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中国海洋大学学报（自然科学英文版）
中国海洋大学学报（自然科学英文版）

文圣常（院士）

季刊

1672-5182

xbywb@ouc.edu.cn

0532-82032408

266003

山东省青岛市鱼山路5号

中国海洋大学学报（自然科学英文版）/Journal Journal of Ocean University of ChinaCSCDCSTPCD北大核心SCI
查看更多>>创刊宗旨：我刊及时反映和报道海洋科学与工程、海洋环境科学与工程、海洋气象、海洋药物、海洋遥感、海洋信息系统与管理工程、水产、湖沼等领域的最新研究成果。论文栏目设置：研究论文、技术报告、研究快报、高新技术与信息跟踪、特约评介、综述等。读者对象：国内海洋及相关学科领域的科技人员、教师和管理人员，主要是国际海洋科学界的同行，以英语为语言工具的读者。
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    Identification,Characterization,and Probiotic Potentials of Lactobacillus pentosus SF-1

    JIAO LinLIANG ShufeiCHU RuixiaWANG Wentao...
    509-517页
    查看更多>>摘要：In recent years probiotics have been considered as a potential substitution of antibiotics to control pathogens and treat in-fectious diseases in aquaculture.In the present study a strain of Lactobacillus pentosus,named as L.pentosus SF-1,was isolated from waters in aquaculture.The species identification of this strain was conducted by 16S rRNA sequence,and the physiological and bio-chemical characteristics of this strain were assessed.Furthermore,the virulence,antibiotic sensitivity,cell surface characteristics and acid/base-resistance of L.pentosus SF-1 were determined to evaluate the probiotic potentials of this strain.Specifically,L.pentosus SF-1 is sensitive to most common antibiotics,and no hemolysin was generated from it,indicating the safety of this strain to hosts.In addition,L.pentosus SF-1 was able to tolerate the artificial gastric juice at pH 3 for 4h and the artificial intestinal fluid at pH 6.8 or 8.0 for 6h.Moreover,the analysis of self-aggregation and the adhesion of L.pentosus SF-1 to organic solvents suggested a high po-tential of L.pentosus SF-1 to inhabit the hosts,which was confirmed by testing the colonization of L.pentosus SF-1 in germ-free zeb-rafish.Interestingly,L.pentosus SF-1 displayed a high bactericidal activity against several bacterial pathogens.Consistently,the incu-bation of L.pentosus SF-1 significantly promoted the expression of antimicrobial components in zebrafish,contributing to the protec-tion of the fish from E.tarda infection in vivo.Taken together,the probiotic strain L.pentosus SF-1 could be applied as anti-infection reagent in aquaculture.
      原文链接:
    • 万方数据
    • 维普

    Non-Targeted Metabolomics Reveals the Metabolic Alterations in Response to Artificial Selective Breeding in the Fast-Growing Strains of Pacific Oyster

    HU BoyangTIAN YuanLIU ShikaiLI Qi...
    518-528页
    查看更多>>摘要：Pacific oyster(Crassostrea gigas)is one of the most important mollusks cultured all around the world.Selective breed-ing programs of Pacific oysters in China is initiated since 2006 and developed the genetically improved strain with fast-growing trait.However,little is known about the metabolic signatures of the fast-growing trait.In the present study,the non-targeted metabolomics was performed to analyze the metabolic signatures of adductor muscle tissue in one-year old Pacific oysters from fast-growing strain and the wild population.A total of 7767 and 10174 valid peaks were extracted and quantified in ESI+and ESI modes,resulting in 399 and 381 annotated metabolites,respectively.PCA and OPLS-DA revealed that considerable separation among samples from fast-growing strain and wild population,suggesting the differences in metabolic signatures.Meanwhile,81 significantly different metabo-lites(SDMs)were identified in the comparisons between fast-growing strain and wild population,based on the strict thresholds.It was found that there were highly correlation and conserved coordination among these SDMs.KEGG enrichment analysis indicated that the SDMs were tightly related to pantothenate and CoA biosynthesis,steroid hormone biosynthesis,riboflavin metabolism,and arginine and proline metabolism.Of them,the CoA biosynthesis and metabolism,affected by pantetheine and pantothenic acid,might be important for the growth of Pacific oysters under artificial selective breeding.The study provides the comprehensive views of me-tabolic signatures in response to artificially selective breeding,and is helpful to better understand the molecular mechanism of fast-growing traits in Pacific oysters.
      原文链接:
    • 万方数据
    • 维普

    A Transcriptome Analysis of Neural Tissue of Litopenaeus vannamei After Acute Exposure to Alexandrium pacificum

    YANG HuijieHUANG XianghuMA YukunHU Zhangxi...
    529-538页
    查看更多>>摘要：Alexandrium pacificum(A.pacificum)is a typical paralytic shellfish poisonous dinoflagellate.Harmful algal blooms(HABs)caused by this species can bring serious environmental problems and economic losses to the aquaculture industry.In this study,tran-scriptome sequencing and analyses were performed on the neural tissue of Litopenaeus vannamei(L.vannamei)after acute exposure to A.pacificum disrupted solution for 72h,and differentially expressed genes(DEGs)were identified.The results showed that,compared with the control samples,300 DEGs were identified in the experimental group,of which 194 were up-regulated,and 106 down-re-gulated.The gene ontology(GO)functional enrichment analysis showed that DEGs were significantly enriched in the cortical cyto-skeleton organization,troponin complex,amylo-alpha-1,6-glucosidase and thymidine phosphorylase.Kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis found that DEGs were mainly enriched in the oxidative phosphorylation process,intercellular tight junctions and mitophagy.The results showed that the proteoglycans,signaling pathways,and various metabolic processes that re-gulate cell proliferation,differentiation,and apoptosis all played an essential role in the response of L.vannamei to A.pacificum toxins.
      原文链接:
    • 万方数据
    • 维普

    Salinity Acclimation Induces Reduced Energy Metabolism,Osmotic Pressure Regulation and Transcriptional Reprogramming in Hypotrichida Ciliate Gastrostyla setifera

    JI XinBI LupingZOU SongbaoLI Wenlu...
    539-549页
    查看更多>>摘要：Coastal and estuarine protists are frequently exposed to salinity undulation.While the tolerance and stress responses of microalgae to salinity have been extensively studied,there have been scarce studies on the physiological response of heterotrophic protists to salinity stressing.In this study,we investigated the physiological response of the heterotrophic ciliate Gastrostyla setifera to a salinity of 3,via a transcriptomic approach.The first transcriptome of genus Gastrostyla was obtained utilizing a group of manually isolated ciliate individuals(cells)and RNA-seq technique.The completeness of the transcriptome was verified.Differentially express-ed gene(DEG)analysis was performed among the transcriptomes of G.setifera acclimated in saline water(salinity 3)and those cul-tured in fresh water.The results demonstrated a significant alternation in gene transcription,in which the ciliate exhibits a transcript-tomic acclimation in responding salinity stressing.The up-regulated DEGs were enriched in the pathways of cytoskeleton proteins,mem-brane trafficking,protein kinases and protein phosphatases.These may represent enhanced functions of ion transport,stress response and cell protections.Pathways involved in energy metabolism and biosynthesis were markedly down-regulated,reflecting decreased cell activity.Particularly,we detected significantly down-regulated genes involved in several pathways of amino acid catabolism,which may lead to accumulation of amino acids in the ciliate cell.Amino acid could act as compatible solutes in the cytoplasm to maintain the osmotic balance in saline water.Overall,this work is an initial exploration to the molecular basis of the heterotrophic protist res-ponding to salinity stressing.The result sheds light on the mechanisms of enhancement of cell protection,reduction of cell activity,and osmotic pressure regulation in ciliates acclimated to salinity.
      原文链接:
    • 万方数据
    • 维普

    Kelp Fucoidans Facilitate Vascular Recanalization via Inhibiting Excessive Activation of Platelet in Deep Venous Thrombosis Model of Mouse

    SUN TaohuaLIU JieYAN TaishanCHEN Anjin...
    550-556页
    查看更多>>摘要：This study was carried out explore the mechanism underlying the inhibition of platelet activation by kelp fucoidans in deep venous thrombosis(DVT)mouse.In the control and sham mice,the walls of deep vein were regular and smooth with intact intima,myometrium and adventitia.The blood vessel was wrapped with the tissue and there was no thrombosis in the lumen.In the DVT model,the wall was uneven with thicken intima,myometrium and adventitia.After treated with fucoidans LF1 and LF2,the thrombus was dissolved and the blood vessel was recanalized.Compared with the control group,the ROS content,ET-1 and VWF content and the expression of PKC-β and NF-κB in the model were significantly higher(P＜0.05);these levels were significantly reduced following treatments with LF2 and LF1.Compared with H2O2 treated-HUVECs,combined LF1 and LF2 treatment resulted in significant decrease in the expression of PKC-β,NF-κB,VWF and TM protein(P＜0.05).It is clear that LF1 and LF2 reduces DVT-induced ET-1,VWF and TM expressions and production of ROS,thus inhibiting the activation of PKC-β/NF-κB signal path-way and the activation of coagulation system and ultimately reducing the formation of venous thrombus.
      原文链接:
    • 万方数据
    • 维普

    Research on the Relationship Between Environmental and Economic Coupling Systems in Bohai Bay Area Based on a Vector Autoregression(VAR)Model

    CAO HuiminWANG PingZHANG SurongXU Dongpo...
    557-566页
    查看更多>>摘要：This study analyzed the impact of land-based contaminants and tertiary industrial structure on economic development in the selected Bohai Bay area,China.Based on panel data spanning 2011-2020,a vector autoregressive(VAR)model is used to ana-lyze and forecast the short-run and long-run relationships between three industrial structures,pollutant discharge,and economic de-velopment.The results showed that the environmental index had a long-term cointegration relationship with the industrial structure economic index.Per capital chemical oxygen demand(PCOD)and per capita ammonia nitrogen(PNH3N)had a positive impact on delta per capita GDP(dPGDP),while per capita solid waste(PSW),the secondary industry rate(SIR)and delta tertiary industry(dTIR)had a negative impact on dPGDP.The VAR model under this coupling system had stability and credibility.The impulse re-sponse results showed that the short-term effect of the coupling system on dPGDP was basically consistent with the Granger causa-lity test results.In addition,variance decomposition was used in this study to predict the long-term impact of the coupling system in the next ten periods(i.e.,ten years).It was found that dTIR had a great impact on dPGDP,with a contribution rate as high as 74.35％in the tenth period,followed by the contribution rate of PCOD up to 3.94％,while the long-term contribution rates of PSW,SIR and PNH3N were all less than 1％.The results show that the government should support the development of the tertiary industry to main-tain the vitality of economic development and prevent environmental deterioration.
      原文链接:
    • 万方数据
    • 维普