首页期刊导航|海南医科大学学报（英文版）
期刊信息/Journal information
海南医科大学学报（英文版）
海南医科大学学报（英文版）

半月刊

海南医科大学学报（英文版）/Journal Journal of Hainan Medical University
正式出版
收录年代

    Research progress on the correlation between immunosuppressive population and SARS-CoV-2 variation

    Yu-Xia ZhouCai-Hong WangXiao-Wen YaoRong Wang...
    1-4页
    查看更多>>摘要：Recently, some severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants represented by B.1.7 (Alpha), B.1.351 (Beta), P1 (Gamma), B.1.617 (Delta) and B.1.529 (Omicron) have higher transmission and pathogenicity. Especially, Omicron causes global panic because of its high mutation in infectivity and vaccine escape, and their emergence makes the epidemic more difficult to control. The latest research reports indicate that these new corona mutants may first be mutated in the special population of immunosuppressive patients, and long-term infection and artificial immunotherapy may promote the variation of SARS-CoV-2. Not only that, there are a large number of congenital or acquired immunosuppressive patients worldwide, and immunosuppressive patients infected with SARS-CoV-2 will also have different impacts on social public health security. Therefore, we should pay more attention to the immunosuppressive population. This paper will briefly analyze the correlation between immunosuppressive population and SARS-CoV-2 variation in the form of review.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    The mechanism of Jiuyi powder and its effective components in inhibiting the M1 phenotype polarization of macrophages on chronic and refractory wounds infected by MRSA

    Yu-Lian YinTian MengLi-Na MaYi-Wei Fan...
    5-10页
    查看更多>>摘要：Objective: To observe the effect of Jiuyi powder and its active components on the bacterial culture and macrophage phenotypic factors of the chronic refractory wound rat model, and to explore its mechanism of removing decay and promoting muscle growth. Methods: SD rats were divided into control group, Jiuyi powder group, Shengdan group, and calcined gypsum group, with 8 rats in each group. MRSA-infected skin lesions and wounds were used to build a model of chronic and difficult-to-heal wounds in rats. After the model was formed, the control group was treated with daily routine nitrofural disinfection and replaced with sterile gauze. On the basis of the control group, quantitative Jiuyi powder, Shengdan powder, and calcined gypsum powder were used for dressing change, once a day for 7 consecutive days. Before and after the last administration, collect rat wound secretions for bacterial culture, inducible monoxide nitrogen synthase content. At the same time, after the last administration, the rat wound tissue was excised for histopathology and immunofluorescence double staining to label macrophages and their M1 phenotype. Results: After the last dressing change, the wound healing of Jiuyi powder group was better than the other groups, and the wound healing rate of each group had significant difference (P <0.05). The histomology showed that the inflammation of Jiuyi powder group was controlled and had a healing trend.After the last drug change, the contents of TNF-α, IL-6 and iNOS in serum of all groups decreased, and the contents of IL-6, TNF-α and iNOS in serum of Jiuyi powder group decreased significantly before and after medication (P<0.05).There was statistical significance in serum IL-6 content between calcined gypsum group and Shengdan group before and after medication (P<0.05).In addition, the results showed that the contents of IL-6 and iNOS in serum of Jiuyi powder group were statistically different from those of the control group (P<0.05).Tissue immunofluorescence double staining showed that the positive rate of M1 macrophages in Jiuyi powder group and Shengdan group was significantly lower than that in control group (P< 0.05). The MRSA negative conversion rate of Jiuyi powder group and Shengdan group was better than that of the control group and calcined gypsum group (P<0.05). Conclusion: Jiuyi powder can improve the inflammation of chronic refractory wounds, and has antibacterial, anti-corrosion and myogenic effects. Its mechanism may be related to the inhibition of macrophage M1 phenotype polarization.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Optimization of extraction technology of safflower polysaccharide based on central composite design-response surface methodology

    Rui WangYa-Lan LiHao-Tian BaiJing Yang...
    11-17页
    查看更多>>摘要：Objective: To explore the best extraction technology of safflower polysaccharide by central composite design response surface methodology and evaluate its quality. Methods: Taking the extraction rate of Carthamus tinctorius polysaccharide as the index, taking the ratio of solid to liquid, extraction time, extraction temperature and extraction times as the investigation factors, based on the single factor experiment, the central composite design-response surface methodology was used to optimize the optimum extraction process of Carthamus tinctorius polysaccharide and verify it. Results: The response surface model was established with the extraction rate of Carthamus tinctorius polysaccharide as dependent variable Y, the ratio of solid to liquid, extraction time and extraction temperature as independent variables X, P<0.0001. The optimum extraction process was as follows: the ratio of solid to liquid was 1: 16.69, the extraction temperature was 91.39℃, and the extraction working time was 89.78min. Under these conditions, the extraction rate of safflower polysaccharide can reach 7.45％, The experimental results show that RSD is 1.05％,and the model can well predict the experimental results. Conclusion: Central Composite Design-Response Surface Methodology has the advantages of high extraction rate, simple, effective and reasonable process operation, high stability and high precision, which can be fully applied to the resource management and utilization of safflower polysaccharide.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Mechanism of calcium hydroxide inhibiting methicillin resistant Staphylococcus aureus

    Zheng-Dong GuoYang-Yang BianXiao-Qian LiuDong Wang...
    18-22页
    查看更多>>摘要：Objective:To investigate the inhibitory effect of calcium hydroxide on methicillin-resistant Staphylococcus aureus and the related inhibition mechanism. Methods: To determine the minimum inhibitory concentration of calcium hydroxide using microplate dilution method; to compare the effects of calcium hydroxide at 8MIC, MIC, 1/4MIC and 0 concentrations on MRSA using growth curve method; to determine the effects of calcium hydroxide on the cell membrane of methicillin-resistant Staphylococcus aureus using calcium xanthophyll and propidium iodide fluorescence staining The effect of calcium hydroxide on the morphology of methicillin-resistant Staphylococcus aureus was observed by scanning electron microscopy; the inhibition mechanism of calcium hydroxide on MRSA was investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis of protein bands. Results: The MIC of calcium hydroxide on MRSA was 3.125 mg/mL; the fluorescence intensity showed significant changes after co-culture of calcium hydroxide with bacteria; MRSA appeared to be significantly crumpled and broken in the presence of calcium hydroxide; the SDS-PAGE experimental bands indicated that the protein inside the bacteria decreased accordingly with the increase of calcium hydroxide concentration. Conclusion: Calcium hydroxide has a significant inhibitory effect on the growth of MRSA, and its bactericidal mechanism may be related to the destruction of bacterial body structure.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Comparison of the curative effect of intracoronary retrograde thrombolysis and thrombus aspiration combined with stent implantation on STEMI patients

    Yan-Zi LinTian-Fa LiYu-Zhuo ZhangYi-Ting Chen...
    23-28页
    查看更多>>摘要：Objective: To evaluate the short-term and long-term curative effects of precise intracoronary retrograde thrombolysis combined with stent implantation, thrombus aspiration combined with stent implantation, and traditional stent implantation in patients with acute ST-segment elevation myocardial infarction. Methods: From January 2018 to October 2019, 184 patients diagnosed with acute ST-segment elevation myocardial infarction and infarction-related arterial blood flow TIMI 0 grade in the First Affiliated Hospital of Hainan Medical College and percutaneous coronary intervention(PCI) were selected. According to different surgical methods, patients were divided into intracoronary retrograde thrombolysis combined with stent implantation group (thrombolysis group, n=57 cases), thrombus aspiration combined with stent implantation group (aspiration group, n=57 cases) ), traditional stent implantation group (traditional group, n=70 cases). Compare the incidence of no-reflow phenomenon after percutaneous coronary intervention, the rate of 1 hour ST_segment fall≥50％ in the ECG after PCI, and the main adverse cardiovascular events(MACE) during hospitalization; compare the left ventricular end-diastolic diameter(LVEDD) 、left ventricular ejection fraction(LVEF)and major adverse cardiovascular events at 1 year after PCI. Results: 1. The short-term effects:The incidence of no-reflow phenomenon in the thrombolytic group was lower than that of the aspiration group and the traditional group, and the rate of 1hSTR≥50％ was higher than that of the aspiration group and the traditional group, the difference was statistically significant (P<0.05). 2. The long-term effects:1 year after percutaneous coronary intervention, the LVEDD of the thrombolytic group was lower than that of the aspiration group and the traditional group, while the LVEF was higher than the aspiration group and the traditional group, and the incidence of major adverse cardiovascular events in the thrombolytic group at was lowest,both the difference was statistically significant ( P<0.05). Conclusion: The application of intracoronary retrograde thrombolysis combined with stent implantation in STEMI patients can reduce the occurrence of no-reflow, improve long-term cardiac function, and reduce the occurrence of major adverse cardiovascular events for short-term and long-term.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Significance of preoperative fibrinogen levels, neutrophils-to-lymphocyte ratio and its combined scores in evaluating the prognosis of postoperative patients with gastric cancer

    Jian-Zhong DengXiu-Ling LiuQian LiuWen-Jing Li...
    29-33页
    查看更多>>摘要：Objective:To investigate the clinic significance of the combined detection of peripheral fibrinogen (Fbg) and neutrophils-to-lymphocyte ratio (NLR) (F-NLR) on the prognosis of postoperative gastric cancer patients. Methods: The clinical and pathological characteristics of 70 gastric cancer patients who were diagnosed by gastroscopy and surgically resected were collected, and the relationship between Fbg, NLR and F-NLR scores and pathological characteristics and prognosis of gastric cancer patients was retrospectively analyzed. Results: There were statistically significant differences in the levels of NLR in gastric cancer patients of different genders (P < 0.05), while there were no significant differences in the levels of NLR and Fbg in other pathological factors such as age, T stage, lymph node metastasis and TNM stage(P > 0.05). The f-NLR score of gastric cancer patients with different gender, T stage and TNM stage had statistical significance (P < 0.05), but there was no statistical significance in the F-NLR score of gastric cancer patients with different age and whether lymph node metastasis (P > 0.05). Univariate analysis showed that Fbg, NLR, F-NLR score, and TNM stage had an effect on recurrence and survival of patients with gastric cancer after surgery (P <0.05); multivariate COX regression analysis showed that only F-NLR score and TNM stage were independent risk factors for relapse and survival of patients with gastric cancer (P <0.05). Conclusion: The F-NLR scores about the combined detection of Fbg and NLR may guide clinical prediction of the prognosis of gastric cancer patients.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Analysis of the law of traditional Chinese medicine in prevention and treatment of gastrointestinal reaction caused by lung cancer chemotherapy

    Yue-Jie LinYue LuoLu-Yao WangPei-Tong Zhang...
    34-40页
    查看更多>>摘要：Objective: To explore the law of traditional Chinese medicine in prevention and treatment of gastrointestinal reaction after chemotherapy for lung cancer. Methods: The literatures on prevention and treatment of gastrointestinal reaction after chemotherapy with Chinese medicine were searched from CNKI, Wanfang and VIP databases from the establishment of the database to September 2020. After screening, the database was established, and data mining was conducted by using IBM SPSS statistics 20.0 and IBM SPSS modeler 18.0 to summarize the medication rules of traditional Chinese medicine in the prevention and treatment of gastrointestinal reaction after chemotherapy for lung cancer. Results: (1) A total of 131 literatures involving 245 traditional Chinese medicines were included for data mining; (2) High frequency drugs include Astragalus membranaceus, liquorice, Poria cocos, Atractylodes macrocephala, Pinellia ternata, Codonopsis pilosula, Ophiopogon japonicus, coix seed, Pericarpium Citri Reticulatae, Radix Glehniae and so on; (3)The traditional Chinese medicine used is mainly tonifying deficiency medicine, resolving phlegm and relieving cough and asthma medicine, diuresis and dampness medicine, heat clearing medicine and antiphlogistic medicine. The medicine tastes bitter and sweet, and has cold and warm properties. The meridian tropism involves liver, lung, stomach, spleen and kidney;(4) Seven core TCM combinations were obtained by cluster analysis, and 24 drug pairs (groups) were obtained by association rule analysis. Conclusion: Through the data mining of traditional Chinese medicine for the prevention and treatment of gastrointestinal reaction after chemotherapy for lung cancer, we can find out the potential rules of traditional Chinese medicine prescription, and clarify the core Chinese medicine, which is helpful to provide certain reference for the development and research of drugs for the treatment of gastrointestinal reaction and modern clinical practice.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Study on the effect of Danbei Yifei formula on pulmonary fibrosis based on network pharmacology and molecular docking technology

    Xiao-Jun CaiBai-Hua JiangZhen-Hua LuTao Wang...
    41-46页
    查看更多>>摘要：Objective: To determine the pharmacodynamic material basis and mechanism of Danbei Yifei formula on pulmonary fibrosis. Methods: Starting with the clear absorbed components of Danbei Yifei formula or the potential effective components in line with the five rules of Ribinsky, the network pharmacology method and technology of traditional Chinese medicine were used to predict and analyze the action targets of Danbei Yifei formula in vivo, such as Salvia miltiorrhiza, PINBEI, Taoren, etc. On the basis of enrichment analysis, the core pathway of Danbei Yifei formula in the treatment of pulmonary fibrosis was identified, and the binding energy of drug ligand and protein target was determined through molecular docking technology simulation and verification, and its affinity and stability were evaluated. To clarify the material basis and mechanism of Danbei Yifei formula in the treatment of pulmonary fibrosis. Result: The results of network pharmacology prediction of traditional Chinese medicine showed that Danbei Yifei formula contained 72 potential pharmacodynamic components and 26 corresponding targets, including CHRM1、MAPK14、CCL2、ADRB1、PTGS1、PPARG、ALOX5、Pde3a、CHRM2、Adrb2、TNF、JUN、Adora2a、LTA4H、CYP1A2、OPRD1、CHRM3、DRD2、OPRM1、ARG1、EDNRA、Il6st、TACR1、MMP1、MMP8、Ptgs2, which were related to pulmonary fibrosis and pulmonary fibrosis Lung related diseases are highly correlated. There were 26 Go items (P < 0.05) in go functional enrichment analysis, including 22 biological process (BP), 9 cellular component (CC) and 3 molecular function (MF) categories. The results of network pharmacology showed that many components, such as protocatechuic acid and aminosuccinic acid, had direct effects on known targets of pulmonary fibrosis. Conclusion: Danbei Yifei formula contains many effective components which have inhibitory effect on pulmonary fibrosis, and it may play its role through the mechanism of multi-component and multi-target synergistic effect.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Astragalus injection for asthma: A meta-analysis and systematic review

    Le BaiYong XuLi ZhangXian-Mei Zhou...
    47页
    查看更多>>摘要：Objective: To evaluate the efficacy of Astragalus Injection on asthma. Methods: CNKI, VIP, Wan Fang Database, Medline, Embase, Web of Science and Cochrane Library were searched for published researches up to November, 2020. Randomized controlled trials that focused on Astragalus Injection for asthma were included. We managed the data analysis with RevMan 5.3 software. Results: A total of 17 RCTs with 1648 patients were involved in the meta-analysis which indicated that Astragalus Injection could improve clinical efficacy[OR = 4.72, 95％CI(3.08,7.24), P < 0.00001] and improve FEV1[MD = 0.48, 95％CI(0.32,0.64), P < 0.00001], while reduce IL-4[MD = -6.29, 95％CI(-8.91,-3.66), P < 0.00001], IL-6[MD = -18.46, 95％CI(-23.17,-13.75), P < 0.00001] and IL-17[MD = -3.14, 95％CI(-5.51,-0.76), P < 0.00001] and decrease adverse events[OR = 0.18, 95％CI(0.08,0.43), P = 0.0001]. Conclusion: Astragalus Injection is more effective and of safety, but more RCTs are needed.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    A systematic review and meta-analysis on the protective effect of combined TCM and western medicine on cardiotoxicity induced by anthracycline chemotherapy

    Can LiuYan-Wei XingXin SuXu Liu...
    48页
    查看更多>>摘要：Objective: To systematically evaluate the therapeutic effect of TCM on cardiotoxicity induced by anthracycline chemotherapy to provide clinical guidance. Methods: China hownet database (CNKI), Chinese biomedical literature retrieval (SinoMed), ten thousand Data knowledge service system (WanFang Data), VIP full-text database (VIP), PubMed, MedLine and the cochrane library were searched from inception to 1st, December,2020. Relevant combined TCM and Western medicine on cardiotoxicity randomized controlled trials (RCTs) were collected. The risk of bias in included RCTs were evaluated according to the Cochrane Handbook. The required indicators were extracted and included in RevMan5.4 analysis. Results: A total of 2844 patients were included in 37 RCTs. The results of meta-analysis showed that compared with the control group, TCM treatment group could alleviate the effect of anthracycline on ventricular ejection function, reduce the effect of anthracycline chemotherapy drugs in lowering left ventricular ejection fraction (WMD=6.44,95％CI 0.38 to 12.50 ,P=0.04), reduce the effect of anthracycline chemotherapy in increasing left ventricular end diastolic diameter (WMD=-0.59,95％CI -0.74 to -0.44 ,P<0.00001), reduce the effect of anthracycline chemotherapy drugs in increasing left ventricular end systolic diameter (WMD=-0.47,95％CI -0.60 to -0.34 ,P<0.00001), and reduce the effect of anthracyclines on troponin I (WMD=-0.19,95％CI -0.23 to -0.16 ,P<0.00001),troponin T (WMD=-0.02,95％CI -0.03 to -0.01 , P<0.00001) and brain natriuretic peptide (WMD=-32.21,95％CI -58.21 to -6.22 ,P=0.02). Conclusion: The existing evidence can prove that TCM has a protective effect on cardiotoxicity caused by anthracycline chemotherapy, maintaining left ventricular ejection fraction and ventricular diameter, controlling the level of myocardial injury markers, and alleviating cardiac injury.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普