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绿色化学工程（英文）
绿色化学工程（英文）
绿色化学工程（英文）/Journal Green Chemical EngineeringCSCD
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收录年代

    Biomanufacturing boosts the high-level development of economy and society

    Chun LiAn-Ping ZengYing-Jin Yuan
    135-136页
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Enzyme annotation for orphan reactions and its applications in biomanufacturing

    Zhen ZhangChun Li
    137-145页
    查看更多>>摘要：There are lots of biochemical reactions in the biosynthetic pathway without associated enzymes.Reactions predicted by retro-biosynthetic tools are not assigned gene sequences.Besides,non-natural reactions designed with novel functions also lack suitable enzymes.All these reactions can be categorized as orphan reactions.The absence of protein-encoding genes in these orphan reactions limits their direct experimental implementation.Computational tools have been developed to find candidate enzymes for these orphan reactions.Herein,we discuss recent advances in these computational tools,including reaction similarity-based methods for calculating the substructural similarity between orphan reactions and known enzymatic reactions;sequence-based tools combine metabolic knowledge base and phenotypic information with genomic,transcriptomic,and metabolomic data to mine appropriate enzymes for orphan reactions;and approaches based on the creation of enzyme variants for orphan reactions as enzyme engineering modifications and de novo design of enzymes.We believe that our review will greatly facilitate the design of microbial cell factories and contribute to the development of the biomanufacturing field.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Metabolic flux simulation of microbial systems based on optimal planning algorithms

    Chen YangBoyuan XueYiming ZhangShaojie Wang...
    146-159页
    查看更多>>摘要：The genomic scale metabolic networks of the microorganisms can be constructed based on their genome se-quences,functional annotations,and biochemical reactions,reflecting almost all of the metabolic functions.Mathematical simulations of metabolic fluxes could make these functions be visualized,thereby providing guidance for rational engineering design and experimental operations.This review summarized recently devel-oped flux simulation algorithms of microbial systems.For the single microbial systems,the optimal planning algorithm has low complexity because there is no interaction between microorganisms,and it can quickly simulate the stable metabolic states through the pseudo-steady hypothesis.Besides,the experimental conditions of single microbial systems are easier to reach or close to the optimal states of simulation,compared with pol-ymicrobial systems.The polymicrobial culture systems could outcompete the single microbial systems as they could relieve metabolic pressure through metabolic division,resource exchange,and complex substrate co-utilization.Besides,they provide varieties of intracellular production environments,which render them the po-tential to achieve efficient bioproduct synthesis.However,due to the quasi-steady hypothesis that restricts the simulation of the dynamic processes of microbial interactions and the algorithm complexity,there are few re-searches on simulation algorithms of polymicrobial metabolic fluxes.Therefore,this review also analyzed and combed the microbial interactions based on the commonly used hypothesis of maximizing growth rates,and studied the strategies of coupling interactions with optimal planning simulations for metabolism.Finally,this review provided new insights into the genomic scale metabolic flux simulations of polymicrobial systems.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Bioconversion of lignocellulosic biomass into bacterial nanocellulose:challenges and perspectives

    Wenchao LiYuqing ShenHuan LiuXinxin Huang...
    160-172页
    查看更多>>摘要：Nanocellulose has various outstanding properties and great potential for replacing petrochemical products.The utilization of lignocellulose to produce nanocellulose is of great significance to the sustainable development of the economy and society.However,the direct extraction of nanocellulose from lignocellulose by chemical method is challenged by toxic chemicals utilization,energy and time consumption,and waste water generation.Therefore,this paper addressed the conversion of lignocellulosic biomass into bacterial nanocellulose(BNC)by the biological method.Moreover,this article highlights the recent advances in potentials and challenges of lignocellulosic biomass for BNC production through the bioconversion process,including biomass pretreatment,enzymatic hydrolysis,glucose and xylose fermentation,GA accumulation,and inhibitor tolerant.The development in metabolic and evolutionary engineering to enhance the production capacity of BNC-producing strain is also discussed.It is expected to provide guidance on the effective bioproduction of nanocellulose from lignocellulosic biomass.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Multi-functional engineered polypeptide-based drug delivery systems for improved cancer therapy

    Xiaobin LiJunyu LiuHaihong ChenYaxin Chen...
    173-188页
    查看更多>>摘要：With the rapid development of chemical engineering and biotechnology,polypeptide,as a promising candidate in the biomedical field,has been thoroughly investigated and extensively used as the drug delivery vehicle for diseases treatment,especially cancer,owing to the high biocompatibility,good biodegradability,versatile con-structions,and diverse functions.Engineered polypeptide-based drug delivery system(so-called EPP-DDS)can deliver the cargos to the target site via a specific recognition effect,followed by overcoming the barriers like blood brain barrier(BBB)and releasing them by responding to the microenvironment cues,to improve the therapeutic efficacy and reduce the side-effect.Herein,it's of great importance to conclude and summarize the updates on EPP-DDS developed by chemical engineering methods.In this review,we first summarized the recent updates in the manufacturing of polypeptide and preparation of EPP-DDS based on green biochemical engineering and/or synthetic processes for cancer therapy,including chemotherapy,immunotherapy,photodynamic therapy(PDT),gene therapy,and combination therapy.Then,we surveyed the research progress of inflammation-mediated cancer treatment strategies based on EPP-DDS with high anti-inflammation activity.Finally,we concluded the discovery and green production process of engineered polypeptide,challenges,and perspectives of EPP-DDS.Overall,the EPP-DDS has great potential for cancer therapy in the clinic with improved therapeutic efficacy and reduced adverse effect,which needs the innovation of green biochemical engineering for customized design and production of polypeptides.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Application and progress of techno-economic analysis and life cycle assessment in biomanufacturing of fuels and chemicals

    Rongzhan FuLixia KangChenyue ZhangQiang Fei...
    189-198页
    查看更多>>摘要：To reduce the dependency on petroleum-based products and emission of greenhouse gas,renewable biofuels and chemicals play an important role to meet the unmatched energy demands of the rapidly growing population.However,most biofuel and chemical products do not reach the commercialization stage,mainly hindered by incomparable economics to petroproducts.Techno-economic assessment(TEA)is a useful tool to estimate eco-nomic performance,and identify bottlenecks for the development of biofuel and chemical production technology,meanwhile,life cycle assessment(LCA)is applied to assess sustainability by reducing the environmental impact of biofuel and chemical production.This present review covers TEA and LCA research progress in the manufacturing of biofuels and biochemical,and discusses the impacts of TEA and LCA results on the development and optimi-zation of biofuel and chemical production.In addition,challenges associated with TEA and LCA of biofuel and biochemical production were briefly overviewed,and potential approaches that may overcome such challenges were discussed enabling viable and sustainable biomanufacturing of fuels and chemicals.Future integrated TEA and LCA studies could significantly promote the economic and sustainable development of the biomanufacturing process.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Engineering the native methylotrophs for the bioconversion of methanol to value-added chemicals:current status and future perspectives

    Jing WangRuirui QinYuanke GuoChen Ma...
    199-211页
    查看更多>>摘要：Methanol is becoming an attractive fermentation feedstock for large-scale bioproduction of chemicals,due to its natural abundance and mature production technology.Native methylotrophs,which can utilize methanol as the only source of carbon and energy,are ideal hosts for methanol bioconversion due to their high methanol utili-zation rate and have been extensively employed in the production of value-added chemicals from methanol.Here,we review the natural methanol utilization pathways in native methylotrophs,describing the available synthetic biology tools developed for engineering native methylotrophs,and discuss the strategies for improving their methanol utilization efficiency.Finally,the representative examples of engineering the native methylotrophs to produce value-added products from methanol are summarized.Furthermore,we also discuss the major challenges and possible solutions for the application of native methylotrophs in methanol-based biomanufacturing.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Highly selective kinetic resolution of D/L-syn-p-sulfone phenylserine catalyzed by D-threonine aldolase in two-phase ionic solvent

    Fengfan LiuZhihao ShiJinmei ZhuXiaobin Liang...
    212-216页
    查看更多>>摘要：In the chemical synthesis of L-syn-p-methylsulfoxide phenylserine ethyl ester(D-ethyl ester),L-tartaric acid or enzymatic resolution is employed to resolve the racemate,and thus obtain the target compound,and the remaining isomer can be recycled to obtain the raw material.In this study,high-purity L-syn-p-methylsulfoxide phenylserine(L-syn-MPS)was obtained.The kinetics of the D-threonine aldolase enzymatic hydrolysis reaction reveals that D-syn-p-sulfoxylphenylserine resolves well in[BMIM][BF4]ionic solvents.The D/L-syn-MPS racemate was resolved using a two-phase ionic solvent[BMIM][NTf2]to afford L-syn-MPS(ee(enantiomeric excess)＞99％)and a white solid in 41.7％yield.Therefore,this system is suitable for the separation of insoluble aldehydes and successfully avoids the condensation of hydroxyl aldehydes to form D-anti-MPS.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Engineering Escherichia coli for high-yield production of ectoine

    Daoan WangJiamin ChenYang WangGuocheng Du...
    217-223页
    查看更多>>摘要：Ectoine is a natural macromolecule protector and synthesized by some extremophiles.It provides protections against radiation-mediated oxidative damages and is widely used as a bioactive ingredient in pharmaceutics and cosmetics.To meet its growing commercial demands,we engineered Escherichia coli strains for the high-yield production of ectoine.The ectABC gene cluster from the native ectoine producer Halomonas elongata was intro-duced into different Escherichia coli(E.Coil)strains via plasmids and 0.8 g L-1 of ectoine was produced in flask cultures by engineered E.coli BL21(DE3).Subsequently,we designed the ribosome-binding sites of the gene cluster to fine-tune the expressions of genes ectA,ectB,and ectC,which increased the ectoine yield to 1.6 g L-1.After further combinatorial overexpression of Corynebacterium glutamicum aspartate kinase mutant(G1A,C932T)and the H.elongate aspartate-semialdehyde dehydrogenase to increase the supply of the precursor,the titer of ectoine reached to 5.5 g L1 in flask cultures.Finally,the engineered strain produced 60.7 g L1 ectoine in fed-batch cultures with a conversion rate of 0.25 g/g glucose.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Ionic-microenvironment stabilizes the disulfide engineered lysine decarboxylase for efficient cadaverine production

    Zhuang LiYaju XueXiuling JiYuhong Huang...
    224-232页
    查看更多>>摘要：Cadaverine is the key monomer for the synthesis of nylon 5X.Efficient and alkaline stable lysine decarboxylases are highly desirable for cadaverine production as the reaction pH increasing from 6.3 to 8.5.However,the most studied lysine decarboxylase CadA(E.coli)lost almost all activity at pH 8.0,which is the foremost challenge for the industrial-cadaverine production.In this study,we first found that the Na+-microenvironment significantly improved the alkaline stability of the disulfide engineered lysine decarboxylase △LdcEt3(P233C/L628C)(half-life 362 h),compared to the conventional buffer(half-life 0.66 h)at pH 8.0.Meanwhile,the whole-cell conversion efficiency of the industrial-grade L-lysine with △LdcEt3 could reach up to 99％in 2 h in the fermenter.Experi-mental investigation and molecular dynamics confirmed that Na+-microenvironment could improve active-aggregation state and affect secondary structure of △LdcEt3.Therefore,Na+-microenvironment stabilizes △LdcEt3 providing a great potential industrial application for high-level cadaverine production.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普