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农业科学学报（英文）
中国农业科学院农业信息研究所
农业科学学报（英文）

中国农业科学院农业信息研究所

翟虎渠

月刊

2095-3119

zgnykx@mail.caas.net.cn

010-82106283 82106280

100081

北京中关村南大街12号

农业科学学报（英文）/Journal Journal of Integrative AgricultureCSCDCSTPCD北大核心SCI
查看更多>>本刊创刊于2002年，由中国农业科学院、中国农学会主办，中国农业科学院农业信息研究所承办。刊登农牧业基础科学和应用科学的研究论文，覆盖作物科学、动物科学、农业环境、农业经济与管理等领域。
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    Establishment of a system for screening and identification of novel bactericide targets in the plant pathogenic bacterium Xanthomonas oryzae pv.oryzae using Tn-seq and SPR

    Chaoyue PangLing JinHaoyu ZangDamalk Saint-Claire S.Koklannou...
    1580-1592页
    查看更多>>摘要：Xanthomonas spp.cause severe bacterial diseases.However,effective strategies for prevention and management of these diseases are scarce.Thus,it is necessary to improve the efficiency of control of diseases caused by Xanthomonas.In this study,Xanthomonas oryzae pv.oryzae(Xoo),which causes rice bacterial leaf blight,has been studied as a representative.A transposon insertion library of Xoo,comprising approximately 200,000 individual insertion mutants,was generated.Transposon sequencing data indicated that the mariner C9 transposase mapped at 35.7-36.4％of all potential insertion sites,revealing 491 essential genes required for the growth of Xoo in rich media.The results show that,compared to the functions of essential genes of other bacteria,the functions of some essential genes of Xoo are unknown,25 genes might be dangerous for the Xanthomonas group,and 3 are specific to Xanthomonas.High-priority candidates for developing broad-spectrum,Xanthomonas-specific,and environment-friendly bactericides were identified in this study.In addition,this study revealed the possible targets of dioctyldiethylenetriamine using surface plasmon resonance(SPR)in combination with high performance liquid chromatography-mass spectrometry(HPLC-MS).The study also provided references for the research of some certain bactericides with unknown anti-bacterial mode of action.In conclusion,this study urged a better understanding of Xanthomonas,provided meaningful data for the management of bacterial leaf blight,and disclosed selected targets of a novel bactericide.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
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    Hatching and development of maize cyst nematode Heterodera zeae infecting different plant hosts

    Jiangkuan CuiHaohao RenBo WangFujie Chang...
    1593-1603页
    查看更多>>摘要：The occurrence,distribution,and rapid molecular detection technology of Heterodera zeae Koshy et al.1971,have been reported in China.We explored the biological characteristics of H.zeae sampled in Henan Province,China to understand its interaction with plants.Cysts and second-stage juveniles(J2s)were identified under an optical and scanning electron microscope,internal transcribed spacer(ITS)phylogenetic tree,and sequence characterized amplified region(SCAR)-PCR analyses.The optimum hatching temperatures of H.zeae were 30℃ and 28℃,with cumulative hatching rates of 16.5 and 16.1％,respectively,at 30 days post-hatching(dph).The hatching rate of H.zeae eggs was improved by 20-and 50-time maize soil leachate and root juice,and 10-time root exudates.The hatching rate in 10-time root exudates was the highest(25.9％).The 10-time root exudates of maize and millet produced the highest hatching rate at 30 dph(25.9 and 22.9％,respectively),followed by wheat(19.9％),barley(18.3％),and rice(17.6％).Heterodera zeae developed faster in maize than in other crops.Fourth-stage juveniles(J4s)were detected in maize roots 8 days post-inoculation(dpi)at 28℃ but not in other crops.Combined with hatching tests,the Huang-Huai-Hai summer maize region and the south and central-southwest mountainous maize areas are highly suitable for H.zeae in China.This is the first systematically study of the hatching and infection characteristics on different plant hosts of corn cyst nematode H.zeae in temperate regions.This study laid a theoretical foundation for the rapid spread and high environmental adaptability of corn cyst nematode.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Functional assessment of cadherin as a shared mechanism for cross/dual resistance to Cry1Ac and Cry2Ab in Helicoverpa zea

    Jizhen WeiMin ZhangPin LiZhongyuan Deng...
    1604-1617页
    查看更多>>摘要：Helicoverpa zea is a major target pest of pyramided transgenic crops expressing Cry1,Cry2 and/or Vip3Aa proteins from Bacillus thuringiensis(Bt)in the United States.Laboratory-selected Cry1Ac/Cry2Ab cross resistance and field-evolved practical dual resistance of H.zea to these two toxins have been widely reported.Whether the widespread Cry1 Ac/Cy2Ab dual resistance of H.zea has resulted from the selection of one shared or two independent resistance mechanisms by pyramided Bt crops remains unclear.Cadherin is a well-confirmed receptor of Cry1Ac and a suggested receptor of Cry2Ab in at least three Lepidopteran species.To test whether cadherin may serve as one shared mechanism for the cross and dual resistance of H.zea to Cry1Ac and Cry2Ab,we cloned H.zea cadherin(HzCadherin)cDNA and studied its functional roles in the mode of action of Cry1Ac and Cry2Ab by gain-and loss-of-function analyses.Heterologous expression of HzCadherin in H.zea midgut,H.zea fat body and Sf9 cells made all three of these cell lines more susceptible to activated Cry1Ac but not activated Cry2Ab,whereas silencing HzCadherin of H.zea midgut and fat body cells significantly reduced the susceptibility to Cry1Ac but not Cry2Ab.Likewise,suppressing HzCadherin with siRNA made H.zea larvae resistant to Cry1Ac.These results clearly demonstrate that HzCadherin is not a receptor for Cry2Ab,and thus it is unlikely to serve as one shared mechanism for the cross and dual resistance of H.zea to Cry1Ac and Cry2Ab.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Low-density lipoprotein receptor-related protein 2(LRP2)is required for lipid export in the midgut of the migratory locust,Locusta migratoria

    Yiyan ZhaoWeimin LiuXiaoming ZhaoZhitao Yu...
    1618-1633页
    查看更多>>摘要：Low-density lipoprotein receptor-related protein 2(LRP2)is a multifunctional endocytic receptor expressed in epithelial cells.In mammals,it acts as an endocytic receptor that mediates the cellular uptake of cholesterol-containing apolipoproteins to maintain lipid homeostasis.However,little is known about the role of LRP2 in lipid homeostasis in insects.In the present study,we investigated the function of LRP2 in the migratory locust Locusta migratoria(LmLRP2).The mRNA of LmLRP2 is widely distributed in various tissues,including integument,wing pads,foregut,midgut,hindgut,Malpighian tubules and fat body,and the amounts of LmLRP2 transcripts decreased gradually in the early stages and then increased in the late stages before ecdysis during the nymphal developmental stage.Fluorescence immunohistochemistry revealed that the LmLRP2 protein is mainly located in cellular membranes of the midgut and hindgut.Using RNAi to silence LmLRP2 caused molting defects in nymphs(more than 60％),and the neutral lipid was found to accumulate in the midgut and surface of the integument,but not in the fat body,of dsLmLRP2-treated nymphs.The results of a lipidomics analysis showed that the main components of lipids(diglyceride and triglyceride)were significantly increased in the midgut,but decreased in the fat body and hemolymph.Furthermore,the content of total triglyceride was significantly increased in the midgut,but markedly decreased in the fat body and hemolymph in dsLmLRP2-injected nymphs.Our results indicate that LmLRP2 is located in the cellular membranes of midgut cells,and is required for lipid export from the midgut to the hemolymph and fat body in locusts.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Prescreening of large-effect markers with multiple strategies improves the accuracy of genomic prediction

    Keanning LiBingxing AnMang LiangTianpeng Chang...
    1634-1643页
    查看更多>>摘要：Presently,integrating multi-omics information into a prediction model has become a ameliorate strategy for genomic selection to improve genomic prediction accuracy.Here,we set the genomic and transcriptomic data as the training population data,using BSLMM,TWAS,and eQTL mapping to prescreen features according to |Aβb|＞0,top 1％of phenotypic variation explained(PVE),expression-associated single nucleotide polymorphisms(eSNPs),and egenes(false discovery rate(FDR)＜0.01),where these loci were set as extra fixed effects(named GBLUP-Fix)and random effects(GFBLUP)to improve the prediction accuracy in the validation population,respectively.The results suggested that both GBLUP-Fix and GFBLUP models could improve the accuracy of longissimus dorsi muscle(LDM),water holding capacity(WHC),shear force(SF),and pH in Huaxi cattle on average from 2.14 to 8.69％,especially the improvement of GFBLUP-TWAS over GBLUP was 13.66％for SF.These methods also captured more genetic variance than GBLUP.Our study confirmed that multi-omics-assisted large-effects loci prescreening could improve the accuracy of genomic prediction.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Transcriptome-based analysis of key signaling pathways affecting the formation of primordial germ cell in chickens

    Ying DingQiong ZhiQisheng ZuoKai Jin...
    1644-1657页
    查看更多>>摘要：Bone morphogenetic protein 4(BMP4)can induce the formation of chicken primordial germ cells(PGCs)in vitro;however,its regulatory mechanism in poultry remains unknown.This study aimed to use RNA-seq to analyze PGCs in chicken embryos and iPGCs induced by BMP4 in vitro,clarify the internal regulatory factors of PGCs,analyze the mechanism of the formation of PGCs,and lay a theoretical foundation for the further optimization of PGCs induction systems.Embryonic stem cells(ESCs),PGCs and iPGCs induced by BMP4 in vitro were collected.The transcriptional maps of the three cell types were studied using RNA-seq.The results showed 6,142 genes differentially expressed between PGCs and iPGCs,of which 2,728 were upregulated in iPGCs and 3,414 were downregulated in iPGCs.Compared to that in ESCs,BMP4 was significantly upregulated in PGCs and iPGCs.KEGG results showed that both the TGF-β and Wnt signaling pathways were activated during the formation of PGCs in vitro and in vivo,and the activation was more significant during iPGCs induced by BMP4.The expression of Nodal,an inhibitory factor of TGF-β signaling,was significantly decreased in PGCs and iPGCs,but was not expressed in iPGCs,which further supports our conclusion.Additionally,the Lysosome and PI3K-AKT signaling pathways were significantly enriched in PGCs and iPGCs,respectively.Further,transmission electron microscopy(TEM)results showed that the number of autolysosomes was significantly higher after the addition of BMP4,which is consistent with the KEGG results.Furthermore,the number of PGCs was significantly reduced after ATG14 was interfered in vivo and in vitro.In conclusion,this study screened out the key signaling pathways during the formation of PGCs,aiming to provide help for enriching the mechanism network regulating PGCs formation in chicken and laying a theoretical foundation for further improving the efficiency of inducing PGCs in vitro.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    A novel live attenuated vaccine candidate protects chickens against subtype B avian metapneumovirus

    Lingzhai MengMengmeng YuSuyan WangYuntong Chen...
    1658-1670页
    查看更多>>摘要：Avian metapneumovirus(aMPV)is a highly contagious pathogen that causes acute upper respiratory tract diseases in chickens and turkeys,resulting in serious economic losses.Subtype B aMPV has recently become the dominant epidemic strain in China.We developed an attenuated aMPV subtype B strain by serial passaging in Vero cells and evaluated its safety and efficacy as a vaccine candidate.The safety test showed that after the 30th passage,the LN16-A strain was fully attenuated,as clinical signs of infection and histological lesions were absent after inoculation.The LN16-A strain did not revert to a virulent strain after five serial passages in chickens.The genomic sequence of LN16-A differed from that of the parent wild-type LN16(wtLN16)strain and had nine amino acid mutations.In chickens,a single immunization with LN16-A induced robust humoral and cellular immune responses,including the abundant production of neutralizing antibodies,CD4+T lymphocytes,and the Th1(IFN-y)and Th2(IL-4 and IL-6)cytokines.We also confirmed that LN16-A provided 100％protection against subtype B aMPV and significantly reduced viral shedding and turbinate inflammation.Our findings suggest that the LN16-A strain is a promising live attenuated vaccine candidate that can prevent infection with subtype B aMPV.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Optimized nitrogen application for maximizing yield and minimizing nitrogen loss in film mulching spring maize production on the Loess Plateau,China

    Qilong SongJie ZhangFangfang ZhangYufang Shen...
    1671-1684页
    查看更多>>摘要：Excessive use of N fertilizers(driven by high-yield goals)and its consequent environmental problems are becoming increasingly acute in agricultural systems.A 2-year field experiment was conducted to investigate the effects of three N application methods(application of solid granular urea once(OF)or twice(TF),application of solid granular urea mixed with controlled-release urea once(MF)),and six N rates(0,60,120,180,240,and 300 kg N ha-1)on maize yield,economic benefits,N use efficiency,and soil N balance in the maize(Zea mays L.)film mulching system on the Loess Plateau,China.The grain yield and economic return of maize were significantly affected by the N rate and application method.Compared with the OF treatment,the MF treatment not only increased the maize yield(increased by 9.0-16.7％)but also improved the economic return(increased by 10.9-25.8％).The agronomic N use efficiency(NAE),N partial factor productivity(NPFP)and recovery N efficiency(NRE)were significantly improved by 19.3-66.7,9.0-16.7 and 40.2-71.5％,respectively,compared with the OF treatment.The economic optimal N rate(EONR)of the OF,TF,and MF was 145.6,147.2,and 144.9 kg ha-1 in 2019,and 206.4,186.4,and 146.0 kg ha-1 in 2020,respectively.The apparent soil N loss at EONR of the OF,TF,and MF were 97.1-100.5,78.5-79.3,and 50.5-68.1 kg ha-1,respectively.These results support MF as a one-time N application method for delivering high yields and economic benefits,with low N input requirements within film mulching spring maize system on the Loess Plateau.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Soybean(Glycine max)rhizosphere organic phosphorus recycling relies on acid phosphatase activity and specific phosphorus-mineralizing-related bacteria in phosphate deficient acidic soils

    Qianqian ChenQian ZhaoBaoxing XieXing Lu...
    1685-1702页
    查看更多>>摘要：Bacteria play critical roles in regulating soil phosphorus(P)cycling.The effects of interactions between crops and soil P-availability on bacterial communities and the feedback regulation of soil P cycling by the bacterial community modifications are poorly understood.Here,six soybean(Glycine max)genotypes with differences in P efficiency were cultivated in acidic soils with long-term sufficient or deficient P-fertilizer treatments.The acid phosphatase(AcP)activities,organic-P concentrations and associated bacterial community compositions were determined in bulk and rhizosphere soils.The results showed that both soybean plant P content and the soil AcP activity were negatively correlated with soil organic-P concentration in P-deficient acidic soils.Soil P-availability affected the α-diversity of bacteria in both bulk and rhizosphere soils.However,soybean had a stronger effect on the bacterial community composition,as reflected by the similar biomarker bacteria in the rhizosphere soils in both P-treatments.The relative abundance of biomarker bacteria Proteobacteria was strongly correlated with soil organic-P concentration and AcP activity in low-P treatments.Further high-throughput sequencing of the phoC gene revealed an obvious shift in Proteobacteria groups between bulk soils and rhizosphere soils,which was emphasized by the higher relative abundances of Cupriavidus and Klebsiella,and lower relative abundance of Xanthomonas in rhizosphere soils.Among them,Cupriavidus was the dominant phoC bacterial genus,and it was negatively correlated with the soil organic-P concentration.These findings suggest that soybean growth relies on organic-P mineralization in P-deficient acidic soils,which might be partially achieved by recruiting specific phoC-harboring bacteria,such as Cupriavidus.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Straw mulching alters the composition and loss of dissolved organic matter in farmland surface runoff by inhibiting the fragmentation of soil small macroaggregates

    Shanshan CaiLei SunWei WangYan Li...
    1703-1717页
    查看更多>>摘要：Straw mulching is a widespread practice for reducing the soil carbon loss caused by erosion.However,the effects of straw mulching on dissolved organic matter(DOM)runoff loss from black soil are not well studied.How straw mulching affects the composition and loss of runoff DOM by changing soil aggregates remains largely unclear.Here,a straw mulching treatment was compared to a no mulching treatment(as a control)on sloping farmland with black soil erosion in Northeast China.We divided the soil into large macroaggregates(＞2 mm),small macroaggregates(0.25-2 mm),and microaggregates(＜0.25 mm).After five rain events,the effects of straw mulching on the concentration(characterized by dissolved organic carbon(DOC))and composition(analyzed by fluorescence spectroscopy)of runoff and soil aggregate DOM were studied.The results showed that straw mulching reduced the runoff amount by 54.7％.Therefore,although straw mulching increased the average DOC concentration in runoff,it reduced the total runoff DOM loss by 48.3％.The composition of runoff DOM is similar to that of soil,as both contain humic-like acid and protein-like components.With straw mulching treatment,the protein-like components in small macroaggregates accumulated and the protein-like components in runoff declined with rain events.Fluorescence spectroscopy technology may help in understanding the hydrological paths of rain events by capturing the dynamic changes of runoff and soil DOM characteristics.A variation partitioning analysis(VPA)indicated that the DOM concentration and composition of microaggregates explained 68.2％of the change in runoff DOM from no mulching plots,while the change in runoff DOM from straw mulching plots was dominated by small macroaggregates at a rate of 55.1％.Taken together,our results demonstrated that straw mulching reduces the fragmentation of small macroaggregates and the loss of microaggregates,thus effecting DOM compositions in soil and reducing the DOM loss in runoff.These results provide a theoretical basis for reducing carbon loss in sloping farmland.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据