首页期刊导航|中医杂志（英文版）
期刊信息/Journal information
中医杂志（英文版）
中医杂志（英文版）

Cao Hong-xin

季刊

0255-2922

info@journaljtcm.com;jtcm@188.com

010-64050201

100700

北京东直门内南小街16号

中医杂志（英文版）/Journal Journal of Traditional Chinese MedicineCSCDSCI
正式出版
收录年代

    Efficacy of substances containing 3 types of active ingredients-saponins,flavones,and alkaloids in regulation of cytokines in autoimmune diseases a systematic review and Meta-analysis based on animal studies

    ZHU RuifangZHANG JunLYU YaruCHEN Yulu...
    417-426页
    查看更多>>摘要：OBJECTIVE:To investigate the efficacy of substances containing 3 types of active ingredients-saponins,flavones,and alkaloids on experimental animals with autoimmune diseases(AIDs).METHODS:The protocol for this systematic review and Meta-analysis was prospectively registered with PROSPERO(CRD42023395741).Searches were conducted in the China National Knowledge Infrastructure,Wanfang,Chinese Science and Technology Journals,China Biomedical,PubMed,Cochrane Library,and Embase databases to screen for animal studies investigating the therapeutic effects of saponins,flavones,or alkaloids on autoimmune diseases;consequently,corresponding data extraction tables were prepared.Systematic Review Centre for Laboratory Animal Experimentation was used to assess the risk of methodological bias in the included literature.RevMan 5.4 was used for the Meta-analysis on the 8 serum cytokines.RESULTS:A total of 31 studies were included,all of which were randomized controlled studies.Meta-analysis indicated that substances rich in saponins,flavones,and alkaloids reduced serum levels of interleukin(IL)-1β[standardized mean difference(SMD)=-1.94,95％confidence interval(CI)(-2.99,-0.90),P=0.0003],IL-6[SMD=-1.65,95％CI(-2.33,-0.97,)P＜0.000 01],IL-17[SMD=-2.41,95％CI(-3.61,-1.20),P＜0.0001],tumor necrosis factor(TNF)-α[SMD=-1.84,95％CI(-2.61,-1.06),P＜0.0001],and interferon(IFN)-γ[SMD=-1.54,95％CI(-2.43,-0.65),P=0.0007],but increased serum levels of IL-4[SMD=1.30,95％CI(0.15,2.44),P=0.03)and IL-10[SMD=2.05,95％CI(1.39,2.70),P＜0.000 01)in animal models.However,no significant regulatory effect of these three active components was observed on serum levels of IL-2[SMD=-0.63,95％CI(-1.82,0.57),P=0.30].CONCLUTIONS:Substances containing saponins,flavones,and alkaloids regulated the changes of immune-related cytokines,it may be a novel dietary substance to relieve and control autoimmune diseases in the future.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Chang'an decoction(肠安方)alleviates endoplasmic reticulum stress by regulating mitofusin 2 to improve colitis

    CHEN YoulanDING MingmingHUANG ChaoyuanZHENG Yiyuan...
    427-436页
    查看更多>>摘要：OBJECTIVE:To evaluate the protective effects of Chang'an decoction(肠安方,CAD)on colitis,and investigate the potential mechanisms underlying these effects from the perspectives of endoplasmic reticulum(ER)stress induced by mitofusin 2(MFN2).METHODS:The composition of CAD was identified by liquid chromatography-mass spectrometry technology.A mice model of dextran sulfate sodium(DSS)induced colitis was established and therapeutic effects of CAD were determined by detecting body weight,disease activity index,colon length and histopathological changes.Then,the expression levels of MFN2,ER stress markers and Nucleotide-binding domain and leucine-rich repeat protein3(NLRP3)relevant proteins were detected by polymerase chain reaction(PCR),Western blot,immunohistochemistry and immunofluorescence staining.Subsequently,knockdown and overexpression cell model were constructed to further investigate the underlying mechanism of MFN2 mediating ER stress and energy metabolism by PCR,Western blot,electron microscopy and reactive oxygen species(ROS)staining.Finally,inflammatory indicator and tight junction proteins were measured by PCR and immunofluorescence staining to evaluate the protective effects of CAD.RESULTS:Results showed that the indispensable regulatory role of MFN2 in mediating ER stress and mitochondrial damage was involved in the protective effects of CAD on colitis in mice fed with DSS.Network pharmacology analysis also revealed CAD may play a protective effect on colitis by affecting mitochondrial function.In addition,our data also suggested a causative role for MFN2 in the development of inflammatory responses and energy metabolic alterations by constructing a knockdown and overexpression cell model whereby alter proper ER-mitochondria interaction in Caco-2 cells.Furthermore,relative expression analyses of ER stress markers and NLRP3 inflammasome showed the onset of ER stress and activation of NLRP3 inflammasome,which is consistent with the above findings.In contrast,intervention of CAD could improve the mucosal barrier integrity and colonic inflammatory response effectively through inhibiting ER stress response mediated by MFN2.CONCLUSION:CAD could alleviate ER stress by regulating MFN2 to exert therapeutic effects on DSS-induced colitis,which might provide an effective natural therapeutic approach for the treatment of ulcerative colitis.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Transient receptor potential vanilloid 1 involved in the analgesic effects of total flavonoids extracted from Longxuejie(Resina Dracaenae Cochinchinensis)

    MO XiaoqiangCHEN YatingYIN QianCHEN Haibo...
    437-447页
    查看更多>>摘要：OBJECTIVE:To evaluate the analgesic effects of total flavonoids of Longxuejie(Resina Dracaenae Cochinchinensis)(TFDB)and explore the possible analgesic mechanism associated with transient receptor potential vanilloid 1(TRPV1).METHODS:Whole-cell patch clamp technique was used to observe the effects of TFDB on capsaicin-induced TRPV1 currents.Rat experiments in vivo were used to observe the analgesic effects of TFDB.Western blot and immunofluorescence experiments were used to test the change of TRPV1 expression in DRG neurons induced by TFDB.RESULTS:Results showed that TFDB inhibited capsaicin-induced TRPV1 receptor currents in acutely isolated dorsal root ganglion(DRG)neurons of rats and the half inhibitory concentration was(16.7±1.6)mg/L.TFDB(2-20 mg/kg)showed analgesic activity in the phase Ⅱ of formalin test and(0.02-2 mg per paw)reduced capsaicin-induced licking times of rats.TFDB(20 mg/kg)was fully efficacious on complete Freund's adjuvant(CFA)-induced inflammatory thermal hyperalgesia and capsaicin could weaken the analgesic effects.The level of TRPV1 expressions of DRG neurons was also decreased in TFDB-treated CFA-inflammatory pain rats.CONCLUSION:All these results indicated that the analgesic effect of TFDB may contribute to their modulations on both function and expression of TRPV1 channels in DRG neurons.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Optimized new Shengmai powder(优化新生脉散方)inhibits myocardial fibrosis in heart failure by regulating the rat sarcoma/rapidly accelerated fibrosarcoma/mitogen-activated protein kinase kinase/extracellular regulated protein kinases signaling pathway

    ZHANG ZeyuJIA ZhuangzhuangSONG YuweiZHANG Xuan...
    448-457页
    查看更多>>摘要：OBJECTIVE:Exploring the effect of Optimized New Shengmai powder(优化新生脉散方,ONSMP)on myocardial fibrosis in heart failure(HF)based on rat sarcoma(RAS)/rapidly accelerated fibrosarcoma(RAF)/mitogen-activated protein kinase kinase(MEK)/extracellular regulated protein kinases(ERK)signaling pathway.METHODS:Randomized 70 Sprague-Dawley rats into sham(n=10)and operation(n=60)groups,then established the HF rat by ligating the left anterior descending branch of the coronary artery.We randomly divided the operation group rats into the model,ONSMP[including low(L),medium(M),and high(H)dose],and enalapril groups.After the 4-week drug intervention,echocardiography examines the cardiac function and calculates the ratios of the whole/left heart to the rat's body weight.Finally,we observed the degree of myocardial fibrosis by pathological sections,determined myocardium collagen(COL)Ⅰ and COL Ⅲ content by enzyme-linked immunosorbent assay,detected the mRNA levels of COL Ⅰ,COL Ⅲ,α-smooth muscle actin(α-SMA),and c-Fos proto-oncogene(c-Fos)by universal real-time,and detected the protein expression of p-RAS,p-RAF,p-MEK1/2,p-ERK1/2,p-ETS-like-1 transcription factor(p-ELK1),p-c-Fos,α-SMA,COL Ⅰ,and COL Ⅲ by Western blot.RESULTS:ONSMP can effectively improve HF rat's cardiac function,decrease cardiac organ coefficient,COL volume fraction,and COL Ⅰ/Ⅲ content,down-regulate the mRNA of COL Ⅰ/Ⅲ,α-SMA and c-Fos,and the protein of p-RAS,p-RAF,p-MEK1/2,p-ERK1/2,p-ELK1,c-Fos,COL Ⅰ/Ⅲ,and α-SMA.CONCLUSIONS:ONSMP can effectively reduce myocardial fibrosis in HF rats,and the mechanism may be related to the inhibition of the RAS/RAF/MEK/ERK signaling pathway.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Huoxue Jiedu Huayu recipe(活血解方)inhibits macrophage-secreted vascular endothelial growth factor-a on angiogenesis and alleviates renal fibrosis in the contralateral kidneys of unilateral ureteral obstruction rats

    GAO XiaomengQIANG PanpanCHANG JingyueFAN Lili...
    458-467页
    查看更多>>摘要：OBJECTIVE:To elucidate the mechanism by which Huoxue Jiedu Huayu recipe(活血解毒化瘀方,HJHR)regulates angiogenesis in the contralateral kidney of unilateral ureteral obstruction(UUO)rats and the mechanism by which it reduces of renal fibrosis.METHODS:Male Wistar rats were randomly divided into 4 groups:the sham group,UUO group(180 d of left ureter ligation),UUO plus eplerenone(EPL)group,and UUO plus HJHR group.After 180 d of oral drug administration,blood and contralateral kidneys were collected for analysis.Angiogenesis-and fibrosis-related indexes were detected.RESULTS:HJHR and EPL improved structural damage and renal interstitial fibrosis in the contralateral kidney and reduced the protein expression levels of α-smooth muscle actin(α-SMA),vimentin and collagen I.Moreover,these treatments could reduce the expression of vascular endothelial growth factor-A(VEGFA)by inhibiting the infiltration of macrophages.Furthermore,HJHR and EPL significantly reduced the expression of CD34 and CD105 by downregulating VEGFA production,which inhibited angiogenesis.Finally,the coexpressions of CD34,CD105 and α-SMA were decreased in the HJHR and EPL groups,indicating that endothelial-to-mesenchymal transition was inhibited.CONCLUSIONS:These findings confirm that HJHR alleviates contralateral renal fibrosis by inhibiting VEGFA-induced angiogenesis,encourage the use of HJHR against renal interstitial fibrosis and provide a theoretical basis for the clinical management of patients with CKD.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Acupotomy alleviates knee osteoarthritis in rabbit by regulating chondrocyte mitophagy via Pink1-Parkin pathway

    ZHU WentingGUO ChangqingDU MeiMA Yunxuan...
    468-477页
    查看更多>>摘要：OBJECTIVE:To investigate the effect of acupotomy,on mitophagy and the Pink1-Parkin pathway in chondrocytes from rabbits with knee osteoarthritis(KOA).METHODS:A KOA model was established via the modified Videman method.Rabbits were randomly divided into a control group(CON),KOA group and KOA+acupotomy group(Acu).Rabbits in the acupotomy group were subjected to acupotomy for 4 weeks after model establishment.The behavior of the rabbits before and after intervention was recorded.Cartilage degeneration was evaluated by optical microscopy and fluorescence microscopy.The level of mitophagy was evaluated by transmission electron microscopy,immunofluorescence and enzyme-linked immunosorbent assay(ELISA).The expression of phosphatase and tensin homolog(PTEN)-induced kinase 1(Pink1)-Parkin mitophagy pathway components was evaluated by immunofluorescence,Western blotting and real-time polymerase chain reaction.RESULTS:In rabbits with KOA,joint pain,mobility disorders and cartilage degeneration were observed,the Mankin score was increased,collagen type Ⅱ(Col-Ⅱ)expression was significantly decreased,mitophagy was inhibited,mitochondrial function was impaired,and factors associated with the Pink1-Parkin pathway were inhibited.Acupotomy regulated the expression of Pink1-Parkin pathway-related proteins,the mitophagy-related protein microtubule-associated protein-1 light chain-3,the translocase of the outer membrane,and the inner mitochondrial membrane 23;increased the colocalization of mitochondria and autophagosomes;promoted the removal of damaged mitochondria;restored mitochondrial adenosine-triphosphate(ATP)production;and alleviated cartilage degeneration in rabbits with KOA.CONCLUSIONS:Acupotomy played a role in alleviating KOA in rabbits by activating mitophagy in chondrocytes via the regulation of proteins that are related to the Pink1-Parkin pathway.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Investigation of the active ingredients and mechanism of Shuangling extract in dextran sulfate sodium salt induced ulcerative colitis mice based on network pharmacology and experimental verification

    CHU MengzhenWANG YuLIN ZhijianLyu Jintao...
    478-488页
    查看更多>>摘要：OBJECTIVE:To explore the pharmacodynamic effects and potential mechanisms of Shuangling extract against ulcerative colitis(UC).METHODS:The bioinformatics method was used to predict the active ingredients and action targets of Shuangling extract against UC in mice.And the biological experiments such as serum biochemical indexes and histopathological staining were used to verify the pharmacological effect and mechanism of Shuangling extract against UC in mice.RESULTS:The Shuangling extract reduced the levels of seruminterleukin-1β(IL-1β),tumor necrosis factor-a(TNF-N),interleukin-6(IL-6)and other inflammatory factors in UC mice and inhibited the inflammatory response.AKT Serine/threonine Kinase 1 and IL-6 may be the main targets of the anti-UC action of Shuangling extract,and the TNF signaling pathway,Forkhead box O signaling pathway and T-cell receptor signaling pathway may be the main signaling pathways.CONCLUSION:The Shuangling extract could inhibit the inflammatory response induced by UC and regulate intestinal immune function through multiple targets and multiple channels,which provided a new option and theoretical basis for anti-UC.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Exploring the multicomponent synergy mechanism of Zuogui Wan(左归丸)on postmenopausal osteoporosis by a systems pharmacology strategy

    FENG YanchenLIU YaliDANG XueLIN Zixuan...
    489-495页
    查看更多>>摘要：OBJECTIVE:To explore the multi-component synergistic mechanism of Zuogui Wan(左归丸,ZGW)in treating postmenopausal osteoporosis(PMOP).METHODS:The main components and target genes of ZGW were screened via the Traditional Chinese Medicine Systems Pharmacology(TCMSP).In addition,the target gene sets of PMOP were derived from the GeneCards and Online Mendelian Inheritance in Man databases.The search tool for recurring instances of neighbouring genes(STRING)11.0 software was used to analyze the interaction among intersecting genes.Cytoscape 3.6.1 software and the Matthews correlation coefficient(MCC)algorithm were used to screen the core genes.Fifty Sprague-Dawley female rats were randomly divided into the sham-operated(Sham)group and the four ovariectomized(OVX)subgroups.Rats subjected to Sham or OVX were administered with the vehicle(OVX,1 mL water/100 g weight),17β-estradiol(E2,50 μg·kg-1·d-1),and lyophilized powder of ZGW at a low dose of 2.3(ZGW-L)and high dose of 4.6(ZGW-H)g·kg-1·d-1 for three months.The bone density and bone strength were assessed using dual-energy X-ray and three-point bending tests,respectively.Furthermore,enzyme-linked immun-osorbent assay,Hematoxylin-eosin staining,and western blot analysis were used to determine the potential pharmacological mechanisms of action of ZGW in PMOP.RESULTS:A total of 117 active compounds of ZGW were screened from the TCMSP.Furthermore,108 intersecting genes of drugs and diseases were identified.Using STRING software and the MCC algorithm,ten core genes,including C-X-C chemokine living 8(CXCL8),C-C chemokine receptor type 2(CCR2),alpha-2a active receptor(ADRA2A),melatonin receptor type 1B(MTNR1B),and amyloid-beta A4 protein(APP),were identified.The anti-osteoporosis regulation network of ZGW was constructed using the Cytoscape software.The animal experiments demonstrated that ZGW groups significantly reduced the serum levels of β-C-terminal telopeptide of type Ⅰ collagen(β-CTX)and increased serum levels of bone-specific alkaline phosphatase(BALP)(P＜0.05,P＜0.01).The OVX group exhibited a significant decrease in bone mineral density and bone strength compared with the Sham group(P＜0.01).Moreover,treatment with ZGW resulted in increased trabecular thickness,improved arrangement of trabecular structure,and reduced empty bone lacunae.Furthermore,treatment with ZGW significantly increased the protein expression of CXCL8,ADRA2A,and CCR2(P＜0.05,P＜0.01),and significantly decreased the protein expression of Runx2(P＜0.01).Furthermore,the ZGW and E2 groups demonstrated significantly increased BMD(P＜0.05,P＜0.01),improved bone strength(P＜0.05,P＜0.01),reduced expression of CXCL8,ADRA2A,and CCR2,and increased runt-related transcription factor 2 levels in bone tissue(P＜0.05,P＜0.01)compared with the OVX group.However,there were no significant differences in MTNR1B and APP expression among the groups.CONCLUSION:ZGW shows synergistic mechanisms in PMOP through multiple components,targets,and pathways.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Phytochemical analysis,antioxidant,acetylcholinesterase,and α-amylase inhibitors from Hippeastrum hybridum extract

    Naila SherMushtaq AhmedNadia Mushtaq
    496-504页
    查看更多>>摘要：OBJECTIVE:To investigate the effects of Hippeastrum hybridum(HH)as a free radical scavenger,and an inhibitor of the two enzymes i-e Alpha-amylase(α-amylase)and acetylcholinesterase(AChE).METHODS:In this study,HH plant was preliminary analyzed for phytochemical screening and then tested for its antioxidant,anti-α-amylase,and anti-AChE efficiency via standard procedures.RESULTS:Phytochemical analysis shows the existence of different compounds;while Coumarins and quinones were absent.The total phenolic,flavonoid,and tannins content were found to be(78.52±0.69)mg GAE/g,(2.01±0.04)mg RUE/g,and(58.12±0.23)mg TAE/g of plant extract respectively.28.02％±0.02％alkaloid and 2.02％±0.05％saponins were present in the HH extract.The HH extract showed the anti-oxidant property with IC50(50％inhibition)of(151.01±0.13)(HH),(79.01±0.04)(Ascorbic acid)for ferric reducing,(91.48±0.13)(HH),(48.02±0.11)(Ascorbic acid)against Ammonium molybdenum,(156.02±0.31)(HH),(52.38±0.21)(Ascorbic acid)against DPPH,136.01±0.21(HH),52.02±0.31(Ascorbic acid)against H2O2,and 154.12±0.03(HH),(40.05±0.15)(Ascorbic acid)μg/mL against ABTS respectively.Statistical analysis indicated that HH caused a competitive type of inhibition of α-amylase(Vmax remained constant and Km increases from 10.65 to 84.37％)while Glucophage caused the un-competitive type of inhibition i-e both Km and Vmax decreased from 40.49 to 69.15％and 38.86 to 69.61％respectively.The Ki,(inhibition constant);Kl,(dissociation constant),Km,(Michaelis-Menten constant),and IC50 were found to be 62,364,68.1,and 38.08±0.22 for HH and 12,101.05,195,34.01±0.21 for Glucophage.Similarly,HH causes an anon-competitive type of inhibition of AChE i-e Km remains constant while Vmax decreases from 60.5％to 74.1％.The calculated Ki,Kl,Km,and IC50 were found to be 32,36.2,0.05,and 18.117±0.018.CONCLUSION:From the current results,it is concluded that HH extract contains bioactive compounds,and could be a good alternative to controlling oxidants,Alzheimer's and Type-Ⅱ diabetic diseases.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Identifying the geographical origin and processing technology of Moyao(Myrrh)on the basis of near-infrared spectroscopy combined with chemometrics

    XU NingningYAN GanmingXU FengjieDENG Linfeng...
    505-514页
    查看更多>>摘要：OBJECTIVE:To evaluate the quality of Moyao(Myrrh)in the identification of the geographical origin and processing of the products.METHODS:Raw Moyao(Myrrh)and two kinds of Moyao(Myrrh)processed with vinegar from three countries were identified using near-infrared(NIR)spectroscopy combined with chemometric techniques.Principal component analysis(PCA)was used to reduce the dimensionality of the data and visualize the clustering of samples from different categories.A classical chemometric algorithm(PLS-DA)and two machine learning algorithms[K-nearest neighbor(KNN)and support vector machine]were used to conduct a classification analysis of the near-infrared spectra of the Moyao(Myrrh)samples,and their discriminative performance was evaluated.RESULTS:Based on the accuracy,precision,recall rate,and F1 value in each model,the results showed that the classical chemometric algorithm and the machine learning algorithm obtained positive results.In all of the chemometric analyses,the NIR spectrum of Moyao(Myrrh)preprocessed by standard normal variation or Multivariate scattering correction combined with KNN achieved the highest accuracy in identifying the geographical origins,and the accuracy of identifying the processing technology established by the KNN method after first-order derivative pretreatment was the best.The best accuracy of geographical origin discrimination and processing technology discrimination were 0.9853 and 0.9706 respectively.CONCLUSIONS:NIR spectroscopy combined with chemometric technology can be an important tool for tracking the origin and processing technology of Moyao(Myrrh)and can also provide a reference for evaluations of its quality and the clinical use.
      原文链接:
    • NETL
    • NSTL
    • 万方数据