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海南医科大学学报（英文版）
海南医科大学学报（英文版）

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海南医科大学学报（英文版）/Journal Journal of Hainan Medical University
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    In vitro study on the antiviral activity of 9 extracts of traditional Chinese herbal medicine against the human respiratory syncytial virus

    HU Xiao-yuanYIN Fei-feiZHANG Jun-qingLI Yong-hu...
    1-7页
    查看更多>>摘要：Objective: To study the in vitro virucidal activity of 9 extracts of traditional Chinese herbal medicine (the water extracts of Evodia lepta, Clausena lansium, Clerodendrum cyrtophyllum, Callicarpa nudiflora, Nauclea officinalis and Elaeagnus gonyanthes, the alcohol extracts of Nauclea officinalis, Elaeagnus gonyanthes and Zanthoxylumarmatum)on human respiratory syncytial virus (HRSV). Methods: The cytotoxic effect of the extracts on cells was evaluated by a cell viability assay using the CCK-8 method, a concentration of the extracts with cell viability greater than 50％ was selected for the follow-up anti-HRSV effect assay, the 50％ effective concentration (EC50) was assessed by an in vitro cell infection model. Results: The EC50s of the water extract from Clerodendrum cyrtophyllum, Callicarpa nudiflora and Elaeagnus gonyanthes were 0.05 mg/mL, 0.03 mg/mL and 0.05 mg/mL, and the therapeutic index (TI) of them were 18.60, 21.67 and 56.80 respectively. Conclusion: The water extracts of Clerodendrum cyrtophyllum, Callicarpa nudiflora and Elaeagnus gonyanthes possess the activity of anti-HRSV virus.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Study on gene regulation mechanism of Qiliqiangxin capsule on myocardial fibrosis in myocardial infarction rats

    JI Xiao?diWU Ai?mingLV MengYANG Ding...
    8-13页
    查看更多>>摘要：Objective: To investigate the effect and possible mechanism of Qiliqiangxin capsule on myocardial fibrosis in rats with myocardial infarction(MI). Methods: The rat model of myocardial infarction was established by ligation of anterior descending branch of left coronary artery. The rats were randomly divided into model group, Qiliqiangxin capsule group, captopril group after operation. Rats that without ligation were set as parallel control group,namely, the sham group. Cardiac pathology was observed by hematoxylin eosin (HE) staining after 4 weeks of treatment. Masson staining was used to observe myocardial fibrosis and calculate collagen volume fraction (CVF). The miR‑133a and expression levels of transforming growth factor β1 (TGF‑β1), Smad 2, Smad 3, typeⅠcollagen (col‑Ⅰ), typeⅢ collagen (col‑Ⅲ) mRNA were examined by Real‑time PCR. Results: Compared with the sham group, myocardial cells in model group were disordered, CVF was significantly increased(P<0.01), the expression levels of col‑Ⅰ, col‑Ⅲ mRNA were increased(P<0.01); besides, the expression level of miR‑133a was significantly decreased (P<0.01), the expression levels of TGF‑β1, Smad 2 and Smad 3 mRNA were increased (P<0.05, P<0.01). Compared with the model group, the arrangement of myocardial cells in Qiliqiangxin capsule group and captopril group were orderly and CVF was significantly decreased(P<0.05); the expression level of miR‑133a was increased (P<0.05, P<0.01); the expression levels of TGF‑β1, Smad 2 and Smad 3 mRNA were significantly decreased (P<0.01). Conclusion: Qiliqiangxin capsule can improve myocardial infarction rat myocardial tissue fibrosis, its mechanism may be in related with the regulation on miR‑133a/TGF‑β1/Smads signal pathway at the genetic level.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Pharmacokinetics of Jiaotai pill self-microemulsion in insomnia rats

    BAI Dan-niWEI Xi-hongSHEN Meng-tingWANG Qing-wei...
    14-21页
    查看更多>>摘要：Objective: The pharmacokinetics and relative bioavailability of Jiaotai pill self-microemulsion were evaluated by investigating the blood concentration of Berberine, Coptisine, Palmatine and Jatrorrhizine in insomnia rats. Methods: Insomnia rat model was established by intraperitoneal injection of p-chlorophenylalanine (PCPA).The model rats were given Jiaotai pill self-microemulsion and Jiaotai pill suspension. The contents of Berberine, Coptisine, Palmatine and Jatrorrhizine in plasma at different times after administration were determined by UPLC-MS/MS, and calculate pharmacokinetic parameters. Results: Under the set chromatographic conditions, the linear relationship of the four components was good, and the precision, accuracy and stability meet the requirements of biological samples. After intragastric administration of Jiaotai pill self-microemulsion, The Cmax of Berberine, Coptisine, Palmatine and Jatrorrhizine were (412.68±28.45), (68.65±3.92), (34.06±3.13), (40.60±1.22) ng/mL, and AUC0-∞ were (672.70±72.55), (146.04±25.01), (71.49±18.67), (72.25±9.54) ng·mL-1·h-1, respectively. Compared with Jiaotai pill suspension, the Cmax, AUC0-t and AUC0-∞ of the four components in insomnia rats were significantly increased (P<0.01). Conclusion: Jiaotai pill self-microemulsionl can promote the absorption of effective components in insomnia rats and improve its bioavailability.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Correlation between IL-33/sST2 signaling pathway and patients with essential hypertensive left ventricular hypertrophy

    XING Bu-dianWEI TingLU Yuan-yuanLENG Jun-jie...
    22-26页
    查看更多>>摘要：Objective: To detect the levels of interleukin-33 (IL-33) and soluble ST2 (sST2) in peripheral blood of patients with essential hypertensive left ventricular hypertrophy, and to discusstheir correlation with patients with essential hypertensive left ventricular hypertrophy was further discussed. Methods: A total of 220 patients with essential hypertension treated in the Department of Cardiovascular Medicine of the First Affiliated Hospital of Bengbu Medical College were enrolled as the experimental group. According to left ventricular mass index (LVMI), patients with essential hypertension were divided into the non-left ventricular hypertrophy group (NLVH, n=108 cases) and the left ventricular hypertrophy group (LVH, n=112 cases). We used ELISA to detect the serum levels of IL-33 and sST2, the expression levels of IL-33 in peripheral blood lymphocytes of the NLVH group and the LVH group (60 cases each) were detected by Western blot, and the relationship between IL-33 and LVMI, a marker of left ventricular hypertrophic condition, was analyzed by Pearson. The relationship between IL-33, sST2 and left ventricular hypertrophy in essential hypertension was studied. Results: Compared with the NLVH group, the expression levels of IL-33 and sST2 in the LVH group were significantly increased. The results of Western blot showed that the expression level of IL-33 in the LVH group (1.07±0.08) was higher than that in the NLVH group (0.63±0.05) (P<0.05). Pearson correlation analysis showed that IL-33 was positively correlated with LVMI, sST2 was positively correlated with LVMI. Conclusion: The levels of IL-33 and sST2 in serum and the expression levels of IL-33 protein in peripheral blood lymphocytes are significantly increased in patients with hypertensive left ventricular hypertrophy, and the occurrence and development of essential hypertensive left ventricular hypertrophy may be related to IL-33 and sST2.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Design and characterization of a bi-functional bybrid antibacterial peptide LLM against Pseudomonas aeruginosa

    ZHANG Ze-jinYAO Hong-jiXUE Zhao-yiZHAN Yu-tong...
    27-32页
    查看更多>>摘要：Objective: To design a bifunctional antimicrobial peptide with high antibacterial activity and endotoxin neutralization against P.aeruginosa and explore its bactericidal properties. Methods: The neutralizing endotoxin peptides and antimicrobial peptide against P.aeruginosa were connected by a GGGS-linker to get a bi-functional bybrid peptide. Its structural parameter was tested by EMBOSS software. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and bactericidal kinetics against P.aeruginosa were determined. Its effect on endotoxin neutralization and hemolysis were also evaluated. Results: We designed and obtained a bybrid peptide LLM, which carried +8 positive charge with high activity against P.aeruginosa and neutralizing endotoxin. The MIC of LLM against P.aeruginosa CMCC10104 and P.aeruginosa ATCC 9027 were 2 and 4 μmol/L, and MBC/MIC equal 1. LLM showed rapid anti- P.aeruginosa effects and significantly neutralized the endotoxin released, and not exhibited hemolysis as high as 115 μmol/L (400 μg/mL).Conclusion: The MICs of LLM against P.aeruginosa were 2～4 μmol/L, it showed significant activity of anti-P. aeruginosa and neutralizing endotoxin, and could kill bacteria quickly,and did not show significant hemolytic under 115 μmol/L.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Screening and comprehensive analysis of key genes in liver hepatocellular carcinoma based on bioinformatics

    WANG RuiBAI Hao-tianLI Ya-lanYANG Jing...
    33-42页
    查看更多>>摘要：Objective: To search and analyze the related genes of liver hepatocellular carcinoma (LIHC) by using bioinformatics technology. Methods: Gene expression omnibus (GEO) was used to retrieve the entry of "Liver hepatocellular carcinoma", and GSE109903 chip data was downloaded. The differentially expressed genes in the control group and liver hepatocellular carcinoma group were screened by bioinformatics analysis. GO enrichment analysis, KEGG pathway analysis, differential gene expression analysis and visualization processing were performed for the differentially expressed genes; Protein interaction network analysis and visualization processing were used to screen core genes EEF1A1 and HK2 with strong correlation with liver hepatocellular carcinoma. GEPIA, Kaplan-Meier plotter, GeneMANIA and Timer 2.0 databases were used to analyze the differential expression, prognostic value and immune cell infiltration of key genes in LIHC. Results: A total of 1059 differentially expressed genes were screened, including 637 up-regulated genes and 872 downregulated genes. Functional analysis showed that differentially expressed genes were mainly involved in the process of positive transcription regulation, nucleosome function, chromatin function and RNA binding. Pathway analysis showed that differentially expressed genes were involved in systemic lupus erythematosus, alcoholism and RNA polymerase I promoter opening pathway. The analysis of differential gene expression showed that the drugs with similar gene characteristics were mainly CDC inhibitors, prostaglandins, serotonin receptor antagonists, BAF transcriptional repression inhibitors, tyrosine phosphatase inhibitors, etc; Protein interaction network analysis showed that the main genes associated with LIHC were EEF1A1, HK2, FAM38A and LAMB3; EEF1A1 and HK2 genes were further analyzed by GEPIA. The results showed that the expression of EEF1A1 and HK2 mRNA in LIHC tissues was higher than that in normal tissues, and was significantly correlated with the pathological stage, overall survival rate and disease-free survival rate of LIHC patients. EEF1A1 and HK2 may be potential prognostic biomarkers for LIHC patients. In addition, the functions of EEF1A1 and HK2 were mainly related to translation factor activity, molecular chaperone mediated autophagy and carbohydrate catabolism, and purine nucleoside diphosphate metabolism, respectively. Immunocyte infiltration showed that the expression of EEF1A1 and HK2 was significantly correlated with the infiltration of a variety of immune cells, including six types of immune cells: CD4+T cells, macrophages, neutrophils, B cells, CD8+T cells and dendritic cells. Conclusion: By screening differentially expressed genes, we can identify the key genes in the development of liver hepatocellular carcinoma, and screen potential prognostic biomarkers for the survival of patients with liver hepatocellular carcinoma. At the same time, this study provides new ideas and programs for clinical treatment of liver hepatocellular carcinoma.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Mechanism of Gan Dou Ling in improving liver fibrosis in Wilson disease based on network pharmacology and experimental verification

    LI Xiao-yunWANG HanSUN Lan-tingLI Xiang...
    43-49页
    查看更多>>摘要：Objective: To explore and verify the mechanism of Gan Dou Ling in improving liver fibrosis in Wilson disease (WD) by network pharmacology and copper loaded mice experiments. Methods: The main chemical components and corresponding gene targets of each drug in Gan Dou Ling were obtained by using TCMSP database. The database of gene mutation and disease related gene was searched through the GeneCards database, DrugBank database, PharmGKB database and the DisGeNET database. After the intersection of drug and disease target genes. The STRING website was used to analyze the protein-protein interaction degree of target genes, and import the data to Cytoscape software 38.2 to analyze protein interaction network . The GO databases and KEGG databases were obtained in R language for enrichment analysis. On this basis, Masson staining were used to observe the degree of liver fibrosis in copper loaded mouse model, and the results of network pharmacological analysis were verified by Western Blot (WB).Results: A total of 108 drug disease intersection genes were analyzed by network pharmacology. Through PPI network analysis, JUN was found to be the key genes. The enrichment analysis of KEGG pathway showed that MAPK signal pathway was the important potential target pathways. Animal experiments showed that Gan Dou Ling could reduce liver fibrosis and inhibit the phosphorylation of P38, JNK and C-JUN in copper loaded mice.Conclusion: Gan Dou Ling may achieve the effect of treating WD liver fibrosis by inhibiting P38/JNK signaling pathway.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Systematic review and meta-analysis on efficacy of traditional Chinese medicine in treatment of inflammatory factors in patients with post-stroke depression

    WANG Hui-yingGOU JinYANG Li-xuanGUO Rong-juan...
    50-59页
    查看更多>>摘要：Objective: To systematically evaluate the effect of traditional Chinese medicine on inflammatory factors in patients with post-stroke depression (PSD) and the efficacy of traditional Chinese medicine on PSD. Methods: Chinese databases (Sinomed, CNKI, VIP, Wanfang), PubMed, Cochrane Library, and Web of Science were searched. Randomized controlled trials of patients with PSD treated with traditional Chinese medicine were included. Quality evaluation was performed and Meta-analysis was performed using RevMan 5.4 software. Results: The results show that TCM therapy is superior to western medicine in reducing the level of TNF-α [MD=-4.13,95％ CI(-4.75,-3.51), P<0.00001], IL-6 [MD=-3.39,95％ CI(-3.90,-2.88),P<0.00001] and IL-8 [MD=-2.12,95％ CI(-2.49,-1.75),P<0.00001] in venous blood of PSD patients, and is superior to western medicine in reducing the score of HAMD [MD=-4.22,95％ CI(-5.37,-3.07),P<0.00001] and NIHSS [MD=-2.33,95％ CI(-3.43,-1.23),P<0.0001]. The combination of Chinese medicine and western medicine is superior to western medicine in reducing the level of TNF-α [MD=-10.61,95％ CI(-15.30,-5.91),P<0.00001]、IL-1 [MD=-3.75,95％ CI(-4.51,-2.99),P<0.00001]、IL-6 [MD=-3.83,95％ CI(-4.49,-3.16),P<0.0001]、IL-8 [MD=-9.41,95％ CI(-14.89,-3.92),P<0.00001] and Hcy [MD=-3.81,95％ CI(-4.62,-2.99),P<0. 00001] in venous blood of patients with PSD, and is superior to western medicine in reducing the score of HAMD [MD=-3.06,95％ CI(-3.39,-2.73),P<0.00001]、NIHSS [MD=-4.47,95％ CI(-7.55,-1.39),P=0.004] and HAMA [MD=-2.83,95％ CI(-3.47,-2.19),P<0.00001] and improving BI index. Three studies do not mention adverse reactions, and 37 cases in the remaining three studies show rash, tremor, dizziness and other adverse reactions. The incidence of adverse reactions in the experimental group was lower than that in the control group, and there were no serious adverse reactions. Conclusion: The results suggest that traditional Chinese medicine can reduce the level of some inflammatory factors in patients with PSD to a certain extent, and its efficacy is better than that of western medicine alone. Nevertheless, this study has some limitations, and with the increase of related trials in the future, the conclusions of the study need to be further discussed by adding more high-quality RCT studies.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Meta-analysis of the clinical efficacy of Liqi Huoxue drop pill in the treatment of angina pectoris in coronary artery disease

    HAN YiHAN Yu-boZOU Guo-liangSUI Yan-bo...
    60-67页
    查看更多>>摘要：Objective: To evaluate the efficacy of Liqi Huoxue dropping pills in the treatment of angina pectoris in coronary heart disease using the Meta-analysis system. Methods: Randomized controlled trials (RCT) on the treatment of angina pectoris in coronary artery disease with the combination of Western medicine with Liqi Huoxue drop pills were searched using computerized search databases, including English databases PubMed, Embase, Web of Science, The Cochrane Library and Chinese databases CNKI, VIP, WanFang Data and CBM from the time of establishment until February 1, 2022. Data extraction was performed independently by two investigators and the quality of the literature of the included studies was assessed. A meta-analysis of the included literature was performed using Revman 5.4 software. Results: Nine studies with a total of 982 patients were included. The results of Meta-analysis showed that the observation group, which adopted conventional treatment of Western medicine combined with Li qi huo xue dropping pills, was better than the control group, which was treated with conventional Western medicine alone. Clinical efficacy of the intervention group[R R=1.20,95％CI=(1.12,1.29),P<0.00001],Angina pectoris symptom treatment[RR=1.17,95％CI =(1.07,1.29),P=0.0010],Number of angina attacks [SMD=-2.26,95％CI(-4.10,-0.42),P=0.002],IL-6[MD=-4.65,95％CI(-6.91,-2.39),P<0.0001],IL-18[MD=-2.53,95％CI(-2.84,-2.22),P<0.00001],HS-CRP[MD=-0.08,95％CI(-1.81,-0.75),P<0.00001],and NO[M D=8.03,95％CI(0.93,15.14),P=0.03] were significantly different and better than the control group (western conventional treatment), and the differences were all statistically significant. Conclusion: Combining Liqi Huoxue drop pill with conventional Western medicine for patients with coronary angina can effectively improve the clinical efficacy, the efficacy of angina symptoms, the number of angina attacks, serum inflammatory indexes interleukin-6, interleukin-18, high-sensitivity C-reactive protein, and endothelial function NO indexes in patients with coronary angina, providing a reliable evidence-based basis for combining Liqi Huoxue drop pill with Western medicine for the treatment of coronary angina.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Study on TCM intervention of NF-κB signal pathway in the treatment of bronchial asthma

    LAI Hai-yanYI WeiDENG KunLONG Juan...
    68-72页
    查看更多>>摘要：Bronchial asthma (asthma for short) is a common chronic respiratory disease inchildren, which has adverse effects on children's physical and mental health. Nuclear factor κB (NF-κB) signaling pathway is an important transcription factor in airway inflammation, and plays an important role in airway inflammation and airway remodeling in asthma. Clinical studies have found that traditional Chinese medicine has positive effects in the treatment of asthma. Hence, this paper aims to review the literatures on the treatment of asthma through traditional Chinese medicine compound and single herbs and extracts by regulating the NF-κB signaling pathway for nearly a decade. It is found that traditional Chinese medicine can inhibit airway inflammation, relieve airway smooth muscle spasm and reduce airway resistance through the regulation of NF-κB signaling pathway, so as to play a key role in the treatment of asthma, and provide a scientific basis for the treatment of asthma with traditional Chinese medicine.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普