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分子植物（英文版）
分子植物（英文版）编辑部
分子植物（英文版）

分子植物（英文版）编辑部

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1674-2052

molplant@gmail.com

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上海市岳阳路319号31B楼

分子植物（英文版）/Journal Molecular PlantCSCDCSTPCD北大核心SCI
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    Plant grafting:Molecular mechanisms and applications

    Ming FengFrauke AugsteinAbdul KareemCharles W.Melnyk...
    75-91页
    查看更多>>摘要：People have grafted plants since antiquity for propagation,to increase yields,and to improve stress toler-ance.This cutting and joining of tissues activates an incredible regenerative ability as different plants fuse and grow as one.For over a hundred years,people have studied the scientific basis for how plants graft.Today,new techniques and a deepening knowledge of the molecular basis for graft formation have allowed a range of previously ungraftable combinations to emerge.Here,we review recent developments in our un-derstanding of graft formation,including the attachment and vascular formation steps.We analyze why plants graft and how biotic and abiotic factors influence successful grafting.We also discuss the ability and inability of plants to graft,and how grafting has transformed both horticulture and fundamental plant science.As our knowledge about plant grafting improves,new combinations and techniques will emerge to allow an expanded use of grafting for horticultural applications and to address fundamental research questions.
      原文链接:
    • 万方数据
    • 维普

    Immunobiodiversity:Conserved and specific immunity across land plants and beyond

    Baptiste CastelKarima El MahboubiChristophe JacquetPierre-Marc Delaux...
    92-111页
    查看更多>>摘要：Angiosperms represent most plants that humans cultivate,grow,and eat.However,angiosperms are only one of five major land plant lineages.As a whole lineage,plants also include algal groups.All these clades repre-sent a tremendous genetic diversity that can be investigated to reveal the evolutionary history of any given mechanism.In this review,we describe the current model of the plant immune system,discuss its evolution based on the recent literature,and propose future directions for the field.In angiosperms,plant-microbe in-teractions have been intensively studied,revealing essential cell surface and intracellular immune receptors,as well as metabolic and hormonal defense pathways.Exploring diversity at the genomic and functional levels demonstrates the conservation of these pathways across land plants,some of which are beyond plants.On basis of the conserved mechanisms,lineage-specific variations have occurred,leading to diversified reser-voirs of immune mechanisms.In rare cases,this diversity has been harnessed and successfully transferred to other species by integration of wild immune receptors or engineering of novel forms of receptors for improved resistance to pathogens.We propose that exploring further the diversity of immune mechanisms in the whole plant lineage will reveal completely novel sources of resistance to be deployed in crops.
      原文链接:
    • 万方数据
    • 维普

    Woody plant cell walls:Fundamentals and utilization

    Wei LiYing-Chung Jimmy LinYing-Lan ChenChenguang Zhou...
    112-140页
    查看更多>>摘要：Cell walls in plants,particularly forest trees,are the major carbon sink of the terrestrial ecosystem.Chem-ical and biosynthetic features of plant cell walls were revealed early on,focusing mostly on herbaceous model species.Recent developments in genomics,transcriptomics,epigenomics,transgenesis,and asso-ciated analytical techniques are enabling novel insights into formation of woody cell walls.Here,we review multilevel regulation of cell wall biosynthesis in forest tree species.We highlight current approaches to en-gineering cell walls as potential feedstock for materials and energy and survey reported field tests of such engineered transgenic trees.We outline opportunities and challenges in future research to better under-stand cell type biogenesis for more efficient wood cell wall modification and utilization for biomaterials or for enhanced carbon capture and storage.
      原文链接:
    • 万方数据
    • 维普

    Perenniality:From model plants to applications in agriculture

    Bo ZhaoJia-Wei Wang
    141-157页
    查看更多>>摘要：To compensate for their sessile nature,plants have evolved sophisticated mechanisms enabling them to adapt to ever-changing environments.One such prominent feature is the evolution of diverse life history strategies,particularly such that annuals reproduce once followed by seasonal death,while perennials live longer by cycling growth seasonally.This intrinsic phenology is primarily genetic and can be altered by environmental factors.Although evolutionary transitions between annual and perennial life history stra-tegies are common,perennials account for most species in nature because they survive well under year-round stresses.This proportion,however,is reversed in agriculture.Hence,perennial cropspromise to likewise protect and enhance the resilience of agricultural ecosystems in response to climate change.Despite significant endeavors that have been made to generate perennial crops,progress is slow because of barriers in studying perennials,and many developed species await further improvement.Recent findings in model species have illustrated that simply rewiring existing genetic networks can lead to lifestyle varia-tion.This implies that engineering plant life history strategy can be achieved by manipulating only a few key genes.In this review,we summarize our current understanding of genetic basis of perenniality and discuss major questions and challenges that remain to be addressed.
      原文链接:
    • 万方数据
    • 维普

    Multiomics analyses of two Leonurus species illuminate leonurine biosynthesis and its evolution

    Peng LiMeng-Xiao YanPan LiuDan-Jie Yang...
    158-177页
    查看更多>>摘要：The Lamiaceae family is renowned for its terpenoid-based medicinal components,but Leonurus,which has traditional medicinal uses,stands out for its alkaloid-rich composition.Leonurine,the principal active com-pound found in Leonurus,has demonstrated promising effects in reducing blood lipids and treating strokes.However,the biosynthetic pathway of leonurine remains largely unexplored.Here,we present the chromosome-level genome sequence assemblies of Leonurus japonicus,known for its high leonurine production,and Leonurus sibiricus,characterized by very limited leonurine production.By integrating ge-nomics,RNA sequencing,metabolomics,and enzyme activity assay data,we constructed the leonurine biosynthesis pathway and identified the arginine decarboxylase(ADC),uridine diphosphate glucosyltrans-ferase(UGT),and serine carboxypeptidase-like(SCPL)acyltransferase enzymes that catalyze key reac-tions in this pathway.Further analyses revealed that the UGT-SCPL gene cluster evolved by gene duplica-tion in the ancestor of Leonurus and neofunctionalization of SCPL in L.japonicus,which contributed to the accumulation of leonurine specifically in L.japonicus.Collectively,our comprehensive study illuminates leonurine biosynthesis and its evolution in Leonurus.
      原文链接:
    • 万方数据
    • 维普

    A new oxidative pathway of nitric oxide production from oximes in plants

    Pedro López-GómezJavier BuezoMarina UrraAlfonso Cornejo...
    178-198页
    查看更多>>摘要：Nitric oxide(NO)is an essential reactive oxygen species and a signal molecule in plants.Although several studies have proposed the occurrence of oxidative NO production,only reductive routes for NO production,such as the nitrate(NO-3)-upper-reductase pathway,have been evidenced to date in land plants.However,plants grown axenically with ammonium as the sole source of nitrogen exhibit contents of nitrite and NO3-,evidencing the existence of a metabolic pathway for oxidative production of NO.We hypothesized that ox-imes,such as indole-3-acetaldoxime(IAOx),a precursor to indole-3-acetic acid,are intermediate oxidation products in NO synthesis.We detected the production of NO from IAOx and other oximes catalyzed by peroxidase(POD)enzyme using both 4-amino-5-methylamino-2',7'-difluorescein fluorescence and chem-iluminescence.Flavins stimulated the reaction,while superoxide dismutase inhibited it.Interestingly,mouse NO synthase can also use IAOx to produce NO at a lower rate than POD.We provided a full mech-anism for POD-dependent NO production from IAOx consistent with the experimental data and supported by density functional theory calculations.We showed that the addition of IAOx to extracts from Medicago truncatula increased the in vitro production of NO,while in vivo supplementation of IAOx and other oximes increased the number of lateral roots,as shown for NO donors,and a more than 10-fold increase in IAOx dehydratase expression.Furthermore,we found that in vivo supplementation of IAOx increased NO pro-duction in Arabidopsis thaliana wild-type plants,while prx33-34 mutant plants,defective in POD33-34,had reduced production.Our data show that the release of NO by IAOx,as well as its auxinic effect,explain the superroot phenotype.Collectively,our study reveals that plants produce NO utilizing diverse molecules such as oximes,POD,and flavins,which are widely distributed in the plant kingdom,thus intro-ducing a long-awaited oxidative pathway to NO production in plants.This knowledge has essential impli-cations for understanding signaling in biological systems.
      原文链接:
    • 万方数据
    • 维普

    GreenPhos,a universal method for in-depth measurement of plant phosphoproteomes with high quantitative reproducibility

    Xiaoxiao DuanYuanya ZhangXiahe HuangXiao Ma...
    199-213页
    查看更多>>摘要：Protein phosphorylation regulates a variety of important cellular and physiological processes in plants.In-depth profiling of plant phosphoproteomes has been more technically challenging than that of animal phos-phoproteomes.This is largely due to the need to improve proteinextraction efficiency from plant cells,which have a dense cell wall,and to minimize sample loss resulting from the stringent sample clean-up steps required for the removal of a large amount of biomolecules interfering with phosphopeptide purifica-tion and mass spectrometry analysis.To this end,we developed a method with a streamlined workflow for highly efficient purification of phosphopeptides from tissues of various green organisms includingArabi-dopsis,rice,tomato,and Chlamydomonas reinhardtii,enabling in-depth identification with high quantita-tive reproducibility of about 11 000 phosphosites,the greatest depth achieved so far with single liquid chromatography-mass spectrometry(LC-MS)runs operated in a data-dependent acquisition(DDA)mode.The mainstay features of the method are the minimal sample loss achieved through elimination of sample clean-up before protease digestion and of desalting before phosphopeptide enrichment and hence the dramatic increases of time-and cost-effectiveness.The method,named GreenPhos,combined with single-shot LC-MS,enabled in-depth quantitative identification of Arabidopsis phosphoproteins,including differentially phosphorylated spliceosomal proteins,at multiple time points during salt stress and a number of kinase substrate motifs.GreenPhos is expected to serve as a universal method for purification of plant phosphopeptides,which,if samples are further fractionated and analyzed by multiple LC-MS runs,could enable measurement of plant phosphoproteomes with an unprecedented depth using a given mass spec-trometry technology.
      原文链接:
    • 万方数据
    • 维普