查看更多>>摘要：ACYL-CoA-BINDING PROTEINs(ACBPs)play crucial regulatory roles during plant response to hypoxia,but their molecular mechanisms remain poorly understood.Our study reveals that ACBP4 serves as a positive regulator of the plant hypoxia response by interacting with WRKY70,influencing its nucleocytoplasmic shuttling in Arabidopsis thaliana.Furthermore,we demon-strate the direct binding of WRKY70 to the ACBP4 promoter,resulting in its upregulation and suggesting a positive feedback loop.Addi-tionally,we pinpointed a phosphorylation site at Ser638 of ACBP4,which enhances submergence tolerance,potentially by facilitating WRKY70's nuclear shuttling.Surprisingly,a natural variation in this phosphorylation site of ACBP4 allowed A.thaliana to adapt to humid conditions during its historical demographic expansion.We further observed that both phosphorylated ACBP4 and oleoyl-CoA can impede the interaction between ACBP4 and WRKY70,thus promoting WRKY70's nuclear translocation.Finally,we found that the overexpression of orthologous BnaC5.ACBP4 and BnaA7.WRKY70 in Brassica napus increases submergence tolerance,indicating their func-tional similarity across genera.In summary,our research not only sheds light on the functional significance of the ACBP4 gene in hypoxia response,but also underscores its potential utility in breeding flooding-tolerant oilseed rape varieties.
查看更多>>摘要：Drought stress is a crucial environmental factor that limits plant growth,development,and productivity.Autophagy of misfolded proteins can help alleviate the damage caused in plants experiencing drought.However,the mechanism of autophagy-mediated drought tolerance in plants remains largely unknown.Here,we cloned the gene for a maize(Zea mays)selective autophagy receptor,NEXT TO BRCA1 GENE 1(ZmNBR1),and identified its role in the re-sponse to drought stress.We observed that drought stress increased the accumulation of autophago-somes.RNA sequencing and reverse transcription-quantitative polymerase chain reaction showed that ZmNBR1 is markedly induced by drought stress.ZmNBR1 overexpression enhanced drought tolerance,while its knockdown reduced drought tol-erance in maize.Our results established that ZmNBR1 mediates the increase in autophagosomes and autophagic activity under drought stress.ZmNBR1 also affects the expression of genes related to autophagy under drought stress.Moreover,we determined that BRASSINOSTEROID INSENSITIVE 1A(ZmBRI1a),a brassinosteroid receptor of the BRI1-like family,interacts with ZmNBR1.Phenotype analysis showed that ZmBRI1a negatively regulates drought tolerance in maize,and genetic analysis in-dicated that ZmNBR1 acts upstream of ZmBRI1a in regulating drought tolerance.Furthermore,ZmNBR1 facilitates the autophagic degradation of ZmBRI1a under drought stress.Taken together,our results reveal that ZmNBR1 regulates the expression of autophagy-related genes,thereby increasing auto-phagic activity and promoting the autophagic deg-radation of ZmBRI1a under drought stress,thus en-hancing drought tolerance in maize.These findings provide new insights into the autophagy degradation of brassinosteroid signaling components by the au-tophagy receptor NBR1 under drought stress.
查看更多>>摘要：Dendrocalamus brandisii(Munro)Kurz is a sympodial bamboo species with inimitable taste and flavorful shoots.Its rapid growth and use as high-quality material make this bamboo species highly valued for both food processing and wood applications.However,genome in-formation for D.brandisii is lacking,primarily due to its polyploidy and large genome size.Here,we assembled a high-quality genome for hexapioid D.brandisii,which comprises 70 chromosomes with a total size of 2,756 Mb,using long-read HiFi sequencing.Furthermore,we accurately separated the genome into its three constituent subgenomes.We used Oxford Nanopore Technologies long reads to construct a transcriptomic dataset covering 15 tissues for gene annotation to complement our genome assembly,revealing differential gene ex-pression and post-transcriptional regulation.By integrating metabolome analysis,we unveiled that well-balanced lignin formation,as well as abundant flavonoid and fructose contents,contribute to the superior quality of D.brandisii shoots.Integrating genomic,transcriptomic,and metabolomic datasets provided a solid foundation for enhancing bamboo shoot quality and developing efficient gene-editing techni-ques.This study should facilitate research on D.brandisii and enhance its use as a food source and wood material by providing crucial genomic resources.
查看更多>>摘要：It is generally accepted that jasmonate-ZIM do-main(JAZ)repressors act to mediate jasmonate(JA)signaling via CORONATINE-INSENSITIVE1(COI1)-mediated degradation.Here,we report a cryptic signaling cascade where a JAZ repressor,FvJAZ12,mediates multiple signaling inputs via phosphorylation-modulated subcellular trans-location rather than the COI1-mediated degradation mechanism in strawberry(Fragaria vesca).FvJAZ12 acts to regulate flavor metabolism and defense response,and was found to be the target of FvMPK6,a mitogen-activated protein kinase that is capable of responding to multiple signal stimuli.FvMPK6 phosphorylates FvJAZ12 at the amino acid residues S179 and T183 adjacent to the PY residues,thereby attenuating its nuclear accumulation and relieving its repression for FvMYC2,which acts to control the expression of lipoxygenase 3(FvLOX3),an important gene in-volved in JA biosynthesis and a diverse array of cellular metabolisms.Our data reveal a previously unreported mechanism for JA signaling and deci-pher a signaling cascade that links multiple signaling inputs with fruit trait development.
查看更多>>摘要：Most mechanistic details of chronologically or-dered regulation of leaf senescence are unknown.Regulatory networks centered on AtWRKY53 are crucial for orchestrating and integrating various senescence-related signals.Notably,AtWRKY53 binds to its own promoter and represses tran-scription of AtWRKY53,but the biological sig-nificance and mechanism underlying this self-repression remain unclear.In this study,we identified the VQ motif-containing protein AtVQ25 as a cooperator of AtWRKY53.The expression level of AtVQ25 peaked at mature stage and was specifically repressed after the onset of leaf senescence.AtVQ25-overexpressing plants and atvq25 mutants displayed precocious and delayed leaf senescence,respectively.Importantly,we identified AtWRKY53 as an interacting partner of AtVQ25.We determined that interaction between AtVQ25 and AtWRKY53 prevented AtWRKY53 from binding to W-box elements on the AtWRKY53 promoter and thus counteracted the self-repression of AtWRKY53.In addition,our RNA-sequencing data revealed that the AtVQ25-AtWRKY53 module is related to the salicylic acid(SA)pathway.Precocious leaf senescence and SA-induced leaf senescence in AtVQ25-overexpressing lines were inhibited by an SA pathway mutant,atsid2,and NahG transgenic plants;AtVQ25-overexpressing/atwrky53 plants were also insensitive to SA-induced leaf sen-escence.Collectively,we demonstrated that AtVQ25 directly attenuates the self-repression of AtWRKY53 during the onset of leaf senescence,which is substantially helpful for understanding the timing of leaf senescence onset modulated by AtWRKY53.
查看更多>>摘要：Yield improvement has long been an important task for soybean breeding in the world in order to meet the increasing demand for food and animal feed.miR396 genes have been shown to negatively regulate grain size in rice,but whether miR396 family members may function in a similar manner in soybean is un-known.Here,we generated eight soybean mu-tants harboring different combinations of ho-mozygous mutations in the six soybean miR396 genes through genome editing with clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas)12SF01 in the elite soybean cultivar Zhong-huang 302(ZH302).Four triple mutants(mir396aci,mir396acd,mir396adf,and mir396cdf),two quadruple mutants(mir396-abcd and mir396acfi),and two quintuple mu-tants(mir396abcdf and mir396bcdfi)were characterized.We found that plants of all the mir396 mutants produced larger seeds com-pared to ZH302 plants.Field tests showed that mir396adf and mir396cdf plants have sig-nificantly increased yield in growth zones with relatively high latitude which are suited for ZH302 and moderately increased yield in lower latitude.In contrast,mir396abcdf and mir396bcdfi plants have increased plant height and decreased yield in growth zones with rela-tively high latitude due to lodging issues,but they are suited for low latitude growth zones with increased yield without lodging problems.Taken together,our study demonstrated that loss-of-function of miR396 genes leads to sig-nificantly enlarged seed size and increased yield in soybean,providing valuable germ-plasms for breeding high-yield soybean.
查看更多>>摘要：Camptothecin is a complex monoterpenoid indole alkaloid with remarkable antitumor activity.Given that two C-10 modified camptothecin derivatives,topotecan and irinotecan,have been approved as potent anticancer agents,there is a critical need for methods to access other aromatic ring-functionalized congeners(e.g.,C-9,C-10,etc.).However,contemporary methods for chemical oxi-dation are generally harsh and low-yielding when applied to the camptothecin scaffold,thereby lim-iting the development of modified derivatives.Re-ported herein,we have identified four tailoring enzymes responsible for C-9 modifications of camptothecin from Nothapodytes tomentosa,via metabolomic and transcriptomic analysis.These consist of a cytochrome P450(NtCPT9H)which catalyzes the regioselective oxidation of campto-thecin to 9-hydroxycamptothecin,as well as two methyltransferases(NtOMT1/2,converting 9-hydroxycamptothecin to 9-methoxycamptothecin),and a uridine diphosphate-glycosyltransferase(NtUGT5,decorating 9-hydroxycamptothecin to 9-β-D-glucosyloxycamptothecin).Importantly,the critical residues that contribute to the specific cat-alytic activity of NtCPT9H have been elucidated through molecular docking and mutagenesis ex-periments.This work provides a genetic basis for producing camptothecin derivatives through meta-bolic engineering.This will hasten the discovery of novel C-9 modified camptothecin derivatives,with profound implications for pharmaceutical manufacture.
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