查看更多>>摘要：Objective:Paris polyphylla var.yunnanensis,one of the important medicinal plant resources in Yunnan,China,usually takes 6-8 years to be harvested.Therefore,it is urgent to find a method that can not only shorten its growth years,but also improve its quality.In this study,we examined the effects of a combi-nation treatment of arbuscular mycorrhizal fungi(AMF)and plant growth-promoting endophytes(PGPE)and drought stress on the accumulation of saponins in it.Methods:P.polyphylla var.yunnanensis was infected with a mixture of AMF and PGPE under drought stress.The content of saponins,as well as morphological,physiological,and biochemical indicators,were all measured.The UGTs gene related to saponin synthesis was obtained from transcriptome data by homologous comparison,which were used for RT-PCR and phylogenetic analysis.Results:Regardless of water,AMF treatment could infect the roots of P.polyphylla var.yunnanensis,how-ever double inoculation with AMF and PGPE(AMF+PGPE)would reduce the infection rate of AMF.Plant height,aboveground and underground fresh weight did not differ significantly between the single inoc-ulation AMF and the double inoculation treatment under different water conditions,but the inoculation treatment significantly increased the plant height of P.polyphylla var.yunnanensis compared to the non-inoculation treatment.Single inoculation with AMF considerably increased the net photosynthetic rate,stomatal conductance,and transpiration rate of P.polyphylla var.yunnanensis leaves under various water conditions,but double inoculation with AMF+PGPE greatly increased the intercellular CO2 concentration and chlorophyll fluorescence parameter(Fv/Fm).Under diverse water treatments,single inoculation AMF had the highest proline content,whereas double inoculation AMF+PGPE may greatly improve the amount of abscisic acid(ABA)and indoleacetic acid(IAA)compared to normal water under moderate drought.Double inoculation AMF+PGPE treatment improved the proportion of N,P,and K in the rhizome of P.polyphylla var.yunnanensis under various water conditions.Under moderate drought stress,AMF+PGPE significantly enhanced the contents of P.polyphylla var.yunnanensis saponins Ⅰ,Ⅱ,VⅡ,and total saponins as compared to normal water circumstances.Farnesyl diphosphate synthase(FPPS),Geranyl pyrophosphate synthase(GPPS),Cycloartenol synthase(CAS),and Squalene epoxidase(SE1)were the genes that were significantly up-regulated at the same time.The amount of saponins was favorably linked with the expression of CAS,GPPS,and SE1.Saponin Ⅵ content and glycosyl transferase(UGT)010922 gene expression were found to be substantially associated,as was saponin Ⅱ content and UGT010935 gene expression.Conclusion:Under moderate drought,AMF+PGPE was more conducive to the increase of hormone con-tent,nutrient absorption,and total saponin content in P.polyphylla var.yunnanensis,and AMF+PGPE could up regulate the expression of key genes and UGTs genes in one or more steroidal saponin synthesis pathways to varying degrees,thereby stimulating the synthesis and accumulation of steroidal saponins in the rhizome of P.polyphylla var.yunnanensis.The combination of AMF and PGPE inoculation,as well as adequate soil drought,reduced the buildup of saponins in P.polyphylla var.yunnanensis and increased its quality.
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