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农业科学学报（英文）
中国农业科学院农业信息研究所
农业科学学报（英文）

中国农业科学院农业信息研究所

翟虎渠

月刊

2095-3119

zgnykx@mail.caas.net.cn

010-82106283 82106280

100081

北京中关村南大街12号

农业科学学报（英文）/Journal Journal of Integrative AgricultureCSCDCSTPCD北大核心SCI
查看更多>>本刊创刊于2002年，由中国农业科学院、中国农学会主办，中国农业科学院农业信息研究所承办。刊登农牧业基础科学和应用科学的研究论文，覆盖作物科学、动物科学、农业环境、农业经济与管理等领域。
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    A novel secreted protein FgHrip1 from Fusarium graminearum triggers immune responses in plants

    Zhenchao FuHuiqian ZhuangVincent NinkuuJianpei Yan...
    3774-3787页
    查看更多>>摘要：Fusarium graminearum,the primary pathogenic fungus responsible for Fusarium head blight(FHB)in wheat,secretes abundant chemical compounds that interact with host plants.In this study,a secreted protein FgHrip1,isolated from the culture filtrate of F.graminearum,was found to induce typical cell death in tobacco.The FgHrip1 gene was then cloned and expressed in Escherichia coli.Further bioassay analysis showed that the recombinant FgHrip1 induced early defense induction events,such as reactive oxygen species(ROS)production,callose deposition,and up-regulation of defense-related genes in tobacco.Furthermore,FgHrip1 significantly enhanced immunity in tobacco seedlings against Pseudomonas syringae pv.tabaci 6605(Pst.6605)and tobacco mosaic virus(TMV).FgHrip1-treated wheat spikes also exhibited defense-related transcript accumulation and developed immunity against FHB infection.Whereas the expression of FgHrip1 was induced during the infection process,the deletion of the gene impaired the virulence of F.graminearum.Our results suggest that FgHrip1 triggers immunity and induces disease resistance in tobacco and wheat,thereby providing new insight into strategy for biocontrol of FHB.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Autophagy-related protein PlAtg3 participates in vegetative growth,sporangial cleavage,autophagy and pathogenicity of Peronophythora litchii

    Chengdong YangManfei LuoXue ZhangLinlin Ye...
    3788-3800页
    查看更多>>摘要：Litchi downy blight,caused by the plant pathogenic oomycete Peronophythora litchii,is one of the most devastating diseases on litchi and resulted in huge economic losses.Autophagy plays an essential role in the development and pathogenicity of the filamentous fungi.However,the function of autophagy in oomycetes remain elusive.Here,an autophagy-related protein Atg3 homolog PlAtg3 was identified and characterized in P.litchii.The absence of PlATG3 through the CRISPR/Cas9 gene replacement strategy compromised vegetative growth and sexual/asexual development.Cytological analyses revealed that the deletion of PlATG3 impaired autophagosome formation with monodansylcadaverine(MDC)staining and significantly disrupted zoospore release due to defects of sporangial cleavage with FM4-64 staining.Atg8 is considered to be an autophagy marker protein in various species.Western blot analysis indicated that PlAtg3 is involved in degradation of PlAtg8-PE.Interestingly,PlAtg3 was unable to interact with PlAtg8 in yeast two hybrid(Y2H)assays,possibly due to the absence of the Atg8 family interacting motif(AIM)in PlAtg3.Furthermore,pathogenicity assays revealed that the deletion of PlATG3 considerably reduced the virulence of P.litchii.Taken together,our data reveal that PlAtg3 plays an important role in radial growth,asexual/sexual development,sporangial cleavage and zoospore release,autophagosome formation,and pathogenicity in P.litchii.This study contributes to a better understanding of the pathogenicity mechanisms of P.litchii and provides insights for the development of more effective strategies to control oomycete diseases.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Disruption of a microvitellogenin gene impairs eggshell formation in Mythimna separata

    Lingling LiJunhong FuChanggeng DaiYuhang Zhou...
    3801-3811页
    查看更多>>摘要：Microvitellogenin(mVg)belongs to the lepidopteran-specific lipoprotein_11 super family and is thought to be involved in insect reproduction.However,the function of mVg in reproduction has not been established.In this study,we identified and characterized MsmVg in Mythimna separata,a destructive insect pest of grain crops.MsmVg is comprised of a 1,310 bp open reading frame(ORF)encoding 437 amino acids.Structural analysis showed that MsmVg contains three conserved domains in the lipoprotein_11 super family,including the all-α-N-terminal domain(NTD),a Pseudomonas putida homologous domain(PPD),and an all-β-C-terminal domain(CTD).MsmVg was highly expressed in mature female adults and enriched in the ovaries of 3-day-old female adults.Disruption of MsmVg by RNA interference did not change the expression of MsmVg,vitellogenin(MsVg)or the vitellogenin receptor(MsVgR)in fat bodies,but it inhibited their transcription in ovaries.Phenotype analysis showed that knockdown of MsmVg did not affect yolk deposition in ovaries,but impaired eggshell formation and led to a reduction in the reproductive capacity.Furthermore,knockdown of MsmVg significantly inhibited the expression of genes associated with eggshell formation(MsChP1-3 and MsFcP3C).Taken together,these data suggest that MsmVg plays an important role in female reproduction by affecting eggshell formation in M.separata,which provides a potential target for managing this insect pest.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Glomerular organization in the antennal lobe of the oriental armyworm Mythimna separata

    Baiwei MaQi ChenXi ChuYidong Zuo...
    3812-3829页
    查看更多>>摘要：In insects,the number and specificity of antennal lobe glomeruli often reflect the number and diversity of expressed chemosensory genes,which are linked to its ecological niche and specific olfactory needs.The oriental armyworm,Mythimna separata,is an important and common lepidopteran pest of cereal crops.Given its reliance on the olfactory system for crucial behaviors,understanding the evolutionary potential of this system requires a thorough characterization of the anatomical structure of the primary olfactory center.Here,we systematically identified all antennal lobe glomeruli of M.separata based on synaptic antibody immunostaining and mass staining of the olfactory sensory neurons.A total of 69 glomeruli were identified in females and 65 in males,and an intuitive nomenclature based on glomerular positions was applied.Our findings uncovered some sex-specific glomeruli in this species.There were ten female-specific glomeruli and three male-specific glomeruli,except for the macroglomerular complex(MGC)units,with a notable observation that the female labial pit organ glomerulus was larger than its male counterpart.Additionally,we identified four antennal-lobe tracts(ALTs)and retrograde labeling from the calyx revealed that all glomeruli were innervated by the medial ALT projection neurons.The comparison of the olfactory system structures between M.separata and sympatric moths supports their evolutionary convergence in noctuid moths.These results collectively lay the foundation for future studies on olfactory processing in M.separata.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Long non-coding RNA FPFSC promotes immature porcine Sertoli cell growth through modulating the miR-326/EHMT2 axis

    Dan ChuBin ChenBo WengSaina Yan...
    3830-3842页
    查看更多>>摘要：Sertoli cells are indispensable for guaranteeing normal spermatogenesis and male fertility.Although a huge number of long non-coding RNAs(lncRNAs)are identified from developing porcine testicular tissues and have been predicted with crucial regulatory roles in spermatogenesis,their functions and regulatory mechanisms are still in infancy.Herein,we mainly explored the regulatory and functional roles of lncFPFSC in proliferation and apoptosis of immature porcine Sertoli cells.The results demonstrated that lncFPFSC was predominantly located in the cytoplasm of immature porcine Sertoli cells.lncFPFSC overexpression promoted cell cycle progression and cell proliferation,as well as inhibited cell apoptosis,whereas siRNA-induced lncFPFSC knockdown resulted in the opposite effects.Mechanistically,lncFPFSC acted as a sponge for miR-326.Overexpression of miR-326 inhibited cell proliferation and induced cell apoptosis,which further abolished the effects of lncFPFSC overexpression.The euchromatic histone-lysine N-methyltransferase 2(EHMT2)gene was directly targeted by miR-326,and its mRNA and protein expressions were both negatively regulated by miR-326 in immature porcine Sertoli cells.Then,siRNA-induced EHMT2 knockdown resulted a similar effect of miR-326 inhibition.Collectively,lncFPFSC promoted proliferation and inhibited apoptosis in immature porcine Sertoli cells through modulating the miR-326/EHMT2 axis.This study expanded our understanding of non-coding RNAs in participating porcine spermatogenesis through deciding the fate of Sertoli cells,and the competing endogenous RNA(ceRNA)network,and provided new molecular markers to treat Sertoli cell disorder inducing male infertility.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Retinol is involved in the intestinal regeneration and strengthens the intestinal barrier during refeeding in broiler chickens

    Youli WangHuajin ZhouJing ChenYuqin Wu...
    3843-3859页
    查看更多>>摘要：Fasting is typically used before feeding metabolizable energy assessment in broilers.Previous studies have shown that fasting cause atrophy of the intestinal villus.Whether fasting affects intestinal permeability during refeeding by altering barrier function and nutrient absorption is of concern.Here,23-d-old broilers were randomly assigned to 5 treatments,fasted for 0,12,24,36,and 48 h,respectively,and then refed for 2 d,to study the impact of different duration of fasting on the intestinal regeneration and barrier function during refeeding.Results showed that the intestinal morphology in fasted birds was recovered in 2 d of refeeding at most.As fasting durations increased,enterocytes per intestinal villus were linearly and quadratically increased(both P<0.05),whereas goblet cells per intestinal villus was linearly decreased(both P<0.05).Besides,the mRNA level of lysozyme was linearly decreased as fasting durations increased during refeeding(both P<0.05),while quadratically increased mucin 2 was observed only after 1 d of refeeding(P<0.05).Linear increase effects were observed for claudin 2 and zonula occludens-1 with increased fasting durations after 1 d of refeeding(all P<0.05),and linear and quadratical effects were observed for claudin 2 at 2 d of refeeding(both P<0.05).Besides,we found that intestinal permeability to creatinine,4 and 70 kD dextran were linearly and quadratically decreased with increased fasting durations at 6 h and 1 d of refeeding(all P<0.05).Furthermore,jejunum proteomic from birds refed for 6 h showed that birds fasted for 36 h showed increased antimicrobial peptides and upregulated retinol metabolism when compared to the nonfasted birds(P<0.05).Further study showed that retinyl ester catabolism was inhibited during fasting and enhanced during refeeding.Results of intestinal organoid culture showed that retinol benefits the cell proliferation and enterocyte differentiation.In conclusion,the intestinal permeability to small and large molecules was decreased during refeeding by strengthening the intestinal barrier function,and the activated retinol metabolism during refeeding is involved in the intestinal regeneration and strengthens the intestinal barrier.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Genetic and biological properties of H10Nx influenza viruses in China

    Yina XuHailing LiHaoyu LengChaofan Su...
    3860-3869页
    查看更多>>摘要：H10 subtype avian influenza viruses(AIV)have been circulating in China for 40 years.H10 AIVs in China have expanded their host range from wild birds to domestic poultry and mammals,even human.Most of the H10 subtype AIVs reported in China were isolate from the southeast part.We isolated an H10N3 AIV,A/Chicken/Liaoning/SY1080/2021(SY1080),from live poultry market(LPM)in Liaoning Province of the Northeast China.SY1080 replicated efficiently in mice lungs and nasal turbinates without prior adaptation.We systematically compared SY1080 with other H10 subtype isolates in China.Phylogenetic analysis showed that SY1080 and most of the H10 strains belonged to the Eurasian lineage.H10 AIVs in China have formed 63 genotypes.SY1080 as well as the H10N3 strains from human infections belonged to G60 genotype.H10Nx AIV acquired multiple mammalian adaptive and virulence related mutations during circulation and the recent reassortants derived internal genes from chicken H9N2 AIVs.The H10Nx subtypes AIVs posed potential threat to public health.These results suggested we should strengthen the surveillance and evaluation of H10 subtype strains.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Straw interlayer improves sunflower root growth:Evidence from moisture and salt migration and the microbial community in saline-alkali soil

    Mengmeng ChenGuoli WangYupeng JingJie Zhou...
    3870-3881页
    查看更多>>摘要：A straw interlayer added to soil can effectively reduce soil salinity effects on plant growth,however,the effects of soil moisture,salt and microbial community composition on plant growth under a straw interlayer are unclear.A rhizobox study was conducted to investigate the role of straw interlayer thickness on soil moisture,salt migration,microbial community composition,as well as root growth in sunflower.The study included four treatments:Control(no straw interlayer);S3(straw interlayer of 3.0 cm);S5(straw interlayer of 5.0 cm);S7(straw interlayer of 7.0 cm).Straw interlayer treatments increased soil moisture by 8.2-11.0%after irrigation and decreased soil salt content after the bud stage in 0-40 cm soil.Total root length,total root surface area,average root diameter,total root volume and the number of root tips of sunflower plants were higher under straw interlayer treatments than in the control,and were the highest under the S5 treatment.This stimulated root growth was ascribed to the higher abundance of Chloroflexi and Verrucomicrobia bacteria in soil with a straw interlayer,which was increased by 55.7 and 54.7%,respectively,in the S5 treatment.Addition of a straw interlayer of 5 cm thickness is a practical and environmentally feasible approach for improving sunflower root growth in saline-alkali soil.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Biochar induced trade-offs and synergies between ecosystem services and crop productivity

    Jinxia WangQiu HuangKai PengDayang Yang...
    3882-3895页
    查看更多>>摘要：Biochar amendment offers a chance for sustainable agriculture.However,the effectiveness of biochar relies on its physical and chemical properties,which are heavily affected by biochar production conditions and management practices.Therefore,substantial uncertainties regarding the use of biochar exist in agricultural systems globally.This study provides the first quantitative evaluation of the impacts of biochar characteristics and management practices on key ecosystem services by performing a second-order meta-analysis based on 34,628 paired observations in biochar-amended and unamended systems.Overall,biochar enhances phytotoxicity alleviation,physiology regulation,soil remediation and carbon sequestration,and microbial functional gene abundance.However,some prominent trade-offs exist between crop productivity and ecosystem service deliveries including for nutrient cycling,microbial function,climate change mitigation,and the soil microbial community.The adoption of low C:N biochar produced at high pyrolysis temperatures from sewage sludge-derived feedstock,in combination with a moderate application rate and inorganic fertilizer input,shows potential for achieving synergistic promotion of crop productivity and ecosystem services.These outcomes highlight the need for judicious implementation of biochar-based solutions to site-specific soil constraints.The quantified synergy and tradeoffrelationships will aid the establishment of a sustainable biochar development framework that strengthens necessary ecosystem services commensurate with food security assurance.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Insight into the effect of geographic location and intercropping on contamination characteristics and exposure risk of phthalate esters(PAEs)in tea plantation soils

    Jie LiShanjie HanRuhang XuXuchen Zhang...
    3896-3911页
    查看更多>>摘要：Phthalate esters(PAEs)are an emerging pollutant due to widespread distribution in environmental mediums that have attracted widespread attention over recent years.However,there is little information about tea plantation soil PAEs.A total of 270 soil samples collected from 45 tea plantations in the major high-quality tea-producing regions of Jiangsu,Zhejiang,and Anhui provinces in China were analyzed for seven PAEs.The detection frequency of PAEs in tea plantation soil was 100%.DBP,DEHP,and DiBP were the main congeners in tea plantation soil.The PAEs concentrations in the upper soil were significantly higher than those in the lower soil.The concentration of tea plantation soil PAEs in Jiangsu Province was significantly lower than those in Zhejiang and Anhui provinces.Intercropping with chestnuts can effectively reduce the contamination level of PAEs in tea plantation soil.Correlation analysis,redundancy analysis,partial correlation analysis,and structural equation modeling methods further confirmed the strong direct influence of factors such as chestnut-tea intercropping,temperature,and agricultural chemicals on the variation of PAEs in tea plantation soil.The health and ecological risk assessments indicated that non-carcinogenic risk was within a safe range and that there was a high carcinogenic risk via the dietary pathway,with DBP posing the highest ecological risk.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据