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畜牧与生物技术杂志(英文版)
中国科学技术协会
畜牧与生物技术杂志(英文版)

中国科学技术协会

李德发

季刊

1674-9782

zgxm@cau.edu.cn;xmzz@cau.edu.cn

010-62734403/2723

100193

中国农业大学西校区动物科技学院Journal of Animal Science and Biotechnology编辑部

畜牧与生物技术杂志(英文版)/Journal Journal of Animal Science and BiotechnologyCSCDCSTPCD北大核心SCI
查看更多>>定位为国内外公开发行的专业学术期刊，宗旨是立足国内，面向国际，为国内外畜牧科技工作者提供国际化学术共享的园地，促进我国与国际上其他国家在畜牧科学领域的交流；让中国畜牧科技走向世界，让世界了解中国畜牧科技。
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    Astragalus polysaccharides-induced gut microbiota play a predominant role in enhancing of intestinal barrier function of broiler chickens

    Jiantao YangYanpeng SunQianggang WangShanglin Yu...
    2524-2542页
    查看更多>>摘要：Background The intestinal barrier is the first line of defense against intestinal invasion by pathogens and foreign antigens and is closely associated with the gut microbiota.Astragalus polysaccharides(APS)have a long history of use in traditional Chinese medicine owing to its protective properties against intestinal barrier function.The mechanism of APS-induced gut microbiota enhancing intestinal barrier function is urgently needed.Results Dietary polysaccharide deprivation induced intestinal barrier dysfunction,decreased growth performance,altered microbial composition(Faecalibacterium,Dorea,and Coprobacillus),and reduced isobutyrate concentration.The results showed that APS facilitates intestinal barrier function in broiler chickens,including a thicker mucus layer,reduced crypt depth,and the growth of tight junction proteins.We studied the landscape of APS-induced gut micro-biota and found that APS selectively promoted the growth of Parabacteroides,a commensal bacterium that plays a predominant role in enhancing intestinal barrier function.An in vitro growth assay further verified that APS selec-tively increased the abundance of Parabacteroides distasonis and Bacteroides uniformis.Dietary APS supplementation increased the concentrations of isobutyrate and bile acid(mainly chenodeoxycholic acid and deoxycholate acid)and activated signaling pathways related to intestinal barrier function(such as protein processing in the endoplasmic reticulum,tight junctions,and adherens junction signaling pathways).Conclusions APS intervention restored the dietary polysaccharide-induced dysfunction of the intestinal barrier by selectively promoting the abundance of Parabacteroides distasonis,and increasing the concentrations of isobu-tyrate and bile acids(mainly CDCA and DCA).These findings suggest that APS-induced gut microbiota and metabolic niches are promising strategies for enhancing intestinal barrier function.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
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    Effects of grain intervention on hypothalamic function and the metabolome of blood and milk in dairy cows

    Limei LinKaizhen GuoHuiting MaJiyou Zhang...
    2543-2552页
    查看更多>>摘要：Background The hypothalamus plays a crucial role in the health and productivity of dairy cows,yet studies on its functionality and its impact on peripheral circulation in these animals are relatively scarce,particularly regard-ing dietary interventions.Therefore,our study undertook a comprehensive analysis,incorporating both metabolomics and transcriptomics,to explore the effects of a grain-based diet on the functionality of the hypothalamus,as well as on blood and milk in dairy cows.Results The hypothalamic metabolome analysis revealed a significant reduction in prostaglandin E2(PGE2)level as a prominent response to the grain-based diet introduction.Furthermore,the hypothalamic transcriptome profiling showed a notable upregulation in amino acid metabolism due to the grain-based diet.Conversely,the grain-based diet led to the downregulation of genes involved in the metabolic pathway from lecithin to PGE2,including phospho-lipase A2(PLA2G4E,PLA2G2A,and PLA2G12B),cyclooxygenase-2(COX2),and prostaglandin E synthase(PTGES).Addi-tionally,the plasma metabolome analysis indicated a substantial decrease in the level of PGE2,along with a decline in adrenal steroid hormones(tetrahydrocortisol and pregnenolone)following the grain-based diet introduction.Analysis of the milk metabolome showed that the grain-based diet significantly increased uric acid level while notably decreasing PGE2 level.Importantly,PGE2 was identified as a critical metabolic marker in the hypothalamus,blood,and milk in response to grain intervention.Correlation analysis demonstrated a significant correlation among meta-bolic alterations in the hypothalamus,blood,and milk following the grain-based diet.Conclusions Our findings suggest a potential link between hypothalamic changes and alterations in peripheral circulation resulting from the introduction of a grain-based diet.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Dietary silymarin improves performance by altering hepatic lipid metabolism and cecal microbiota function and its metabolites in late laying hens

    Yanghao GuoYudong XuDerun WangShihao Yang...
    2553-2570页
    查看更多>>摘要：Background Liver lipid dysregulation is one of the major factors in the decline of production performance in late-stage laying hens.Silymarin(SIL),a natural flavonolignan extracted from milk thistle,is known for its hepatoprotective and lipid-lowering properties in humans.This study evaluates whether SIL can provide similar benefits to late-stage laying hens.A total of 480 68-week-old Lohmann Pink laying hens were randomly assigned into 5 groups,each group consisting of 6 replicates with 16 hens each.The birds received a basal diet either without silymarin(control)or supplemented with silymarin at concentrations of 250,500,750,or 1,000 mg/kg(SIL250,SIL500,SIL750,SIL1000)over a 12-week period.Results The CON group exhibited a significant decline in laying rates from weeks 9 to 12 compared to the initial 4 weeks(P=0.042),while SIL supplementation maintained consistent laying rates throughout the study(P＞0.05).Notably,the SIL500 and SIL750 groups showed higher average egg weight than the CON group during weeks 5 to 8(P=0.049).The SIL750 group had a significantly higher average daily feed intake across the study period(P＜0.05),and the SIL500 group saw a marked decrease in the feed-to-egg ratio from weeks 5 to 8(P=0.003).Furthermore,the SIL500 group demonstrated significant reductions in serum ALT and AST levels(P＜0.05)and a significant decrease in serum triglycerides and total cholesterol at week 12 with increasing doses of SIL(P＜0.05).SIL also posi-tively influenced liver enzyme expression(FASN,ACC,Apo-VLDL Ⅱ,FXR,and CYP7A1;P＜0.05)and altered the cecal microbiota composition,enhancing species linked to secondary bile acid synthesis.Targeted metabolomics identi-fied 9 metabolites predominantly involved in thiamin metabolism that were significantly different in the SIL groups(P＜0.05).Conclusions Our study demonstrated that dietary SIL supplementation could ameliorate egg production rate in late stage laying hens,mechanistically,this effect was via improving hepatic lipid metabolism and cecal microbiota function to achieve.Revealed the potentially of SIL as a feed supplementation to regulate hepatic lipid metabolism dysregulation.Overall,dietary 500 mg/kg SIL had the best effects.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
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    Nano-Se exhibits limited protective effect against heat stress induced poor breast muscle meat quality of broilers compared with other selenium sources

    Jinzhong JingJiayi WangQian WuShenggang Yin...
    2571-2588页
    查看更多>>摘要：Background At present,heat stress(HS)has become a key factor that impairs broiler breeding industry,which causes growth restriction and poor meat quality of broilers.Selenium(Se)is an excellent antioxidant and plays a unique role in meat quality improvement.Recent years,nano-selenium(NanoSe)has received tremendous atten-tion in livestock production,due to its characteristic and good antibacterial performance in vitro.Here,we developed the heat stressed-broiler model to investigate the protective effects of NanoSe on growth performance and meat quality of broilers and compare whether there are differences with that of other Se sources(Sodium selenite,SS;Sele-noyeast,SeY;Selenomethionine,SeMet).Results HS jeopardized the growth performance and caused poor meat quality of breast muscle in broilers,which were accompanied by lowered antioxidant capacity,increased glycolysis,increased anaerobic metabolism of pyruvate,mitochondrial stress and abnormal mitochondrial tricarboxylic acid(TCA)cycle.All Se sources supple-mentation exhibited protective effects,which increased the Se concentration and promoted the expression of sele-noproteins,improved the mitochondrial homeostasis and the antioxidant capacity,and promoted theTCA cycle and the aerobic metabolism of pyruvate,thus improved the breast muscle meat quality of broilers exposed to HS.However,unlike the other three Se sources,the protective effect of NanoSe on meat quality of heat stressed-broilers was not ideal,which exhibited limited impact on the pH value,drip loss and cooking loss of the breast muscle.Compared with the other Se sources,broilers received NanoSe showed the lowest levels of slow MyHC,the highest levels of fast MyHC and glycogen,the highest mRNA levels of glycolysis-related genes(PFKM and PKM),the highest protein expression of HSP60 and CLPP,and the lowest enzyme activities of GSH-Px,citroyl synthetase(CS)and isoci-trate dehydrogenase(ICD)in breast muscle.Consistent with the SS,the Se deposition in breast muscle of broilers received NanoSe was lower than that of broilers received SeY or SeMet.Besides,the regulatory efficiency of NanoSe on the expression of key selenoproteins(such as SELENOS)in breast muscle of heat stressed-broilers was also worse than that of other Se sources.Conclusion Through comparing the meat quality,Se deposition,muscle fiber type conversion,glycolysis,mitochon-drial homeostasis,and mitochondrial TCA cycle-related indicators of breast muscle in heat stressed broilers,we found that the protective effects of organic Se(SeY and SeMet)are better than that of inorganic Se(SS)and NanoSe.As a new Se source,though NanoSe showed some protective effect on breast muscle meat quality of heat stressed broil-ers,the protective effect of NanoSe is not ideal,compared with other Se sources.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
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    Hepatoprotective effects of magnolol in fatty liver hemorrhagic syndrome hens through shaping gut microbiota and tryptophan metabolic profile

    Yujie LvChaoyue GeLianchi WuZhaoying Hu...
    2589-2606页
    查看更多>>摘要：Background Magnolol(MAG)exhibits hepatoprotective activity,however,whether and how MAG regulates the gut microbiota to alleviate fatty liver hemorrhagic syndrome(FLHS)remains unclear.Therefore,we investigated the mech-anism of MAG in FLHS laying hens with an emphasis on alterations in the gut-liver axis.We randomly divided 54056-week-old Hy-line white laying hens with FLSH into 4 groups.The birds were fed a high-fat low-protein(HFLP)diet(CON)or HELP diets supplemented with 200,400,and 600 mg/kg of MAG(M1,M2,and M3,respectively)for 9 weeks.Results Magnolol supplementation increased the laying rate and ameliorated hepatic damage and dysfunction by regulating lipid metabolism,improving intestinal barrier function,and shaping the gut microbiota and tryptophan metabolic profiles.Dietary MAG supplementation downregulated the expression of lipid synthesis genes and upregu-lated the expression of lipid transport genes at varying degrees.The intestinal barrier function was improved by 200 and 400 mg/kg of MAG supplementation,as evidenced by the increased villus height and mRNA expression of tight junction related genes.Microbiological profile information revealed that MAG changed the gut microbiota,espe-cially by elevating the abundances of Lactobacillus,Faecalibacterium,and Butyricicoccus.Moreover,non-targeted metabolomic analysis showed that MAG significantly promoted tryptophan metabolites,which was positively correlated with the MAG-enriched gut microbiota.The increased tryptophan metabolites could activate aryl hydro-carbon receptor(AhR)and relieved hepatic inflammation and immune response evidenced by the downregulated the gene expression levels of pro-inflammatory cytokines such as interleukin-1(3(IL-1β),tumor necrosis factor-a(TNF-a),and interleukin-6(IL-6)in the liver.The fecal microbiota transplantation(FMT)experiments further confirmed that the hepatoprotective effect is likely mediated by MAG-altered gut microbiota and their metabolites.Conclusions Magnolol can be an outstanding supplement for the prevention and mitigation of FLHS in laying hens by positively regulating lipid synthesis and transport metabolism,improving the intestinal barrier function,and reliev-ing hepatic inflammation by reshaping the gut microbiota and metabolite profiles through gut microbiota-indole metabolite-hepatic AhR crosstalk.These findings elucidate the mechanisms by which MAG alleviates FLHS and pro-vide a promising method for preventing liver diseases by modulating gut microbiota and their metabolites.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
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    Methionine deficiency inhibited pyroptosis in primary hepatocytes of grass carp(Ctenopharyngodon idella):possibly via activating the ROS-AMPK-autophagy axis

    Yuanlin HePei WuWeidan JiangYang Liu...
    2607-2624页
    查看更多>>摘要：Background Methionine(Met)is the only sulfur-containing amino acid among animal essential amino acids,and methionine deficiency(MD)causes tissue damage and cell death in animals.The common modes of cell death include apoptosis,autophagy,pyroptosis,necroptosis.However,the studies about the major modes of cell death caused by MD have not been reported,which worth further study.Methods Primary hepatocytes from grass carp were isolated and treated with different doses of Met(0,0.5,1,1.5,2,2.5 mmol/L)to examine the expression of apoptosis,pyroptosis,autophagy and necroptosis-related proteins.Based on this,we subsequently modeled pyroptosis using lipopolysaccharides and nigericin sodium salt,then autophagy inhibitors chloroquine(CQ),AMP-activated protein kinase(AMPK)inhibitors compound C(CC)and reactive oxygen species(ROS)scavengers N-acetyl-L-cysteine(NAC)were further used to examine the expression of proteins related to pyroptosis,autophagy and AMPK pathway in MD-treated cells respectively.Results MD up-regulated B-cell lymphoma protein 2(Bax),microtubule-associated protein 1 light chain 3 Ⅱ(LC3 Ⅱ),and down-regulated the protein expression levels of B-cell lymphoma-2(Bcl-2),sequestosome 1(p62),cleaved-caspase-1,cleaved-interleukin(IL)-1 β,and receptor-interacting protein kinase(RIP)1 in hepatocytes,while it did not significantly affect RIP3.In addition,MD significantly increased the protein expression of liver kinase B1(LKB1),p-AMPK,and Unc-51-like kinase 1(ULK1)without significant effect on p-target of rapamycin.Subsequently,the use of CQ increased the protein expression of NOD-like receptor thermal protein domain associated protein 3(NLRP3),cleaved-caspase-1,and cleaved-IL-1β inhibited by MD;the use of CC significantly decreased the protein expression of MD-induced LC3 Ⅱ and increased the protein expression of MD-suppressed p62;then the use of NAC decreased the MD-induced p-AMPK protein expression.Conclusion MD promoted autophagy and apoptosis,but inhibited pyroptosis and necroptosis.MD inhibited pyrop-tosis may be related regarding the promotion of autophagy.MD activated AMPK by inducing ROS production which in turn promoted autophagy.These results could provide partial theoretical basis for the possible mechanisms of Met in ensuring the normal structure and function of animal organs.Furthermore,ferroptosis is closely related to redox states,it is worth investigating whether MD affects ferroptosis in hepatocytes.
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    • 国家科技期刊平台
    • NETL
    • NSTL
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    Effects of bacteriocin-producing Lactiplantibacillus plantarum on bacterial community and fermentation profile of whole-plant corn silage and its in vitro ruminal fermentation,microbiota,and CH4 emissions

    Ziqian LiSamaila UsmanJiayao ZhangYixin Zhang...
    2625-2638页
    查看更多>>摘要：Background Silage is widely used to formulate dairy cattle rations,and the utilization of antibiotics and methane emissions are 2 major problems for a sustainable and environmentally beneficial ruminant production systems.Bacteriocin has received considerable attention because of its potential as an alternative to antibiotics in animal hus-bandry.However,the impact of bacteriocin-producing lactic acid bacteria on the microbiological conversion process of whole-plant corn silage and rumen fermentation remains limited.The purpose of this study was to assess the effect of 2 class Ila bacteriocin-producing strains Lactiplantibacillus plantarum ATCC14917 and CICC24194 on bacterial com-munity composition and ensiling profiles of whole-plant corn silage and its in vitro rumen fermentation,microbiota,and CH4 emissions.Results Both bacteriocin-producing strains increased the lactic acid concentration in silage fermented for 7 d,whereas the lowest lactic acid was observed in the ATCC14917 inoculated silage fermented for 90 d(P＜0.05).The highest DM content was observed in the CICC24194 treatment(P＜0.05),and the silages treated with both strains had the lowest DM loss(P＜0.05).Bacteriocin-producing strains promoted the growth of Levilactobacillus brevis on d 60 of ensiling.In addition,treatment with bacteriocin-producing strains increased the in vitro DM digestibility(P＜0.05)and decreased the CH4 production(P＜0.05).The results of random forest and clustering analyses at the genus level showed that ATCC14917 increased the relative abundance of the influential variable Bacillus compared to that in the control group,whereas CICC24194 decreased the relative abundance of the influential variable Ruminococcaceae UCG-005.The CICC24194 treatment had the lowest total bacterial,fungal,protozoan,and methanogen populations(P＜0.05).Conclusions Both class Ⅱa bacteriocin-producing L.plantarum strains improved the fermentation quality of whole-plant corn silage by regulating the bacterial community composition during ensiling,with CICC24194 being the most effective.Both bacteriocin-producing strains mitigated CH4 production and improved digestibility by modulating the interactions among rumen bacteria,protozoa,methanogens,and the composition of fibrolytic bacteria.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Effects of microbe-derived antioxidants on growth performance,hepatic oxidative stress,mitochondrial function and cell apoptosis in weaning piglets

    Chengbing YuYuxiao LuoCheng ShenZhen Luo...
    2639-2655页
    查看更多>>摘要：Background Weaning causes redox dyshomeostasis in piglets,which leads to hepatic oxidative damage.Microbe-derived antioxidants(MA)have great potential for anti-oxidation.This study aimed to investigate changes in hepatic redox system,mitochondrial function and apoptosis after weaning,and effects of MA on growth performance and liver health in weaning piglets.Methods This study consisted of 2 experiments.In the both experiments,piglets were weaned at 21 days of age.In Exp.1,at 21(W0),22(W1),25(W4),28(W7),and 35(W14)days of age,6 piglets were slaughtered at each timepoint.In Exp.2,piglets were divided into 2 groups:one received MA gavage(MA)and the other received saline gavage(CON).At 25 days of age,6 piglets from each group were sacrificed.Results In Exp.1,weaning caused growth inhibition and liver developmental retardation from W0 to W4.The mRNA sequencing between W0 and W4 revealed that pathways related to"regulation of apoptotic process"and"reactive oxygen species metabolic process"were enriched.Further study showed that weaning led to higher hepatic con-tent of reactive oxygen species(ROS),H2O2 and O2-.Weaning enhanced mitochondrial fission and suppressed their fusion,activated mitophagy,thus triggering cell apoptosis.In Exp.2,MA improved growth performance of piglets with higher average daily gain(ADG)and average daily feed intake(ADFI).The hepatic ROS,as well as products of oxidative damage malonaldehyde(MDA)and 8-hydroxy-2'-deoxyguanosine(8-OHdG)in the MA group decreased significantly than that of the CON group.The MA elevated mitochondrial membrane potential,increased activity of mitochondrial respiratory chain complexes(MRC)Ⅰ and Ⅳ,enhanced mitochondrial fusion and reduced mitophagy,thus decreasing cell apoptosis.Conclusions The present study showed that MA improved the growth performance of weaning piglets and reversed weaning-induced oxidative damage,mitochondrial dysfunction,and apoptosis.Our results suggested that MA had promising prospects for maintaining liver health in weaning piglets and provided a reference for studies of liver dis-eases in humans.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
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    Effects of replacing soybean meal with enzymolysis-fermentation compound protein feed on growth performance,apparent digestibility of nutrients,carcass traits,and meat quality in growing-finishing pigs

    Yu ChengJun HePing ZhengJie Yu...
    2656-2668页
    查看更多>>摘要：Background Addressing the shortage of high-quality protein resources,this study was conducted to investigate the effects of replacing soybean meal(SBM)with different levels of enzymolysis-fermentation compound protein feed(EFCP)in the diets of growing-finishing pigs,focusing on growth performance,nutrients digestibility,carcass traits,and meat quality.Methods Sixty DLY(Duroc×Landrace×Yorkshire)pigs with an initial body weight of 42.76+2.05 kg were assigned to 5 dietary treatments in a 2x2+1 factorial design.These dietary treatments included a corn-soybean meal diet(CON),untreated compound protein feed(UCP)substitution 50％(U50)and 100％SBM(U100)diets,and EFCP substitution 50％(EF50)and 100％SBM(EF100)diets.Each treatment had 6 pens(replicates)with 2 pigs per pen,and the experiment lasted 58 d,divided into phase Ⅰ(1-28 d)and phase Ⅱ(29-58 d).Following phase I,only the CON,U50,and EF50 groups were continued for phase Ⅱ,each with 5 replicate pens.On d 59,a total of 15 pigs(1 pig/pen,5 pens/treatment)were euthanized.Results During phase I,the EF50 group had a higher average daily gain(ADG)in pigs(P＜0.05)compared to the CON group,whereas the U50 group did not have a significant difference.As the substitution ratio of UCP and EFCP increased in phase I,there was a noticeable reduction in the final body weight and ADG(P＜0.05),along with an increase in the feed-to-gain ratio(F/G)(P＜0.05).In phase Ⅱ,there were no significant differences in growth perfor-mance among the treatment groups,but EF50 increased the apparent digestibility of several nutrients(including dry matter,crude protein,crude fiber,acid detergent fiber,ash,gross energy)compared to U50.The EF50 group also exhibited significantly higher serum levels of neuropeptide Y and ghrelin compared to the CON and U50 groups(P＜0.05).Moreover,the EF50 group had higher carcass weight and carcass length than those in the CON and U50 groups(P＜0.05),with no significant difference in meat quality.Conclusions The study findings suggest that replacing 50％SBM with EFCP during the growing-finishing period can improve the growth performance,nutrient digestibility,and carcass traits of pigs without compromising meat quality This research offers valuable insights into the modification of unconventional plant protein meals and developing alternatives to SBM.
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    • 国家科技期刊平台
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    • NSTL
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