期刊,中医科学杂志（英文） 2024年卷4期_国家学术搜索

期刊信息/Journal information

中医科学杂志（英文）

北京中医药大学、清华大学出版社有限公司

主办单位：北京中医药大学、清华大学出版社有限公司

出版周期：季刊

国际刊号：2095-7548

电子邮箱：bucmjtcms@163.ccom

电　　话：010-64286200

邮政编码：100029

地　　址：北京市朝阳区北三环东路11号

中医科学杂志（英文）/Journal Journal of Traditional Chinese Medical Sciences

查看更多>>《中医科学杂志（英文）》是中医药领域的同行评审杂志，创刊于2014年7月，由教育部主管，北京中医药大学和清华大学出版社联合主办，国际出版集团Elsevier负责在线出版和开放获取,北京中医药大学校长徐安龙教授担任主编。本杂志致力于搭建最具权威性的英文平台，向国际输出最前沿、最严谨的中医药研究成果，传播各国中医药政策及相关产业发展的信息，反映当今世界中医药研究最高水准。杂志主要收录原创研究类文章，内容覆盖中医、中药、针灸领域，包括各种基础和临床研究，以及综述、政策新闻和病例报告。

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Xuebijing enhances antitumor efficacy of anti-CD19 CAR-T cells

Jingjing ZhuJing ZhangPing WangXiuying Liu...

466-475页

查看更多>>摘要：Objective:To investigate the effects and mechanisms of Xuebijing injection(XBJ)on Chimeric antigen receptor-T(CAR-T)cell function and its therapeutic potential against CAR-T therapy-associated cytokine storms(CRS).Methods:Anti-CD19 CAR-Tcells were established based on FMC63 antibodies.Different doses of XBJ(1 and 10 mg/mL)were added to the culture system.Untreated anti-CD19 CAR-T cells served as negative controls.After 48-h co-culture,the effects of XBJ on CAR-Tcell function were assessed.Carboxyfluorescein diacetate succinimidyl ester staining was used to assess the effect of XBJ on CAR-Tcell proliferation.Flow cytometry,luciferase reporter gene assays,and real time cellular analysis were employed to evaluate the effects of XBJ on CAR-T cell cytotoxicity in vitro.RNA-sequencing was performed to analyze the effects of XBJ on CAR-T cell gene expression.Network pharmacology predicted potential XBJ therapeutic targets for CRS,which were verified in a THP-1 macrophage inflammation model.Results:XBJ enhanced both the proliferation and tumor killing capacities of CAR-T cells.Transcriptome analysis showed that XBJ treatment affects multiple genes and pathways in CAR-T cells,with differential gene enrichment in multiple cell proliferation and growth factor pathways.Potential targets for CRS control by XBJ were predicted using network pharmacology,and the inhibitory effect of XBJ on the expression of relevant genes was verified using a macrophage model.Conclusion:The results of this study indicate that XBJ can enhance the killing effect of CAR-T cells on tumor cells and that the mechanism is related to the regulation of T cell proliferation and activation.Moreover,XBJ inhibited excessive inflammation associated with CAR-T therapy.However,the current findings remain to be further validated through in vivo experiments.

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Antiparasitic and apoptotic modulatory activities of curcumin and extracts of Nigella sativa L,Zingiber officinale Rosc.,and Punica granatum L.in combination with spiramycin against chronic cerebral toxoplasmosis in immunocompromised mice

Mennat-Elrahman A.FahmyAmany A.Abdel-AalSoad I.HassanMaisa A.Shalaby...

476-487页

查看更多>>摘要：Objective:To examine the effect of coadministration of different medicinal plant extracts(curcumin and extracts of Nigella sativa L.[N.sativa,Jia Hei Zhong Cao],Zingiber officinale Rosc.[Z officinale,Sheng Jiang],and Punica granatum L[P.granatum,Shi Liu])with spiramycin(SP)against the cystogenic ME-49 Toxoplasma gondii(T.gondii)strain in immunocompromised mice.Methods:We utilized 68 mice categorized into 8 groups:2 non-infected controls(immunocompetent and immunocompromised),1 infected control,and 5 infected and treated groups.Following the experiment,the cerebral tissues of each mouse underwent parasitological,histopathological,and immunohistochemical evaluations.Results:Compared with the infected non-treated group,all infected treated groups showed significant reductions in brain cyst numbers(all P＜.05),with the highest reduction rate(77.4％)recorded for P.granatum and SP combination(G8).Only G8 showed a significant reduction in mouse deaths compared with the other groups.With regards to restoring histopathological changes and decreasing inflammation,the groups infected and treated with curcumin and P.granatum combined with SP showed the best results(P＜.05).Combinations of curcumin and extracts of Z officinale and P.granatum with SP signif-icantly restored the cerebral expression of caspase-3 compared with the N.sativa extract combination(P＜.05).G8 showed non-significant expression compared with the local expression in the negative control groups.Conclusion:Our study revealed that the coadministration of P.granatum extract with SP was the most effective combination against chronic cerebral toxoplasmosis.

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Energy metabolism,immune function,and intestinal flora in rats with kidney-yin deficiency treated with raw or saltwater-processed Phellodendron chinense Schneid

Wenjing RenJiahui ZhaoLi LiXiutong Ge...

488-499页

查看更多>>摘要：Objective:To clarify the specific mechanisms of action of raw Phellodendron chinense Schneid.(RPC)and saltwater-processed PC(SPC)in the treatment of rats with a kidney-yin deficiency pattern(KYDP).Methods:Healthy rats were administered hydrocortisone to establish a KYDP model.The rats were divided into seven groups:blank control,model,positive control(Liuwei Dihuang pills),high-dose RPC,low-dose RPC,high-dose SPC,and low-dose SPC.Enzyme-linked immunosorbent assay was used to measure the levels of cAMP,cGMP,TRH,TSH,T3,T4,IFN-γ,TNF-α,and testosterone in the serum and the levels of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in the liver.TRH mRNA expression in the rat hypo-thalamus was measured using RT-PCR.THRα1+2 protein expression in the hypothalamus of rats was measured using Western blot.Immunohistochemistry was performed to determine the expression levels of FAS,FasL,and TSHR.Flow cytometry was used to determine CD4+and CD8+T lymphocyte levels.Illumina MiSeq sequencing technology was used to evaluate the diversity of intestinal flora in KYDP rats.Results:The cAMP/cGMP ratio was significantly higher in the model group than in the blank control group(P=.048).Compared with the model group,after administration,the levels of the above-mentioned serum and liver indexes decreased,except that of testosterone.The CD4+/CD8+ratio also decreased.Compared with the RPC group,the levels of T3,1FN-γ,FAS,FasL,and TSHR in the SPC group decreased whereas that of testosterone increased.Additionally,immune function and intestinal flora diversity improved in the SPC group.SPC proved to be more effective in improving liver energy meta-bolism in KYDP rats than RPC.Conclusion:SPC had a better therapeutic effect on KYDP than RPC.The underlying mechanism of action may be related to improvements in liver energy metabolism,immune function,and intestinal flora diversity.

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Effects of acupuncture on serotonin,histamine,substance P,and tryptase levels at sensitized points in model rats with knee osteoarthritis

Jiayi YangZidong WangJing JiangHuiling Tian...

500-512页

查看更多>>摘要：Objective:To elucidate the differences in manual acupuncture effectiveness at sensitized points by investigating the mechanisms of local skin action at different sensitization points in rats with knee osteoarthritis(KOA).Methods:Forty Sprague-Dawley rats were equally divided into control,model(1 mg of mono-iodoacetate into the right knee joint cavity),sham operation,manual acupuncture at right Tianjing acupoint(MAR-SJ 10),and left SJ 10 groups.Safranine-O and fast green staining were used to assess the modeling.The morphological and functional changes in mast cells(MCs)were assessed during acupoint sensitization using toluidine blue and immunofluorescence staining.The levels of serotonin,histamine,substance P(SP),and tryptase at skin acupoints and serum levels of IL-β,IL-6,and TNF-α were detected using ELISA.Results:After 14 days of treatment,the number of MCs and their degranulation rates were statistically higher in the model group than in the control group(both P＜.001).After applying acupuncture,the levels of 5-HT,HA,and SP at skin acupoints were lower than those in the model group(all P＜.05),and tryptase level was higher(both P＜.05).Tryptase level was higher on the skin at the MAL-SJ10 acupoint than that on the MAR-SJ 10 acupoint(P=.004).Compared with the model group,the serum levels of IL-1β,IL-6,and TNF-α in the MAR-SJ 10 and MAL-SJ 10 groups were lower(all P＜.05).Conclusion:Acupuncture at KOA-sensitized acupoints mitigates joint injury in KOA rats and may bidi-rectionally regulate local MCs of these acupoints.This finding not only enhances the reference value of sensitizing points in clinical diagnosis and treatment,but also contributes to the understanding of the biological mechanisms underlying acupuncture intervention at sensitizing points.

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The role of brevican regulation in the antidepressant effects of electroacupuncture in a chronic stress rat model

Cong GaiZhenyu GuoKai GuoShixin Yang...

513-521页

查看更多>>摘要：Objective:To investigate the mechanism of electroacupuncture(EA)for treating depression and to explore the role of brevican in the medial prefrontal cortex(mPFC)in modulating stress susceptibility and the antidepressant effects of EA in rats.Methods:Twenty-four Sprague-Dawley(SD)rats were equally divided into three groups:green fluo-rescent protein(GFP)+control,GFP+chronic unpredicted mild stress(CUMS),and short-hairpin RNA targeting on brevican(shBcan)+CUMS.Another 24 SD rats were equally divided into CUMS+GFP,CUMS+GFP+EA,and CUMS+shBcan+EA groups.Behavioral tests were conducted to assess depression-like behavior.Western blot analysis was used to evaluate the expression of brevican,aggrecan,GLuA1,and PSD95 in mPFC subregions.Results:Behavioral parameter evaluation show that rats in the shBcan+CUMS group exhibited a significantly reduced sucrose preference(P=.0002)and increased immobility time(P=.0011)compared to those in rats in the GFP+CUMS group.Western blotting showed that brevican expression was significantly downregulated in the PrL of the shBcan+CUMS group compared with that in the GFP+CUMS group(P=.0192).Furthermore,compared to the CUMS+GFP+EA group,the CUMS+shBcan+EA group exhibited a significantly decreased sucrose preference(P=.0334),increased immobility time(P=.0465),and increased latency to food(P=.0261).In the CUMS+shBcan+EA group,the EA-induced brevican and PSD95 overexpression was reversed,compared with that in the CUMS+GFP+EA group(P=.0454 and P=.0198,respectively).Conclusion:EA exerts its antidepressant effects through the modulation of brevican expression in rats.Our findings highlight the important role for brevican in stress susceptibility,which could be a potential target for treating depression.

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