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海洋湖沼学报（英文版）
中科院海洋所 中国海洋湖沼学会
海洋湖沼学报（英文版）

中科院海洋所 中国海洋湖沼学会

曾呈奎

双月刊

2096-5508

jol@qdio.ac.cn

0532-82898754

266073

山东省青岛市市南区福山路32号

海洋湖沼学报（英文版）/Journal Journal of Oceanology and LimnologyCSCDCSTPCD北大核心SCI
查看更多>>《中国海洋湖沼学报（英文版）》是由中国海洋湖沼学会主办、中国科学院海洋研究所承办的一级学报类学术期刊，1983年创刊，现为双月刊。本刊主要刊登内容为海洋湖沼科学及相关的生物、水产养殖、物理、气象、化学、地质、水文、地貌、工程、环保、遥感、仪器等方面的国内外最新研究成果，包括研究论文，简报，综述等。自2018年起《中国海洋湖沼学报（英文版）》更名为《海洋湖沼学报（英文）》。
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    Detoxification strategy of Microcystis aeruginosa to the toxicity of Cd(Ⅱ):role of EPS in alleviating toxicity

    Xingye HANFangyuan LIUYibo ZHANGKai CHENG...
    802-815页
    查看更多>>摘要：Although many studies have found that cadmium(Cd)can be toxic to microalgae,only a few reports focused on the role of extracellular polymeric substances(EPS)in Cd(Ⅱ)detoxification.The biochemical and physiological endpoints of Microcystis aeruginosa,including the composition and functional groups of soluble EPS(SL-EPS),loosely bound EPS(LB-EPS),and tightly bound EPS(TB-EPS),were detected to elucidate the toxicity and detoxification mechanisms of Cd(Ⅱ)for cyanobacteria.Toxicological and physiological assays on M.aeruginosa showed that the 0.25-mg/L Cd(Ⅱ)resulted in a larger inhibition on growth and Fv/Fm.Nevertheless,Cd(Ⅱ)significantly induced much higher contents of superoxide dismutase(SOD),intracellular microcystin LR(MC-LR),extracellular MC-LR,and EPS.Scanning electron microscopy with energy dispersive X-ray spectroscopy confirmed that Cd(Ⅱ)was absorbed into the EPS layer.Fourier transform infrared spectrum analysis revealed that the functional groups bound with Cd(Ⅱ)of algae biomass,SL-EPS,LB-EPS,and TB-EPS were somewhat different.The C=O/C=N groups of δ-lactam or protein were their prominent functional groups,suggesting that amide or proteins in the EPS played a key role in the adsorption in Cd(Ⅱ).The concentration of 0.25 mg/L of Cd(Ⅱ)may change the chemical structure of EPS by altering the production of protein-like substances containing tryptophan.This study indicated that M.aeruginosa could detoxify Cd(Ⅱ)stress via induction of antioxidant capacity(higher SOD activity and MC synthesis),EPS production,and modification in chemical structure of EPS.
      原文链接:
    • 国家科技期刊平台
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    Analysis on the causes of massive stranding of Yellow Sea green tide on Lianyungang and Rizhao coasts in 2022

    Lingjuan WUJuan HUANGYi DINGGuiyan LIU...
    816-830页
    查看更多>>摘要：In 2022,Yellow Sea green tide caused by Ulva prolifera reached a historic minimum and the coastal areas of Shandong Peninsula were less affected.However,the largest amount of seaweed biomass has been washed ashore on Lianyungang and Rizhao coasts since 2015.We studied the development pattern of Yellow Sea green tide in 2022,and analyzed the key environmental factors on the growth and drifting,then discussed the possible reasons that resulted in the massive stranding of green tide biomass in Lianyungang and Rizhao.Results show under the combined influence of the east to southeast winds and currents with shoreward anomalies,green tide drifted to the coastal waters between Shandong and Jiangsu provinces and the distribution areas located westward compared with previous years(2008-2021).Floating U.prolifera rafts from the coastal waters of Binhai and Sheyang drifted continuously into the coastal waters of Lianyungang and Rizhao,providing important supplements for Yellow Sea green tide.Because green tide in 2022 distributed close to the coastal waters,the abundant nutrients might support their continuous high growth rate.In addition,the amount of rainfall around Shandong Peninsula from late June to early July were significantly higher than in previous years,which might promote the development of green tide to some extent.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Morphological and molecular analyses of a Philine kinglipini outbreak in summer of 2022 in Jiaozhou Bay,China

    Zelin DUANYanan YUJing WANGJunlong ZHANG...
    831-848页
    查看更多>>摘要：A sudden and unprecedented outbreak of molluscan Philine kinglipini occurred in summer 2022 in Jiaozhou Bay,Shandong,China,causing substantial damage to local mariculture industry of the Manila clam Ruditapes philippinarum.Although P.kinglipini has been found in many coastal regions of China,the molecular research of P.kinglipini has not been extensively studied,making it difficult to accurately identify and track P.kinglipini samples in field using molecular methods.Samples were collected during the outbreak and their morphological features and molecular sequences were analyzed.Results show that the causative species of the outbreak was P.kinglipini.The mitochondrial genome(mtDNA)of P.kinglipini was constructed for the first time,based on which phylogenetic analysis of the mtDNAs of P.kinglipini and related species in the order Cephalaspidea was carried out.As revealed by metabarcoding analysis of 18S rDNA V4,the seasonal change of P.kinglipini and closely related Philine species was striking with peaks between April and August.Therefore,metabarcoding analysis is applicable tool for monitoring the bloom development of P.kinglipini and related species.This study generated for the first time essential molecular marker sequences and mtDNA of P.kinglipini,which provided a reference for future characterization and monitoring of its outbreaks and for phylogenetic analysis of Philine species.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Seasonal changes in gut microbiota of sea cucumber over natural aestivation cycle

    Ye ZHAOHan WANGHaona WANGLijie ZHANG...
    849-864页
    查看更多>>摘要：Sea cucumber Apostichopus japonicus is an ideal model organism for marine invertebrate aestivation;it annually enters a"sleeping state"for nearly 3 months when ambient water temperature range is 25-30 ℃.The natural fasting is accompanied by remodeling the intestinal biota and function,which is a part of host biology and could affect the gut microbiota.We investigatesd the impact of annual aestivation on gut microbiota using high-throughput sequencing of 16S rRNA amplicons.Results reveal a notable alteration in the composition of gut bacteria during aestivation during which various indigenous families and genera that exhibit a preference for dietary glycans(e.g.,family Rhodobacteraceae and Flavobacteriaceae)decreased,while the taxa capable of utilizing substrates derived from the host(e.g.,genus Akkermansia and Prevotella)increased,and so did certain opportunistic pathogenic bacteria.Moreover,the investigation delved into the gut morphology and immunity response of A.japonicus and reveal that the intestine of A.japonicus undergoes substantial atrophy and degeneration during aestivation.However,there was an observed augmentation in the levels of acid and neutral mucin within mucous cells,as well as an enhanced immune defense response(as evidenced by increased gene expression of AjTLR3,LITAF,Ajp105,and LYZ).These results imply that the composition of sea cucumber gut microbiota differed between aestivation and active periods,which potentially affects the intestinal functions of the host and the symbiotic relationship between host and its microbiota over the active-aestivation cycle.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Transcriptome profiling and RXR gene family identification reveals the molecular mechanism of rapid aging after spawning of cuttlefish Sepiella japonica

    Zhenyu DONGJiemei ZHAOFeng GUOShuangrui LIN...
    865-880页
    查看更多>>摘要：Sepiella japonica is a worldwide marine cuttlefish species of high economic value.S.japonica routinely modifying behaviors in reproductive life,such as rapid aging until death after spawning,has been recognized in artificial breeding.However,reproductive behavior at the level of genes is rarely reported,thus,the research on the genetic basis of behavior,reproduction,and artificial breeding was limited.We applied RNA-seq in different stages of reproduction to investigate the reason of rapid aging after spawning,pre-maturity,pre-spawning after maturity,and post-spawning.The retinoid X receptor(RXR)gene family in S.japonica was identified,and 1 343-1 452 differentially expressed genes(DEGs)in all 3 stages of reproductive life were identified from pairwise mRNA comparisons.Furthermore,through the GO term and KEGG analysis,S.japonica could handle neuronal development and network formation before maturity and have a functional degradation of neural communication,signal transduction,vision,and gene expression after spawning.Eight SjRXRas have been identified and they played different roles in growth development or reproduction.Therefore,the regulation of several channels and receptors is the intrinsic molecular mechanism of rapid aging after spawning in S.japonica.This study revealed the survival strategy and provided fundamental data on the level of genes for understanding the reproductive behavior and the reproduction of S.japonica.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Reproductive biology of Hemitripterus villosus in the Bohai Strait,China

    Ping SUNSong QINZhengyi LIULongchuan ZHUANG...
    881-892页
    查看更多>>摘要：To understand the reproductive biology of Hemitripterus villosus in the Bohai Strait,and fill the key knowledge gap in verifying reproductive seasonality and documenting gonadotropin dependent reproductive cycle of this species,a year-round detail study was conducted for the first time.In total,263 individuals were randomly sampled off the coasts of the Bohai Strait.Results show that and the overall female-to-male ratio(1.35∶1)did not differ significantly from the 1∶1 sex ratio.The length and weight relationship for females and males were regressed,depicting an isometric growth for both sexes.Four reproductive phases of the complete gonadotropin dependent reproductive cycle in both sexes were identified.The monthly variations in the gonadosomatic and hepatosomatic indexes for both sexes further indicated that this species spawns a single batch of eggs per year and the peak spawning period is from October to December.The average diameter of eggs ranged from 2.10 to 3.57 mm.The length at first sexual maturity was 224.87 mm for females and 241.26 mm for males.Significant positive relationships between absolute fecundity and fish size,and also gonadal weight were observed.H.villosus from the Bohai Strait exhibited smaller body size,smaller egg diameter,lower gonadosomatic index,lower absolute fecundity,and higher population extinction risk than the other populations'studied previously.Our findings contribute to understanding the status of this isolated westernmost population,and we emphasize that more efficient protection measures for the species and control measures are urgently required to improve the coastal environment in the Bohai Strait.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber

    Yanan LIZongfu CHENHaibin ZHANGRuoyu LIU...
    893-904页
    查看更多>>摘要：Manganese superoxide dismutase(MnSOD)is an antioxidant that exists in mitochondria and can effectively remove superoxide anions in mitochondria.In a dark,high-pressure,and low-temperature deep-sea environment,MnSOD is essential for the survival of sea cucumbers.Six MnSODs were identified from the transcriptomes of deep and shallow-sea sea cucumbers.To explore their environmental adaptation mechanism,we conducted environmental selection pressure analysis through the branching site model of PAML software.We obtained night positive selection sites,and two of them were significant(97F→H,134K→V):97F→H located in a highly conservative characteristic sequence,and its polarity change might have a great impact on the function of MnSOD;134K→V had a change in piezophilic ability,which might help MnSOD adapt to the environment of high hydrostatic pressure in the deepsea.To further study the effect of these two positive selection sites on MnSOD,we predicted the point mutations of F97H and K134V on shallow-sea sea cucumber by using MAESTROweb and PyMOL.Results show that 97F→H,134K→V might improve MnSOD's efficiency of scavenging superoxide anion and its ability to resist high hydrostatic pressure by moderately reducing its stability.The above results indicated that MnSODs of deep-sea sea cucumber adapted to deep-sea environments through their amino acid changes in polarity,piezophilic behavior,and local stability.This study revealed the correlation between MnSOD and extreme environment,and will help improve our understanding of the organism's adaptation mechanisms in deep sea.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
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    Bioactive chemical constituents from the marine-derived fungus Cladosporium sp.DLT-5

    Luting DAIQingyi XIEJiaocen GUOQingyun MA...
    905-914页
    查看更多>>摘要：A new isochromanone,cladosporinisochromanone(1),accompanied by 15 known compounds(2-16)were obtained from secondary metabolites produced by marine-derived fungus Cladosporium sp.DLT-5.NMR and HRESIMS spectra elucidation determined the planar structure of 1.Subsequent electronic circular dichroism(ECD)experiment assigned the absolute configuration of 1.Compounds 1,2,4-6,and 10 displayed different degrees of neuroprotective activities on human neuroblastoma cells SH-SY5Y.Five compounds(1,3-5,and 13)emerged resistance to protein tyrosine phosphatase lB(PTP1B),further kinetic analysis and molecular docking study indicated that the most potent compound 13(IC50 value of 10.74±0.61 μmol/L)was found as a noncompetitive inhibitor for PTP1B.Surface plasmon resonance(SPR)and molecular docking studies also demonstrated the interaction between compound 12 and Niemann-Pick Cl Like 1(NPC1L1),which has been identified as significant therapeutic target for hypercholesteremia.In addition,compounds 3,6,and 14 showed attractive inhibitory activity against the phytopathogenic fungi:Colletotrichum capsici.Therefore,library of Cladosporium metabolites is enriched and new active uses of known compounds are explored.
      原文链接:
    • 国家科技期刊平台
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    A preliminary study on life history of marine red alga Stylonema alsidii(Zandardini)Drew f.ramosum Zheng et Li

    Bingxin HUANGYao ZHANGYue CHUYouxuan GUO...
    915-922页
    查看更多>>摘要：Stylonema alsidii(Zandardini)Drew f.ramosum Zheng et Li is a common species in the genus Stylonema,but there are limited reports on morphological taxonomy and a lack of studies on life history and the regulation of early development.The early development,discoid formation,and erect thallus growth of Stylonema alsidii f.ramosum were observed under laboratory conditions.In addition,the influence of main environmnental factors such as temperature and light intensity on its development was briefly studied,and various stages of its life history were observed.The result shows:(1)there are two reproductive modes:asexual reproduction and sexual reproduction;(2)the morphology of this species varies in different degrees under laboratory culture conditions;(3)sexual reproduction and asexual reproduction can coexist under suitable conditions.Five pathways of life history were inferred from experimental phenomena.Mature sporophytes form mature gametophytes by producing initially isolated or initially attached monospore,and mature gametophytes form mature sporophytes by fertilization of eggs and sperms.The sexual and asexual reproduction processes of Stylonema alsidii f.ramosum were reported for the first time,and this is also the first report on sexual reproduction in the genus Stylonema.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Morphological comparison and molecular marker screening of three Skeletonema species found in Changjiang(Yangtze)River Basin

    Jingwen HUYuxin YIZhengxin YANGJohn Patrick KOCIOLEK...
    923-942页
    查看更多>>摘要：In our recent investigations of diatom diversity,we studied three species,namely,Skeletonema costatum,Skeletonema subsalsum,and Skeletonema potamos.Although they have been found frequently in Changjiang(Yangtze)River Basin,their morphological and molecular identification is difficult in taxonomy.Therefore,to integrate morphological and molecular biological approaches,we compared systematically their morphological characters and performed phylogenetic analysis.Twelve strains of Skeletonema were collected and isolated from Shanghai and Jiangsu,China,and their morphological characteristics were examined by light microscopy(LM)and the scanning electron microscopy(SEM).Based on morphological comparison,we determined that S.potamos is easy to distinguish from the other two species.The heavily silicified areolae,undulated or cleft distal ends of terminal fultoportula processes(TFPPs),absence of basal pores of fultoportula processes(FPPs),the root-like protrusions of FPPs,and no interlocking connection are the stable characteristics that can be used to identify S.potamos.However,there are only two features that can distinguish S.costatum from S.subsalsum,namely the location of terminal rimoportulae(TRPs)and the distal shape of TFPPs.In addition,we amplified and sequenced nine common genetic markers from the strains,from which 101 sequences were obtained,constructed phylogenetic trees based on the nine genes and evaluated that seven genes can be used to identify S.potamos,and revealed that S.subsalsum is the closest known relative of S.costatum,and only ATP synthetase beta-subunit gene(atpB)is able to distinguish them from each other,which strongly support that it is an effective molecular marker for Skeletonema.This work provided a theoretical basis for the taxonomic study of Skeletonema.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据