期刊,中国昆虫科学（英文版） 2024年卷6期_国家学术搜索

期刊信息/Journal information

中国昆虫科学（英文版）

主　　编：王牧牧

出版周期：双月刊

国际刊号：1672-9609

电　　话：010-82672313

邮政编码：100080

地　　址：北京海淀区北四环西路25号

中国昆虫科学（英文版）/Journal Insect ScienceCSCDCSTPCD北大核心SCI

正式出版

收录年代

The symbiont Wolbachia alleviates pesticide susceptibility in the two-spotted spider mite Tetranychus urticae through enhanced host detoxification pathways

Qing-Tong YeXue GongHuan-Huan LiuBing-Xuan Wu...

1822-1837页

查看更多>>摘要：The two-spotted spider mite(Tetranychus urticae)is one of the most well-known pesticide-resistant agricultural pests,with resistance often attributed to changes such as target-site mutations and detoxification activation.Recent studies show that pes-ticide resistance can also be influenced by symbionts,but their involvement in this pro-cess in spider mites remains uncertain.Here,we found that infection with Wolbachia,a well-known bacterial reproductive manipulator,significantly increased mite survival after exposure to the insecticides abamectin,cyflumetofen,and pyridaben.Wolbachia-infected(WI)mites showed higher expression of detoxification genes such as P450,glutathione-S-transferase(GST),ABC transporters,and carboxyl/cholinesterases.RNA interference experiments confirmed the role of the two above-mentioned detoxification genes,TuCYP392D2 and TuGSTd05,in pesticide resistance.Increased GST activities were also observed in abamectin-treated WI mites.In addition,when wild populations were treated with abamectin,WI mites generally showed better survival than uninfected mites.However,genetically homogeneous mites with different Wolbachia strains showed simi-lar survival.Finally,abamectin treatment increased Wolbachia abundance without altering the mite's bacterial community.This finding highlights the role of Wolbachia in orches-trating pesticide resistance by modulating host detoxification.By unraveling the intricate interplay between symbionts and pesticide resistance,our study lays the groundwork for pioneering strategies to combat agricultural pests.

原文链接:

万方数据
维普

Comprehensive toxicity assessment of nanodiamond on Blaps polychresta:implications and novel findings

Marwa SaadNabila SelimLamia M.El-Samad

1838-1863页

查看更多>>摘要：With the increasing development of nanomaterials,the use of nanodiamonds(NDs)has been broadly manifested in many applications.However,their high penetration into the ecosystem indubitably poses remarkable toxicological risks.This paper investi-gates the toxic effects of NDs on the darkling beetle,Blaps polychresta Forskal,1775(Coleoptera:Tenebrionidae).Survival analysis was carried out by monitoring the beetles for 30 d after the injection of four different doses of NDs.A dose of 10.0 mg NDs/g body weight,causing less than 50％mortality effect,was assigned in the analysis of the different organs of studied beetles,including testis,ovary,and midgut.Structural and ul-trastructural analyses were followed using light,TEM,and SEM microscopes.In addition,a variety of stress markers and enzyme activities were assessed using spectrophotomet-ric methods.Furthermore,cell viability and DNA damage were evaluated using cytome-try and comet assay,respectively.Compared to the control group,the NDs-treated group was exposed to various abnormalities within all the studied organs as follows.Significant disturbances in enzyme activities were accompanied by an apparent dysregulation in the antioxidant system.The flow cytometry results indicated a substantial decrease of viable cells along with a rise of apoptotic and necrotic cells.The comet assay demonstrated a highly increased level of DNA damage.Likewise,histological analyses accentuated the same findings showing remarkable deformities in the studied organs.Prominently,the re-search findings substantially contribute for the first time to evaluating the critical effects of NDs on B.polychresta,adopted as the bioindicator in this paper.

原文链接:

万方数据

A self-locking mechanism of the frog-legged beetle Sagra femorata

Le ZongZonghui SunJieliang ZhaoZhengzhong Huang...

1864-1875页

查看更多>>摘要：Insect legs play a crucial role in various modes of locomotion,including walking,jumping,swimming,and other forms of movement.The flexibility of their leg joints is critical in enabling various modes of locomotion.The frog-legged leaf beetle Sagra femorata possesses remarkably enlarged hind legs,which are considered to be a critical adaptation that enables the species to withstand external pressures.When con-fronted with external threats,S.femorata initiates a stress response by rapidly rotating its hind legs backward and upward to a specific angle,thereby potentially intimidat-ing potential assailants.Based on video analysis,we identified 4 distinct phases of the hind leg rotation process in S.femorata,which were determined by the range of rotation angles(0°-168.77°).Utilizing micro-computed tomography(micro-CT)technology,we performed a 3-dimensional(3D)reconstruction and conducted relative positioning and volumetric analysis of the metacoxa and metatrochanter of S.femorata.Our analysis re-vealed that the metacoxa-trochanter joint is a"screw-nut"structure connected by 4 mus-cles,which regulate the rotation of the legs.Further testing using a 3D-printed model of the metacoxa-trochanter joint demonstrated its possession of a self-locking mechanism capable of securing the legs in specific positions to prevent excessive rotation and disloca-tion.It can be envisioned that this self-locking mechanism holds potential for application in bio-inspired robotics.

原文链接:

万方数据
维普

Novel insights into paternity skew in a polyandrous social wasp

Sarah E.OrrNicole A.HedrickKayla A.MurrayAbhinav K.Pasupuleti...

1876-1888页

查看更多>>摘要：Females of many species are polyandrous.However,polyandry can give rise to conflict among individuals within families.We examined the level of polyandry and paternity skew in the common eastern yellowjacket wasp,Vespula maculifrons,in order to gain a greater understanding of conflict in social insects.We collected 10 colonies of V maculifrons and genotyped workers and prereproductive queens at highly variable mi-crosatellite markers to assign each to a patriline.Genotypic data revealed evidence of sig-nificant paternity skew among patrilines.In addition,we found that patrilines contributed differentially to caste production(worker vs.queen),suggesting an important role for re-productive conflict not previously discovered.We also investigated if patterns of paternity skew and mate number varied over time.However,we found no evidence of changes in levels of polyandry when compared to historical data dating back almost 40 years.Finally,we measured a suite of morphological traits in individuals from the most common and least common patrilines in each colony to test if males that showed highly skewed re-productive success also produced offspring that differed in phenotype.Our data revealed weak correlation between paternity skew and morphological phenotype of offspring sired by different males,suggesting no evidence of evolutionary tradeoffs at the level investi-gated.Overall,this study is the first to report significant paternity and caste-associated skew in V maculifrons,and to investigate the phenotypic consequences of skew in a social wasp.Our results suggest that polyandry can have important consequences on the genetic and social structure of insect societies.

原文链接:

万方数据
维普

Accessory gland protein regulates pairing process and oviposition in the subterranean termite Reticulitermes chinensis after swarming

Yutong LiuFei ZhangAli HassanXuguo Zhou...

1889-1907页

查看更多>>摘要：Swarming and pairing behaviors are significant to population dispersal of ter-mites.Tandem running is a key process in pairing behavior of dealates to find a mate.Suc-cinylation can lead to significant changes in protein structure and function,which is widely involved in metabolism and behavior regulation in many organisms.However,whether succinylation modification regulates termites'tandem running is currently unknown.In this research,we performed quantitative modified proteomics of the subterranean termite Reticulitermes chinensis Snyder before and after alate swarming.The succinylation lev-els of accessory gland protein(ACP)were significantly altered after alate swarming.We found that ACP is enriched in male accessory gland and female oocytes of termites.The acetylation and succinylation sites of ACP affected tandem running of dealates.The tran-scriptome and metabolome analyses of alates injected with ACP and its mutant proteins showed that β-alanine metabolism pathway was the major downstream pathway of ACP.Silencing the significantly differentially expressed genes in the β-alanine metabolic path-way(acyl-CoA dehydrogenase,enoyl-CoA hydratase,3-hydroxyisobutyrate dehydroge-nase,methylmalonate-semialdehyde dehydrogenase)suppressed tandem running and al-tered oviposition of paired dealates.These findings demonstrate that protein translation modification is an important regulator of tandem running behavior of termites,which im-plies that the succinylation and acetylation modification sites of ACP could be potential targets for insecticide action.Our research offers a potential approach for developing novel dispersal inhibitors against social insect pests.

原文链接:

万方数据
维普

Rainfall during the night can trigger non-migratory take-off behavior of the white-backed planthopper,Sogatella furcifera

Haibo YangYunlong FengPinhong ZhuDingxu Li...

1908-1917页

查看更多>>摘要：Take-off behavior is crucial to the overall success of insect migration.Al-though most high-altitude migratory flights commence with mass take-offs around dusk and dawn,little is known about nighttime take-off behavior.The take-off behavior of mi-gratory Sogatella furcifera was investigated in field cages from 2017 to 2019.The species showed a bimodal take-off pattern at dusk and dawn on rainless nights,with mass flight at dusk more intense than dawn flight.However,a higher frequency of take-offs during the nighttime was observed on rainy nights,resulting in the absence of dawn take-offs.Most migratory take-off individuals at dusk and dawn landed on the cage top or the walls above 150 cm,while non-migratory individuals that took off during the nighttime due to rainfall mainly landed on the cage walls below 150 cm.Furthermore,it has been observed that migratory take-off individuals possess stronger sustained flight capabilities and exhibit more immature ovaries compared with non-migratory take-offs.These findings advance our understanding of the take-off behavior of S.furcifera and thus provide a basis for the accurate prediction and management of the migratory dynamics of this pest.

原文链接:

万方数据

Competition,cooperation,and parental effects in larval aggregations formed on carrion by communally breeding beetles Necrodes littoralis(Staphylinidae:Silphinae)

Natalia LisAnna Mądra-BielewiczJędrzej WydraSzymon Matuszewski...

1918-1929页

查看更多>>摘要：Aggregations of juveniles are dominant forms of social life in some insect groups.Larval societies are shaped by competitive and cooperative interactions of the lar-vae,in parallel with parental effects.Colonies of necrophagous larvae are excellent sys-tems to study these relationships.Necrodes littoralis(Staphylinidae:Silphinae),a carrion beetle that colonizes cadavers of large vertebrates,forms massive juvenile aggregations.By spreading over carrion anal and oral exudates,the beetles form the feeding matrix,in which the heat is produced and by which adults presumably affect the fitness of the lar-vae.We predict that exploitative competition shapes the behavior of N.littoralis larvae in their aggregations.However,cooperative interactions may also operate in these systems due mainly to the benefits of collective exodigestion.Moreover,indirect parental effects(i.e.,formation of the feeding matrix)probably modulate larval interactions within the ag-gregations.By manipulating parental effects(present/absent)and larval density(0.02-1.9 larvae/g of meat),we found a strong negative group-size effect on fitness components of N.littoralis,in colonies with parental effects over almost the entire density range,and in colonies without parental effects for densities larger than 0.5 larva/g.This was accompa-nied by positive group-size effects in terms of development time(it shortened with lar-val density)and thermogenesis(it increased with larval density).A pronounced positive group-size effect on juvenile fitness was found only in colonies without parental effects and only in the low-density range.These results support the hypothesis that larval societies of N.littoralis are shaped by exploitation competition.

原文链接:

万方数据

Geographic variation in larval cold tolerance and exposure across the invasion front of a widely established forest insect

Petra HafkerLily M.ThompsonJonathan A.WalterDylan Parry...

1930-1942页

查看更多>>摘要：Under global climate change,high and low temperature extremes can drive shifts in species distributions.Across the range of a species,thermal tolerance is based on acclimatization,plasticity,and may undergo selection,shaping resilience to temperature stress.In this study,we measured variation in cold temperature tolerance of early instar lar-vae of an invasive forest insect,Lymantria dispar dispar L.(Lepidoptera:Erebidae),using populations sourced from a range of climates within the current introduced range in the Eastern United States.We tested for population differences in chill coma recovery(CCR)by measuring recovery time following a period of exposure to a nonlethal cold tempera-ture in 2 cold exposure experiments.A 3rd experiment quantified growth responses after CCR to evaluate sublethal effects.Our results indicate that cold tolerance is linked to re-gional climate,with individuals from populations sourced from colder climates recovering faster from chill coma.While this geographic gradient is seen in many species,detecting this pattern is notable for an introduced species founded from a single point-source in-troduction.We demonstrate that the cold temperatures used in our experiments occur in nature during cold spells after spring egg hatch,but impacts to growth and survival ap-pear low.We expect that population differences in cold temperature performance manifest more from differences in temperature-dependent growth than acute exposure.Evaluating intraspecific variation in cold tolerance increases our understanding of the role of climatic gradients on the physiology of an invasive species,and contributes to tools for predicting further expansion.

原文链接:

万方数据
维普

Using kin discrimination to construct synthetic microbial communities of Bacillus subtilis strains impacts the growth of black soldier fly larvae

Jun-Hui ZhaoPing ChengYi WangXun Yan...

1943-1959页

查看更多>>摘要：Using synthetic microbial communities to promote host growth is an effec-tive approach.However,the construction of such communities lacks theoretical guidance.Kin discrimination is an effective means by which strains can recognize themselves from non-self,and construct competitive microbial communities to produce more secondary metabolites.However,the construction of cooperative communities benefits from the widespread use of beneficial microorganisms.We used kin discrimination to construct synthetic communities(SCs)comprising 13 Bacillus subtilis strains from the surface and gut of black soldier fly(BSF)larvae.We assessed larval growth promotion in a pigeon manure system and found that the synthetic community comprising 4 strains(SC 4)had the most profound effect.Genomic analyses of these 4 strains revealed that their comple-mentary functional genes underpinned the robust functionality of the cooperative synthetic community,highlighting the importance of strain diversity.After analyzing the bacterial composition of BSF larvae and the pigeon manure substrate,we observed that SC 4 al-tered the bacterial abundance in both the larval gut and pigeon manure.This also influ-enced microbial metabolic functions and co-occurrence network complexity.Kin discrim-ination facilitates the rapid construction of synthetic communities.The positive effects of SC 4 on larval weight gain resulted from the functional redundancy and complementarity among the strains.Furthermore,SC 4 may enhance larval growth by inducing shifts in the bacterial composition of the larval gut and pigeon manure.This elucidated how the SC promoted larval growth by regulating bacterial composition and provided theoretical guidance for the construction of SCs.

原文链接:

万方数据
维普

Colony performance of three native bumblebee species from South China and association with their gut microbiome

Lei HanZhi-Min ChangChang-Shi RenXiang-Sheng Chen...

1960-1983页

查看更多>>摘要：Bumblebees play an important ecological economic role as pollinators in nature and agriculture.For reasons of biosecurity,many countries promote the cultivation of na-tive bumblebee species for crop pollination instead of importing"alien"species.In South China,a few bumblebee species are considered useful in this way,particularly,Bombus atripes,Bombus bicoloratus and Bombus breviceps.However,whether they are suitable for artificial rearing and forming healthy colonies for pollination,remains unknown.In this project,queens from the 3 native species of Guizhou Province were collected and colonies were started under standardized conditions.The colonies were scored based on 19 parameters,including the stage of colony development,number and weight of off-spring,and diet consumed.The data revealed that B.breviceps had the best performance,produced more workers and consumed the smallest diet.Next,we performed 16S rDNA sequencing of the bacterial communities found in the guts of offspring workers,and then a correlation analysis between colony performance and gut bacteria was conducted.Here,B.breviceps showed the highest diversity in gut bacterial composition,dominated by the bacteria Gilliamella,Snodgrassella,Enterobacter,and Lactobacillus Firm5.The higher the abundance of Snodgrassella,the better the performance of the colony in the founda-tion stage,and later Lactobacillus Firm5,Apibacter and Bifidobacterium were beneficial during the stages of rapid growth and colony decline.Although we do not understand all of the interactions yet,these correlations explain why B.breviceps demonstrated better colony performance.Our data provide valuable information for breeding local Bombus species and will contribute to developing strong colonies for crop pollination.

原文链接:

万方数据