查看更多>>摘要：Since the outbreak of the novel corona virus disease 2019 (COVID-19) at the end of 2019,specific antiviral drugs have been lacking.A Chinese patent medicine Toujiequwen granules has been promoted in the treatment of COVID-19.The present study was designed to reveal the molecular mechanism of Toujiequwen granules against COVID-19.A network pharmacological method was applied to screen the main active ingredients of Toujiequwen granules.Network analysis of 149 active ingredients and 330 drug targets showed the most active ingredient interacting with many drug targets is quercetin.Drug targets most affected by the active ingredients were PTGS2,PTGS 1,and DPP4.Drug target disease enrichment analysis showed drug targets were significantly enriched in cardiovascular diseases and digestive tract diseases.An "active ingredienttarget-disease" network showed that 57 active ingredients from Toujiequwen granules interacted with 15 key targets of COVID-19.There were 53 ingredients that could act on DPP4,suggesting that DPP4 may become a potential new key target for the treatment of COVID-19.GO analysis results showed that key targets were mainly enriched in the cellular response to lipopolysaccharide,cytokine activity and other functions.KEGG analysis showed they were mainly concentrated in viral protein interaction with cytokine and cytokine receptors and endocrine resistance pathway.The evidence suggests that Toujiequwen granules might play an effective role by improving the symptoms of underlying diseases in patients with COVID-19 and multi-target interventions against multiple signaling pathways related to the pathogenesis of COVID-19.
查看更多>>摘要：This retrospective study aimed to investigate the clinical characteristics and neonatal outcomes of pregnant women with SARS-COV-2 in Wuhan Children's Hospital and further suggested a possible management strategy for infected pregnant women under epidemic situation.In this study,8 pregnant women with SARS-COV-2 who were admitted into Wuhan Children's Hospital,China from February 1,2020 to March 30,2020 and the clinical features,laboratory data,maternal and neonatal outcomes were analyzed.The mean age of the women at the time of admission was 30.6 years.The mean gestational age of the women was 37 weeks+4 days,and one woman presented with dichorionic diamniotic (DCDA) twin pregnancy.Except for one woman who was febrile,others had no typical clinical symptoms.For all pregnant women,the count of white blood cells and lymphocytes appeared normal,but 6 had a lower percentage of lymphocytes.C-reactive protein (CRP) levels were normal for all the women.One neonate was tested positive for the coronavirus IgG and IgM antibodies.The clinical symptoms of the pregnant women with SARS-COV-2 were mild,and the laboratory data showed similar characteristics to those of non-infected pregnant women.Since one neonate was tested positive for coronavirus,there is a possibility of vertical transmission of SARS-CoV-2.Prompt and efficient screening,triage,and isolation of pregnant women are effective management strategies to reduce nosocomial infection during the SARS-COV-2 epidemic.
查看更多>>摘要：This retrospective study aimed to investigate the clinical characteristics and neonatal outcomes of pregnant women with SARS-COV-2 in Wuhan Children's Hospital and further suggested a possible management strategy for infected pregnant women under epidemic situation.In this study,8 pregnant women with SARS-COV-2 who were admitted into Wuhan Children's Hospital,China from February 1,2020 to March 30,2020 and the clinical features,laboratory data,maternal and neonatal outcomes were analyzed.The mean age of the women at the time of admission was 30.6 years.The mean gestational age of the women was 37 weeks+4 days,and one woman presented with dichorionic diamniotic (DCDA) twin pregnancy.Except for one woman who was febrile,others had no typical clinical symptoms.For all pregnant women,the count of white blood cells and lymphocytes appeared normal,but 6 had a lower percentage of lymphocytes.C-reactive protein (CRP) levels were normal for all the women.One neonate was tested positive for the coronavirus IgG and IgM antibodies.The clinical symptoms of the pregnant women with SARS-COV-2 were mild,and the laboratory data showed similar characteristics to those of non-infected pregnant women.Since one neonate was tested positive for coronavirus,there is a possibility of vertical transmission of SARS-CoV-2.Prompt and efficient screening,triage,and isolation of pregnant women are effective management strategies to reduce nosocomial infection during the SARS-COV-2 epidemic.
查看更多>>摘要：We here aimed to investigate the impact of gender on the clinical characteristics and laboratory results of patients with coronavirus disease 2019 (COVID-19) and provide clues to the pathological mechanisms underlying COVID-19.A retrospective study was performed.Clinical characteristics,severity of lung infection,laboratory results,and prognoses of patients of different gender were analyzed.A total of 242 patients were finally included.The median age was 58 years (IQR: 40-68),including 54 (22.3％) hospital staffs.Ninety-four (38.8％) were male and 148 (61.1％) were female.The proportion of patients with diabetes was significantly higher in the male group than in the female group (P=0.034).Male patients had a significantly larger proportion of severe lung infection,higher leukocyte count,neutrophil count,neutrophil-to-lymphocyte ratio,C-reactive protein,and procalcitonin than female.Furthermore,male patients had worse liver,cardiac,and coagulation function than their female counterparts.Male patients with COVID-19 showed more severe inflammation reaction and coagulation dysfunction than female patients.In conclusion,gender is associated with host response to SARS-CoV-2 infection.
查看更多>>摘要：We here aimed to investigate the impact of gender on the clinical characteristics and laboratory results of patients with coronavirus disease 2019 (COVID-19) and provide clues to the pathological mechanisms underlying COVID-19.A retrospective study was performed.Clinical characteristics,severity of lung infection,laboratory results,and prognoses of patients of different gender were analyzed.A total of 242 patients were finally included.The median age was 58 years (IQR: 40-68),including 54 (22.3％) hospital staffs.Ninety-four (38.8％) were male and 148 (61.1％) were female.The proportion of patients with diabetes was significantly higher in the male group than in the female group (P=0.034).Male patients had a significantly larger proportion of severe lung infection,higher leukocyte count,neutrophil count,neutrophil-to-lymphocyte ratio,C-reactive protein,and procalcitonin than female.Furthermore,male patients had worse liver,cardiac,and coagulation function than their female counterparts.Male patients with COVID-19 showed more severe inflammation reaction and coagulation dysfunction than female patients.In conclusion,gender is associated with host response to SARS-CoV-2 infection.
查看更多>>摘要：Serology tests for viral antibodies provide an important tool to support nucleic acid testing for diagnosis of the novel coronavirus disease 2019 (COVID-19) and is useful for documenting previous exposures to SARS-CoV-2,the etiological agent of COVID-19.The sensitivities of the chemiluminescent SARS-CoV-2 IgG/IgM immunoassay were assessed by using serum samples collected from 728 patients testing positive for SARS-CoV-2 RNA.The specificity was evaluated on a panel of 60 serum samples from non-COVID-19 patients with high levels of rheumatoid factor,antinuclear antibody,or antibodies against Epstein-Barr virus (EBV),cytomegalovirus (CMV),mycoplasma pneumonia,human respiratory syncytial virus (RSV),adenovirus,influenza A or influenza B.The imprecision and interference were assessed by adopting the Clinical and Laboratory Standards Institute (CLSI) EP15-A2 and EP7-A2,respectively.Sensitivities between 1 and 65 days after onset of symptoms were 94.4％ and 78.7％,for IgG and IgM test,respectively.The sensitivity increased with the time after symptom onset,and rose to the top on the 22nd to 28th days.The total imprecision (CVs) was less than 6.0％ for IgG and less than 6.5％ for IgM.Limited cross-reactions with antibodies against EBV,CMV,mycoplasma pneumonia,human RSV,adenovirus,influenza A or influenza B were found.These data suggested the chemiluminescent SARS-CoV-2 IgG and IgM,assay with reliable utility and sensitivity,could be used for rapid screening and retrospective surveillance of COVID-19.
查看更多>>摘要：Serology tests for viral antibodies provide an important tool to support nucleic acid testing for diagnosis of the novel coronavirus disease 2019 (COVID-19) and is useful for documenting previous exposures to SARS-CoV-2,the etiological agent of COVID-19.The sensitivities of the chemiluminescent SARS-CoV-2 IgG/IgM immunoassay were assessed by using serum samples collected from 728 patients testing positive for SARS-CoV-2 RNA.The specificity was evaluated on a panel of 60 serum samples from non-COVID-19 patients with high levels of rheumatoid factor,antinuclear antibody,or antibodies against Epstein-Barr virus (EBV),cytomegalovirus (CMV),mycoplasma pneumonia,human respiratory syncytial virus (RSV),adenovirus,influenza A or influenza B.The imprecision and interference were assessed by adopting the Clinical and Laboratory Standards Institute (CLSI) EP15-A2 and EP7-A2,respectively.Sensitivities between 1 and 65 days after onset of symptoms were 94.4％ and 78.7％,for IgG and IgM test,respectively.The sensitivity increased with the time after symptom onset,and rose to the top on the 22nd to 28th days.The total imprecision (CVs) was less than 6.0％ for IgG and less than 6.5％ for IgM.Limited cross-reactions with antibodies against EBV,CMV,mycoplasma pneumonia,human RSV,adenovirus,influenza A or influenza B were found.These data suggested the chemiluminescent SARS-CoV-2 IgG and IgM,assay with reliable utility and sensitivity,could be used for rapid screening and retrospective surveillance of COVID-19.
查看更多>>摘要：Neutrophilic airway inflammation is one of the features of severe asthma.Neutrophil gelatinase-associated lipocalin (NGAL),or lipocalin-2,is a glycoprotein associated with neutrophilic inflammation and can be detected in blood.Recently,blood NGAL levels have been reported to be elevated in chronic obstructive pulmonary disease.However,the clinical significance of serum NGAL levels in patients with asthma has not been elucidated.The aim of this study was to explore the association between serum NGAL level and clinical parameters in patients with asthma.Sixty-one non-smoking people with stable asthma were enrolled in this study.All patients underwent blood collection and pulmonary function tests.The associations between serum NGAL levels and clinical parameters were analyzed retrospectively.Serum NGAL levels in patients with asthma and obstructive ventilatory defect were higher than those in patients with asthma without obstructive ventilatory defect (76.4±51.4 ng/mL vs.39.3±27.4 ng/mL,P=0.0019).Serum NGAL levels were correlated with forced expired flow at 50％ of vital capacity ％predicted and forced expired flow at 75％ of vital capacity ％predicted (r=-0.3373,P=0.0078 and r=-0.2900,P=0.0234,respectively).Results of a multiple regression analysis demonstrated that serum NGAL level was independently associated with obstructive ventilatory defect.Serum NGAL levels were elevated in patients with asthma and obstructive ventilatory defect.NGAL may be involved in airway remodeling possibly mediated by neutrophilic inflammation in asthma.
查看更多>>摘要：Neutrophilic airway inflammation is one of the features of severe asthma.Neutrophil gelatinase-associated lipocalin (NGAL),or lipocalin-2,is a glycoprotein associated with neutrophilic inflammation and can be detected in blood.Recently,blood NGAL levels have been reported to be elevated in chronic obstructive pulmonary disease.However,the clinical significance of serum NGAL levels in patients with asthma has not been elucidated.The aim of this study was to explore the association between serum NGAL level and clinical parameters in patients with asthma.Sixty-one non-smoking people with stable asthma were enrolled in this study.All patients underwent blood collection and pulmonary function tests.The associations between serum NGAL levels and clinical parameters were analyzed retrospectively.Serum NGAL levels in patients with asthma and obstructive ventilatory defect were higher than those in patients with asthma without obstructive ventilatory defect (76.4±51.4 ng/mL vs.39.3±27.4 ng/mL,P=0.0019).Serum NGAL levels were correlated with forced expired flow at 50％ of vital capacity ％predicted and forced expired flow at 75％ of vital capacity ％predicted (r=-0.3373,P=0.0078 and r=-0.2900,P=0.0234,respectively).Results of a multiple regression analysis demonstrated that serum NGAL level was independently associated with obstructive ventilatory defect.Serum NGAL levels were elevated in patients with asthma and obstructive ventilatory defect.NGAL may be involved in airway remodeling possibly mediated by neutrophilic inflammation in asthma.
Jin Hyoung KimJong Joon AhnYangjin JegaSoohyun Bae...
329-335页
查看更多>>摘要：To apply a new airway treatment to humans,preclinical studies in an appropriate animal model is needed.Canine,porcine and leporine tracheas have been employed as animal airway stenosis models using various methods such as chemical caustic agents,laser,and electrocautery.However,existing models take a long time to develop (3-8 weeks) and the mechanism of stenosis is different from that in humans.The aim of the present study was to establish a new and fast tracheal stenosis model in pigs using a combination of cuff overpressure intubation (COI) and electrocautery.Fourteen pigs were divided into three groups: tracheal cautery (TC) group (n=3),COI group (n=3),and COI-TC combination group (n=8).Cuff overpressure (200/400/500 mmHg) was applied using a 9-mm endotracheal tube.Tracheal cautery (40/60 watts) was performed using a rigid bronchoscopic electrocoagulator.After intervention,the pigs were observed for 3 weeks and bronchoscopy was performed every 7 days.When the cross-sectional area decreased by ＞ 50％,it was confirmed that tracheal stenosis was established.The time for tracheal stenosis was 14 days in the TC group and 7 days in the COI-TC combination group.In the COI group,no stenosis occurred.In the COI-TC group,electrocautery (40 watts) immediately after intubation for ＞ 1 h with a cuff pressure of 200 mmHg or more resulted in sufficient tracheal stenosis within 7 days.Moreover,the degree of tracheal stenosis increased in proportion to the cuff pressure and tracheal intubation time.The combined use of cuff overpressure and electrocautery helped to establish tracheal stenosis in pigs rapidly.
万方数据
NETL
NSTL
维普