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海洋湖沼学报（英文版）
中科院海洋所 中国海洋湖沼学会
海洋湖沼学报（英文版）

中科院海洋所 中国海洋湖沼学会

曾呈奎

双月刊

2096-5508

jol@qdio.ac.cn

0532-82898754

266073

山东省青岛市市南区福山路32号

海洋湖沼学报（英文版）/Journal Journal of Oceanology and LimnologyCSCDCSTPCD北大核心SCI
查看更多>>《中国海洋湖沼学报（英文版）》是由中国海洋湖沼学会主办、中国科学院海洋研究所承办的一级学报类学术期刊，1983年创刊，现为双月刊。本刊主要刊登内容为海洋湖沼科学及相关的生物、水产养殖、物理、气象、化学、地质、水文、地貌、工程、环保、遥感、仪器等方面的国内外最新研究成果，包括研究论文，简报，综述等。自2018年起《中国海洋湖沼学报（英文版）》更名为《海洋湖沼学报（英文）》。
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    Molecular cloning and functional analysis of two calcium-associated cuticular protein genes in Neocaridina denticulata sinensis

    Xi GAOMengmeng DUANYuying SUNJiquan ZHANG...
    1659-1672页
    查看更多>>摘要：The body surface of crustaceans is covered with a sturdy shell.The growth and development of crustaceans are realized through molting.Neocaridina denticulata sinensis is a suitable candidate for crustacean scientific research.Two calcium-associated cuticular protein genes,named NdCAP-1 and NdCAP-2,were obtained from N.denticulata sinensis.Molecular docking simulated the binding effect of both proteins and calcium ions.Semi-quantitative reverse transcription PCR results show that NdCAP-1 is expressed in D2-4 stage,NdCAP-2 expressed inD24and A-B stages,and both were significantly expressed in the cephalothorax cuticle and pereiopods.Then,it was revealed that NdCAP-1 and NdCAP-2 are regulated by NdEcR-mediated 20E signaling pathways.Knockdown of NdCAP-1 and NdCAP-2 was observed to cause surface defects.The recombinant proteins(rNdCAP-1 and rNdCAP-2),obtained by prokaryotic expression,had calcium-binding and chitin-binding ability,inhibited formation of calcium carbonate precipitate.These results show that calcium-associated cuticular proteins play important roles in cuticle formation and calcification.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus

    Dongxiu XUESudong XIATengfei XINGYuqian GAO...
    1673-1682页
    查看更多>>摘要：The mechanisms underlying sex determination and differentiation have long intrigued researchers in the fields of development and evolutionary biology.The roughskin sculpin(Trachidermus fasciatus Heckel),displaying sexual dimorphism,provides an ideal model for studying the mechanisms.However,both genetic and genomic information concerning sex determination and differentiation,such as gonadal transcriptome data in roughskin sculpin,are lacking.Here,we present the first gonadal transcriptomes of roughskin sculpin and identify sex-related genes.We identified 8 531 differentially expressed genes(DEGs),among them 4 065 were upregulated in the ovary and 4 466 upregulated in the testis.Several sex-related gene ontology(GO)terms were enriched in ovary-biased genes,including"binding of sperm to zona pellucida","egg coat formation","positive regulation of acrosome reaction","cell division",and"cell cycle",while the GO terms such as"spermatogenesis","sperm axoneme assembly","cilium assembly","cilium movement",and"cilium movement involved in cell motility"were enriched in testis-biased genes.Moreover,six KEGG pathways were significantly enriched in the ovary,whereas only one was enriched in the testis.Of these DEGs,40 sex-related genes were identified,which including 26 testis-biased genes(such as Dmrtb1,Gsdf Sox9b,Wnt4b,Tcp11l2,and Efhb),and 14 ovary-biased genes(such as Cyp19a1a,Foxh1,Foxr1,Gdf3,Hsd17b12,and Igf2bp3).This gonadal transcript dataset would broaden our understanding of sex determination and differentiation mechanisms in roughskin sculpin,expand the genomic database,support future studies on sex-related gene functions,and facilitate molecular biology research into roughskin sculpin.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Stock appraisal for Atlantic tripletail(Lobotes surinamensis;Bloch,1790)in the Bay of Bengal,Bangladesh

    Suman BARUAQun LIUAhmed Fazley RABBYMd.Abdullah AL-MAMUN...
    1683-1694页
    查看更多>>摘要：The Atlantic tripletail(Lobotes surinamensis)is a high revenue-generating fish species predominantly caught by mechanized artisanal fishers community and the most available member of its family in Bangladesh.This is a ground work of fish stock assessment study in the Bay of Bengal region to explore the life history parameters and associated biomass of this species,using three length-based approaches of TropFishR,the length-based Bayesian biomass estimation(LBB),and Froese's length-based indicators(LBIs).An almost homogenous body growth pattern(b=3.07;R2=0.98)was observed in the length-weight relationship of tripletail.The life history parameters for tripletail,as determined by the von Bertalanffy Growth Function(VBGF)model,were L∞=1 13.36 cm and k=0.51/a.The length converted catch curve(LCCC)yielded an estimation of the total mortality(Z=1.77/a),with the natural mortality estimated at(M=0.53/a)and the fishing mortality estimated at(F=1.24/a).But,the ratio of mortality(F/M=0.15)by LBB captured the non-fully exploited status of biomass(B/BMSY=2.1).LBI analysis indicated that the tripletail fishery's spawning stock biomass is greater than the target and limit reference points,indicating a healthy state of biomass.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Effect of length-width ratio of rounded rectangle aquaculture tank in dual-diagonal-inlet layout on hydrodynamics

    Meng LIXiaozhong RENShupeng DUWei SUN...
    1695-1709页
    查看更多>>摘要：To adapt to the change of aquaculture workshop site,optimize the shape of aquaculture tanks and improve the utilization rate of breeding space,it is necessary to determine the appropriate length-width ratio parameters of aquaculture tanks.In this paper,computational fluid dynamics(CFD)technology is adopted to study the flow field performance of aquaculture tanks with different L/B ratios(L:the length;B:the width,of aquaculture tank)and different jet direction conditions(lengthways jet and widthways jet).A three-dimensional numerical calculation model of turbulence in rounded rectangle aquaculture tanks in dual-diagonal-inlet layout was established.Jet directions are arranged lengthways and widthways,and the water flow velocity,resistance coefficient change,vorticity,etc.are analyzed under two working conditions.Results show that the flow field performance in aquaculture tank decreases with the increase of the L/B ratio.The flow field performed well when L/B was 1.0-1.3,sharply dropped at 1.4-1.6,and poor at 1.7-1.9.The results provided a theoretical basis for the design and optimization in flow field performance of the industrialized circulating aquaculture tanks.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Isolation,identification,and evaluation of intestinal bacteria in Macrobrachium rosenbergii

    Xiuxin ZHAOJinping LUOPeimin LIUHao HUANG...
    1710-1721页
    查看更多>>摘要：Probiotics are live microbial food supplements that have been shown to have beneficial effects on animal health.Endogenous probiotic bacteria have long been used for their proposed health-promoting properties and have become a hot research topic in growth improvement in aquaculture.The endogenous probiotic bacteria from intestines of Macrobrachium rosenbergii(giant river prawn)was explored for their probiotic potential,from which 367 bacterial strains were isolated from the intestine of M.rosenbergii.After 16S rDNA sequence analysis,234 isolates were identified as Lactococcus garvieae,which accounted for 63.76％of the total number of culturable intestinal bacteria,suggesting that this bacterium was the main component of the microbiota.Furthermore,to reveal the probiotic properties of L.garvieae,this isolated bacterial strain was characterized morphologically,physiologically,and biochemically.Its enzyme production capacity,bacteriostatic activity,and resistance to acid,high temperature,and pH,were assessed.In vitro experiments showed that the L.garvieae(No.C6a2)had a fast growth rate and entered the logarithmic phase rapidly.Besides,it had characteristics of acid-production and resistance,enzyme-producing capacity,and strong antibacterial activity against pathogenic Staphylococcus aureus,Aeromonas hydrophila,and Aeromonas veronii.However,it lacked the ability to tolerate high temperature.Our results provide novel data to deepen our understanding of the intestinal bacteria structure of M.rosenbergii and valuable information for probiotic screening and the application for M.rosenbergii.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    海洋湖沼学报(英文)

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      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据