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海南医科大学学报（英文版）
海南医科大学学报（英文版）

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海南医科大学学报（英文版）/Journal Journal of Hainan Medical University
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    Analysis of laboratory and imaging examination results of patients with COVID-192 years after discharge in Chengdu

    Zhao Ben-NanDu QingKang JunLiu Da-feng...
    1-7页
    查看更多>>摘要：Objective: To summarize the follow‑up results of laboratory examination, echocardiographic and chest CT of patients with COVID‑19 at the time of 2 years after discharge in Chengdu. Methods: A total of 29 COVID‑19 survivors who have participated in the 1‑year follow‑up and 2‑year follow‑up at Public Health Clinical Center of Chengdu were included in our study. Their blood laboratory tests, echocardiography and chest CT results were analyzed in order to evaluate the long‑term recovery of COVID‑19 survivors. Results: The most common abnormal laboratory test results at the 2‑year follow‑up were increased serum fibronectin (21 cases, 72.4％) and decreased NK cell counts (19 cases, 65.5％). Compared with laboratory test results at the 1‑year follow‑up, platelet count, hydroxybutyrate dehydrogenase and creatine kinase isoenzyme were decreased (all P<0.05), while serum fibrinogen, triglyceride, insulin and non‑specific immunoglobulin A were increased (all P<0.05). Serum specific total SARS‑CoV‑2 antibody amount in COVID‑19 survivors at half a year after COVID‑19 vaccine was significantly higher than that before vaccination. More than half of patients (55.2％) had normal echocardiography results at the 2‑year follow‑up, the main abnormal results of them were valve regurgitation (7 cases, 24.1％) and reduced left ventricular diastolic function (9 cases, 31.0％). Compared with 1‑year follow‑up, 7 patients had fewer abnormal cardiac ultrasound results. 28 cases underwent chest CT at the 2‑year follow‑up, the mainly abnormal results were ground glass shadow (17.9％), pulmonary nodules (diameter<6mm) (32.1％) and scattered cable shadow (39.3％). 71.4％ (20/28) of them had no significant change between the two chest CT results. Conclusion: The mainly abnormal blood laboratory indicators in COVID‑19 survivors at 2‑year follow‑up after discharge were increased serum fibronectin and decreased NK cell counts. Although echocardiography and chest CT results were no significant change between 1‑year follow‑up and 2‑year follow‑up among COVID‑19 survivors, there were still some patients whose abnormal results were decreasing. Serum specific total SARS‑CoV‑2 antibody amount in COVID‑19 survivors at half a year after COVID‑19 vaccine was still significantly higher than that before vaccination, but the amount of specific antibody had a downward trend with time.
      原文链接:
    • 万方数据
    • 维普

    Exploration of the effective components and mechanism of Alpinia oxyphylla-Saposhnikovia Radix in the treatment of diabetic kidney disease based on network pharmacology

    Rui KaiWang XianZhao Jing-YuMa Tian-Peng...
    8-13页
    查看更多>>摘要：Objective: Network pharmacology was used to predict the main targets and related mechanism pathways of the key compounds and active ingredients of the Alpinia oxyphylla-Saposhnikovia Radix based on the therapeutic strategy of tonifying kidney and dispelling pathogenic wind for the treatment of diabetic kidney disease(DKD). Methods: The effective components of Alpinia oxyphylla-Saposhnikovia Radix were screened through the data platform of Traditional Chinese Medicine(TCM). After screening effective targets, the disease target gene database was used to obtain targets for DKD, and then gene enrichment analysis to predict relevant signalling pathways after obtaining the key target genes through Venn analysis. Results: There were 324 active components of Alpinia oxyphylla-Saposhnikovia Radix through the data platform of TCM. In addition, Venn analysis showed that there were 48 key target genes for the treatment of DKD. The biological pathways for the action of Alpinia oxyphylla-Saposhnikovia Radix in DKD were closely related to many signaling pathways including NF-kappaB, classical MAP kinase, JNK and P38 MAP kinase, and ERK5. Conclusions: Treatment of DKD with Alpinia oxyphylla-Saposhnikovia Radix based on the therapeutic strategy of tonifying kidney and dispelling pathogenic wind has the characteristics of multi-components, multi-targets and multi-pathways, which could provide new ideas and methods for further researches on the treatment of DKD with Alpinia oxyphylla-Saposhnikovia Radix.
      原文链接:
    • 万方数据
    • 维普

    Effect of Acacetin on flagellin induced NLRC4 inflammasome activation in mouse bone marrow-derived macrophages

    Wang Zhao-xiaBu JuanZhang Xiao-lingMAHAN Yeledan...
    14-17页
    查看更多>>摘要：Objective: To investigate the effect of acacetin on flagellin induced NLRC4 inflammasome activation in mouse bone marrow-derived macrophages (BMDMs). Methods: Mouse BMDMs were divided into control group, LPS group, LPS + flagellin group and LPS + acacetin + flagellin group. All groups were added with complete medium, then primed with LPS (50 ng/mL) for 3 hrs except the control group, whereafter, LPS + flagellin group was treated with flagellin (10 μmol/L) for 0.5 hr and LPS + acacetin + flagellin group was treated with acacetin (10 μmol/L) for 0.5 hr following by flagellin (10 μmol/L) for 0.5 hr. Pro-caspase-1, pro-IL-1β in cell lysate and caspase-1, IL-1β in supernatant were detected by Western blot (WB). IL-1β, IL-18 and TNF-α in supernatant were measured by enzyme-linked immunosorbent assay (ELISA). And the activity of LDH in supernatant was assessed by LDH test kit. Results: Compared with the control group, in LPS + flagellin group, the expression of caspase-1, IL-1β protein in supernatant were significantly increased (all P-values<0.05), but the differences of the expression of pro-caspase-1 and pro-IL-1β protein in cell lysate were not significant. Compared with LPS + flagellin group, in LPS + acacetin + flagellin group, the expression of caspase-1, IL-1β protein in supernatant were significantly reduced (all P-values<0.05), while the differences of the expression of pro-caspase-1 and pro-IL-1β protein in cell lysate were not significant. ELISA showed that compared with the control group, the levels of IL-1β, IL-18, and TNF-α and the activity of LDH in supernatant of LPS + flagellin group were significantly increased (all P-values<0.05). Compared with LPS + flagellin group, in LPS + Acacetin + flagellin group, the level of IL-1β in supernatant was significantly decreased (P<0.05), meanwhile, the decreases of the levels IL-18, TNF-α and the activity of LDH were not significant. Conclusions: We found that Acacetin can effectively inhibit flagellin induced NLRC4 inflammasome activation and reduce cell damage in mouse BMDMs.
      原文链接:
    • 万方数据
    • 维普

    Kaempferol attenuates knee osteoarthritis via inhibiting cartilage apoptosis in mice

    Mi Yi-linYi Nan-xingXu Xiao-tongYang Luo-jian...
    18-25页
    查看更多>>摘要：Objective: To study the mechanism of kaempferol in the intervention of knee osteoarthritis (KOA) in mice by inhibiting cartilage apoptosis. Methods: Firstly, the target genes of kaempferol were retrieved via TCMSP, and the genes related to KOA were obtained by GeneCards, OMIM, PharmGKB, TTD, and Drugbank databases. Then GO enrichment analysis and KEGG signaling pathway analysis were also performed. Subsequently,18 male C57 mice were randomly divided into the sham operation group, the model group, and the kaempferol group (50 mg/kg). Except for the sham operation group, the KOA mouse model was induced by destabilization of medial meniscus surgery. The sham operation group and model group were given the same amount of normal saline daily for 8 weeks while the kaempferol group was given 50 mg/kg kaempferol intragastrically. Results: A total of 63 targets of kaempferol were found that included 35 common target genes with KOA. GO and KEGG analyses showed that biological processes such as extrinsic apoptotic signaling pathway and response to oxidative stress, as well as signaling pathways such as cell apoptosis and regulation of TNF were closely related to common target genes. Molecular docking results also showed kaempferol has good binding properties with predicted targets Bcl-2, BAX, and CASP3. Compared with the model group, the pathological changes of cartilage in the kaempferol group were significantly reduced, OARSI scores were significantly decreased (P<0.001), and cartilage area was increased (P<0.01). In addition, Western blot analysis showed that kaempferol significantly decreased the protein expression of BAX and CASP3 (P<0.01, P<0.05), and increased the protein expression of BCL-2 (P<0.05). Conclusion: The treatment of KOA with kaempferol has the characteristics of multi-target and multi-pathway, and the mechanism may be related to the regulation of key genes such as Bcl-2, BAX, and CASP3 to inhibit cartilage apoptosis.
      原文链接:
    • 万方数据
    • 维普

    Expression and clinical significance of serum galactose lectin-2 and transforming growth factor-β2 in patients with atrial fibrillation

    Yang Hai-chenGeng Jia-yiLi Hao-yuXu Ning...
    26-31页
    查看更多>>摘要：Objective: To investigate the expression of serum transforming growth factor β2(TGF-β2) and galectin-2 (Galectin-2) in patients with atrial fibrillation and its clinical significance. Methods: A total of 150 inpatients in the Department of Cardiology of the First Affiliated Hospital of Bengbu Medical College from January 2019 to June 2019 were selected, including 84 patients in the paroxysmal atrial fibrillation group, 66 patients in the persistent atrial fibrillation group, and 120 patients with sinus rhythm excluding atrial fibrillation who were hospitalized during the same period were selected as the control group. Blood routine, biochemical routine and cardiac ultrasonic morphological examination were improved after admission. In addition, the serum levels of TGF-β2 and Galectin-2 in each group were detected by Elisa and Western Blot, and the differences and clinical significance of TGF-β2 and Galectin-2 in different groups were statistically analyzed. The correlation between TGF-β2, Galectin-2 and left atrial end-diastolic diameter (LAD) was further determined by Pearson correlation analysis. By means of binary logistics regression analysis, the factors affecting atrial fibrillation were obtained. Results: Compared with the control group, age and LAD in atrial fibrillation group were significantly increased (P<0.05). The serum concentrations of TGF-β2 and galectin-2 in persistent af group were significantly increased compared with those in paroxysmal af group and control group (P<0.05), while those in control group were significantly decreased compared with paroxysmal AF group (P<0.05). Pearson correlation analysis showed that TGF-β2 and Galectin-2 concentrations were significantly positively correlated with LAD size in the experimental group (P<0.05). Binary logistic regression analysis showed that TGF-β2, galectin-2 and LAD were risk factors for atrial fibrillation (P<0.05). Conclusion: TGF-β2 and Galectin-2 are closely related to the occurrence and development of atrial fibrillation. Both TGF-β2 and Galectin-2 were positively correlated with LAD. The three have a synergistic effect on the occurrence and progression of atrial inflammatory fibrosis in patients with ATRIAL fibrillation, jointly promoting mechanical and electrical remodeling of atrial, and can be used as risk factors to evaluate the occurrence and progression of atrial fibrillation.
      原文链接:
    • 万方数据
    • 维普

    Therapeutic effect of plaque psoriasis by fire acupuncture based on theory of midnight-noon ebb-flow

    XIAO XueYANG Su-qing
    32-38页
    查看更多>>摘要：Objective: To observe the clinical efficacy, immune inflammatory factors and hemorheology of patients with plaque psoriasis by using fire acupuncture combined with the theory of midnight-noon ebb-flow. Methods: Sixty-two patients with plaque psoriasis who met the diagnosis and inclusion criteria were randomly divided into control group and fire acupuncture with midnight-noon ebb-flow, with 31 cases in each group. The control group was treated with carpotriol ointment for external use, while the fire acupuncture group was treated with fire acupuncture with midnight-noon ebb-flow on the basis of the former and selected points by the opening method through midnight-noon ebb-flow theory. The patients in both groups were treated for 8 weeks and followed up for 4 weeks. The levels of PASI, DLQI, PSQI, HAMA, hs-CRP, TNF-α and hemorheology indexes in 2 groups before and after treatment were observed, including the comparison of whole blood viscosity, plasma viscosity and hematocrit. Results: PASI, DLQI, PSQI, HAMA, hs-CRP, TNF-α, blood viscosity and hematocrit levels were significantly improved after treatment (P < 0. 05, P < 0. 01), and the group in fire acupuncture with midnight-noon ebb-flow was significantly better than control group after treatment (P<0.01). Conclusion: fire acupuncture with midnight-noon ebb-flow can improve the sleep quality of patients with psoriasis, relieve anxiety and effectively improve the blood cell rheology and microcirculation, alleviate clinical symptoms and improve the quality of life.
      原文链接:
    • 万方数据
    • 维普

    Clinical characteristics of Danshen Ligustrazine injection in the treatment of coronary heart disease with hypertension-A real world study

    SUN Lin-xiGao YangXIE Yan-mingZhang Li-dan...
    39-46页
    查看更多>>摘要：Objective: To explore the characteristics and rules of Danshen Ligustrazine Injection in the treatment of coronary heart disease with hypertension. Methods: From the information systems of 12 tertiary tier-one hospitals across the country, we extracted the medical data of the application of Danshen Ligustrazine Injection in the treatment of patients with coronary heart disease and hypertension. After normalization, the model was established by Apriori algorithm, and the association rules were analyzed by Clementine 12.0 software. Results: Most of the 1928 patients were between 75 and 90 years old (54.26％). There were more males than females, most with type 2 diabetes, cerebral infarction, etc. Each dose was more than 10 mL (52.78％). Aspirin enteric-coated tablets (67.63％), L-carnitine injection (58.77％), and atorvastatin calcium capsules (50.93％) were often used in combination with safflower yellow pigment (22.20％), Shexiang Baoxin Pill (16.55％), Suxiao Jiuxin Pill (15.09％); the most commonly used combination of western medicine was anticoagulant thrombolytic and antiplatelet drugs (85.84％), and the type of Chinese medicine was Huoxuehuayu (72.98％); The most common combination of two western medicines was L-carnitine injection+aspirin enteric-coated tablets, with a support of 41.9％; The most common combination of two Chinese and western medicines is western medicine·anti-anginal medicine + western medicine · anticoagulant thrombolytic and antiplatelet drugs with a support of 67.6％. Conclusion: Danshen Ligustrazine injection is mainly used in elderly patients with coronary heart disease and hypertension, with many comorbidities. The dosage standard needs to be optimized. The combination of drugs and guidelines should coordinate with each other, which provide clues for clinical diagnosis and treatment and optimization of medication.
      原文链接:
    • 万方数据
    • 维普

    Clinical efficacy and perioperative safety of simultaneous or staged bilateral total hip arthroplasty:A Meta analysis

    XIONG Bing-langLIN Tian-yeYANG PengXU Jing-li...
    47-55页
    查看更多>>摘要：Objective: To investigate the clinical efficacy and perioperative safety of simultaneous or staged bilateral total hip arthroplasty (THA) using meta-analysis. Methods: Relevant retrieval methods were developed to retrieve PubMed, Embase, Cochrane Library, CNKI, Wanfang and VIP databases, and the time was set to build the database until September 2020. All the literatures related to simultaneous or staged total hip arthroplasty were screened out. The final included literatures were determined according to the inclusion and exclusion criteria, and the quality of the literatures was evaluated. Meta analysis of all indexes was performed using Review Manager 5.3 software. Results: A total of 22 articles were included, including 3415 patients. Meta analysis results showed that the total hospitalization time in the simultaneous group was significantly lower than that in the staged group [MD=-9.99,95％CI(-14.72, -5.26), P<0.0001].Total hospitalization expenses in the simultaneous group were lower than those in the staged group [MD=-1.3, 95％CI (-1.73, -0.86), P<0.00001]. The operative time in the simultaneous group was less than that in the staged group [MD=-27.08, 95％CI (-40.89, -13.26), P=0.0001]. The total blood loss in the simultaneous group was less than that in the staged group [MD=-176.55, 95％CI (-282.5, -70.6), P=0.001]. The surgical transfusion volume in the simultaneous group was higher than that in the staged group [MD=69.85, 95％CI (40.48, 99.22), P<0.00001]. Postoperative Harris score of hip joint in the simultaneous group was higher than that in the staged group [MD=1.79, 95％CI(0.8, 2.79), P=0.0004]. The incidence of operative complications in the simultaneous group was lower than that in the staged group [OR=0.73, 95％CI (0.56, 0.96), P<0.05]. Conclusion: Compared with staged bilateral total hip replacement, simultaneous bilateral total hip replacement has advantages in terms of reducing hospitalization time, saving hospitalization expense, shortening operation time and reducing surgical bleeding amount, and can promote postoperative hip function recovery, reduce postoperative complications, but increase total blood transfusion volume.
      原文链接:
    • 万方数据
    • 维普

    Efficacy and prognosis of vacuum‑assisted excision for benign intraductal papilloma of breast: A meta‑analysis

    SU Ya?jingWU Huan?liangCHEN Yun?jingHE Gui?sheng...
    56-63页
    查看更多>>摘要：Objective: To evaluate the safety and effectiveness of vacuum‑assisted excision for benign intraductal papilloma of breast. Methods: The databases including PubMed, Embase, Cochrane Library, WangFang, CNIK, VIP and Web of Science were searched by computer, according the inclusion and exclusion criteria after screening of literature. The Cochrane handbook and Newcastle‑Ottawa scale was used to evaluate the studies. Then the Review Manager 5.3 software was to used analyze the data.Results: A total of 1016 patients was included in 9 articles. The amount of blood loss in vacuum‑assisted surgery was less than that in open surgery [MD=-6.38,95％CI(-9.90,-2.86),P<0.05]. The drainage[MD=-2.56,95％CI(-4.97,-0.15 ,P<0.05] and drainage time[MD=-0.25,95％CI(-0.40,-0.09),P<0.05] in vacuum‑assisted surgery was less than that in open surgery. There were also few postoperative complications than that in open surgery [ RR=0.43,95％CI(0.29,0.64),P<0.05]. Compared with open surgery, the postoperative recurrence rate was lower in vacuum‑assisted surgery [RR=0.26,95％CI (0.14, 0.49), P<0.05]. Last,there was no statistically significant difference in the operative time between vacuum‑assisted surgery and open surgery,[SD=-12.82,95％CI (-25.70, 0.06),P=0.05].Conclusion: Compared with open surgery, vacuum‑assisted excision has the advantages of less blood loss and lower postoperative complications, but the operative time is not statistically significant compared with open surgery. In addition, compared with open surgery in this study, vacuum‑assisted excision has the advantages of lower recurrence rate, but it still needs long‑term dynamic observation.
      原文链接:
    • 万方数据

    Value of 18F‑FDG PET/CT in predicting EGFR mutation status and PD‑L1 expression in non‑small cell lung cancer

    LI Xue?yanWANG Da?weiYU Li?juanLIN Xiu?yan...
    64-70页
    查看更多>>摘要：Objective: To explore the relationship between multimodal imaging features and metabolic parameters derived from 18F‑FDG PET/CT and the mutation status of epidermal growth factor receptor (EGFR) and the expression of programmed cell death ligand 1 (PD‑L1) in non‑small cell lung cancer (NSCLC), so as to provide objective evidence for clinical screening of targeted therapy and immunotherapy beneficiaries. Methods: Data of NSCLC patients who underwent 18F‑FDG PET/CT scans, EGFR mutation and PD‑L1 expression tests with confirmed pathological results were collected. The differences of PET/CT morphological characteristics and metabolic parameters between the EGFR mutation and the wild group, and between the PD‑L1 expression positive and the negative group were analyzed. Univariate and multivariate logistic regression analyses were used to test the correlation between clinical and PET/CT parameters and EGFR mutation and PD‑L1 expression status. Results: MTV(P=0.01) and TLG(P=0.00) values of EGFR mutant patients were statistically lower than those of wild type patients. Univariate and multivariate logistic analysis showed that age(P=0.03), smoking history(P=0.00) and MTV(P=0.00) were independent risk factors for EGFR mutation. The positive expression of PD‑L1 in stage Ⅲ/Ⅳ group was significantly higher than that in stage Ⅰ/Ⅱ Group (P=0.01), but there was no significant difference in different age, gender, smoking history, SUVmax, MTV, TLG, LDH and CEA groups. Logistic univariate analysis showed that only stage was associated with the positive expression rate of PD‑L1 (P=0.01). There was no significant correlation between PET/CT parameters and PD‑L1 status. Conclusion: PET/CT metabolic parameters MTV, age and smoking history are independent predictors of EGFR mutation, which is expected to provide objective evidence for clinical screening of targeted treatment beneficiaries; however, PET/CT metabolic parameters are not good in predicting PD‑L1 protein expression, which needs to be further verified by large sample.
      原文链接:
    • 万方数据