P=0.587).The mortality was zero in both groups.CONCLUSION:Laparoscopic D2 dissection is equivalent to OG in the number of HLNs,regardless of tumor location.Thus,this procedure can achieve the same radicalness as OG.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Expression of fibroblast activation protein in human pancreatic adenocarcinoma and its clinicopathological significance

    Min ShiDang-Hui YuYing ChenChen-Yan Zhao...
    840-846页
    查看更多>>摘要：AIM:To examine fibroblast activation protein (FAP)expression in pancreatic ductal adenocarcinoma (PDAC)and to analyze its relationship with the clinicopathology of PDAC.METHODS:FAP expression was examined in 134 PDAC specimens by immunohistochemistry,and in four pancreatic cancer cell lines (SW1990,Miapaca-2,AsPC-1 and BxPC-3) by Western blotting assay.We also analyzed the association between FAP expression in PDAC cells and the clinicopathology of PDAC patients.RESULTS:The results showed that the FAP was expressed in both stromal fibroblast cells (98/134,73.1％) and carcinoma cells (102/134,76.1％).All 4 pancreatic cancer cell lines expressed FAP protein at different levels.Protein bands corresponding to the proteolytically active 170-kDa seprase dimer and its 88-kDa seprase subunit were identified.Higher FAP expression in carcinoma cells was associated with tumor size (P ＜ 0.001),fibrotic focus (P =0.003),perineural invasion (P =0.009) and worse clinical outcome (P =0.0085).CONCLUSION:FAP is highly expressed in carcinoma cells and fibroblasts in PDAC tissues,and its expression is associated with desmoplasia and worse prognosis.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Interleukin-10-1082G/A polymorphism and acute liver graft rejection: A meta-analysis

    Fei LiuBo LiWen-Tao WangYong-Gang Wei...
    847-854页
    查看更多>>摘要：AIM:To investigate the association between interleukin (IL)-10-1082 (G/A) promoter polymorphism and acute rejection (AR) in liver transplant (LT) recipients.METHODS:Two investigators independently searched the Medline,Embase,China National Knowledge Infrastructure,and Chinese Biomedicine Databases.Summary odds ratios (ORs) and 95％ CIs for IL-10-1082 G/A polymorphism and AR were calculated in a fixedand a random-effects model as appropriate.RESULTS:This meta-analysis included seven casecontrol studies,which comprised 652 cases of LT recipients in which 241 cases developed AR and 411 cases did not develop AR.Overall,the variant A allele was not associated with AR risk when compared with the wild-type G allele (OR =0.94,95％ CI:0.64-1.39).Moreover,similar results were observed when the AA genotype was compared with the AG/GG genotype (OR=1.05,95％ CI:0.55-2.02).When stratifying for ethnicity,no significant association was observed among either Caucasians or Asians.Because only one study was performed in Asian patients,the result of subgroup analysis by ethnicity would not be reliable for Asians.Limiting the analysis to the studies with controls in the Hardy-Weinberg equilibrium,the results were persistent and robust.No publication bias was found in the present study.CONCLUSION:This meta-analysis suggests that IL-10-1082 G/A polymorphism may be not associated with AR risk in LT recipients among Caucasians.
      原文链接:
    • NETL
    • NSTL
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    Amplifications of NCOA3 gene in colorectal cancers in a Chinese population

    Zhi LiZheng-Yu FangYi DingWan-Tong Yao...
    855-860页
    查看更多>>摘要：AIM:To investigate the copy number variation of NAC03 gene in colorectal cancer (CRC) and its correlation with tumor progression.METHODS:A total of 142 samples of case-matched CRC tissues and adjacent normal tissues were obtained from patients undergoing bowel resection.Quantitative real-time polymerase chain reaction method was used to investigate the copy number variations of NCOA3 as well as gene expression in the collected tissues.RESULTS:Copy number gains of NCOA3 were detected in 39 CRC samples (27.5％) and were correlated with tumor progression (x2 =6.42,P =0.0112).Moreover,there was a positive correlation between copy number gain and mRNA over-expression of NCOA3 in CRCs (P =0.0023).Expression level of NCOA3 mRNA was also enhanced in the CRC samples with unaltered copy numbers (3.85 ± 1.23 vs 2.71 ± 0.64,P ＜ 0.01).CONCLUSION:Sporadic colorectal cancers exhibit different mechanisms of NCOA3 regulation.
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    • NETL
    • NSTL
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    A case of Cowden syndrome diagnosed from multiple gastric polyposis

    Minsu HaJun Won ChungKi Baik HahmYoon Jae Kim...
    861-864页
    查看更多>>摘要：Cowden syndrome is a rare autosomal dominant disorder that is characterized by multiple hamartomas in a variety of tissues and this is associated with germline mutations in the phosphatase and tensin homologue (PTEN) gene,which is the tumor suppressor gene located on chromosome 10q23.3.It is characterized by multiple hamartomatous neoplasms of the skin,oral mucosa,gastrointestinal (GI) tract,bones,central nervous system,eyes,and genitourinary tract.Cowden syndrome does not have increased risk of GI malignancy; however,it has an increased risk of breast,thyroid and endometrial cancer development.Here the authors report a rare case of Cowden syndrome incidentally diagnosed from multiple gastric polyposis.A 29-year-old woman presented with multiple gastric polyps.The laboratory results were normal except for mild anemia,with a hemoglobin level of 11.9 g/dL.Esophagogastroduodenoscopy revealed multiple gastric,duodenal polyps and esophageal acanthosis.Colonoscopy revealed possible hamartomatous polyps in the rectum.Under the suspicion of Cowden syndrome,sonography of the thyroid and breasts was carried out,which revealed multiple thyroid masses.Subsequent fine-needle aspiration biopsy revealed the presence of clusters of follicular epithelial cells,and due to the possibility of malignancy,the patient underwent total thyroidectomy.The pathology was reported as invasive follicular carcinoma.A gene study by direct sequencing showed the presence of a PTEN mutation (c.633C ＞ A/p.Cys211*).
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    • 维普

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      原文链接:
    • NETL
    • NSTL
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