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中国海洋大学学报(自然科学英文版)
中国海洋大学学报(自然科学英文版)

文圣常(院士)

季刊

1672-5182

xbywb@ouc.edu.cn

0532-82032408

266003

山东省青岛市鱼山路5号

中国海洋大学学报(自然科学英文版)/Journal Journal of Ocean University of ChinaCSCDCSTPCD北大核心SCI
查看更多>>创刊宗旨:我刊及时反映和报道海洋科学与工程、海洋环境科学与工程、海洋气象、海洋药物、海洋遥感、海洋信息系统与管理工程、水产、湖沼等领域的最新研究成果。论文栏目设置:研究论文、技术报告、研究快报、高新技术与信息跟踪、特约评介、综述等。读者对象:国内海洋及相关学科领域的科技人员、教师和管理人员,主要是国际海洋科学界的同行,以英语为语言工具的读者。
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    Effect of Skeleton Grain Size on the Saturation of Gas Hydrate in Natural Sediments

    XU ZhenqiangLI Yang
    676-682页
    查看更多>>摘要:Natural gas hydrate is prospected as a new and promising,highly clean energy resource that mainly occurs in perma-frost or at continental margins.Its formation is subject to many soil conditions,such as grain size,matrix materials,pore morphology,and permeability.In this study,we propose that grain size is the most decisive parameter that affects the saturation of gas hydrate in se-diments based on data from Ocean Drilling Program Leg 164 and Mallik 5L-38,which represent marine sediments and terrestrial sedi-ments,respectively.Our study reveals that high gas hydrate saturation generally occurs in coarse-grained sand,regardless of whether sediment formation is homogeneous or inhomogeneous,and the sorting of sediments may affect the hydrate saturation to a certain degree.Using grain size and sorting of sediments may be the most intuitive proxy method for a rough estimation of hydrate saturation.Further study is necessary to fully understand the relationship between hydrate morphology and sediment grain size,even though massive hydrates are typically found in fine clayey-rich sediments.

    Auto-Detection Method Using Convolution Neural Network for Bottom-Simulating Reflectors

    XU HaoweiXING JunhuiYANG BoxueLIU Chuang...
    683-694页
    查看更多>>摘要:In studies on gas hydrate,bottom-simulating reflectors(BSR)are used to determine the potential hydrate-bearing sedimen-tary layers.Usually,BSR detection is performed manually by experienced interpreters.Therefore,a method for implementing an auto-matic BSR detection process should be established.In this study,we develop a novel architecture for BSR characterization using the convolutional neural network(CNN)technique.We propose the use of Stokes'transform(ST)to obtain a time-frequency spectrum for the input of CNN.ST fully uses the frequency content of the seismic data,and a part of the 3D seismic data collected from the Blake Ridge is utilized to train the CNN.Synthetic seismic records with variable signal-to-noise ratios(SNR),as well as Blake Ridge seismic data,were used to validate the detection effect of the CNN.Results show that the CNN trained by this method exhibits excellent per-formance in noise-resistant testing and achieves an accuracy of more than 89%in field seismic data detection.

    Synergetic Bioproduction of Short-Chain Fatty Acids from Waste Activated Sludge Intensified by the Combined Use of Potassium Ferrate and Biosurfactants

    CHEN YanyanYAO ShuoZHANG DahaiLI Xianguo...
    695-709页
    查看更多>>摘要:The synergetic effect and underlying mechanism of potassium ferrate(PF)with tea saponin(TS,a biosurfactant)in producing short chain fatty acids(SCFAs)from anaerobic fermentation of waste activated sludge(WAS)were explored in this work.Experimental results showed that 0.2gPF(gTSS)-1(total suspended solid)combined with 0.02gTS(gTSS)-1 could further improve SCFAs'production,and the maximum SCFAs content reached 2008.7mgCODL-1,which is 1.2 and 4.5 times higher than those with PF and TS individually added,respectively,and 5.3 times higher than that of blank WAS on Day 12.In the model substrates experi-ments,the degradation rates of bovine serum albumin and dcxtran with combination of PF and TS were 41.3%±0.1%and 48.5%±0.06%,respectively,on Day 3,which are lower than those in blank WAS(with degradation rates of 72.3%±0.5%and 90.3%±0.3%).It was revealed that the oxidative effect of PF and the solubilization of TS caused more organic matters to be dissolved out from WAS,providing a large number of biodegradable substances for subsequent SCFAs production.While WAS pretreated with the com-bination of PF and TS,the relative abundances of Firmicutes increased from 6.4%(blank)to 38.6%,and that of Proteobacteria de-creased from 41.8%(blank)to 21.8%.The combination of PF and TS promoted the hydrolysis process of WAS by enriching Fir-micutes,and then increased acetic acid production by inhibiting Proteobacteria that consumed SCFAs.Meanwhile,at the genus level,acidogenesis bacteria(e.g.,Proteiniclasticum and Petrimonas)were enriched whereas SCFAs consuming bacteria(e.g.,Dokdonella)were inhibited.

    Classification of Sailboat Tell Tail Based on Deep Learning

    CHANG XiaofengYU JintaoGAO YingDING Hongchen...
    710-720页
    查看更多>>摘要:The tell tail is usually placed on the triangular sail to display the running state of the air flow on the sail surface.It is of great significance to make accurate judgement on the drift of the tell tail of the sailboat during sailing for the best sailing effect.Nor-mally it is difficult for sailors to keep an eye for a long time on the tell sail for accurate judging its changes,affected by strong sunlight and visual fatigue.In this case,we adopt computer vision technology in hope of helping the sailors judge the changes of the tell tail in ease with ease.This paper proposes for the first time a method to classify sailboat tell tails based on deep learning and an expert guidance system,supported by a sailboat tell tail classification data set on the expert guidance system of interpreting the tell tails states in different sea wind conditions,including the feature extraction performance.Considering the expression capabilities that vary with the computational features in different visual tasks,the paper focuses on five tell tail computing features,which are re-coded by an automatic encoder and classified by a SVM classifier.All experimental samples were randomly divided into five groups,and four groups were selected from each group as the training set to train the classifier.The remaining one group was used as the test set for testing.The highest resolution value of the ResNet network was 80.26%.To achieve better operational results on the basis of deep computing features obtained through the ResNet network in the experiments.The method can be used to assist the sailors in making better judgement about the tell tail changes during sailing.

    Correlation Study on Grain Size Characteristics and Geotechnical Properties of Surface Sediments in Qingdao Offshore Area

    LI AnlongWANG PanpanGUO XijunJI Xiangkun...
    721-730页
    查看更多>>摘要:The comprehension of sediment grain size parameters and the corresponding sedimentary environment holds paramount importance in elucidating the engineering geological attributes of the subaqueous seabed.This study delineated the sedimentary en-vironment zoning in the northern sea area of Qingdao through cluster analysis of grain size parameters derived from 123 surface sediment samples.The study analyzed the correlation between sediment geotechnical indices and grain size parameters across di-verse sedimentary environments.A correlation equation was established for samples exhibiting a strong correlation.The study found four distinct sedimentary environments in the study area:coastal,transitional,shallow sea,and residual.Within the same sedimentary environment,the average grain size and sorting coefficient exhibit significant correlations with geotechnical indices such as water content,density,shear strength,plastic limit,liquid limit,and plastic index.However,notable disparities in the correlation between grain size parameters and geotechnical indices emerge across different sedimentary environments.

    Autotomy and Regeneration of Appendages in Crustaceans:A Review

    LIU LeiTAO DandanWANG ChunlinFU Yuanyuan...
    731-742页
    查看更多>>摘要:Autotomy of appendages is a self-protection mechanism in crustaceans,which is defined as the reflexive loss of a limb in response to predation,competition,or other environmental factors.Single-limb injuries were the most common among the species surveyed in the present study,and the chelicerae were the most frequently lost appendages.After autotomy,hormones and signaling pathways are altered.Loss of limbs can affect foraging efficiency,although cheliped loss may be compensated by shifting to altema-tive prey or using both motor and oral appendages.In heterogeneous species,the loss of the major chelae may affect the selectivity of feeding.Autotomy can affect crustacean growth by reducing size increases at molting and altering the timing of ecdysis.In commer-cial production,removing chelicerae is an effective strategy to reduce cannibalism,and production of soft-shell crabs can be increased via autotomy.After autotomy,a new limb will regrow through regeneration and molting.This process involves the regulation of hor-mones,regrowth of nerves,and a number of signaling pathways that include the Wnt/β-catenin signaling pathway and transforming growth factor β signaling pathway.Crustaceans are somewhat different from vertebrates in terms of regeneration.This review provides theoretical guidance about autotomy and regeneration applied in artificial aquaculture,and we offer several suggestions for future re-search on autotomy and regeneration in crustaceans.

    Tilapia Head Glycolipid Alleviates Indomethacin-Induced Gastric Ulcer via Regulating Oxidative Stress and Inflammation Through COX/PGE2 Signaling Pathway in Adult Rats

    WANG BohuiWU HaixingSHAO MingyangJIANG Mengqi...
    743-754页
    查看更多>>摘要:The aim of this experiment was to investigate the ameliorative effect and molecular mechanism of tilapia head glycoli-pid(TH-GL)on indomcthacin(IDM)-induced gastric ulcer in male Sprague Dawley(SD)rats.The gastric ulcer model was establish-ed by oral administration of 30 mg kg-1 IDM after 7 days of TH-GL or omeprazole(OME)administration in rats.Then the macrosco-pic gastric injury symptoms,gastric mucosa protective factor cyclooxygenase 1(COX-1),cyclooxygenase 2(COX-2),prostaglandin E2(PGE2),the levels of oxidative stress,and inflammatory cytokine expression levels in the rats were analyzed.The experimental re-sults showed that multiple ulcers appeared on the gastric surface of the rats in the model group.Compared to the model group,TH-GL significantly alleviated gastric ulcers and reduced the gastric damage index in rats.In addition,TH-GL significantly promoted the ex-pression of constitutive enzyme COX-1 while inhibited the expression of inducible enzyme COX-2,and make PGE2 maintain at nor-mal levels.TH-GL also inhibited oxidative stress and inflammatory responses,increased superoxide dismutase(SOD)activity and glu-tathione(GSH)content,decreased the level of malondialdehyde(MDA)and the content of pro-inflammatory factor.In conclusion,these results suggested that TH-GL could maintain the expression levels of COX-1 and PGE2 while inhibit the expression of COX-2 in the gastric of rat and then prevent IDM-induced gastric ulcer,which may be related to the regulation of oxidative stress and inflammatory response.Therefore,TH-GL might be a new option for the prevention of gastric diseases induced by IDM.

    Brain Transcriptome Analysis Reveals Metabolic Changes Adapting to Hyperhaline or Hypohaline Environments in Spotted Scat(Scatophagus argus)

    LIN TingZHAI YiTIAN ChangxuJIANG Dongneng...
    755-765页
    查看更多>>摘要:The fish brain is crucial for adjusting to environmental changes.Metabolic changes play a vital role in the adaptation to salinity change in aquatic animals.However,few studies have evaluated the responses of the fish brain to salinity changes.To evalu-ate the response to various salinities,spotted scat(Scatophagus argus)was cultured in water with salinity levels of 5(low salinity:LS),25(control group:Ctrl),and 35(high salinity group:HS)for 22 days.The brain transcriptome was analyzed.In total,1698 diffe-rentially expressed genes(DEGs)were identified between the HS and Ctrl groups,and 841 DEGs were identified between the LS and Ctrl groups.KEGG analysis showed that the DEGs in the HS vs.Ctrl comparison were involved in steroid biosynthesis,terpenoid backbone biosynthesis,fatty acid biosynthesis,ascorbate and aldarate metabolism,other types of O-glycan biosynthesis,and fatty acid metabolism.Glyoxylate and dicarboxylate metabolism,one carbon pool by folate,steroid biosynthesis,and cysteine and methionine me-tabolism were significantly enriched in the LS vs.Ctrl comparison.Additionally,the genes related to metabolism(acc,fas,hmgcr,hmgcs1,mvd,soat1,nsdhl,sqle,cel,fdft1,dnmt3a and mtr)were significantly up-regulated in the HS vs.Ctrl comparison.The genes related to metabolism(lipa,sqle,acc,fas,bhmt,mpst,dnmt3a,mtr,hao2,LOC111225351 and hmgcs1)were significantly up-regulated,while hmgcr and soat1 were significantly down-regulated in the LS vs.Ctrl compparison.These results suggest that salinity stress af-fects signaling pathways and genes'expressions involved in metabolic processes in the brain,and the differences in metabolism play an important role in adaptation to hyperhaline or hypohaline environments in spotted scat.This research provides a comprehensive over-view of transcriptional changes in the brain under hyperhaline or hypohaline conditions,which is helpful to understand the mechanisms underlying salinity adaptation in euryhaline fishes.

    Endogenous Trypsin Triggers Free Fluoride Release from Antarctic Krill(Euphausia superba)Cuticle

    PENG YuanhuaiJI WeiJI HongwuHAN Zhiping...
    766-774页
    查看更多>>摘要:During postmortem storage,fluoride in Antarctic krill can be enriched in the muscle.Trypsin,as the most important di-gestive enzyme in Antarctic krill with a high activity in low temperature,plays a potential role in this process.In this study,endo-genous trypsin was purified and its properties were investigated.The involvement of trypsin in the generation of free fluoride from Antarctic krill cuticle was explored.Cuticle microstructure before and after hydrolysis was compared with scanning electron micro-scopy,and the ash samples of the hydrolyzed Antarctic krill cuticle were analyzed with X-ray diffraction,Fourier transform infrared spectroscopy,and electron dispersive spectroscopy,respectively.Mass spectrometry analysis and inhibition tests confirmed that the purified enzyme was endogenous trypsin.Results of the present study indicated that trypsin digestion caused the increases of the concentrations of both fluoride ions and free amino N simultaneously,while the protein coated on the cuticle surface was dissolved too.However,no compositional change was detected in thecuticle inorganic salts.These findings suggest that trypsin triggered free fluoride release from Antarctic krill cuticle.In addition,the kinetics of free fluoride release could be described by the equation CW=(1-0.97-0.006t-0.03e0.0558t)×337.53+10.50.The present study improved the understanding of the role of trypsin in free fluo-ride release from Antarctic krill cuticle,facilitating future studies aimed at reducing the fluoride content in krill protein during An-tarctic krill processing.

    Analysis and Characterization of the GABA Transaminase and Succinate Semialdehyde Dehydrogenase Genes in the Microalga Isochrysis zhanjiangensis in Response to Abiotic Stresses

    TIAN JiaojiaoZHANG LinLU XinyueYE Lingzhi...
    775-785页
    查看更多>>摘要:Gamma-aminobutyric acid(GABA),widely existing in different organisms,is rapidly accumulated in plants in response to environmental stresses.The main biosynthesis and degradation pathways of GABA constitute the GABA shunt,which is tied to the tricarboxylic acid(TCA)cycle.GABA transaminase(GABA-T)and succinate semialdehyde dehydrogenase(SSADH)are two essential enzymes for the GABA degradation pathway.While there are ábundant studies on GABA shunt in higher plants at the phy-siological and genetic levels,research on its role in microalgae remains limited.This study aimed at exploring the function of GABA-T and SSADH genes in Isochrysis zhanjiangensis,an important diet microalga,under different stresses.We cloned two GABA-T ge-nes,IzGABA-T1 and IzGABA-T2,and one SSADH gene IzSSADH from Isochrysis zhanjiangensis and conducted heterologous expres-sion experiments.The results showed that the overexpression of IzGABA-T1 or IzGABA-T2 enhanced the survival rates of yeast tran-sformants under heat or NaCl stress,while the overexpression of IzSSADH improved yeast tolerance to NaCl stress but had no obvi-ous effect on heat stress.Additionally,the results of quantitative real-time polymerase chain reaction(qPCR)showed that IzGABA-T1 transcription increased in the HT(salinity 25,35℃)and LS(salinity 15,25℃)groups.At 24 h,the IzGABA-T2 transcriptions in-creased in the HT,LS,and HS(salinity 35,25℃)groups,but their transcription levels decreased in all groups at 48 h.IzSSADH tran-scription increased in the LS group.These results suggest that IzGABA-T1,IzGABA-T2,and IzSSADH are associated with tempera-ture and salinity stresses and possess a certain preference for different stresses.